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WO2003098182A3 - Method for quantifying molecules - Google Patents

Method for quantifying molecules Download PDF

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Publication number
WO2003098182A3
WO2003098182A3 PCT/EP2003/005091 EP0305091W WO03098182A3 WO 2003098182 A3 WO2003098182 A3 WO 2003098182A3 EP 0305091 W EP0305091 W EP 0305091W WO 03098182 A3 WO03098182 A3 WO 03098182A3
Authority
WO
WIPO (PCT)
Prior art keywords
determining
molecules
proteins
quantifying molecules
quantifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/005091
Other languages
German (de)
French (fr)
Other versions
WO2003098182B1 (en
WO2003098182A2 (en
Inventor
Werner Stegmann
Michael Cahill
Andre Schrattenholz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ProteoSys AG
Original Assignee
ProteoSys AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10315932A external-priority patent/DE10315932A1/en
Application filed by ProteoSys AG filed Critical ProteoSys AG
Priority to AU2003233330A priority Critical patent/AU2003233330A1/en
Publication of WO2003098182A2 publication Critical patent/WO2003098182A2/en
Publication of WO2003098182A3 publication Critical patent/WO2003098182A3/en
Anticipated expiration legal-status Critical
Publication of WO2003098182B1 publication Critical patent/WO2003098182B1/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a method for determining the ratio of the protein frequencies of proteins of the same type in a first and at least one second sample, to a method for determining the concentration of stable isotopes in molecules and to a method for determining the incorporation rate of labelled substances into proteins.
PCT/EP2003/005091 2002-05-15 2003-05-15 Method for quantifying molecules Ceased WO2003098182A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003233330A AU2003233330A1 (en) 2002-05-15 2003-05-15 Method for quantifying molecules

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE10224803 2002-05-15
DE10224803.6 2002-05-15
DE10236716.7 2002-08-06
DE10236716 2002-08-06
DE10315932A DE10315932A1 (en) 2002-05-15 2003-04-02 Determining abundance ratios of similar proteins, useful for proteome analysis, based on labeling with stable isotopes and determination of isotope distribution
DE10315932.0 2003-04-02

Publications (3)

Publication Number Publication Date
WO2003098182A2 WO2003098182A2 (en) 2003-11-27
WO2003098182A3 true WO2003098182A3 (en) 2004-09-30
WO2003098182B1 WO2003098182B1 (en) 2004-12-02

Family

ID=29553742

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/005091 Ceased WO2003098182A2 (en) 2002-05-15 2003-05-15 Method for quantifying molecules

Country Status (2)

Country Link
AU (1) AU2003233330A1 (en)
WO (1) WO2003098182A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005181011A (en) * 2003-12-17 2005-07-07 Yoshio Yamauchi Method of analyzing protein
CN1314968C (en) * 2004-03-11 2007-05-09 复旦大学 Trace polypeptide or protein-enriched and its direct analyzing method
GB2413695B (en) * 2004-04-30 2009-01-21 Micromass Ltd Mass spectrometer
GB0409676D0 (en) 2004-04-30 2004-06-02 Micromass Ltd Mass spectrometer
EP1635178A3 (en) * 2004-09-14 2006-05-24 ProteoSys AG Breast cancer proteins
CN112683986B (en) * 2021-03-18 2021-06-15 裕菁科技(上海)有限公司 Natural isotope calibration curve method for quantifying target analyte in sample

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000067017A1 (en) * 1999-05-04 2000-11-09 The Rockefeller University Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000067017A1 (en) * 1999-05-04 2000-11-09 The Rockefeller University Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CAHILL MICHAEL A ET AL: "Analysis of relative isotopologue abundances for quantitative profiling of complex protein mixtures labelled with the acrylamide/D3-acrylamide alkylation tag system.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 17, no. 12, 2003, pages 1283 - 1290, XP009020320, ISSN: 0951-4198 (ISSN print) *
GENG M ET AL: "Signature-peptide approach to detecting proteins in complex mixtures", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 870, no. 1-2, February 2000 (2000-02-01), pages 295 - 313, XP004187331, ISSN: 0021-9673 *
GOSHE MICHAEL B ET AL: "Stable isotope-coded proteomic mass spectrometry.", CURRENT OPINION IN BIOTECHNOLOGY. ENGLAND FEB 2003, vol. 14, no. 1, February 2003 (2003-02-01), pages 101 - 109, XP009020301, ISSN: 0958-1669 *
HAMDAN MAHMOUD ET AL: "Modern strategies for protein quantification in proteome analysis: advantages and limitations.", MASS SPECTROMETRY REVIEWS. UNITED STATES 2002 JUL-AUG, vol. 21, no. 4, July 2002 (2002-07-01), pages 287 - 302, XP009020295, ISSN: 0277-7037 *
ONG SHAO-EN ET AL: "Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics", MOLECULAR AND CELLULAR PROTEOMICS, vol. 1, no. 5, May 2002 (2002-05-01), pages 376 - 386, XP009020302, ISSN: 1535-9476 *
SECHI SALVATORE ET AL: "Quantitative proteomics using mass spectrometry.", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 7, no. 1, February 2003 (2003-02-01), pages 70 - 77, XP009020300, ISSN: 1367-5931 (ISSN print) *
SMOLKA MARCUS ET AL: "Quantitative protein profiling using two-dimensional gel electrophoresis, isotope-coded affinity tag labeling, and mass spectrometry", MOLECULAR AND CELLULAR PROTEOMICS, vol. 1, no. 1, January 2002 (2002-01-01), pages 19 - 29, XP009020303, ISSN: 1535-9476 *
VOGT JOSEF A ET AL: "Protein abundance quantification in embryonic stem cells using incomplete metabolic labelling with 15N amino acids, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and analysis of relative isotopologue abundances of peptides.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 17, no. 12, 2003, pages 1273 - 1282, XP009020743, ISSN: 0951-4198 (ISSN print) *

Also Published As

Publication number Publication date
AU2003233330A1 (en) 2003-12-02
WO2003098182B1 (en) 2004-12-02
WO2003098182A2 (en) 2003-11-27
AU2003233330A8 (en) 2003-12-02

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