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WO2001006008A3 - Deplacement de brins multiplexes pour determinations d'acide nucleique - Google Patents

Deplacement de brins multiplexes pour determinations d'acide nucleique Download PDF

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Publication number
WO2001006008A3
WO2001006008A3 PCT/US2000/018301 US0018301W WO0106008A3 WO 2001006008 A3 WO2001006008 A3 WO 2001006008A3 US 0018301 W US0018301 W US 0018301W WO 0106008 A3 WO0106008 A3 WO 0106008A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
determinations
multiplexed
oligonucleotides
release
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/018301
Other languages
English (en)
Other versions
WO2001006008A2 (fr
Inventor
Sharat Singh
Anita Inamdar
Edwin F Ullman
Liching Cao
David Albagli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monogram Biosciences Inc
Original Assignee
Aclara Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc filed Critical Aclara Biosciences Inc
Priority to EP00948570A priority Critical patent/EP1200624A2/fr
Priority to CA002379749A priority patent/CA2379749A1/fr
Priority to JP2001511216A priority patent/JP2003505036A/ja
Publication of WO2001006008A2 publication Critical patent/WO2001006008A2/fr
Publication of WO2001006008A3 publication Critical patent/WO2001006008A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Des déterminations multiplexées de grands nombres d'événements sont obtenues d'une manière précise et simple à l'aide d'une combinaison de différents oligonucléotides, lesquels servent à la fois à la capture et à la libération sur et à partir d'un support, et des oligonucléotides de déplacement de brins destinés à la libération séquentielle de groupes d'oligonucléotides. Sont également compris, faisant partie des réactifs oligonucléotidiques, des identificateurs, lesquels servent à identifier une caractéristique particulière. La méthode est illustrée à l'aide d'amorces pour l'amorçage de prolongement de chaîne joints à ou servant de séquence de capture/libération, l'amorce prolongée ayant un identificateur. Après prolongement de l'amorce, les amorces prolongées sont capturées et libérées indépendamment et les amorces prolongées libérées sont dosées. La méthode de l'invention trouve une application dans des déterminations de séquences d'acide nucléique, des déterminations de polymorphismes mononucléotidiques, l'identification de fragments d'acide nucléique et analogue.
PCT/US2000/018301 1999-07-16 2000-06-30 Deplacement de brins multiplexes pour determinations d'acide nucleique Ceased WO2001006008A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00948570A EP1200624A2 (fr) 1999-07-16 2000-06-30 Deplacement de brins multiplexes pour determinations d'acide nucleique
CA002379749A CA2379749A1 (fr) 1999-07-16 2000-06-30 Deplacement de brins multiplexes pour determinations d'acide nucleique
JP2001511216A JP2003505036A (ja) 1999-07-16 2000-06-30 核酸決定のための多重鎖置換

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35462999A 1999-07-16 1999-07-16
US09/354,629 1999-07-16

Publications (2)

Publication Number Publication Date
WO2001006008A2 WO2001006008A2 (fr) 2001-01-25
WO2001006008A3 true WO2001006008A3 (fr) 2001-07-12

Family

ID=23394232

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/018301 Ceased WO2001006008A2 (fr) 1999-07-16 2000-06-30 Deplacement de brins multiplexes pour determinations d'acide nucleique

Country Status (4)

Country Link
EP (1) EP1200624A2 (fr)
JP (1) JP2003505036A (fr)
CA (1) CA2379749A1 (fr)
WO (1) WO2001006008A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002029109A2 (fr) * 2000-10-05 2002-04-11 Aclara Biosciences, Inc. Deplacement differentiel multiplexe pour determinations d'acides nucleiques
JP4839051B2 (ja) * 2004-10-08 2011-12-14 シスメックス株式会社 核酸プローブを用いる被検物質の検出方法
DE602005018316D1 (de) * 2004-10-08 2010-01-28 Sysmex Corp Verfahren zum Nachweis einer Zielsubstanz unter Verwendung einer Nukleinsäuresonde
WO2024123917A1 (fr) * 2022-12-07 2024-06-13 Illumina, Inc. Regroupement monoclonal par exclusion stérique d'adn double brin avec ensemencement à motifs

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003911A1 (fr) * 1985-12-17 1987-07-02 Genetics Institute, Inc. Procede d'analyse de polynucleotides base sur le deplacement des brins, et complexe reactif
EP0269764A1 (fr) * 1986-12-01 1988-06-08 Molecular Biosystems, Inc. Procédé pour augmenter la sensibilité des essais d'hybridation des acides nucléiques
US4818680A (en) * 1985-12-18 1989-04-04 Mary Collins Method and kit involving displacement and rehybridization of labeled polynucleotide
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
WO1998014610A2 (fr) * 1996-10-04 1998-04-09 The Perkin-Elmer Corporation Procedes et compositions de capture de polynucleotides multiplex
EP0871774A1 (fr) * 1995-07-25 1998-10-21 University Of Strathclyde Detection d'acides nucleiques et d'unites d'acides nucleiques
WO1999024612A2 (fr) * 1997-11-06 1999-05-20 Mosaic Technologies Reactions multiples de deplacement polynucleotidique sequentiel pour amplification et traitement de signaux

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003911A1 (fr) * 1985-12-17 1987-07-02 Genetics Institute, Inc. Procede d'analyse de polynucleotides base sur le deplacement des brins, et complexe reactif
US4818680A (en) * 1985-12-18 1989-04-04 Mary Collins Method and kit involving displacement and rehybridization of labeled polynucleotide
EP0269764A1 (fr) * 1986-12-01 1988-06-08 Molecular Biosystems, Inc. Procédé pour augmenter la sensibilité des essais d'hybridation des acides nucléiques
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
EP0871774A1 (fr) * 1995-07-25 1998-10-21 University Of Strathclyde Detection d'acides nucleiques et d'unites d'acides nucleiques
WO1998014610A2 (fr) * 1996-10-04 1998-04-09 The Perkin-Elmer Corporation Procedes et compositions de capture de polynucleotides multiplex
WO1999024612A2 (fr) * 1997-11-06 1999-05-20 Mosaic Technologies Reactions multiples de deplacement polynucleotidique sequentiel pour amplification et traitement de signaux

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WOLCOTT M J: "ADVANCES IN NUCLEIC ACID-BASED DETECTION METHODS", CLINICAL MICROBIOLOGY REVIEWS,US,WASHINGTON, DC, vol. 5, no. 4, 1 October 1992 (1992-10-01), pages 370 - 386, XP000606216, ISSN: 0893-8512 *

Also Published As

Publication number Publication date
WO2001006008A2 (fr) 2001-01-25
JP2003505036A (ja) 2003-02-12
EP1200624A2 (fr) 2002-05-02
CA2379749A1 (fr) 2001-01-25

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