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WO2002063039A3 - Gene silencing gene - Google Patents

Gene silencing gene Download PDF

Info

Publication number
WO2002063039A3
WO2002063039A3 PCT/GB2002/000490 GB0200490W WO02063039A3 WO 2002063039 A3 WO2002063039 A3 WO 2002063039A3 GB 0200490 W GB0200490 W GB 0200490W WO 02063039 A3 WO02063039 A3 WO 02063039A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
sde3
polypeptide
ptgs
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2002/000490
Other languages
French (fr)
Other versions
WO2002063039A2 (en
Inventor
David Charles Baulcombe
Tamas Dalmay
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Plant Bioscience Ltd
Original Assignee
Plant Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Priority to AU2002228230A priority Critical patent/AU2002228230A1/en
Publication of WO2002063039A2 publication Critical patent/WO2002063039A2/en
Publication of WO2002063039A3 publication Critical patent/WO2002063039A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Provided are isolated nucleic acid molecules which comprise an sde3 nucleotide sequence which encodes an SDE3 polypeptide capable of mediating Post Transcriptional Gene Silencing (PTGS) of a transgene in a plant into which the nucleic acid is introduced. The SDE3 polypeptide is believed to encode an RNA helicase-like polypeptide. The invention has utility in both enhancing and suppressing the PTGS of transgenes in plants, and methods and materials are provided to achieve this.
PCT/GB2002/000490 2001-02-06 2002-02-01 Gene silencing gene Ceased WO2002063039A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002228230A AU2002228230A1 (en) 2001-02-06 2002-02-01 Gene silencing gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0102936.2 2001-02-06
GB0102936A GB0102936D0 (en) 2001-02-06 2001-02-06 Gene Silencing Gene

Publications (2)

Publication Number Publication Date
WO2002063039A2 WO2002063039A2 (en) 2002-08-15
WO2002063039A3 true WO2002063039A3 (en) 2002-10-03

Family

ID=9908215

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/000490 Ceased WO2002063039A2 (en) 2001-02-06 2002-02-01 Gene silencing gene

Country Status (3)

Country Link
AU (1) AU2002228230A1 (en)
GB (1) GB0102936D0 (en)
WO (1) WO2002063039A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2421534C (en) * 2002-06-28 2004-08-10 Batco Manufacturing Ltd. Particulate material handling systems
US9222100B2 (en) * 2010-08-24 2015-12-29 Monsanto Technology Llc Methods and DNA constructs for autoregulating transgene silencing

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DALMAY T. ET AL: "An RNA-dependent RNA polymerase gene in Arabidopsisis required for posttranscriptional gene silencing mediated by a transgene but not by a virus", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 101, 26 May 2000 (2000-05-26), pages 543 - 553, XP002149067, ISSN: 0092-8674 *
DALMAY TAMAS ET AL: "SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 20, no. 8, 17 April 2001 (2001-04-17), pages 2069 - 2077, XP002207848, ISSN: 0261-4189 *
DATABASE EMBL [online] EBI; 1 October 2000 (2000-10-01), JOHNSON-HOPSON C. ET AL.: "T25N20.11.", XP002207850, Database accession no. Q9LR48 *
DATABASE EMBL [online] EBI; 18 June 1998 (1998-06-18), JOHNSON-HOPSON C. ET AL.: "Genomic sequence for Arabidopsis thaliana BAC T25N20 from chromosome I, complete sequence.", XP002207849, Database accession no. AC005106 *
MOURRAIN P ET AL: "Arabidopsis SGS2 and SGS3 Genes are Required for Posttranscriptional Gene Silencing and Natural Virus Resistance", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 101, 26 May 2000 (2000-05-26), pages 533 - 542, XP002149066, ISSN: 0092-8674 *

Also Published As

Publication number Publication date
GB0102936D0 (en) 2001-03-21
WO2002063039A2 (en) 2002-08-15
AU2002228230A1 (en) 2002-08-19

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