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WO2002032537A3 - Improved method of expanded bed chromatography - Google Patents

Improved method of expanded bed chromatography Download PDF

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Publication number
WO2002032537A3
WO2002032537A3 PCT/US2001/027736 US0127736W WO0232537A3 WO 2002032537 A3 WO2002032537 A3 WO 2002032537A3 US 0127736 W US0127736 W US 0127736W WO 0232537 A3 WO0232537 A3 WO 0232537A3
Authority
WO
WIPO (PCT)
Prior art keywords
resin
sample solution
improved method
mixture
expanded bed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/027736
Other languages
French (fr)
Other versions
WO2002032537A2 (en
Inventor
Walter Francis Junior Prouty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Priority to AU2001296228A priority Critical patent/AU2001296228A1/en
Publication of WO2002032537A2 publication Critical patent/WO2002032537A2/en
Publication of WO2002032537A3 publication Critical patent/WO2002032537A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1807—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using counter-currents, e.g. fluidised beds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57563—Vasoactive intestinal peptide [VIP]; Related peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/58—Conditioning of the sorbent material or stationary liquid the sorbent moving as a whole

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Vascular Medicine (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention is a method of separating a desired component (e.g., a biomolecule such as a recombinant protein) from a mixture such as an unclarified feedstock. In a first step, the mixture is solubilized in an aqueous urea solution to form a sample solution. In a second step, the sample solution is passed upwards through a bed of resin, thereby binding the desired component to the resin and separating the desired component from the mixture. The sample solution comprises a sufficiently high concentration of urea to reduce fouling of resin when the sample solution is passed through the resin.
PCT/US2001/027736 2000-10-13 2001-09-28 Improved method of expanded bed chromatography Ceased WO2002032537A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001296228A AU2001296228A1 (en) 2000-10-13 2001-09-28 Improved method of expanded bed chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24045500P 2000-10-13 2000-10-13
US60/240,455 2000-10-13

Publications (2)

Publication Number Publication Date
WO2002032537A2 WO2002032537A2 (en) 2002-04-25
WO2002032537A3 true WO2002032537A3 (en) 2002-07-11

Family

ID=22906594

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/027736 Ceased WO2002032537A2 (en) 2000-10-13 2001-09-28 Improved method of expanded bed chromatography

Country Status (2)

Country Link
AU (1) AU2001296228A1 (en)
WO (1) WO2002032537A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010066734A1 (en) * 2008-12-08 2010-06-17 Novo Nordisk A/S Counter current purification of polypeptides
CA3233933A1 (en) * 2017-01-30 2018-08-02 Regeneron Pharmaceuticals, Inc. Compositions and methods for reducing bioburden in chromatography
CN107782829A (en) * 2017-11-01 2018-03-09 上海莱士血液制品股份有限公司 One kind utilizes trishydroxymethylaminomethane in sulfate by ion chromatography biological products（Tris）Method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1042892A (en) * 1996-08-01 1998-02-17 Higeta Shoyu Co Ltd Recovery and purification of protein

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1042892A (en) * 1996-08-01 1998-02-17 Higeta Shoyu Co Ltd Recovery and purification of protein

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIOSEPARATION, vol. 8, no. 1-5, 1999, pages 219 - 228, ISSN: 0923-179X *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1999, BROBJER MICHAEL: "Development and scale up of a capture step (expanded bed chromatography) for a fusion protein expressed intracellularly in Escherichia coli.", XP002194008, Database accession no. PREV200000091626 *
DATABASE WPI Section Ch Week 199817, Derwent World Patents Index; Class B04, AN 1998-131057, XP002194009 *
HJORTH R: "Expanded-bed adsorption in industrial bioprocessing: recent developments", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 15, no. 6, 1 June 1997 (1997-06-01), pages 230 - 235, XP004064512, ISSN: 0167-7799 *
JOHANSSON H J ET AL: "Large scale recovery and purification of periplasmic recombinant protein from E. coli using expanded bed adsorption chromatography followed by new ion exchange media", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 48, no. 1, 18 July 1996 (1996-07-18), pages 9 - 14, XP004037029, ISSN: 0168-1656 *

Also Published As

Publication number Publication date
WO2002032537A2 (en) 2002-04-25
AU2001296228A1 (en) 2002-04-29

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