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WO2002000847A3 - Pluripotent embryonic stem (es) cell lines, improved methods for their production, and their use for germ line transmission and for the generation of genetically modified animals - Google Patents

Pluripotent embryonic stem (es) cell lines, improved methods for their production, and their use for germ line transmission and for the generation of genetically modified animals Download PDF

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Publication number
WO2002000847A3
WO2002000847A3 PCT/EP2001/007396 EP0107396W WO0200847A3 WO 2002000847 A3 WO2002000847 A3 WO 2002000847A3 EP 0107396 W EP0107396 W EP 0107396W WO 0200847 A3 WO0200847 A3 WO 0200847A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell lines
embryonic stem
production
germ line
generation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/007396
Other languages
French (fr)
Other versions
WO2002000847A2 (en
Inventor
Luc Schoonjans
Randall Moreadith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thromb X NV
Original Assignee
Thromb X NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thromb X NV filed Critical Thromb X NV
Priority to AU2001289615A priority Critical patent/AU2001289615A1/en
Priority to EP01969324A priority patent/EP1305401A2/en
Priority to US10/312,202 priority patent/US20040053406A1/en
Publication of WO2002000847A2 publication Critical patent/WO2002000847A2/en
Publication of WO2002000847A3 publication Critical patent/WO2002000847A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a novel composition for maintaining and growing pluripotent and germ line competent mouse embryonic stem cells. The composition includes high glucose DMEM, non-essential amino acids, glutamine, beta-mercaptoethanol and fetal bovine serum or the equivalents thereof, which is conditioned by an immortalized rabbit fibroblast cell line transduced with genomic rabbit Leukemia Inhibitory Factor (LIF). The invention further relates to the use of the composition for producing embryonic stem cell lines and to the use of these cell lines in the production of transgenic animals.
PCT/EP2001/007396 2000-06-28 2001-06-28 Pluripotent embryonic stem (es) cell lines, improved methods for their production, and their use for germ line transmission and for the generation of genetically modified animals Ceased WO2002000847A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2001289615A AU2001289615A1 (en) 2000-06-28 2001-06-28 Pluripotent embryonic stem (es) cell lines, improved methods for their production, and their use for germ line transmission and for the generation of genetically modified animals
EP01969324A EP1305401A2 (en) 2000-06-28 2001-06-28 Pluripotent embryonic stem (es) cell lines, improved methods for their production, and their use for germ line transmission and for the generation of genetically modified animals
US10/312,202 US20040053406A1 (en) 2000-06-28 2001-06-28 Pluripotent embryonic stem (es) cell lines, improved methods for their production, and their use for germ line transmission and for the generation of genetically modified animals

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP00202254 2000-06-28
EP00202254.9 2000-06-28
US62888300A 2000-07-31 2000-07-31
US09/628,883 2000-07-31

Publications (2)

Publication Number Publication Date
WO2002000847A2 WO2002000847A2 (en) 2002-01-03
WO2002000847A3 true WO2002000847A3 (en) 2002-07-18

Family

ID=26072427

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/007396 Ceased WO2002000847A2 (en) 2000-06-28 2001-06-28 Pluripotent embryonic stem (es) cell lines, improved methods for their production, and their use for germ line transmission and for the generation of genetically modified animals

Country Status (3)

Country Link
EP (1) EP1305401A2 (en)
AU (1) AU2001289615A1 (en)
WO (1) WO2002000847A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4374419B2 (en) 2002-10-31 2009-12-02 独立行政法人理化学研究所 Composition for pluripotent stem cell culture and use thereof
PL2964229T3 (en) 2013-03-06 2020-05-18 Janssen Pharmaceutica Nv Benzoimidazol-2-yl pyrimidine modulators of the histamine h4 receptor

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602307A (en) * 1992-08-12 1997-02-11 Baylor College Of Medicine Non-human animal having predefined allele of a cellular adhesion gene
US5690926A (en) * 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
JPH1056915A (en) * 1996-06-13 1998-03-03 Takeda Chem Ind Ltd Animal having inactivated ldl receptor gene
WO1999009141A1 (en) * 1997-08-14 1999-02-25 Biotransplant, Inc. Porcine totipotent cells and method for long-term culture

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602307A (en) * 1992-08-12 1997-02-11 Baylor College Of Medicine Non-human animal having predefined allele of a cellular adhesion gene
US5690926A (en) * 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
JPH1056915A (en) * 1996-06-13 1998-03-03 Takeda Chem Ind Ltd Animal having inactivated ldl receptor gene
WO1999009141A1 (en) * 1997-08-14 1999-02-25 Biotransplant, Inc. Porcine totipotent cells and method for long-term culture

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Derwent World Patents Index; AN 1998-210272, XP002189758, TAKEDA CHEM IND LTD: "Transgenic animals having inactivated LDL receptor gene" *
MOREADITH R W ET AL: "GENE TARGETING IN EMBRYONIC STEM CELLS: THE NEW PHYSIOLOGY AND METABOLISM", JOURNAL OF MOLECULAR MEDICINE, SPRINGER VERLAG, DE, vol. 75, 1997, pages 208 - 216, XP002917335, ISSN: 0946-2716 *
RAMIREZ-SOLIS R ET AL: "GENE TARGETING IN EMBRYONIC STEM CELLS", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 225, 1992, pages 855 - 878, XP000918540, ISSN: 0076-6879 *
SLAGER H G ET AL: "SECRETION OF TRANSFORMING GROWTH FACTOR-BETA ISOFORMS BY EMBRYONIC STEM CELLS: ISOFORM AND LATENCY ARE DEPENDENT ON DIRECTION OF DIFFERENTIATION", JOURNAL OF CELLULAR PHYSIOLOGY, NEW YORK,NY, US, vol. 156, no. 2, 1 August 1993 (1993-08-01), pages 247 - 256, XP000644712, ISSN: 0021-9541 *
THOMPSON S ET AL: "GERM LINE TRANSMISSION AND EXPRESSION OF A CORRECTED HPRT GENE PRODUCED BY GENE TARGETING IN EMBRYONIC STEM CELLS", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 56, no. 2, 27 January 1989 (1989-01-27), pages 313 - 321, XP001061551, ISSN: 0092-8674 *
ZIJLSTRA ET AL: "Germ-line transmission of a disrupted beta2-microglobulin gene produced by homologous recombination in embryonic cells", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 342, no. 248, 23 November 1989 (1989-11-23), pages 435 - 438, XP002113106, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
WO2002000847A2 (en) 2002-01-03
EP1305401A2 (en) 2003-05-02
AU2001289615A1 (en) 2002-01-08

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