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WO2002059299A3 - Expression cloning method - Google Patents

Expression cloning method Download PDF

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Publication number
WO2002059299A3
WO2002059299A3 PCT/US2002/002392 US0202392W WO02059299A3 WO 2002059299 A3 WO2002059299 A3 WO 2002059299A3 US 0202392 W US0202392 W US 0202392W WO 02059299 A3 WO02059299 A3 WO 02059299A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression cloning
cloning method
proteins involved
cells
glut4 trafficking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/002392
Other languages
French (fr)
Other versions
WO2002059299A2 (en
WO2002059299A9 (en
Inventor
Jonathan S Bogan
Harvey F Lodish
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Whitehead Institute for Biomedical Research
Original Assignee
General Hospital Corp
Whitehead Institute for Biomedical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Hospital Corp, Whitehead Institute for Biomedical Research filed Critical General Hospital Corp
Publication of WO2002059299A2 publication Critical patent/WO2002059299A2/en
Anticipated expiration legal-status Critical
Publication of WO2002059299A3 publication Critical patent/WO2002059299A3/en
Publication of WO2002059299A9 publication Critical patent/WO2002059299A9/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/62—Insulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Described herein is a method of expression cloning useful for identifying and obtaining proteins involved in GLUT4 trafficking in mammalian cells and, thus, in insulin-stimulated glucose uptake by such cells. In particular, an enrichment strategy for expression cloning proteins involved in GLUT4 trafficking at the plasma membrane is described. Proteins identified by the method and uses therefore are also described.
PCT/US2002/002392 2001-01-26 2002-01-28 Expression cloning method Ceased WO2002059299A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US26481601P 2001-01-26 2001-01-26
US60/264,816 2001-01-26
US29896301P 2001-06-18 2001-06-18
US60/298,963 2001-06-18
US32565101P 2001-09-28 2001-09-28
US60/325,651 2001-09-28

Publications (3)

Publication Number Publication Date
WO2002059299A2 WO2002059299A2 (en) 2002-08-01
WO2002059299A3 true WO2002059299A3 (en) 2004-02-12
WO2002059299A9 WO2002059299A9 (en) 2004-08-12

Family

ID=27401738

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/002392 Ceased WO2002059299A2 (en) 2001-01-26 2002-01-28 Expression cloning method

Country Status (1)

Country Link
WO (1) WO2002059299A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9475049B2 (en) 2009-07-31 2016-10-25 Invisible Sentinel, Inc. Analyte detection devices, multiplex and tabletop devices for detection of analyte, and uses thereof
US8012770B2 (en) 2009-07-31 2011-09-06 Invisible Sentinel, Inc. Device for detection of antigens and uses thereof
US9557330B2 (en) 2009-10-09 2017-01-31 Invisible Sentinel, Inc. Device for detection of analytes and uses thereof
US9347938B2 (en) 2012-03-09 2016-05-24 Invisible Sentinel, Inc. Methods for detecting multiple analytes with a single signal

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075188A1 (en) * 1999-06-09 2000-12-14 Whitehead Institute For Biomedical Research Method of measuring plasma membrane targeting of glut4

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075188A1 (en) * 1999-06-09 2000-12-14 Whitehead Institute For Biomedical Research Method of measuring plasma membrane targeting of glut4

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BARTILSON MAGDALENA ET AL: "Differential fluorescence induction reveals Streptococcus pneumoniae loci regulated by competence stimulatory peptide.", MOLECULAR MICROBIOLOGY, vol. 39, no. 1, January 2001 (2001-01-01), pages 126 - 135, XP002225897, ISSN: 0950-382X *
BECKER KARIN ET AL: "Interaction of Fas(Apo-1/CD95) with proteins implicated in the ubiquitination pathway.", FEBS LETTERS, vol. 412, no. 1, 1997, pages 102 - 106, XP002225899, ISSN: 0014-5793 *
BOGAN JONATHAN S ET AL: "Insulin-responsive compartments containing GLUT4 in 3T3-L1 and CHO cells: Regulation by amino acid concentrations.", MOLECULAR AND CELLULAR BIOLOGY, vol. 21, no. 14, July 2001 (2001-07-01), pages 4785 - 4806, XP002225902, ISSN: 0270-7306 *
CARIM-TODD L ET AL: "Identification and characterization of UBXD1, a novel UBX domain-containing gene on human chromosome 19p13, and its mouse ortholog", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1517, no. 2, 26 January 2001 (2001-01-26), pages 298 - 301, XP004248816, ISSN: 0167-4781 *
FOSTER L J ET AL: "A functional role for VAP-33 in insulin-stimulated GLUT4 traffic.", TRAFFIC (COPENHAGEN, DENMARK) DENMARK JUN 2000, vol. 1, no. 6, June 2000 (2000-06-01), pages 512 - 521, XP002225900, ISSN: 1398-9219 *
LADANYI MARC ET AL: "The der(17)t(X;17)(p11;q25) of human alveolar soft part sarcoma fuses the TFE3 transcription factor gene to ASPL, a novel gene at 17q25.", ONCOGENE, vol. 20, no. 1, 2001, pages 48 - 57, XP009003328, ISSN: 0950-9232 *
NAKAYAMA JUN ET AL: "Expression cloning of a human polysialyltransferase that forms the polysialylated neural cell adhesion molecular present in embryonic brain.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 92, no. 15, 1995, 1995, pages 7031 - 7035, XP002225896, ISSN: 0027-8424 *
ORITANI K ET AL: "Identification of stromal cell products that interact with pre-B cells.", THE JOURNAL OF CELL BIOLOGY. UNITED STATES AUG 1996, vol. 134, no. 3, August 1996 (1996-08-01), pages 771 - 782, XP009001265, ISSN: 0021-9525 *
PATKI VARSHA ET AL: "Insulin action on GLUT4 traffic visualized in single 3T3-L1 adipocytes by using ultra-fast microscopy.", MOLECULAR BIOLOGY OF THE CELL, vol. 12, no. 1, January 2001 (2001-01-01), pages 129 - 141, XP002225901, ISSN: 1059-1524 *
SASAKI ET AL: "Expression cloning of a GM3-specific alpha-2,8-sialyltransferase (GD3 synthase)", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 269, no. 22, 3 June 1994 (1994-06-03), pages 15950 - 15956, XP002084563, ISSN: 0021-9258 *
SIMPSON F ET AL: "GLUT4--at the cross roads between membrane trafficking and signal transduction.", TRAFFIC (COPENHAGEN, DENMARK) DENMARK JAN 2001, vol. 2, no. 1, January 2001 (2001-01-01), pages 2 - 11, XP002225903, ISSN: 1398-9219 *
VOLLENWEIDER PETER ET AL: "The small guanosine triphosphate-binding protein Rab4 is involved in insulin-induced GLUT4 translocation and actin filament rearrangement in 3T3-L1 cells.", ENDOCRINOLOGY, vol. 138, no. 11, 1997, pages 4941 - 4949, XP002225898, ISSN: 0013-7227 *

Also Published As

Publication number Publication date
WO2002059299A2 (en) 2002-08-01
WO2002059299A9 (en) 2004-08-12

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