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WO2002034040A3 - Method for generating transgenic fish embryos using an episomal vector system - Google Patents

Method for generating transgenic fish embryos using an episomal vector system Download PDF

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Publication number
WO2002034040A3
WO2002034040A3 PCT/EP2001/012246 EP0112246W WO0234040A3 WO 2002034040 A3 WO2002034040 A3 WO 2002034040A3 EP 0112246 W EP0112246 W EP 0112246W WO 0234040 A3 WO0234040 A3 WO 0234040A3
Authority
WO
WIPO (PCT)
Prior art keywords
vector system
episomal vector
fish embryos
generating transgenic
transgenic fish
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/012246
Other languages
French (fr)
Other versions
WO2002034040A2 (en
Inventor
Alexander D Crawford
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MERMAID PHARMACEUTICALS GmbH
Original Assignee
MERMAID PHARMACEUTICALS GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MERMAID PHARMACEUTICALS GmbH filed Critical MERMAID PHARMACEUTICALS GmbH
Priority to AU2002221731A priority Critical patent/AU2002221731A1/en
Publication of WO2002034040A2 publication Critical patent/WO2002034040A2/en
Publication of WO2002034040A3 publication Critical patent/WO2002034040A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a novel method for generating transgenic fish embryos using an episomal vector system. In particular, the invention relates to the expression of transgenes in fish embryos or larvae using plasmids containing episomal replication elements derived from the Epstein-Barr episomal vector system.
PCT/EP2001/012246 2000-10-23 2001-10-23 Method for generating transgenic fish embryos using an episomal vector system Ceased WO2002034040A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002221731A AU2002221731A1 (en) 2000-10-23 2001-10-23 Method for generating transgenic fish embryos using an episomal vector system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24249900P 2000-10-23 2000-10-23
US60/242,499 2000-10-23

Publications (2)

Publication Number Publication Date
WO2002034040A2 WO2002034040A2 (en) 2002-05-02
WO2002034040A3 true WO2002034040A3 (en) 2002-11-28

Family

ID=22915010

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/012246 Ceased WO2002034040A2 (en) 2000-10-23 2001-10-23 Method for generating transgenic fish embryos using an episomal vector system

Country Status (2)

Country Link
AU (1) AU2002221731A1 (en)
WO (1) WO2002034040A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10252806A1 (en) * 2002-11-13 2004-06-03 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung Use of fish larvae as a screening model
DE602005027414D1 (en) * 2005-10-05 2011-05-19 Univ Milano Bicocca METHOD OF TRANSMITTING EPISOMAL VECTORS IN ANIMAL CELLS
WO2024151877A2 (en) * 2023-01-11 2024-07-18 Engage Biologics Inc. Non-viral expression systems and methods of use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056902A2 (en) * 1997-06-09 1998-12-17 Medical College Of Georgia Research Institute, Inc. Transgenic fish with tissue-specific expression

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056902A2 (en) * 1997-06-09 1998-12-17 Medical College Of Georgia Research Institute, Inc. Transgenic fish with tissue-specific expression

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ELBRECHT A ET AL: "EPISOMAL MAINTENANCE OF A BOVINE PAPILLOMA VIRUS VECTOR IN TRANSGENIC MICE", MOLECULAR AND CELLULAR BIOLOGY, WASHINGTON, DC, US, vol. 7, no. 3, 1 March 1987 (1987-03-01), pages 1276 - 1279, XP000673609, ISSN: 0270-7306 *
LINNEY E ET AL: "Transgene expression in zebrafish: A comparison of retroviral-vector and DNA-injection approaches.", DEVELOPMENTAL BIOLOGY. UNITED STATES 1 SEP 1999, vol. 213, no. 1, 1 September 1999 (1999-09-01), pages 207 - 216, XP001050547, ISSN: 0012-1606 *
WADE-MARTINS RICHARD ET AL: "Long-term stability of large insert genomic DNA episomal shuttle vectors in human cells.", NUCLEIC ACIDS RESEARCH, vol. 27, no. 7, 1 April 1999 (1999-04-01), pages 1674 - 1682, XP002204660, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2002034040A2 (en) 2002-05-02
AU2002221731A1 (en) 2002-05-06

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