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WO2002068459A3 - Proteines fluorescentes anti-agregantes et leurs methodes d'utilisation - Google Patents

Proteines fluorescentes anti-agregantes et leurs methodes d'utilisation Download PDF

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Publication number
WO2002068459A3
WO2002068459A3 PCT/US2002/005749 US0205749W WO02068459A3 WO 2002068459 A3 WO2002068459 A3 WO 2002068459A3 US 0205749 W US0205749 W US 0205749W WO 02068459 A3 WO02068459 A3 WO 02068459A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
subject
protein
nucleic acid
aggregating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/005749
Other languages
English (en)
Other versions
WO2002068459A2 (fr
Inventor
Sergey Lukyanov
Konstantin Lukyanov
Yuriy Yanushevich
Alexandr Savistky
Arcady Fradkov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Bio USA Inc
Original Assignee
Clontech Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/006,922 external-priority patent/US7166444B2/en
Application filed by Clontech Laboratories Inc filed Critical Clontech Laboratories Inc
Priority to EP02723238A priority Critical patent/EP1385967A2/fr
Priority to JP2002567969A priority patent/JP2004536571A/ja
Priority to CA2434737A priority patent/CA2434737C/fr
Priority to AU2002254031A priority patent/AU2002254031A1/en
Publication of WO2002068459A2 publication Critical patent/WO2002068459A2/fr
Anticipated expiration legal-status Critical
Publication of WO2002068459A3 publication Critical patent/WO2002068459A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne des compositions à base d'acides nucléiques codant des chromo/fluoroprotéines anti-agrégantes et des gènes mutants associés, ainsi que des protéines codées par ceux-ci. Ces protéines sont des polypeptides composés de protéines anti-agrégantes colorées et/ou fluorescentes, dans lesquelles la caractéristique d'anti-agrégation résulte de la modulation des résidus N-terminal de la protéine, et la caractéristique de coloration et/ou de fluorescence découle de l'interaction entre deux résidus ou plus de la protéine. Par ailleurs, l'invention concerne des fragments des acides nucléiques précités et des peptides codés par ceux-ci, ainsi que des anticorps dirigées contre les protéines, cellules et organismes transgéniques en question. On peut utiliser les compositions à base de protéines et d'acides nucléiques précitées dans diverses applications différentes. L'invention concerne enfin des kits destinés à ces applications, contenant par exemple les compositions d'acides nucléiques susmentionnées.
PCT/US2002/005749 2001-02-21 2002-02-20 Proteines fluorescentes anti-agregantes et leurs methodes d'utilisation Ceased WO2002068459A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP02723238A EP1385967A2 (fr) 2001-02-21 2002-02-20 Proteines fluoresantes anti-aggregantes et leurs methodes d'utilisation
JP2002567969A JP2004536571A (ja) 2001-02-21 2002-02-20 非凝集性蛍光性タンパク質およびその使用方法
CA2434737A CA2434737C (fr) 2001-02-21 2002-02-20 Proteines fluorescentes anti-agregantes et leurs methodes d'utilisation
AU2002254031A AU2002254031A1 (en) 2001-02-21 2002-02-20 Non aggregating fluorescent proteins and methods for using the same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US27098301P 2001-02-21 2001-02-21
US60/270,983 2001-02-21
US10/006,922 US7166444B2 (en) 1999-10-14 2001-12-04 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US10/006,922 2001-12-04

Publications (2)

Publication Number Publication Date
WO2002068459A2 WO2002068459A2 (fr) 2002-09-06
WO2002068459A3 true WO2002068459A3 (fr) 2003-11-27

Family

ID=40863845

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/005749 Ceased WO2002068459A2 (fr) 2001-02-21 2002-02-20 Proteines fluorescentes anti-agregantes et leurs methodes d'utilisation

Country Status (5)

Country Link
EP (1) EP1385967A2 (fr)
JP (2) JP2004536571A (fr)
AU (1) AU2002254031A1 (fr)
CA (1) CA2434737C (fr)
WO (1) WO2002068459A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7005511B2 (en) * 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
WO2002068605A2 (fr) * 2001-02-26 2002-09-06 The Regents Of The University Of California Proteines fluorescentes tandem non oligomerisantes
WO2003054158A2 (fr) 2001-12-19 2003-07-03 The University Of Chicago Proteines fluorescentes a maturation rapide et leurs methodes d'utilisation
RU2004118848A (ru) * 2001-12-26 2005-04-20 Клонтек Лэборетериз, Инк. (Us) Мутантные хромофоры/флуорофоры и способы их продуцирования и их применение
CA2511966A1 (fr) * 2002-11-01 2004-07-22 Amgen Inc. Modulateurs de recepteurs de l'hormone parathyroide et de la proteine liee a l'hormone parathyroide
DE602004023199D1 (de) 2003-06-16 2009-10-29 Riken Wako Fluoreszenzprotein und pigmentprotein
US7250298B2 (en) 2004-04-07 2007-07-31 The University Of Chicago Monomeric red fluorescent proteins
EP1994149B1 (fr) 2006-01-25 2010-09-29 Evrogen IP Nouvelles protéines fluorescentes et procédés d'utilisation de celles-ci
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
AU2007314614A1 (en) * 2006-04-14 2008-05-08 Celsense, Inc. Methods for assessing cell labeling
JP7014437B2 (ja) * 2017-01-27 2022-02-01 国立大学法人大阪大学 生体物質の検出方法、それに用いる化学発光指示薬

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001027150A2 (fr) * 1999-10-14 2001-04-19 Clontech Laboratories Inc. Chromophores/fluorophores issus d'anthozoaires et methodes d'utilisation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001027150A2 (fr) * 1999-10-14 2001-04-19 Clontech Laboratories Inc. Chromophores/fluorophores issus d'anthozoaires et methodes d'utilisation

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BAIRD GEOFFREY S ET AL: "Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 97, no. 22, 24 October 2000 (2000-10-24), October 24, 2000, pages 11984 - 11989, XP002208898, ISSN: 0027-8424 *
FRADKOV ARKADY F ET AL: "Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence.", FEBS LETTERS, vol. 479, no. 3, 2000, pages 127 - 130, XP002208900, ISSN: 0014-5793 *
MATZ MIKHAIL V ET AL: "Fluorescent proteins from nonbioluminescent Anthozoa species.", NATURE BIOTECHNOLOGY, vol. 17, no. 10, October 1999 (1999-10-01), pages 969 - 973, XP002208899, ISSN: 1087-0156 *
WALL MARK A ET AL: "The structural basis for red fluorescence in the tetrameric GFP homolog DsRed.", NATURE STRUCTURAL BIOLOGY, vol. 7, no. 12, December 2000 (2000-12-01), pages 1133 - 1138, XP001095366, ISSN: 1072-8368 *
WETZEL RONALD: "Mutations and off-pathway aggregation of proteins.", TRENDS IN BIOTECHNOLOGY, vol. 12, no. 5, 1994, pages 193 - 198, XP001093961, ISSN: 0167-9430 *
WIEHLER JENS ET AL: "Mutants of Discosoma red fluorescent protein with a GFP-like chromophore.", FEBS LETTERS, vol. 487, no. 3, 2001, pages 384 - 389, XP002208901, ISSN: 0014-5793 *
YANUSHEVICH YURII G ET AL: "A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins.", FEBS LETTERS, vol. 511, no. 1-3, 30 January 2002 (2002-01-30), 30 January, 2002, pages 11 - 14, XP002208902, ISSN: 0014-5793 *

Also Published As

Publication number Publication date
EP1385967A2 (fr) 2004-02-04
JP2004536571A (ja) 2004-12-09
CA2434737A1 (fr) 2002-09-06
WO2002068459A2 (fr) 2002-09-06
AU2002254031A1 (en) 2002-09-12
JP2009131260A (ja) 2009-06-18
JP5221308B2 (ja) 2013-06-26
CA2434737C (fr) 2012-11-06

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