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WO2002056004A3 - Appareil et procede d'electrophorese a film mince - Google Patents

Appareil et procede d'electrophorese a film mince Download PDF

Info

Publication number
WO2002056004A3
WO2002056004A3 PCT/US2002/000985 US0200985W WO02056004A3 WO 2002056004 A3 WO2002056004 A3 WO 2002056004A3 US 0200985 W US0200985 W US 0200985W WO 02056004 A3 WO02056004 A3 WO 02056004A3
Authority
WO
WIPO (PCT)
Prior art keywords
data
thin film
electrophoresis apparatus
electrophoresis
film electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/000985
Other languages
English (en)
Other versions
WO2002056004A8 (fr
WO2002056004A2 (fr
Inventor
Andras Guttman
Bart Wanders
Phil Alei
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Participations AG
Original Assignee
Syngenta Participations AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations AG filed Critical Syngenta Participations AG
Priority to CA002434469A priority Critical patent/CA2434469A1/fr
Priority to EP02705777A priority patent/EP1412733A2/fr
Priority claimed from US10/047,461 external-priority patent/US20030132117A1/en
Publication of WO2002056004A2 publication Critical patent/WO2002056004A2/fr
Publication of WO2002056004A8 publication Critical patent/WO2002056004A8/fr
Anticipated expiration legal-status Critical
Publication of WO2002056004A3 publication Critical patent/WO2002056004A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Electrochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une électrophorèse à débit élevé dans laquelle une pluralité d'échantillons migrent à travers un milieu de séparation de très grande résistivité sous l'effet d'un champ électrique. De multiples passages d'électrophorèse sont exécutés au moyen d'un seul milieu de séparation. En utilisant un densitomètre optique ou d'autres systèmes de détection, des techniques automatiques d'analyse de données permettent d'obtenir des données quantitatives et qualitatives. Les températures de fonctionnement régulées et la géométrie réduite permettent d'atténuer l'imprécision de dispersion dans les données en bandes.
PCT/US2002/000985 2001-01-12 2002-01-14 Appareil et procede d'electrophorese a film mince Ceased WO2002056004A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002434469A CA2434469A1 (fr) 2001-01-12 2002-01-14 Appareil et procede d'electrophorese a film mince
EP02705777A EP1412733A2 (fr) 2001-01-12 2002-01-14 Appareil et procede d'electrophorese a film mince

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US75998901A 2001-01-12 2001-01-12
US09/759,989 2001-01-12
US10/047,461 2002-01-14
US10/047,461 US20030132117A1 (en) 2002-01-14 2002-01-14 Thin film electrophoresis apparatus and method

Publications (3)

Publication Number Publication Date
WO2002056004A2 WO2002056004A2 (fr) 2002-07-18
WO2002056004A8 WO2002056004A8 (fr) 2003-01-16
WO2002056004A3 true WO2002056004A3 (fr) 2004-02-19

Family

ID=26725040

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2002/000985 Ceased WO2002056004A2 (fr) 2001-01-12 2002-01-14 Appareil et procede d'electrophorese a film mince
PCT/US2002/000993 Ceased WO2002055189A2 (fr) 2001-01-12 2002-01-14 Chambre de reaction de membrane nanoporeuse pour reactions miniaturisees et reactions cinetiques ameliorees

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2002/000993 Ceased WO2002055189A2 (fr) 2001-01-12 2002-01-14 Chambre de reaction de membrane nanoporeuse pour reactions miniaturisees et reactions cinetiques ameliorees

Country Status (3)

Country Link
AU (1) AU2002239908A1 (fr)
CA (1) CA2434469A1 (fr)
WO (2) WO2002056004A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2439233B (en) * 2003-09-22 2008-04-23 Shimadzu Corp An electrophoretic separation method
GB2422908B (en) * 2003-09-22 2007-11-21 Shimadzu Corp Electrophoretic member electrophoretic device electrophoretic method and sample dispensing probe
CN100447567C (zh) * 2004-09-22 2008-12-31 杭州生源医疗保健技术开发有限公司 构建组合离子膜微电色谱的方法
US8206974B2 (en) 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
WO2008124104A1 (fr) 2007-04-04 2008-10-16 Network Biosystems Inc. Système intégré pour l'analyse d'acides nucléiques
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
US7964843B2 (en) 2008-07-18 2011-06-21 The George Washington University Three-dimensional molecular imaging by infrared laser ablation electrospray ionization mass spectrometry
US8901487B2 (en) 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
WO2010147654A2 (fr) 2009-06-15 2010-12-23 Netbio Inc. Procédés perfectionnés pour une quantification d'adn à des fins médicolégales
EP2732457A4 (fr) 2011-07-14 2015-09-16 Univ George Washington Collimation de panaches pour spectrométrie de masse avec ionisation par électropulvérisation en ablation au laser
GB2502953B (en) 2012-05-24 2017-02-08 Imp Innovations Ltd Catalytic converter substrate
NL2009191C2 (en) * 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB2517951A (en) 2013-09-05 2015-03-11 Imp Innovations Ltd A substrate and a method of manufacturing a substrate
US10115577B1 (en) * 2017-09-07 2018-10-30 California Institute Of Technology Isotope ratio mass spectrometry

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2560220A1 (fr) * 1984-02-27 1985-08-30 Int Biotechnologies In Dispositif d'electrophorese a gel horizontal
EP0492769A2 (fr) * 1990-12-20 1992-07-01 Wisconsin Alumni Research Foundation Appareil d'électrophorèse en gel horizontal
US5338426A (en) * 1992-08-28 1994-08-16 Applied Biosystems, Inc. Horizontal polyacrylamide gel electrophoresis
EP0911631A1 (fr) * 1991-01-04 1999-04-28 Sebia Dispositif pour l'application d'échantillons biologiques sur une plaque d'électrophorèse
WO2000065336A1 (fr) * 1999-04-26 2000-11-02 Mj Research, Inc. Ensemble d'electrophorese et procede de coulage de gels d'electrophorese
WO2000068677A1 (fr) * 1999-05-06 2000-11-16 University Of Southampton Gel d'electrophorese

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5990373A (en) * 1996-08-20 1999-11-23 Kansas State University Research Foundation Nanometer sized metal oxide particles for ambient temperature adsorption of toxic chemicals
US6057488A (en) * 1998-09-15 2000-05-02 Nantek, Inc. Nanoparticles for the destructive sorption of biological and chemical contaminants

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2560220A1 (fr) * 1984-02-27 1985-08-30 Int Biotechnologies In Dispositif d'electrophorese a gel horizontal
EP0492769A2 (fr) * 1990-12-20 1992-07-01 Wisconsin Alumni Research Foundation Appareil d'électrophorèse en gel horizontal
EP0911631A1 (fr) * 1991-01-04 1999-04-28 Sebia Dispositif pour l'application d'échantillons biologiques sur une plaque d'électrophorèse
US5338426A (en) * 1992-08-28 1994-08-16 Applied Biosystems, Inc. Horizontal polyacrylamide gel electrophoresis
WO2000065336A1 (fr) * 1999-04-26 2000-11-02 Mj Research, Inc. Ensemble d'electrophorese et procede de coulage de gels d'electrophorese
WO2000068677A1 (fr) * 1999-05-06 2000-11-16 University Of Southampton Gel d'electrophorese

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ERFLE H ET AL: "SIMULTANEOUS LOADING OF 200 SAMPLE LANES FOR DNA SEQUENCING ON VERTICAL AND HORIZONTAL, STANDARD AND ULTRATHIN GELS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 11, 1997, pages 2229 - 2230, XP000858128, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
AU2002239908A1 (en) 2002-07-24
WO2002055189A3 (fr) 2004-10-28
WO2002056004A8 (fr) 2003-01-16
WO2002056004A2 (fr) 2002-07-18
WO2002055189A2 (fr) 2002-07-18
CA2434469A1 (fr) 2002-07-18

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