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WO2001006003A3 - Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits - Google Patents

Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits Download PDF

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Publication number
WO2001006003A3
WO2001006003A3 PCT/EP2000/006842 EP0006842W WO0106003A3 WO 2001006003 A3 WO2001006003 A3 WO 2001006003A3 EP 0006842 W EP0006842 W EP 0006842W WO 0106003 A3 WO0106003 A3 WO 0106003A3
Authority
WO
WIPO (PCT)
Prior art keywords
carrying
strand breaks
kit
single strand
filter plates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/006842
Other languages
German (de)
English (en)
Other versions
WO2001006003A2 (fr
Inventor
Werner E G Mueller
Heinz C Schroeder
Renato Batel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to EP00949369A priority Critical patent/EP1194591A2/fr
Priority to AU62751/00A priority patent/AU6275100A/en
Publication of WO2001006003A2 publication Critical patent/WO2001006003A2/fr
Publication of WO2001006003A3 publication Critical patent/WO2001006003A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne, entre autres, un procédé de détermination de cassures bicaténaires d'ADN, qui comprend les étapes suivantes: a) dépôt des cellules et/ou des tissus à analyser sur des filtres spécifiques; b) lyse des cellules/tissus avec au moins un détergent, au moins une substance chaotrope et au moins un colorant fluorescent fixant spécifiquement un double brin d'ADN; c) élution des morceaux résultant de la cassure d'ADN, libérés par la lyse, à travers les filtres spécifiques; d) évaluation par spectroscopie à fluorescence. Pour un puits, seulement une fraction d'éluat est collectée.
PCT/EP2000/006842 1999-07-19 2000-07-18 Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits Ceased WO2001006003A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00949369A EP1194591A2 (fr) 1999-07-19 2000-07-18 Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits
AU62751/00A AU6275100A (en) 1999-07-19 2000-07-18 Method and kit for determining dna double/single strand breaks and device for carrying out the controlled filtration of, in particular, dna molecules with well filter plates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19933078.6 1999-07-19
DE19933078A DE19933078B4 (de) 1999-07-19 1999-07-19 Verfahren und Kit zur Bestimmung von DNA-Doppel-/Einzelstrangbrüchen und Vorrichtung zum kontrollierten Filtrieren von insbesondere DNA-Moleküle und/oder andere Moleküle/Molekülkomplexe enthaltenden flüssigen Medien mit Well-Filterplatten

Publications (2)

Publication Number Publication Date
WO2001006003A2 WO2001006003A2 (fr) 2001-01-25
WO2001006003A3 true WO2001006003A3 (fr) 2001-12-13

Family

ID=7914823

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/006842 Ceased WO2001006003A2 (fr) 1999-07-19 2000-07-18 Procede et necessaire pour la determination de cassures bicatenaires ou monocatenaires d'adn, et dispositif pour la filtration controlee en particulier de molecules d'adn avec des plaques de filtration a puits

Country Status (4)

Country Link
EP (1) EP1194591A2 (fr)
AU (1) AU6275100A (fr)
DE (1) DE19933078B4 (fr)
WO (1) WO2001006003A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10111853A1 (de) * 2001-03-05 2002-11-14 Wita Proteomics Ag Verfahren und Vorrichtung zur Aufarbeitung von Proteinen in Gelen
US20030202909A1 (en) * 2002-04-29 2003-10-30 Genetix Limited Vacuum manifold and uses thereof
EP1491258B1 (fr) * 2003-06-24 2008-12-03 Millipore Corporation Systeme de vide multifonctionnelle
US7824623B2 (en) 2003-06-24 2010-11-02 Millipore Corporation Multifunctional vacuum manifold

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2618556A1 (fr) * 1987-07-23 1989-01-27 Adria Procede permettant de detecter si un produit alimentaire a ete irradie
WO1997010055A1 (fr) * 1995-09-15 1997-03-20 Beckman Instruments, Inc. Tubulure a vide pour le traitement en laboratoire d'echantillons liquides multiples
DE19724781A1 (de) * 1997-06-12 1998-12-24 Werner E G Prof Dr Mueller Mikro-Methode zur Schnellbestimmung von DNA-Schäden und deren Reparatur unter Verwendung des Fluoreszenzfarbstoffs Picogreen und ihre Anwendung
DE19725894A1 (de) * 1997-06-19 1998-12-24 Biotechnolog Forschung Gmbh Differentielle Vakuumkammer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2618556A1 (fr) * 1987-07-23 1989-01-27 Adria Procede permettant de detecter si un produit alimentaire a ete irradie
WO1997010055A1 (fr) * 1995-09-15 1997-03-20 Beckman Instruments, Inc. Tubulure a vide pour le traitement en laboratoire d'echantillons liquides multiples
DE19724781A1 (de) * 1997-06-12 1998-12-24 Werner E G Prof Dr Mueller Mikro-Methode zur Schnellbestimmung von DNA-Schäden und deren Reparatur unter Verwendung des Fluoreszenzfarbstoffs Picogreen und ihre Anwendung
DE19725894A1 (de) * 1997-06-19 1998-12-24 Biotechnolog Forschung Gmbh Differentielle Vakuumkammer

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DUCORE J.M.: "EDTA alkaline elution characteristics and measurement of DNA damage in unlabeled DNA using Hoechst 33258 fluorescence", ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, vol. 11, 1988, US, pages 449 - 460, XP000995522 *

Also Published As

Publication number Publication date
WO2001006003A2 (fr) 2001-01-25
DE19933078B4 (de) 2004-07-08
DE19933078A1 (de) 2001-02-08
AU6275100A (en) 2001-02-05
EP1194591A2 (fr) 2002-04-10

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