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WO2002033104A3 - Systeme de regulation des genes a base d'antibiotique - Google Patents

Systeme de regulation des genes a base d'antibiotique Download PDF

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Publication number
WO2002033104A3
WO2002033104A3 PCT/IB2001/001963 IB0101963W WO0233104A3 WO 2002033104 A3 WO2002033104 A3 WO 2002033104A3 IB 0101963 W IB0101963 W IB 0101963W WO 0233104 A3 WO0233104 A3 WO 0233104A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibiotic
gene regulation
polypeptides
regulation system
based gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2001/001963
Other languages
English (en)
Other versions
WO2002033104A2 (fr
Inventor
Martin Fussenegger
James E Bailey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cistronics Cell Technology GmbH
Original Assignee
Cistronics Cell Technology GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cistronics Cell Technology GmbH filed Critical Cistronics Cell Technology GmbH
Priority to AU2002210802A priority Critical patent/AU2002210802A1/en
Publication of WO2002033104A2 publication Critical patent/WO2002033104A2/fr
Anticipated expiration legal-status Critical
Publication of WO2002033104A3 publication Critical patent/WO2002033104A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un nouveau système destiné à la régulation des gènes dans des cellules eucaryotes, ainsi que des procédés d'utilisation de celui-ci dans la production de protéines, dans le génie tissulaire et dans la thérapie génique. L'invention concerne, notamment, un nouveau système destiné à une expression génique régulée par antibiotique dans des cellules eucaryotes en fonction des séquences issues de promoteurs de résistance aux antibiotiques Actinomycètes, de polypeptides se liant à ceux-ci d'une manière sensible aux antibiotiques, et des nucléotides codant de tels polypeptides. De plus, l'invention concerne des nouveaux procédés sensibles de criblage d'antibiotiques candidats.
PCT/IB2001/001963 2000-10-20 2001-10-19 Systeme de regulation des genes a base d'antibiotique Ceased WO2002033104A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002210802A AU2002210802A1 (en) 2000-10-20 2001-10-19 Antibiotic-based gene regulation system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69362400A 2000-10-20 2000-10-20
US09/693,624 2000-10-20

Publications (2)

Publication Number Publication Date
WO2002033104A2 WO2002033104A2 (fr) 2002-04-25
WO2002033104A3 true WO2002033104A3 (fr) 2003-10-02

Family

ID=24785431

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/001963 Ceased WO2002033104A2 (fr) 2000-10-20 2001-10-19 Systeme de regulation des genes a base d'antibiotique

Country Status (2)

Country Link
AU (1) AU2002210802A1 (fr)
WO (1) WO2002033104A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3411483B1 (fr) * 2016-02-05 2022-07-06 PolyGene AG Système de régulation génique à base de glucocorticoïde

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000065080A1 (fr) * 1999-04-23 2000-11-02 Fussenegger, Martin Systeme de regulation genique par antibiotiques

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000065080A1 (fr) * 1999-04-23 2000-11-02 Fussenegger, Martin Systeme de regulation genique par antibiotiques

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AUGUST P R ET AL: "BIOSYNTHESIS OF THE ANSAMYCIN ANTIBIOTIC RIFAMYCIN: DEDUCTIONS FROM THE MOLECULAR ANALYSIS OF THE RIF BIOSYNTHETIC GENE CLUSTER OF AMYCOLATOPSIS MEDITERRANEI S699", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 5, no. 2, 13 February 1998 (1998-02-13), pages 69 - 79, XP001084972, ISSN: 1074-5521 *
BLANC V ET AL: "Molecular characterization and transcriptional analysis of a multdrug resistance gene cloned from the pristinamycin-producing organism, Streptomyces pristinaespiralis", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 17, no. 5, 1995, pages 989 - 999, XP008009341, ISSN: 0950-382X *
FOLCHER MARC ET AL: "A transcriptional regulator of a pristinamycin resistance gene in Streptomyces coelicolor.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 2, 12 January 2001 (2001-01-12), pages 1479 - 1485, XP002184465, ISSN: 0021-9258 *
KIM C-G ET AL: "3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 11, 13 March 1998 (1998-03-13), pages 6030 - 6040, XP002145258, ISSN: 0021-9258 *
SALAH-BEY K ET AL: "STRESS-ACTIVATED EXPRESSION OF A STREPTOMYCES PRISTINAESPIRALIS MULTIDRUG RESISTANCE GENE (PTR) IN VARIOUS STREPTOMYCES SPP. AND ESCHERICHIA COLI", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 17, no. 5, 1995, pages 1001 - 1012, XP008009342, ISSN: 0950-382X *
SALAH-BEY K ET AL: "UNUSUAL REGULATORY MECHANISM FOR A STREPTOMYCES MULTIDRUG RESISTANCE GENE, PTR, INVOLVING THREE HOMOLOGOUS PROTEIN-BINDING SITES OVERLAPPING THE PROMOTER REGION", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 17, no. 6, 1995, pages 1109 - 1119, XP008009340, ISSN: 0950-382X *

Also Published As

Publication number Publication date
WO2002033104A2 (fr) 2002-04-25
AU2002210802A1 (en) 2002-04-29

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