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WO2003020968A3 - Method for analyzing nucleic acid sequences and gene expression - Google Patents

Method for analyzing nucleic acid sequences and gene expression Download PDF

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Publication number
WO2003020968A3
WO2003020968A3 PCT/EP2002/009614 EP0209614W WO03020968A3 WO 2003020968 A3 WO2003020968 A3 WO 2003020968A3 EP 0209614 W EP0209614 W EP 0209614W WO 03020968 A3 WO03020968 A3 WO 03020968A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
gene expression
acid sequences
analyzing nucleic
acid molecules
Prior art date
Application number
PCT/EP2002/009614
Other languages
German (de)
French (fr)
Other versions
WO2003020968A2 (en
Inventor
Dimitri Tcherkassov
Original Assignee
Genovoxx Gmbh
Dimitri Tcherkassov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genovoxx Gmbh, Dimitri Tcherkassov filed Critical Genovoxx Gmbh
Priority to AU2002337030A priority Critical patent/AU2002337030A1/en
Publication of WO2003020968A2 publication Critical patent/WO2003020968A2/en
Publication of WO2003020968A3 publication Critical patent/WO2003020968A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for analyzing nucleic acid sequences and gene expression. The method is based on the detection of fluorescence signals of nucleotide molecules inserted into growing nucleic acid chains by means of a polymerase. The reaction occurs on a plane surface, wherein a plurality of individual nucleic acid molecules are immobilized on said surface. All said nucleic acid molecules are exposed to the same conditions so that a build-up reaction can simultaneously take place in all nucleic acid molecules.
PCT/EP2002/009614 2001-08-29 2002-08-28 Method for analyzing nucleic acid sequences and gene expression WO2003020968A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002337030A AU2002337030A1 (en) 2001-08-29 2002-08-28 Method for analyzing nucleic acid sequences and gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10142256 2001-08-29
DE10142256.3 2001-08-29

Publications (2)

Publication Number Publication Date
WO2003020968A2 WO2003020968A2 (en) 2003-03-13
WO2003020968A3 true WO2003020968A3 (en) 2003-12-31

Family

ID=7696956

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/009614 WO2003020968A2 (en) 2001-08-29 2002-08-28 Method for analyzing nucleic acid sequences and gene expression

Country Status (3)

Country Link
AU (1) AU2002337030A1 (en)
DE (1) DE10239504A1 (en)
WO (1) WO2003020968A2 (en)

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7297518B2 (en) 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US7541444B2 (en) 2002-08-23 2009-06-02 Illumina Cambridge Limited Modified nucleotides
US7566537B2 (en) 2001-12-04 2009-07-28 Illumina Cambridge Limited Labelled nucleotides
US7592435B2 (en) 2005-08-19 2009-09-22 Illumina Cambridge Limited Modified nucleosides and nucleotides and uses thereof
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US8691754B2 (en) 2003-09-22 2014-04-08 Bioarray Solutions, Ltd. Microparticles with enhanced covalent binding capacity and their uses
US8691594B2 (en) 1996-04-25 2014-04-08 Bioarray Solutions, Ltd. Method of making a microbead array with attached biomolecules
US8703652B2 (en) 2009-11-06 2014-04-22 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides
US9035035B2 (en) 2004-11-05 2015-05-19 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9127314B2 (en) 2002-08-23 2015-09-08 Illumina Cambridge Limited Labelled nucleotides
US9133511B2 (en) 2000-10-06 2015-09-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US9147037B2 (en) 2004-08-02 2015-09-29 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
US9251583B2 (en) 2002-11-15 2016-02-02 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
US9436088B2 (en) 2001-06-21 2016-09-06 Bioarray Solutions, Ltd. Un-supported polymeric film with embedded microbeads
US9469873B2 (en) 2006-09-28 2016-10-18 Illumina, Inc. Compositions and methods for nucleotide sequencing
US9528151B2 (en) 2006-12-01 2016-12-27 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US10722858B2 (en) 2013-03-15 2020-07-28 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
WO2003034029A2 (en) 2001-10-15 2003-04-24 Bioarray Solutions, Ltd. Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
NZ547492A (en) 2003-10-28 2009-12-24 Bioarray Solutions Ltd Optimization of gene expression analysis using immobilized capture probes of different lengths and densities
CN1882703B (en) * 2003-10-29 2011-07-06 佰尔瑞溶液有限公司 Multiplexed nucleic acid analysis by fragmentation of double-stranded DNA
CA2557177A1 (en) 2004-02-19 2005-09-01 Stephen Quake Methods and kits for analyzing polynucleotide sequences
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
CA2566806A1 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070196832A1 (en) * 2006-02-22 2007-08-23 Efcavitch J William Methods for mutation detection
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
EP3406736B1 (en) 2006-06-14 2022-09-07 Verinata Health, Inc. Methods for the diagnosis of fetal abnormalities
EP2940029B1 (en) 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
CN102858995B (en) 2009-09-10 2016-10-26 森特瑞隆技术控股公司 Targeting sequence measurement
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
CA2854832C (en) 2011-11-07 2023-05-23 Ingenuity Systems, Inc. Methods and systems for identification of causal genomic variants
JP6525872B2 (en) 2012-08-08 2019-06-05 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Increasing dynamic range to identify multiple epitopes in cells
GB2519255B (en) 2013-02-01 2016-01-06 Univ California Methods for genome assembly and haplotype phasing
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US9593373B2 (en) 2013-03-15 2017-03-14 Illumina Cambridge Limited Modified nucleosides or nucleotides
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US10450620B2 (en) 2013-11-07 2019-10-22 The Board Of Trustees Of The Leland Stanford Junior University Cell-free nucleic acids for the analysis of the human microbiome and components thereof
AU2014362322B2 (en) 2013-12-11 2021-05-27 The Regents For Of The University Of California Methods for labeling DNA fragments to recontruct physical linkage and phase
EP4219710A3 (en) 2014-08-01 2023-08-16 Dovetail Genomics, LLC Tagging nucleic acids for sequence assembly
EP4030437A1 (en) 2015-02-17 2022-07-20 Dovetail Genomics, LLC Nucleic acid sequence assembly
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
JP7300831B2 (en) 2015-10-19 2023-06-30 ダブテイル ゲノミクス エルエルシー Methods for Genome Assembly, Haplotype Phasing, and Target-Independent Nucleic Acid Detection
EP3420108A4 (en) 2016-02-23 2019-11-06 Dovetail Genomics LLC PRODUCTION OF PHASE READING ASSEMBLIES FOR GENOME ASSEMBLY AND PHASE OF HAPLOTYPE
CN109477101B (en) 2016-05-13 2022-11-18 多弗泰尔基因组学有限责任公司 Recovery of long-range linkage information from preserved samples
US20210371918A1 (en) 2017-04-18 2021-12-02 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
EP3642358A1 (en) 2017-06-21 2020-04-29 Bluedot LLC Systems and methods for identification of nucleic acids in a sample
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. Methods and systems for sample extraction
AU2019214956B2 (en) 2018-01-31 2025-06-26 Dovetail Genomics, Llc Sample prep for DNA linkage recovery
US11166996B2 (en) 2018-12-12 2021-11-09 Flagship Pioneering Innovations V, Inc. Anellovirus compositions and methods of use
CN114341638A (en) 2019-06-27 2022-04-12 多弗泰尔基因组学有限责任公司 Methods and compositions for proximity linking
CN110951852B (en) * 2019-11-25 2022-11-25 齐鲁工业大学 Single-base continuous extension flow type target sequencing method

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005183A1 (en) * 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
WO1993021340A1 (en) * 1992-04-22 1993-10-28 Medical Research Council Dna sequencing method
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
WO2000006770A1 (en) * 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
WO2002061126A2 (en) * 2001-01-30 2002-08-08 Solexa Ltd. The preparation of polynucleotide arrays
WO2002088382A2 (en) * 2001-04-27 2002-11-07 Genovoxx Gmbh Method for analysing nucleic acid chains

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1993005183A1 (en) * 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
WO1993021340A1 (en) * 1992-04-22 1993-10-28 Medical Research Council Dna sequencing method
WO2000006770A1 (en) * 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
WO2002061126A2 (en) * 2001-01-30 2002-08-08 Solexa Ltd. The preparation of polynucleotide arrays
WO2002061127A2 (en) * 2001-01-30 2002-08-08 Solexa Ltd. Arrayed polynucleotides and their use in genome analysis
WO2002088382A2 (en) * 2001-04-27 2002-11-07 Genovoxx Gmbh Method for analysing nucleic acid chains

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TONISSON N ET AL: "UNRAVELLING GENETIC DATA BY ARRAYED PRIMER EXTENSION", CLINICAL CHEMISTRY AND LABORATORY MEDICINE, WALTER DE GRUYTER UND CO, DE, vol. 38, no. 2, 2000, pages 165 - 170, XP001023733, ISSN: 1434-6621 *

Cited By (37)

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US8691594B2 (en) 1996-04-25 2014-04-08 Bioarray Solutions, Ltd. Method of making a microbead array with attached biomolecules
US9400259B2 (en) 1996-04-25 2016-07-26 Bioarray Solutions, Ltd. Method of making a microbead array with attached biomolecules
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9133511B2 (en) 2000-10-06 2015-09-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7297518B2 (en) 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US9436088B2 (en) 2001-06-21 2016-09-06 Bioarray Solutions, Ltd. Un-supported polymeric film with embedded microbeads
US9388463B2 (en) 2001-12-04 2016-07-12 Illumina Cambridge Limited Labelled nucleotides
US9410200B2 (en) 2001-12-04 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
US7566537B2 (en) 2001-12-04 2009-07-28 Illumina Cambridge Limited Labelled nucleotides
US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9410199B2 (en) 2002-08-23 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
US9121060B2 (en) 2002-08-23 2015-09-01 Illumina Cambridge Limited Modified nucleotides
US9127314B2 (en) 2002-08-23 2015-09-08 Illumina Cambridge Limited Labelled nucleotides
US7541444B2 (en) 2002-08-23 2009-06-02 Illumina Cambridge Limited Modified nucleotides
US9388464B2 (en) 2002-08-23 2016-07-12 Illumina Cambridge Limited Modified nucleotides
US9251583B2 (en) 2002-11-15 2016-02-02 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
US8691754B2 (en) 2003-09-22 2014-04-08 Bioarray Solutions, Ltd. Microparticles with enhanced covalent binding capacity and their uses
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides
US9147037B2 (en) 2004-08-02 2015-09-29 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
US9035035B2 (en) 2004-11-05 2015-05-19 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US7592435B2 (en) 2005-08-19 2009-09-22 Illumina Cambridge Limited Modified nucleosides and nucleotides and uses thereof
US9469873B2 (en) 2006-09-28 2016-10-18 Illumina, Inc. Compositions and methods for nucleotide sequencing
US9528151B2 (en) 2006-12-01 2016-12-27 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US10329607B2 (en) 2009-11-06 2019-06-25 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US9845497B2 (en) 2009-11-06 2017-12-19 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US11390918B2 (en) 2009-11-06 2022-07-19 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10494669B2 (en) 2009-11-06 2019-12-03 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US8703652B2 (en) 2009-11-06 2014-04-22 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10968479B2 (en) 2009-11-06 2021-04-06 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10982275B2 (en) 2009-11-06 2021-04-20 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US11098350B2 (en) 2009-11-06 2021-08-24 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US11597966B2 (en) 2009-11-06 2023-03-07 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US11384389B2 (en) 2009-11-06 2022-07-12 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US10722858B2 (en) 2013-03-15 2020-07-28 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples
US11161087B2 (en) 2013-03-15 2021-11-02 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples

Also Published As

Publication number Publication date
AU2002337030A1 (en) 2003-03-18
DE10239504A1 (en) 2003-04-24
WO2003020968A2 (en) 2003-03-13

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