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WO2002090598A3 - Methods for the identification of inhibitors of flavanone 3-hydroxylase expression or activity in plants - Google Patents

Methods for the identification of inhibitors of flavanone 3-hydroxylase expression or activity in plants Download PDF

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Publication number
WO2002090598A3
WO2002090598A3 PCT/US2002/014694 US0214694W WO02090598A3 WO 2002090598 A3 WO2002090598 A3 WO 2002090598A3 US 0214694 W US0214694 W US 0214694W WO 02090598 A3 WO02090598 A3 WO 02090598A3
Authority
WO
WIPO (PCT)
Prior art keywords
fht
activity
identification
flavanone
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/014694
Other languages
French (fr)
Other versions
WO2002090598A2 (en
Inventor
Keith Davis
Adel Zayed
Robert Ascenzi
Douglas Boyes
Neil Hoffman
Jorn Gorlach
Jeffrey Woessner
Carol Hamilton
Kenneth Phillips
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cogenics Icoria Inc
Original Assignee
Paradigm Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc filed Critical Paradigm Genetics Inc
Priority to EP02726854A priority Critical patent/EP1419269A2/en
Publication of WO2002090598A2 publication Critical patent/WO2002090598A2/en
Anticipated expiration legal-status Critical
Publication of WO2002090598A3 publication Critical patent/WO2002090598A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present inventors have discovered that Flavanone 3-hydroxylase ('FHT') is essential for plant growth. Specifically, the inhibition of FHT gene expression in plant seedling results in small and chlorotic seedlings. Thus, FHT can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit FHT expression or activity. The present invention can be used to identify compounds having herbicide activity by contacting a compound with FHT and detecting the presence and/or absence of binding between the compound and the FHT, or alternatively, detecting a decrease in FHT expression or activity.
PCT/US2002/014694 2001-05-08 2002-05-08 Methods for the identification of inhibitors of flavanone 3-hydroxylase expression or activity in plants Ceased WO2002090598A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02726854A EP1419269A2 (en) 2001-05-08 2002-05-08 Methods for the identification of inhibitors of flavanone 3-hydroxylase expression or activity in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28949201P 2001-05-08 2001-05-08
US60/289,492 2001-05-08

Publications (2)

Publication Number Publication Date
WO2002090598A2 WO2002090598A2 (en) 2002-11-14
WO2002090598A3 true WO2002090598A3 (en) 2004-03-25

Family

ID=23111769

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/014694 Ceased WO2002090598A2 (en) 2001-05-08 2002-05-08 Methods for the identification of inhibitors of flavanone 3-hydroxylase expression or activity in plants

Country Status (2)

Country Link
EP (1) EP1419269A2 (en)
WO (1) WO2002090598A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5349125A (en) * 1991-07-11 1994-09-20 International Flower Developments Pty. Ltd. Genetic sequences encoding a 3',5'-hydroxylase and uses therefor
US5733759A (en) * 1992-03-09 1998-03-31 Washington State University Research Foundation Methods for the regulation of plant fertility
US5859329A (en) * 1992-08-05 1999-01-12 International Flower Developments Pty. Ltd. Genetic sequences encoding flavonol synthase enzymes and uses therefor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5349125A (en) * 1991-07-11 1994-09-20 International Flower Developments Pty. Ltd. Genetic sequences encoding a 3',5'-hydroxylase and uses therefor
US5733759A (en) * 1992-03-09 1998-03-31 Washington State University Research Foundation Methods for the regulation of plant fertility
US5859329A (en) * 1992-08-05 1999-01-12 International Flower Developments Pty. Ltd. Genetic sequences encoding flavonol synthase enzymes and uses therefor

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BRITSCH ET AL.: "Purification and characterization of (2S)-flavanone 3-hydroxylase from petunia hybrida", EUROPENA JOURNAL OF BIOCHEMISTRY, vol. 156, no. 3, May 1986 (1986-05-01), pages 569, XP002952731 *
LUKACIN ET AL.: "Site-directed mutagenesis of the active site serine290 in flavanone 3beta-hydroxylase from petunia hybrida", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 267, 2000, pages 853 - 860, XP002952728 *
WINKEL-SHIRLEY B.: "It takes a garden. How work on diverse plant species has contributed to an understanding of flavonoid metabolism", PLANT PHYSIOLOGY, vol. 127, December 2001 (2001-12-01), pages 1399 - 1404, XP002952729 *

Also Published As

Publication number Publication date
EP1419269A2 (en) 2004-05-19
WO2002090598A2 (en) 2002-11-14

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