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WO2001007911A3 - A novel method for the improved isolation of a target cell population - Google Patents

A novel method for the improved isolation of a target cell population Download PDF

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Publication number
WO2001007911A3
WO2001007911A3 PCT/DK2000/000408 DK0000408W WO0107911A3 WO 2001007911 A3 WO2001007911 A3 WO 2001007911A3 DK 0000408 W DK0000408 W DK 0000408W WO 0107911 A3 WO0107911 A3 WO 0107911A3
Authority
WO
WIPO (PCT)
Prior art keywords
target cell
cell population
cells
sample
novel method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2000/000408
Other languages
French (fr)
Other versions
WO2001007911A2 (en
Inventor
Britta Christensen
Steen Koelvraa
John Philip
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU59671/00A priority Critical patent/AU5967100A/en
Publication of WO2001007911A2 publication Critical patent/WO2001007911A2/en
Publication of WO2001007911A3 publication Critical patent/WO2001007911A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/505—Erythropoietin [EPO]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70582—CD71

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for the isolation of at least one target cell population from a liquid sample of cells. The method comprises establishing a sample flow over a surface coated with antibodies specific for the target cell population, and allowing the cell sample and surface to be in physical contact. This provides for the cells from the at least one mammalian target cell population to bind to the antibodies on the surface. Then the liquid sample comprising non-bound cells is removed from the surface, and target cells on the surface are identified. The invention further relates to the use of such a method, and to a system for the isolation of target cell populations from a liquid cell sample.
PCT/DK2000/000408 1999-07-21 2000-07-18 A novel method for the improved isolation of a target cell population Ceased WO2001007911A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU59671/00A AU5967100A (en) 1999-07-21 2000-07-18 A novel method for the improved isolation of a target cell population

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199901052 1999-07-21
DKPA199901052 1999-07-21

Publications (2)

Publication Number Publication Date
WO2001007911A2 WO2001007911A2 (en) 2001-02-01
WO2001007911A3 true WO2001007911A3 (en) 2001-11-08

Family

ID=8100444

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2000/000408 Ceased WO2001007911A2 (en) 1999-07-21 2000-07-18 A novel method for the improved isolation of a target cell population

Country Status (2)

Country Link
AU (1) AU5967100A (en)
WO (1) WO2001007911A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7350741B2 (en) * 2017-12-01 2023-09-26 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー Methods for cell enrichment and isolation

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990006509A1 (en) * 1988-12-06 1990-06-14 Flinders Technologies Pty. Ltd. Isolation of fetal cells from maternal blood to enable prenatal diagnosis
WO1993023754A1 (en) * 1992-05-16 1993-11-25 Dorothee Ahlert Process for the prenatal diagnosis of genetic abnormalities
US5437987A (en) * 1992-09-25 1995-08-01 The Board Of Trustees Of The Leland Stanford Junior University Triple gradient process with antibody panning to recover nucleated fetal cells from maternal blood
EP0701130A2 (en) * 1989-06-29 1996-03-13 Applied Immunesciences, Inc. Device and process for cell capture and recovery

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990006509A1 (en) * 1988-12-06 1990-06-14 Flinders Technologies Pty. Ltd. Isolation of fetal cells from maternal blood to enable prenatal diagnosis
EP0701130A2 (en) * 1989-06-29 1996-03-13 Applied Immunesciences, Inc. Device and process for cell capture and recovery
WO1993023754A1 (en) * 1992-05-16 1993-11-25 Dorothee Ahlert Process for the prenatal diagnosis of genetic abnormalities
US5437987A (en) * 1992-09-25 1995-08-01 The Board Of Trustees Of The Leland Stanford Junior University Triple gradient process with antibody panning to recover nucleated fetal cells from maternal blood

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WYSOCKI L J ET AL: "Panning" for lymphocytes: a method for cell selection", PROC. NATL. ACAD. SCI. USA, vol. 75, no. 6, June 1978 (1978-06-01), pages 2844 - 2848, XP002901409 *

Also Published As

Publication number Publication date
WO2001007911A2 (en) 2001-02-01
AU5967100A (en) 2001-02-13

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