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WO2003017754A1 - Plante du genre taxus modifiee, procede d'obtention et utilisation de co2 pour augmenter la concentration de metabolites secondaires dans une plante - Google Patents

Plante du genre taxus modifiee, procede d'obtention et utilisation de co2 pour augmenter la concentration de metabolites secondaires dans une plante Download PDF

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Publication number
WO2003017754A1
WO2003017754A1 PCT/ES2002/000393 ES0200393W WO03017754A1 WO 2003017754 A1 WO2003017754 A1 WO 2003017754A1 ES 0200393 W ES0200393 W ES 0200393W WO 03017754 A1 WO03017754 A1 WO 03017754A1
Authority
WO
WIPO (PCT)
Prior art keywords
concentration
weight
taxanes
plant species
leaves
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/ES2002/000393
Other languages
English (en)
Spanish (es)
Inventor
Robert Save Montserrat
Carmen Biel Loscos
Javier Sanchez Molino
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carburos Metalicos SA
Institut Recerca i Tecnologia Agroalimentaries IRTA
Original Assignee
Institut Recerca i Tecnologia Agroalimentaries IRTA
Sociedad Espanola de Carburos Metalicos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Recerca i Tecnologia Agroalimentaries IRTA, Sociedad Espanola de Carburos Metalicos SA filed Critical Institut Recerca i Tecnologia Agroalimentaries IRTA
Publication of WO2003017754A1 publication Critical patent/WO2003017754A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/02—Treatment of plants with carbon dioxide

Definitions

  • the present invention relates to a modified plant species of the genus Taxus and the method of obtaining it.
  • the present invention also relates to the use of carbon dioxide (C0 2 ) to increase the concentration of natural products from secondary metabolism in a plant species.
  • the modified plant species has levels in taxanes (especially paclitaxel, also known as taxol®) higher than those found in the unmodified species.
  • Carbon fertilizer in greenhouses is a well-known technique that is used to increase biomass in trees and, in agriculture, to increase fruit quality.
  • Taxol® the compound known as paclitaxel
  • Taxol® is the only diterpene amide taxane that has antitumor and antileukemic properties. Therefore, it is of great interest to obtain this therapeutic chemical agent for the clinical treatment of patients presenting with breast cancer.
  • the taxol® exists only in small quantities, of the order of 0.01% inside the bark, in the species of Tejo ⁇ Taxus).
  • taxol® could not be synthesized and even now, the most economical source of this compound still comes from the Pacific Yew (Taxus brevifolia). For this reason, large areas of Pacific Tejo forests have been destroyed in the northeastern United States in order to obtain this anticancer drug.
  • the paclitaxel compound is, therefore, a secondary metabolite of great interest for its therapeutic qualities in the treatment of cancer and, therefore, there is a need to increase its extraction without this being a detriment to nature.
  • the present invention relates to the method of obtaining said modified plant species.
  • the present invention relates to a modified plant species of the genus Taxus that, surprisingly, comprises a concentration of taxanes higher than that presented by a plant species of the genus Taxus unmodified.
  • plant species means any of the vegetative states that plants present in their natural state or when they are cultivated in vivo in greenhouses, excluding intermediate growth or propagation states used in this definition. In vitro culture techniques.
  • Modified plant species of the genus Taxus means any plant species of the genus Taxus that has been subjected to the conditions of the process of the invention.
  • the invention relates to a modified plant species of the genus Taxus having a bark with a concentration by weight of taxanes greater than the concentration by weight of said compounds in the bark of a plant species of the genus Taxus unmodified.
  • the concentration by weight of taxanes in the bark is equal to or greater, by more than 50%, to the concentration by weight of taxanes in the bark of an unmodified plant species of the genus Taxus.
  • the concentration by weight of taxanes in the bark is equal to or greater than, by more than 100%, the concentration by weight of taxanes in the bark of an unmodified plant species of the genus
  • the invention also relates to a species modified vegetable of the genus Taxus that has leaves with a concentration by weight of taxanes greater than the concentration by weight of said compound in the leaves of a plant species of the genus Taxus unmodified.
  • the concentration by weight of taxanes in the leaves is equal to or greater than, by more than 20%, the concentration by weight of taxanes in the leaves of an unmodified plant species of the genus Taxus.
  • the concentration by weight of taxanes in the leaves is equal or greater, by more than 25%, to the concentration by weight of taxanes in the leaves of an unmodified plant species of the genus Taxus.
  • the present invention also relates to the process for obtaining the modified plant species of the genus Taxus.
  • the dose of C0 2 ranges between 500 and 700 ppm and the water regime, preferably, is 100%.
  • secondary metabolite refers to any natural product from the secondary metabolism of a plant species.
  • the present invention also relates to the use of C0 2 to increase the concentration of secondary metabolites in a plant species.
  • the use C0 2 in the present invention does not cause an exclusive increase in biomass but, in addition, there is an increase in the concentration of secondary metabolites, some with therapeutic interest such as paclitaxel.
  • the invention also relates to the use of C0 2 to, surprisingly, increase the concentration by weight of secondary metabolites in the leaves, bark, stems, branches, flowers and roots of a
  • C0 2 causes an increase in the concentration by weight of secondary metabolites in the same or higher cortex, by more than one
  • the use of C0 2 causes an increase in the weight concentration of metabolites
  • the invention also relates to the use 30 of C0 2 to advantageously increase the concentration by weight of secondary metabolites in the leaves of a plant species.
  • C0 2 results in an increase in the metabolite concentration by weight Secondary in the leaves equal or higher, by more than 20%, to the concentration of secondary metabolites in the leaves before submitting them to the supply of C0 2 .
  • the use of C0 2 results in an increase in the concentration by weight of secondary metabolites in the leaves equal or greater, by more than 25%, to the concentration of secondary metabolites in the leaves before subjecting them to the supply of C0 2 .
  • the present invention also relates to the use of C0 2 in a dose larger than 350 ppm, preferably a dose between 500 and 700 ppm, to increase the concentration of secondary metabolites in a plant species.
  • Example 1 Obtaining a modified plant species of the genus Taxus.
  • Yews are irrigated by spraying twice at day with a nutrient solution comprising the nutrients: P 2 ⁇ 5 : K 2 0, in a ratio 1: 0.5: 1.5 and pH 6.5.
  • C0 2 was supplied at various levels (350, 500 and 700 ppm), and at each level of C0 2 different water regimes (50 and 100%, 400 and 800 m-) were supplied. year "1 respectively). Thus the yews were treated in six different conditions.
  • the application of C0 2 was carried out using conventional polyethylene pipes.
  • the source of C0 2 was a liquid C0 2 tank equipped with an evaporator to pass the C0 2 to the gas phase.
  • the dosage of C0 2 was carried out by means of a system composed of an infrared analyzer, an automaton and a series of solenoid valves that injected C0 2 depending on the orders of the automaton.
  • Example 2 Effects of enrichment with C0 2 on yew.
  • the procedure followed to analyze the levels of taxanes comprises the following stages:
  • the carbonic fertilizer does not promote 'significant differences in the relative growth rate (RGR) of all yew plant or its fractions.
  • the tree shows a positive tendency towards growth in the presence of higher levels of C0 2 during the two years.
  • plants grown at 700 ppm contain 28.8% more taxanes in 400 mm-years "" 1 and 49.36% more taxanes in 800 mm-years "1 in the leaves compared to those submitted to 350 ppm
  • FIGS 1 and 2 Relative growth rate (RGR) of dry leaf weight at 400 and 800 mm-year "1 , respectively.
  • FIGS 3 and 4 Relative growth rate (RGR) of the dry weight of stems at 400 and 800 mm-year “1 , respectively.
  • FIGS 5 and 6 Relative growth rate (RGR) of the dry root weight at 400 and 800 mm-year “1 , respectively.
  • Figures 7 and 8 Relative growth rate (RGR) of the total dry weight at 400 and 800 mm -year "1 , respectively.
  • Figure 9 Distribution of dry weight of leaves grown at three doses of C0 2 and two of water. The values are the average of five plants ⁇ standard error.
  • Figure 10 Distribution of dry weight of roots grown at three doses of C0 2 and two of water. The values are the average of five plants ⁇ standard error.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Environmental Sciences (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Epoxy Compounds (AREA)

Abstract

L'invention concerne une espèce végétale du genre Taxus modifiée en présence d'anhydride carbonique, et présentant une concentration en poids de taxanes supérieure à celle d'une espèce végétale du genre Taxus non modifiée. Le procédé de modification se base sur l'administration d'une dose d'anhydride carbonique supérieure à 350 ppm et consiste à soumettre les plantes à un régime hydrique de 50 à 100 %.
PCT/ES2002/000393 2001-08-23 2002-08-05 Plante du genre taxus modifiee, procede d'obtention et utilisation de co2 pour augmenter la concentration de metabolites secondaires dans une plante Ceased WO2003017754A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP200101951 2001-08-23
ES200101951A ES2183745B1 (es) 2001-08-23 2001-08-23 Planta de taxus m0dificada y procedimiento para su obtencion.

Publications (1)

Publication Number Publication Date
WO2003017754A1 true WO2003017754A1 (fr) 2003-03-06

Family

ID=8498775

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2002/000393 Ceased WO2003017754A1 (fr) 2001-08-23 2002-08-05 Plante du genre taxus modifiee, procede d'obtention et utilisation de co2 pour augmenter la concentration de metabolites secondaires dans une plante

Country Status (2)

Country Link
ES (2) ES2201933B1 (fr)
WO (1) WO2003017754A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103430740A (zh) * 2013-08-14 2013-12-11 江苏鼎钰生态农业科技有限公司 在盐碱地种植红豆杉的方法
CN104838948A (zh) * 2015-05-14 2015-08-19 平顶山学院 一种纯生态红豆杉的栽培方法
CN106550838A (zh) * 2015-09-29 2017-04-05 奉节县奎满农业开发有限公司 红豆杉的种植方法
CN106550837A (zh) * 2015-09-29 2017-04-05 奉节县奎满农业开发有限公司 红豆杉的育苗施肥方法
CN111492885A (zh) * 2020-05-14 2020-08-07 东北林业大学 红豆杉和无花果树混交林的营造方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3614387A1 (de) * 1986-04-28 1987-10-29 Klaus Brusius Verfahren und vorrichtung zur gesteuerten anreicherung der raumluft in einem gewaechshaus mit kohlendioxyd (co(pfeil abwaerts)2(pfeil abwaerts))
US4817332A (en) * 1983-10-28 1989-04-04 Mitsubishi Denki Kabushiki Kaisha Method of enhancing plant growth and apparatus for performing the same
EP0377232A1 (fr) * 1989-01-02 1990-07-11 Technica Entwicklungsgesellschaft mbH & Co. KG Procédé pour supporter la décomposition de portions élevées de CO2 dans l'atmosphère près du sol de plantes de culture
CN1149958A (zh) * 1995-10-30 1997-05-21 赵宝政 提高紫杉属植物针叶中紫杉醇含量的方法
WO1997031529A1 (fr) * 1996-02-27 1997-09-04 The United States Of America, Represented By The Secretary Of Agriculture Composes bioactifs
WO1997038571A1 (fr) * 1996-04-15 1997-10-23 Washington State University Research Foundation Compositions et procedes pour la biosynthese du taxol
WO1999000007A1 (fr) * 1995-08-22 1999-01-07 Grow International Corp. Procede de dormance comprimee pour augmenter la croissance de plantes

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ATA136687A (de) * 1987-05-27 1993-09-15 Technica Entwicklung Verfahren und vorrichtung zum beheben von durch naehrstoff-ueberschuss bedingten belastungen von kulturpflanzen
AP9901438A0 (en) * 1996-06-28 1999-03-31 The Agricultural Gas Company Pipeline utilization enhancement including carbondioxide gas transmission, distribution, and delivery technique.
US6060314A (en) * 1997-07-31 2000-05-09 Union Camp Corporation Utility-high carbon dioxide and light quality and quantity in woody plant propagation

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4817332A (en) * 1983-10-28 1989-04-04 Mitsubishi Denki Kabushiki Kaisha Method of enhancing plant growth and apparatus for performing the same
DE3614387A1 (de) * 1986-04-28 1987-10-29 Klaus Brusius Verfahren und vorrichtung zur gesteuerten anreicherung der raumluft in einem gewaechshaus mit kohlendioxyd (co(pfeil abwaerts)2(pfeil abwaerts))
EP0377232A1 (fr) * 1989-01-02 1990-07-11 Technica Entwicklungsgesellschaft mbH & Co. KG Procédé pour supporter la décomposition de portions élevées de CO2 dans l'atmosphère près du sol de plantes de culture
WO1999000007A1 (fr) * 1995-08-22 1999-01-07 Grow International Corp. Procede de dormance comprimee pour augmenter la croissance de plantes
CN1149958A (zh) * 1995-10-30 1997-05-21 赵宝政 提高紫杉属植物针叶中紫杉醇含量的方法
WO1997031529A1 (fr) * 1996-02-27 1997-09-04 The United States Of America, Represented By The Secretary Of Agriculture Composes bioactifs
WO1997038571A1 (fr) * 1996-04-15 1997-10-23 Washington State University Research Foundation Compositions et procedes pour la biosynthese du taxol

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* Cited by examiner, † Cited by third party
Title
DATABASE WPI Derwent World Patents Index; AN 2001-245320 *
LINDEN C.J. ET AL.: "Adv. in Biochem. Eng. Biotechnol.", vol. 72, 2001, ISBN: 3-540-41849-0, article "Gas concentration effects on secondary metabolite production by plant cell cultures", pages: 48 - 53 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103430740A (zh) * 2013-08-14 2013-12-11 江苏鼎钰生态农业科技有限公司 在盐碱地种植红豆杉的方法
CN104838948A (zh) * 2015-05-14 2015-08-19 平顶山学院 一种纯生态红豆杉的栽培方法
CN106550838A (zh) * 2015-09-29 2017-04-05 奉节县奎满农业开发有限公司 红豆杉的种植方法
CN106550837A (zh) * 2015-09-29 2017-04-05 奉节县奎满农业开发有限公司 红豆杉的育苗施肥方法
CN111492885A (zh) * 2020-05-14 2020-08-07 东北林业大学 红豆杉和无花果树混交林的营造方法
WO2021227168A1 (fr) * 2020-05-14 2021-11-18 东北林业大学 Procédé permettant de constituer une forêt mixte d'arbres de taxus chinensis et d'arbres de ficus carica
US11240955B2 (en) 2020-05-14 2022-02-08 Northeast Forestry University Method for constructing a mixed forest of yew trees and fig trees

Also Published As

Publication number Publication date
ES2183745B1 (es) 2004-06-01
ES2183745A1 (es) 2003-03-16
ES2201933B1 (es) 2005-02-01
ES2201933A1 (es) 2004-03-16

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