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WO2003014355A1 - Gene de nitrilase - Google Patents

Gene de nitrilase Download PDF

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Publication number
WO2003014355A1
WO2003014355A1 PCT/JP2002/007805 JP0207805W WO03014355A1 WO 2003014355 A1 WO2003014355 A1 WO 2003014355A1 JP 0207805 W JP0207805 W JP 0207805W WO 03014355 A1 WO03014355 A1 WO 03014355A1
Authority
WO
WIPO (PCT)
Prior art keywords
dna
nitrilase gene
nitrilase
probe
chromosomal dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2002/007805
Other languages
English (en)
Japanese (ja)
Inventor
Ken Watabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Soda Co Ltd
Original Assignee
Nippon Soda Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Soda Co Ltd filed Critical Nippon Soda Co Ltd
Priority to JP2003519484A priority Critical patent/JPWO2003014355A1/ja
Publication of WO2003014355A1 publication Critical patent/WO2003014355A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un gène de nitrilase identifié par extraction et purification de nitrilase d'Arthrobacter sp.NSSC104, par détermination des séquences d'acides aminés N-terminal et internes, par désignation d'une amorce sens et d'une amorce anti-sens, par PCR d'ADN chromosomal à l'aide de ces amorces, par marquage de l'ADN ainsi amplifié avec de la digoxygénine pour obtenir une sonde, et par criblage d'un transformant contenant l'ADN de recombinaison par hybridation sur colonies d'Escherichia coli avec un vecteur plasmidique transféré à l'intérieur, porteur d'un fragment d'ADN contenant un gène de nitrilase obtenu par digestion d'ADN chromosomal avec des enzymes de restriction et intégré, à l'aide de la sonde mentionnée ci-dessus.
PCT/JP2002/007805 2001-08-03 2002-07-31 Gene de nitrilase Ceased WO2003014355A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2003519484A JPWO2003014355A1 (ja) 2001-08-03 2002-07-31 ニトリラーゼ遺伝子

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001236020 2001-08-03
JP2001-236020 2001-08-03

Publications (1)

Publication Number Publication Date
WO2003014355A1 true WO2003014355A1 (fr) 2003-02-20

Family

ID=19067366

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/007805 Ceased WO2003014355A1 (fr) 2001-08-03 2002-07-31 Gene de nitrilase

Country Status (2)

Country Link
JP (1) JPWO2003014355A1 (fr)
WO (1) WO2003014355A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7198926B2 (en) 2003-05-08 2007-04-03 E. I. Du Pont De Nemours And Company Preparation of (E)- and (Z)-2-methyl-2-butenoic acids
JP2008022706A (ja) * 2006-07-18 2008-02-07 Gifu Univ ニトリラーゼおよびニトリラーゼを用いるカルボン酸の製造方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4390631A (en) * 1980-09-30 1983-06-28 Nitto Chemical Industry Co., Ltd. Process for producing bacteria having high nitrilase activity
JPH0799980A (ja) * 1993-10-05 1995-04-18 Japan Energy Corp ニトリラーゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、およびこれを含有する形質転換体によるニトリル類からカルボン酸の製造法
WO1997032030A1 (fr) * 1996-02-29 1997-09-04 Nippon Soda Co., Ltd. PROCESSUS DE PREPARATION D'ACIDES α-HYDROXY A L'AIDE D'UN MICRO-ORGANISME ET NOUVEAU MICRO-ORGANISME
JPH11341979A (ja) * 1998-06-01 1999-12-14 Nippon Soda Co Ltd ニトリラーゼ高生産性微生物菌体の製造法
JP2001136977A (ja) * 1999-09-02 2001-05-22 Showa Denko Kk ロドコッカス属細菌由来のニトリラーゼ遺伝子

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4390631A (en) * 1980-09-30 1983-06-28 Nitto Chemical Industry Co., Ltd. Process for producing bacteria having high nitrilase activity
JPH0799980A (ja) * 1993-10-05 1995-04-18 Japan Energy Corp ニトリラーゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、およびこれを含有する形質転換体によるニトリル類からカルボン酸の製造法
WO1997032030A1 (fr) * 1996-02-29 1997-09-04 Nippon Soda Co., Ltd. PROCESSUS DE PREPARATION D'ACIDES α-HYDROXY A L'AIDE D'UN MICRO-ORGANISME ET NOUVEAU MICRO-ORGANISME
JPH11341979A (ja) * 1998-06-01 1999-12-14 Nippon Soda Co Ltd ニトリラーゼ高生産性微生物菌体の製造法
JP2001136977A (ja) * 1999-09-02 2001-05-22 Showa Denko Kk ロドコッカス属細菌由来のニトリラーゼ遺伝子

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BARTEL B. ET AL.: "Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana", PROC. NATL. ACAD. SCI. USA, vol. 91, no. 14, 1994, pages 6649 - 6653, XP002959810 *
KOBAYASHI M. ET AL.: "Primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from Rhodococcus rhodochrous K22 and expression of its gene and identification of its active site residue", BIOCHEMISTRY, vol. 31, no. 37, 1992, pages 9000 - 9007, XP001062616 *
LAYH N. ET AL.: "Enrichment strategies for nitrile-hydrolysing bacteria", APPL. MICROBIOL. BIOTECHNOL., vol. 47, no. 6, 1997, pages 668 - 674, XP000881286 *
LEVI-SCHIL S. ET AL.: "Aliphatic nitrilase from a soil-isolated Comamonas trstosteroni sp.: gene cloning and overexpression, purification and primary structure", GENE, vol. 161, no. 1, 1995, pages 15 - 20, XP004042111 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7198926B2 (en) 2003-05-08 2007-04-03 E. I. Du Pont De Nemours And Company Preparation of (E)- and (Z)-2-methyl-2-butenoic acids
JP2008022706A (ja) * 2006-07-18 2008-02-07 Gifu Univ ニトリラーゼおよびニトリラーゼを用いるカルボン酸の製造方法

Also Published As

Publication number Publication date
JPWO2003014355A1 (ja) 2005-07-21

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