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WO2002038503A1 - Cartouche centrifuge et procede de capture, de concentration, et de collecte de micro-organismes - Google Patents

Cartouche centrifuge et procede de capture, de concentration, et de collecte de micro-organismes Download PDF

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Publication number
WO2002038503A1
WO2002038503A1 PCT/JP2001/009774 JP0109774W WO0238503A1 WO 2002038503 A1 WO2002038503 A1 WO 2002038503A1 JP 0109774 W JP0109774 W JP 0109774W WO 0238503 A1 WO0238503 A1 WO 0238503A1
Authority
WO
WIPO (PCT)
Prior art keywords
cartridge
filtration filter
filter
microorganisms
raw water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2001/009774
Other languages
English (en)
Japanese (ja)
Inventor
Motohiro Iseki
Isao Teramoto
Takeshi Yoshida
Osami Kato
Yoshie Tanizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Chemical Corp
Original Assignee
Mitsubishi Rayon Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Rayon Co Ltd filed Critical Mitsubishi Rayon Co Ltd
Priority to JP2002531049A priority Critical patent/JP4299537B2/ja
Priority to AU2002224021A priority patent/AU2002224021A1/en
Publication of WO2002038503A1 publication Critical patent/WO2002038503A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/02—Hollow fibre modules
    • B01D63/024—Hollow fibre modules with a single potted end
    • B01D63/0241—Hollow fibre modules with a single potted end being U-shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/16—Rotary, reciprocated or vibrated modules
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
    • C02F1/003—Processes for the treatment of water whereby the filtration technique is of importance using household-type filters for producing potable water, e.g. pitchers, bottles, faucet mounted devices
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
    • C02F1/444—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by ultrafiltration or microfiltration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/44—Cartridge types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/38—Treatment of water, waste water, or sewage by centrifugal separation
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2201/00—Apparatus for treatment of water, waste water or sewage
    • C02F2201/002—Construction details of the apparatus
    • C02F2201/006—Cartridges
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/04—Disinfection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0042—Investigating dispersion of solids
    • G01N2015/0053—Investigating dispersion of solids in liquids, e.g. trouble
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/019—Biological contaminants; Fouling

Definitions

  • the present invention relates to a cartridge for centrifugation for concentrating and recovering solids present in raw water, and a method for concentrating and recovering microorganisms using the same.
  • Centrifugation is used for various purposes, and it is widely known that centrifugation is performed for the purpose of concentrating and recovering solids present in water. At this time, if the amount of solids present in the water is small, it is necessary to perform high-concentration enrichment.
  • Such applications include testing and identifying microorganisms in the fields of food and medicine. Inspection and identification of microorganisms are performed while observing them with a microscope. However, when the amount of microorganisms in water is small, direct observation is difficult, so concentration must be performed.
  • Cryptosporidium is a protozoan parasite in livestock such as humans, cattle and pigs. Cryptosporidium is often mixed into tap water, causing large numbers of infected people. Cryptosporidium is contaminated in tap water because the parasites discharged into feces of patients and infected animals flow into surface water and contaminate the tap water source.
  • Cryptosporidium is excreted along with feces in an infectious form called an ostium. Since oocysts are wrapped in a dense membrane, they have very poor permeability of chemicals such as disinfectants, have extremely high resistance to chlorination, and are not suitable for chlorination in normal water treatment. Not activated.
  • oocysts are spherical with a diameter of about 5 m and are very small, it is extremely difficult to completely remove oocysts contained in raw water at the water treatment plant by current water purification treatment such as coagulation sedimentation and sand filtration. Have difficulty.
  • oocysts are very infectious Infect with one or several ingestions. Infection causes severe watery diarrhea, often fatal in immunocompromised patients.
  • the recovery rate is as low as about 30 ⁇ 1 ⁇ 2, and thus the detection error is large, and the following problems are pointed out to be improved.
  • microorganisms When collecting using a thread wound cartridge, microorganisms are likely to remain on the membrane surface during collection and extraction, and the trapped material penetrates into the inside, so that the recovery rate is significantly reduced and the microorganisms are collected. Requires a large amount of washing solution, resulting in a low concentration rate. While skilled techniques are required for extraction and concentration, skilled technologists being insufficient.
  • a hollow fiber membrane having a large membrane area per unit volume can be obtained.
  • the use of this technology allows for an improved method for capturing and recovering microorganisms, such as an increase in the amount of treated water, a reduction in filtration time, a reduction in the size of the apparatus, and a method for capturing and recovering protozoa existing in raw water in a short time.
  • a proposal has been made on a hollow fiber membrane module for capturing microorganisms.
  • a method of immersing the hollow fiber membrane in the washing liquid a method of ultrasonic cleaning with the hollow fiber membrane immersed in the washing liquid
  • a method of reversely passing a liquid through a hollow fiber membrane a method of immersing the hollow fiber membrane in a cleaning liquid to wash the fibers, and a method of cutting the hollow fiber membrane and then performing the cutting in the cleaning liquid.
  • the container and the membrane are integrally formed, if the cartridge is disposable in order to achieve high test accuracy, there is a problem that the inspection cost becomes high and the amount of waste increases.
  • an integrating flow meter and a valve may be installed in the piping between the faucet and the cartridge, and the integrating flow meter and the valve may be linked to automatically filter a specified amount of water. It is complicated, and especially on the raw water side of the cartridge, there is a problem that microorganisms and the like are caught on the integrating flow meter and valves, and as a result, the accuracy of collecting microorganisms is reduced.
  • the present invention has been made to solve such problems, and is a cartridge and a microorganism capture / concentration / recovery capable of capturing and concentrating microorganisms easily and inexpensively with high accuracy and without requiring skill in work.
  • the aim is to provide a method. Disclosure of the invention
  • a first aspect of the present invention is a cartridge that can be mounted on a centrifugal separator, having a raw water supply port and a filtered water outlet, wherein the cartridge is provided inside and between the raw water supply port and the filtered water outlet. It is characterized in that a filtration filter is provided between the cartridges, and the solid matter deposited on the filtration filter can be peeled off by centrifuging the cartridge.
  • the filtration filter is removably arranged because the number of disposal members can be reduced.
  • the member of the cartridge, to which the filtration filter is fixed, and the member having a portion where solids precipitate after centrifugal separation be detachable, because the operation can be easily performed.
  • the raw water supply port has a faucet mounting mechanism because connection and fixation are easy and work is simple.
  • the cartridge be self-supporting with the portion where solids settle down after centrifugation, so that an instrument such as a stand is not separately required for work.
  • the filtration filter is formed of a porous hollow fiber membrane because the membrane area of the cartridge body can be increased.
  • the filtration filter is any material selected from the group consisting of polyolefin, cellulose acetate, polysulfone, PAN, and polyvinylidene fluoride.
  • a second aspect of the present invention is a method for capturing, concentrating, and recovering microorganisms.
  • the raw water is filtered through the above-mentioned centrifugal separation cartridge. Then, by centrifuging without removing the filtration filter, the microorganisms accumulated on the filtration filter are separated from the filtration filter, settled on the bottom of the cartridge, and thereafter collected.
  • microorganisms can be collected with high accuracy, easily, and at a high rate.
  • FIG. 1 is a schematic sectional view showing an example of the cartridge for capturing and concentrating parasites of the present invention.
  • FIG. 2 is a schematic cross-sectional view showing another example of the cartridge for capturing and concentrating parasites of the present invention.
  • FIG. 3 is a schematic cross-sectional view showing another example of the protozoan capture and concentration cartridge of the present invention.
  • FIG. 4 is a schematic cross-sectional view showing another example of the protozoan capture / concentration cartridge of the present invention.
  • FIG. 5 is a schematic cross-sectional view showing another example of a protozoan capturing and enriching cartridge of the present invention. It is.
  • FIG. 6 is a schematic cross-sectional view showing another example of the protozoan capturing and concentrating cartridge of the present invention.
  • FIG. 7 is a schematic cross-sectional view showing one example of the cartridge for protozoan capture and concentration of the present invention.
  • FIG. 1 is a cross-sectional view schematically showing an example of the cartridge for centrifugation of the present invention, which has a raw water supply port 4 and a filtered water outlet 5, and has a raw water supply port 4 and a filtered water outlet inside the container 1.
  • 5 is a centrifugal separation cartridge having a filtration filter 6 between the cartridge 5.
  • the filtration filter 6 is preferably disposable because it is clogged by one concentration operation. Therefore, it is preferable to be arranged so as to be detachable from the cartridge.
  • the attaching and detaching method is not particularly limited as long as the liquid tightness of the primary side and the secondary side is maintained during filtration.
  • a filtration filter 16 can be assembled between the raw water supply port 4 and the filtered water outlet 5 inside the container 1 via a sealing material 7.
  • the assembling method may be such that the filter 6 is pressed into a fixing member having an inner diameter substantially the same as the outer diameter of the filter 6 and fixed.
  • an elastic member having an inner diameter slightly smaller than the outer diameter of the filtration filter 6 may be used, and the filtration filter 6 may be pushed in and fixed.
  • screw fitting may be used.
  • the cartridge is made detachable between a member to which the filtration filter 16 is fixed and a member having a portion where solids settle after centrifugation. It is preferable because there is no fear of re-mixing the precipitated solid matter when removing the filter 6.
  • the attaching / detaching method is not particularly limited as long as the liquid does not leak during centrifugation.
  • a cap 2 to which a filtration filter 6 is detachably fixed is provided, and the cap 2 is screwed into the container 1 so as not to leak through the sealing material 3 or the like. Can be assembled.
  • the attachment / detachment portion may have a substantially same inner / outer diameter and may be pushed in and fixed.
  • the elasticity having an inner diameter smaller than the other outer diameter may be used. It may be fixed by pushing it in using a flexible member.
  • the attachment / detachment site is not particularly limited. As shown in FIG. 4, the V-shaped member 15 of the container 1 may be divided from the cylindrical portion. It may be made possible to divide from.
  • a filter having an appropriate pore size may be selected according to the size of the microorganism to be captured.For example, a filter having a maximum pore size of 2 m or less is used for capturing cryptosporidium moss. Can be used.
  • the shape of the filter is not necessarily limited.
  • FIG. 2 shows an example using a bag-shaped filtration filter, which comprises a bag-shaped filtration filter 6 arranged on the surface of a hollow tubular porous body 17.
  • FIG. 3 shows an example in which a flat membrane filter is used, which is composed of a flat membrane filter 6 disposed on the surface of a porous body 17 having a hole for supplying raw water at the center. It is preferable to use a hollow fiber membrane for the filtration filter because a membrane area per filter volume can be widened and a larger amount of water can be filtered.
  • the upper limit of the membrane area per volume substantially occupied by a part of the filter is preferably 40 cm 2 / cm 3 , because if it is too large, the membrane will be too dense and the recovery efficiency will be poor. On the other hand, if it is too small, clogging is likely to occur, so the lower limit is preferably 4 cm 2 Z cm 3 .
  • a filtration filter made of a polyolefin-based material, a cellulose acetate-based material, a polysulfone-based material, a PAN-based material, or a polyvinylidene fluoride-based material is preferred because it is easily available.
  • a polyolefin-based material because it has the advantages of being tough and flexible, not being easily damaged during use, being inexpensive, and being incinerated at the time of disposal.
  • a plurality of porous hollow fiber membranes 8 are bent in a U-shape and fixed with a potting material 9 so that an open end is located on the filtered water outlet 5 side.
  • the raw water enters the container 1 through the raw water supply port 4, is filtered by the filter 6, and comes out from the filtered water outlet 5. Therefore, the microorganisms present in the raw water accumulate on the outer surface of the porous hollow fiber membrane 8 of the filtration filter 16.
  • Microorganisms deposited on the outer surface of the porous hollow fiber membrane 8 after passing the raw water through water are attached to a centrifugal separator and centrifuged as they are without removing the filtration filter. It is separated from the outer surface of the porous hollow fiber membrane 8 and concentrated at the bottom of the container 1.
  • the method for collecting the microorganism is not particularly limited.
  • a sediment take-out port may be provided at the bottom of the container 1 and taken out from the sediment.
  • the supernatant is extracted from the filtered water outlet 5 through the filtration filter 6, and then the member to which the filtration filter 6 is fixed is removed.
  • a method of collecting the microorganisms remaining at the bottom of the container 1 is more preferable because the process is simple.
  • the inside of the bottom of the container 1 is preferably tapered.
  • FIG. 5 is a cross-sectional view schematically showing another example of the centrifugal separation cartridge of the present invention.
  • a support member 14 for making the cartridge self-supporting is provided at the bottom of the container 1.
  • the shape of the support member 14 is not particularly limited as long as the cartridge can be made independent.
  • a hakama-shaped support member 14 surrounding the tapered portion can be provided at a height equal to or lower than the height of the tapered portion.
  • the support member 14 having a skirt shape may have a horizontal cross section equal to the outer circumference of the container 1 or, if the cartridge can stand alone, from the outer circumference of the container 1.
  • the horizontal cross section may be small or large.
  • the shape of the support member 14 can be any shape such as a circular cross-section, an ellipse, a polygon, and a star in a horizontal cross section.
  • a support member may be provided so that a plurality of legs are extended from the outer wall of the container to a height equal to or lower than the height of the tapered portion. I don't care. Also in this case, the shape or number of the feet is not particularly limited as long as the cartridge can stand alone.
  • a plate-shaped support member may be provided on the top of the tapered portion of the cartridge.
  • the size of the plate-shaped support member is not particularly limited as long as the cartridge can stand alone, and the horizontal cross section may be smaller or larger than the outer periphery of the container 1.
  • the shape is not particularly limited, and may be any shape such as a circle, an ellipse, a polygon, and a star.
  • the outer diameter of the bottom of the cartridge may be the same as the top, and the thickness of the bottom may be increased so that only the inside is tapered.
  • the cartridge When filtering raw water, it is preferable that the cartridge be easily connected and fixed in a stable state. For this reason, as shown in FIG. 6, the raw water supply port 4 of the cap 2 preferably has a faucet mounting mechanism to the faucet 13.
  • a mounting mechanism including a packing 12, a rotating ring 10 having a notch, and a mounting nut 11 can be used for simple and easy mounting. It can be fixed securely and is preferable.
  • the cartridge is attached to the tap 13 by the following operation.
  • Mounting nut 1 1 faucet Pass through 13 and then fit the rotating ring 10 into the end of the faucet 13, and attach the cartridge on which the packing 12 is set with the mounting nut 11.
  • the rotating ring 10 is securely fixed to the faucet 13 by tightening the faucet 13 due to the taper of the rotating ring 10.
  • the microorganisms deposited on the outer surface of the porous hollow fiber membrane 8 are removed by removing the cartridge 11 from the faucet 13 and centrifuging the cartridge without removing the filter. By being attached to the device and centrifuging, the porous hollow fiber membrane 8 is separated from the outer surface and concentrated at the bottom of the container 1.
  • the filtration flow rate is adjusted by adjusting the water flow pressure, for example, by adjusting the opening / closing degree of a valve.
  • the membrane is clogged by the progress of the filtration, even if the flow rate is adjusted first, the filtration flow rate will decrease if left untreated. For this reason, it is preferable to provide a constant flow mechanism 16 at the filtered water outlet 5 as shown in FIG.
  • the constant flow mechanism 16 is installed at the filtered water outlet 5 on the secondary side of the filtration filter 6. Install the cartridge securely so that it does not fall off when the cartridge is set in the centrifuge and centrifuged.
  • the constant flow mechanism 16 is installed so as to be integrated with the cartridge.However, a drain pipe (not shown) is connected to the filtered water outlet 5, and the constant flow mechanism 16 is connected to the drain pipe. It may be provided.
  • the type of the constant flow mechanism is not limited, the use of a constant flow valve that changes the resistance of the internal flow path using an elastic body such as rubber or a spring according to changes in the flow rate or pressure is excellent in handling, It is preferable because the price is low.
  • the size is preferably 5 cm or less as the outer diameter, more preferably 3 cm or less.
  • the filter 6 After the filtration, concentration by centrifugation, and subsequent recovery, the filter 6 is removed and discarded, and the other components are reused. At this time, it is preferable to sterilize other members in order to prevent contamination from the previous test from being carried into the next test and from contaminating the workers with microorganisms.
  • the sterilization method is not particularly limited, and can be performed by heat sterilization, sterilization by a chemical such as hypochlorous acid, formaldehyde, benzalkonium chloride, ethylene oxide gas, or sterilization by irradiation with ultraviolet rays or gamma rays.
  • a chemical such as hypochlorous acid, formaldehyde, benzalkonium chloride, ethylene oxide gas, or sterilization by irradiation with ultraviolet rays or gamma rays.
  • Sterilization by heating is preferably performed by heating in hot water at about 100 ° C. for about 1 minute to 10 minutes. Therefore, it is preferable that the material to be reused be a material having heat resistance that can withstand this.
  • heat-resistant plastic for example, polycarbonate resin, polyacetal resin, polyphenylene oxide resin, rigid It is preferable to use a plastic such as a polyvinyl chloride resin or a tetrafluoroethylene resin or an engineering plastic.
  • a solution containing 20 oocysts of purified and purified Cryptosporidium per liter was prepared and used in a 10 liter experiment.
  • experiments were performed for two cases, using distilled water as diluting water and using tap water.
  • a porous hollow fiber membrane (Polyethylene hollow fiber membrane EX540V manufactured by Mitsubishi Rayon Co., Ltd. (nominal maximum pore diameter 0.4 m, inner diameter 360 m, outer diameter 54 Oim)) is used as a filtration filter.
  • the effective fiber length of the hollow fiber membrane is 7 O mm (number: 528 hydrophilic porous hollow fiber membranes, 66 hydrophobic porous hollow fiber membranes), and the membrane area of the hollow fiber membrane is 705 cm 2 (Hollow fiber membrane outer diameter base) A filtration filter was manufactured.
  • the container and the cap were made of polycarbonate having heat resistance to hot water of 100 ° C.
  • the shape of the container can be directly attached to the centrifugal separator, and the bottom has a pointed shape so that the sediment after centrifugation can be collected efficiently.
  • the height of the cusp was 3.5 cm, and the outer diameter of the container was 6 cm.
  • the entire hollow fiber membrane is immersed in water, and the water in the container is hollow.
  • the amount of water remaining in the container when the membrane was filtered until it could not be filtered because it was exposed to the air was 25 mI.
  • the cap and the hollow fiber membrane filter, and the cap and the container were sealed with water using a sealing material.
  • a silicone rubber having heat resistance to hot water of 100 ° C. was used as a sealing material.
  • a faucet mounting mechanism consisting of a packing, a notched rotating ring, and a mounting nut so that the raw water flows into the raw water supply port of the protozoan capture / concentration cartridge, and to the other end of the faucet.
  • a faucet mounting mechanism consisting of a packing, a notched rotating ring, and a mounting nut so that the raw water flows into the raw water supply port of the protozoan capture / concentration cartridge, and to the other end of the faucet.
  • a hose was connected to the filtered water outlet so that the filtered water could flow out.
  • the hollow fiber membrane was connected to the filtered water outlet so that the raw water could be suction-filtered by a suction pump, and the hollow fiber membrane was exposed to air at a flow rate of about 60 O mm Hg at a flow rate of about 2 liters per minute. Suction filtration was performed until filtration became impossible.
  • the suction pump was connected again to the filtered water outlet via a hose, and the supernatant was sucked through the filter until the hollow fiber membrane was exposed to the air and could not be filtered.
  • X 100 was defined as the recovery rate.
  • the recovery when distilled water was used, the recovery was 90 ⁇ 1 ⁇ 2 or more. In addition, when tap water containing solids other than Cryptosporidium moss was used, the recovery was 80 ⁇ 5%.
  • the containers, caps, and sealing materials could be reused by boiling and disinfecting them with hot water for 5 minutes.
  • the cartridge for centrifugation and the method for capturing and concentrating microorganisms using the same is disassembled when the turbid component containing microorganisms present in the raw water is captured by a filtration filter, concentrated, and recovered. Since the enrichment process from capture to pre-recovery can be performed by a series of operations, it is easy to handle, and it is possible to recover a very small amount of a concentrated solution with a high concentration. It is possible to provide a technique that is simple in operation, does not require a skilled technique, and has high detection accuracy.
  • the cost of inspection can be reduced because it is possible to dispose of only a part of the filter after use.
  • the cartridge since the cartridge is self-supporting, it can be placed anywhere on a flat surface without the need for a stand or other device for setting up the cartridge, or the cartridge can be connected by installing a faucet mounting mechanism at the raw water intake. This eliminates the need for complicated work for fixing and extra equipment, and can greatly improve the handling.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Immunology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Centrifugal Separators (AREA)

Abstract

L'invention concerne une cartouche pouvant être montée sur un dispositif centrifuge, caractérisée en ce qu'elle comprend un orifice d'alimentation d'eau brute et un orifice de retrait de filtrat, et en ce qu'elle est dotée d'un filtre disposé entre lesdits orifice d'alimentation d'eau brute et orifice de retrait de filtrat, des protozoaires du type cryptosporidium pouvant être capturés efficacement et économiquement, et concentrés avec une précision élevée sans avoir recours à une compétence particulière. L'invention concerne également un procédé de capture, de concentration, et de collecte de protozoaires.
PCT/JP2001/009774 2000-11-09 2001-11-08 Cartouche centrifuge et procede de capture, de concentration, et de collecte de micro-organismes Ceased WO2002038503A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2002531049A JP4299537B2 (ja) 2000-11-09 2001-11-08 遠心分離用カートリッジ、微生物の捕捉回収用カートリッジ及び微生物捕捉濃縮回収方法
AU2002224021A AU2002224021A1 (en) 2000-11-09 2001-11-08 Centrifugal cartridge and method of capturing, concentrating and collecting microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000-341799 2000-11-09
JP2000341799 2000-11-09

Publications (1)

Publication Number Publication Date
WO2002038503A1 true WO2002038503A1 (fr) 2002-05-16

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/009774 Ceased WO2002038503A1 (fr) 2000-11-09 2001-11-08 Cartouche centrifuge et procede de capture, de concentration, et de collecte de micro-organismes

Country Status (3)

Country Link
JP (1) JP4299537B2 (fr)
AU (1) AU2002224021A1 (fr)
WO (1) WO2002038503A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102449460A (zh) * 2009-04-03 2012-05-09 3M创新有限公司 微生物浓集方法和装置
WO2017032845A1 (fr) * 2015-08-25 2017-03-02 Bbi-Biotech Gmbh Récipient d'analyse et procédé de filtration d'une suspension faisant appel à son utilisation
JP2022129561A (ja) * 2021-02-25 2022-09-06 水ｉｎｇ株式会社 クリプトスポリジウム分析用自動採水装置
CN116057378A (zh) * 2020-09-11 2023-05-02 富士胶片株式会社 浓缩器件、被检体液的浓缩方法、被检体液的检查方法、及检查试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4993956A (fr) * 1973-01-10 1974-09-06
JPH10314552A (ja) * 1997-05-15 1998-12-02 Mitsubishi Rayon Co Ltd 原虫捕捉用中空糸膜カートリッジ及び原虫の捕捉回収方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4993956A (fr) * 1973-01-10 1974-09-06
JPH10314552A (ja) * 1997-05-15 1998-12-02 Mitsubishi Rayon Co Ltd 原虫捕捉用中空糸膜カートリッジ及び原虫の捕捉回収方法

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102449460A (zh) * 2009-04-03 2012-05-09 3M创新有限公司 微生物浓集方法和装置
CN102449460B (zh) * 2009-04-03 2014-11-12 3M创新有限公司 微生物浓集方法和装置
WO2017032845A1 (fr) * 2015-08-25 2017-03-02 Bbi-Biotech Gmbh Récipient d'analyse et procédé de filtration d'une suspension faisant appel à son utilisation
US11541353B2 (en) 2015-08-25 2023-01-03 Bbi-Biotech Gmbh Container and method for filtering a suspension
CN116057378A (zh) * 2020-09-11 2023-05-02 富士胶片株式会社 浓缩器件、被检体液的浓缩方法、被检体液的检查方法、及检查试剂盒
EP4212873A4 (fr) * 2020-09-11 2024-06-05 FUJIFILM Corporation Dispositif de concentration, procédé de concentration d'une solution d'échantillon, procédé de test d'une solution d'échantillon et trousse de test
JP2022129561A (ja) * 2021-02-25 2022-09-06 水ｉｎｇ株式会社 クリプトスポリジウム分析用自動採水装置
JP7727393B2 (ja) 2021-02-25 2025-08-21 水ｉｎｇ株式会社 クリプトスポリジウム分析用自動採水装置

Also Published As

Publication number Publication date
JPWO2002038503A1 (ja) 2004-03-18
JP4299537B2 (ja) 2009-07-22
AU2002224021A1 (en) 2002-05-21

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