[go: up one dir, main page]

WO2002002735A1 - Tube de centrifugation ouvert sur le haut et le bas - Google Patents

Tube de centrifugation ouvert sur le haut et le bas Download PDF

Info

Publication number
WO2002002735A1
WO2002002735A1 PCT/KR2001/000659 KR0100659W WO0202735A1 WO 2002002735 A1 WO2002002735 A1 WO 2002002735A1 KR 0100659 W KR0100659 W KR 0100659W WO 0202735 A1 WO0202735 A1 WO 0202735A1
Authority
WO
WIPO (PCT)
Prior art keywords
tube
section
cells
transparent
ring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2001/000659
Other languages
English (en)
Inventor
Soon Wook Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU2001252743A priority Critical patent/AU2001252743A1/en
Publication of WO2002002735A1 publication Critical patent/WO2002002735A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/02—Casings; Lids
    • B04B7/04—Casings facilitating discharge
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones

Definitions

  • the present invention relates to a top and bottom open centrifuge tube for accelerating the separation process of a multitude of cells and bacteria and improving the accuracy of examination and also at the same time allows the simple separation between liquid and foreign substances possible within the premises of examination center or research center. More specifically, the present invention relates to a top and bottom open centrifuge tube for separating a multitude of cells and bacteria during the bacteria cultivation of cerebrospinal fluid, ascitic fluid and pleural fluid, or cancer examination, or bacteria cultivation examination using various types of cotton sticks especially when rotating the centrifugal separation tube in order to precipitate the cells and bacteria at the bottom of the tube and collecting them in a ring-shaped collection tube after dipping a cotton stick into the centrifugal separation tube where a transporting cultivation paper is located and then coating on a slide or cultivating the bacteria.
  • the conventional method of examining bacteria usually involves collecting examining bodies (cells, bacteria) using a cotton stick from the patient's affected part and inserting the cotton stick into a test tube where a transporting cultivation paper is located and transporting the test tube to an examination room.
  • a transporting cultivation paper is located and transporting the test tube to an examination room.
  • an accurate examination work becomes very difficult when stained to jell type cultivation paper or the examination body is left behind in the test tube.
  • cerebrospinal fluid, ascitic fluid and pleural fluid to test the existence of cancer cells or leukemia
  • the remaining liquid after a centrifugal separation was discarded to leave only the precipitates for collecting the cells and the precipitates were then coated on the slide. This process was time consuming and some of the cells were lost in the process.
  • the examination body container used in each of the hospital, pharmaceutical company, food research center and quarantine center has a lid on the top and the bottom part is sealed. As a result, some serious difficulties arise due to the problems as mentioned above during the cell separation.
  • FIG. 1 is a disassembled view of centrifugal cell separation transparent tube according to the present invention.
  • FIG. 2 is a cross section of assembled centrifugal cell separation transparent tube according to the present invention.
  • FIG. 3 shows the pumping tube section on the lid at the upper end of disassembled view of centrifugal cell separation transparent tube according to another embodiment of the present invention.
  • FIG. 4 is a disassembled view of centrifugal cell separation transparent tube according to further embodiment of the present invention.
  • FIG. 5 shows the cross section of a practical example of centrifugal cell separation transparent tube in FIG. 4.
  • FIG. 6 shows the pumping tube section on the lid at the upper end of disassembled view of centrifugal cell separation transparent tube according to another embodiment of FIG.
  • the present invention is designed to overcome the above problems of prior arts.
  • the present invention comprises a conventional lid fixed by a screw on the upper end of a transparent synthetic resin tube, a single body ring-shaped collection tube in a gourd shape on the lower end of the transparent synthetic resin tube and a bolt and a nut section at the tapered section on the upper section of the ring-shaped collection tube.
  • the bolt section is united with a support cap and a watertight cap is formed at the center section of the support cap in order to seal the entry of the ring-type collection tube.
  • the present invention allows a convenient and accurate examination within shorter examination time possible by being able to separate the cells without discarding the liquid at the time of cell separation. This is achieved by uniting with the support cap at the lower section of the transparent tube and inserting the liquid or inserting the collected cells to the liquid while the lid at the upper section open and allowing the cells accumulate within the ring-shaped collection tube through downward precipitation when the centrifugal separation is performed.
  • a tube with an opening/closing lid at the upper end of opening section comprising: a tapered section 5 in a funnel shape at the lower end of a synthetic resin body 2; a transparent tube 1 which forms a single body with a ring-shape collection tube in a gourd shape with an entry at the lower section; a transparent support cap 9, where a bolt section is formed at the connection between the lower end of said transparent tube 1 and the tapered section; a ring-shape collection tube 6 which forms a single body with a water-tight cap 8 that is located at the center of the transparent support cap 9 which in itself unites with the lower end of said transparent tube 1 and is designed to seal the entry 7 by unifying with the transparent tube 1; wherein the cells precipitate in the ring- shape collection tube 6 if the cells 13 or the bodily fluid on which the cells are floating are inserted to the liquid 12 in said transparent tube 1 and the cells can be separated by contracting the body of synthetic resin transparent tube 1 during the cell separation.
  • the upper section of the lid that unites with the upper end opening section of said transparent tube 1 is formed in a concave tube shape in order to be able to pump out during the cell separation.
  • the collected cells 13 from the patient's affected part are inserted to the liquid 12 contained in the transparent tube through the opening section at the upper end after sealing the entry 7 of the ring-shape collection tube 6 at the lower end by uniting with the transparent support cap at the lower end of the transparent tube 1.
  • the cells in the liquid are precipitated downward and collected in the ring-shaped collection tube 6 consequently forming a accumulation as shown in FIG. 2.
  • the cells 13 (examining body) collected in the ring-shaped collection tube 6 when coated on a slide cultivation paper only the cells can be separated and withdrawn if the body 12 of the synthetic resin transparent tube 1 is pressed slightly after opening the transparent support cap 9.
  • the lid that unites with the upper end opening section of said transparent tube 1 is formed in flexible synthetic resin or a tube in rubber material in order to be in a concave tube shape as shown in FIG. 3.
  • the concave tube part at the upper end of the lid 4' can be pumped in order to separate the cells instead of pressing the body 2 for pumping purpose.
  • a bolt section is formed at the connection between the lower end of the transparent tube 1 ' and the tapered section 5', in order to seal the watertight cap 8 and withdrawal tube 7' when united with the transparent support cap.
  • the transparent tube 1 ' in FIG. 4 when the examining body is inserted to the liquid the cells accumulate in the withdrawal tube T at the lower end of the funnel shaped tapered section therefore the cells accumulated in the withdrawal tube 7' can be withdrawn by pumping the lid 4' that has a concave tube at the upper end or at the body of the transparent tube 1'.
  • the present invention is convenient and simple for separating cells, bacteria and liquid but its use is not only limited for examining cancer cells. It can also be used for separating between testing sample and testing medicine using a centrifugal separator in a quarantine office for examining the farming products and livestock.
  • test tube and sampling tube are not necessary instead the present invention can perform these two functions.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Centrifugal Separators (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un tube de séparation centrifuge pouvant être ouvert et fermé sur le haut et le bas, destiné à accélérer le processus de séparation d'une multitude de cellules et de bactéries, et à améliorer la précision d'examen, ledit tube permettant par ailleurs la séparation simple de liquides et de substances étrangères. Le tube selon l'invention est composé d'un couvercle conventionnel fixé par une vis sur l'extrémité supérieure d'un tube en résine transparente synthétique, d'un tube de recueillement annulaire monocorps se présentant sous la forme d'une calebasse, disposé sur l'extrémité inférieure du tube en résine transparente synthétique, ainsi que d'une partie boulon et d'une partie écrou sur la partie effilée au niveau de la partie supérieure du tube de recueillement annulaire. La partie boulon est reliée à un bouchon support, et un bouchon étanche à l'eau est formé au centre du bouchon support de manière à fermer l'entrée du tube de recueillement annulaire. Ladite invention permet d'effectuer des examens de manière aisée et précise sur une durée d'examen réduite, les cellules pouvant être séparées sans suppression du liquide au moment de la séparation cellulaire. A cet effet, on relie le bouchon support au niveau de la partie inférieure avec le tube transparent, on introduit le liquide ou les cellules collectées dans le liquide, le couvercle étant ouvert au niveau de la section supérieure, et on laisse les cellules s'accumuler à l'intérieur du tube de recueillement annulaire par précipitation descendante lors de la séparation centrifuge.
PCT/KR2001/000659 2000-06-21 2001-04-20 Tube de centrifugation ouvert sur le haut et le bas Ceased WO2002002735A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001252743A AU2001252743A1 (en) 2000-06-21 2001-04-20 Top and bottom open centrifuge tube

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR2000/34212 2000-06-21
KR1020000034212A KR100354341B1 (ko) 2000-06-21 2000-06-21 상하 개폐식 원심 분리 튜브

Publications (1)

Publication Number Publication Date
WO2002002735A1 true WO2002002735A1 (fr) 2002-01-10

Family

ID=19673007

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2001/000659 Ceased WO2002002735A1 (fr) 2000-06-21 2001-04-20 Tube de centrifugation ouvert sur le haut et le bas

Country Status (3)

Country Link
KR (1) KR100354341B1 (fr)
AU (1) AU2001252743A1 (fr)
WO (1) WO2002002735A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6866826B2 (en) * 2000-12-30 2005-03-15 Beckman Coulter, Inc. Large mouth centrifuge labware
CN102844121A (zh) * 2009-12-19 2012-12-26 全玟墉 离心分离管
CN103212125A (zh) * 2013-04-01 2013-07-24 浙江康慈医疗科技有限公司 一种快速提取富血小板高浓度血浆的方法及专用设备
CN106442091A (zh) * 2016-10-31 2017-02-22 上海化工研究院 基于热气吹脱法富集含水固体中痕量挥发性有机物的装置
GB2574046A (en) * 2018-05-24 2019-11-27 Oxford Nanopore Tech Ltd Improved container

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100749439B1 (ko) * 2006-07-28 2007-08-14 임성오 원심분리기의 회전통
KR100890316B1 (ko) * 2007-06-25 2009-03-26 한국원자력연구원 원심분리 튜브
WO2011071353A2 (fr) * 2009-12-07 2011-06-16 Jeon Min-Yong Tube pour centrifugeuse
KR101188660B1 (ko) 2010-07-08 2012-10-08 주식회사 무한기업 수위조절이 가능한 원심분리기용 시험관
CN103543057B (zh) * 2013-11-13 2015-12-02 何鑫 用于微量脱落细胞制备细胞学蜡块用的组合式离心管
KR101594539B1 (ko) * 2014-02-17 2016-02-16 한국기초과학지원연구원 세포 응집용 농축 시험관
CN106140494A (zh) * 2015-04-02 2016-11-23 马铁军 一种离心时使用的特殊底开口离心管装置
CN104990780B (zh) * 2015-07-26 2017-10-17 赵稳兴 一种制作细胞块的方法
KR101848869B1 (ko) 2016-08-19 2018-04-13 (주) 굿모닝 바이오 원심분리용 용기
KR102274063B1 (ko) * 2021-02-15 2021-07-08 최성민 시료의 전처리용 캐쳐스 정제 키트 및 이 정제 키트를 이용한 시료의 전처리 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4364903A (en) * 1981-04-02 1982-12-21 Becton, Dickinson And Company Contamination-free separation device
KR880004313A (ko) * 1986-12-24 1988-06-03 전동명 경사시키지 않는 뇨 튜브장치 및 분리방법
JPH1164183A (ja) * 1997-08-07 1999-03-05 Bokei Cho 尿沈渣を自動的に保持する尿分析用の定量遠心分離チューブ
US5996811A (en) * 1997-02-13 1999-12-07 Fuji Photo Film Co. Ltd. Plasma collecting device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4364903A (en) * 1981-04-02 1982-12-21 Becton, Dickinson And Company Contamination-free separation device
KR880004313A (ko) * 1986-12-24 1988-06-03 전동명 경사시키지 않는 뇨 튜브장치 및 분리방법
US5996811A (en) * 1997-02-13 1999-12-07 Fuji Photo Film Co. Ltd. Plasma collecting device
JPH1164183A (ja) * 1997-08-07 1999-03-05 Bokei Cho 尿沈渣を自動的に保持する尿分析用の定量遠心分離チューブ

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6866826B2 (en) * 2000-12-30 2005-03-15 Beckman Coulter, Inc. Large mouth centrifuge labware
CN102844121A (zh) * 2009-12-19 2012-12-26 全玟墉 离心分离管
CN103212125A (zh) * 2013-04-01 2013-07-24 浙江康慈医疗科技有限公司 一种快速提取富血小板高浓度血浆的方法及专用设备
CN106442091A (zh) * 2016-10-31 2017-02-22 上海化工研究院 基于热气吹脱法富集含水固体中痕量挥发性有机物的装置
GB2574046A (en) * 2018-05-24 2019-11-27 Oxford Nanopore Tech Ltd Improved container
GB2574046B (en) * 2018-05-24 2022-12-07 Oxford Nanopore Tech Plc Improved Container

Also Published As

Publication number Publication date
KR100354341B1 (ko) 2002-09-30
KR20010114105A (ko) 2001-12-29
AU2001252743A1 (en) 2002-01-14

Similar Documents

Publication Publication Date Title
WO2002002735A1 (fr) Tube de centrifugation ouvert sur le haut et le bas
EP1284160B1 (fr) Dispositif pour le prélèvement d'échantillons liquides
US7153477B2 (en) Device and method for separating components of a fluid sample
US4465200A (en) Low contamination closure for blood collection tubes
US3957653A (en) Apparatus for collection, separation and isolation of blood
EP0535212B1 (fr) Recipient a enchantillons de liquide et ses modules de test
US4973450A (en) Device for urinalysis
EP1014088A2 (fr) Appareil et procédé pour séparer des constituants d'un échantillon liquide
CN108871908A (zh) 生物样本处理装置
CA2084778A1 (fr) Methode et appareil pour cytologie et epreuves microbiologiques
US6582665B2 (en) Universal collection and transfer system
EP1106065A2 (fr) Procédé et récipient pour conserver et expédier du tissu, du fluide, ou de la matière cellulaire vivante
US20040025603A1 (en) Test tube insert
US4771486A (en) Sputum specimen collecting device
EP0189153A2 (fr) Dispositif de prise de sang
CN208833572U (zh) 生物样本处理装置
US6106483A (en) Apparatus for obtaining a cytology monolayer
US12296331B2 (en) Fluid collection device
US5952239A (en) Cytology chamber with port to receive collection bottle and method of use
EP1254718A2 (fr) Tube sous vide et procédé pour examen microscopique de sédiment d'urine, analyses chimiques et microbiologiques
CN105527125B (zh) 一种流体收集器
CN210037309U (zh) 一种样品采集装置及用于对血液样品进行检测的试剂盒
SU1502035A1 (ru) Устройство дл сбора аспирата
CN209264315U (zh) 样本取样隔离取样检测装置
KR200205096Y1 (ko) 상하 개폐식 원심분리 튜브

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AU CA CN JP RU US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP