WO2001089314A1 - Method for the processing of plant material - Google Patents
Method for the processing of plant material Download PDFInfo
- Publication number
- WO2001089314A1 WO2001089314A1 PCT/EP2001/005831 EP0105831W WO0189314A1 WO 2001089314 A1 WO2001089314 A1 WO 2001089314A1 EP 0105831 W EP0105831 W EP 0105831W WO 0189314 A1 WO0189314 A1 WO 0189314A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- juice
- separating
- press cake
- polymer
- vegetable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
- A23J1/007—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials from leafy vegetables, e.g. alfalfa, clover, grass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Definitions
- the invention relates to a method for processing plant material, wherein all components of the plant material are separated.
- the object of the present invention is therefore to provide a method for processing vegetable material which is as gentle and inexpensive as possible.
- the solution to the problem is a method for processing plant material, comprising the steps:
- step (c) separating the plant juice obtained in step (b) into a polymer-rich (rieh juice) and a polymer-poor juice,
- step (d) heating the polymer-rich juice obtained in step (c), whereby a conglomerate of proteins and a supernatant are obtained,
- step (e) separating the conglomerate obtained in step (d) from the supernatant
- step (g) separating the low-polymer juice obtained in step (c), thereby obtaining phytominerals and "vicious water".
- Another solution is a feed, obtainable by (a) harvesting vegetable matter, (b) grinding and squeezing the vegetable matter to obtain a press cake and vegetable juice, and (c) separating the press cake obtained in step (b).
- the invention is explained in more detail below with reference to FIG. 1.
- Figure 1 shows a flow diagram of the method according to the invention.
- alfalfa was harvested.
- the plant material thus obtained can then be heated to a temperature for inducing the production of stress proteins (HSP) in the plant material from 38 ° C. to 40 ° C.
- HSP stress proteins
- the alfalfa was then compressed and cooled.
- the plant material made of compressed alfalfa was then transferred to a hammer mill, with which the stems of the alfalfa plants were separated from their leaves and shredded. Then the shredded material was further ground in a mill and pressed with the separation of alfalfa juice and a press cake.
- the alfalfa juice was separated in a cross-flow ultrafiltration system (cross-flow ultra filtration) into a polymer-rich alfalfa juice and a polymer-poor alfalfa juice.
- the polymer-rich alfalfa juice was heated, resulting in a protein conglomerate, which, when the plant material was heated to induce stress protein production, comprised a stress protein conglomerate.
- the protein conglomerate was separated from the liquid phase.
- the liquid phase was then applied to a gel and eluted, whereby a protein, optionally a stress protein with a molecular weight of 65000 (HSP65), was obtained.
- the polymer-poor alfalfa juice was separated into a dilute aqueous phase and a phytomineral-rich phase by means of reverse osmosis.
- the dilute aqueous phase can be used as "invigorating water". This water is very healthy for living things because it comes from plants.
- the press cake or the feed according to the invention is well suited for feeding animals, since it still contains a large number of proteins from the plant material which have not been destroyed by excessive heating.
- An advantage of the method according to the invention is that none of the constituents of the vegetable material is discarded, thus making optimal use of the vegetable material possible.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Fodder In General (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Verfahren zum Verarbeiten von pflanzlichem Material Process for processing plant material
Die Erfindung betrifft ein Verfahren zum Verarbeiten von pflanzlichem Material, wobei alle Bestandteile des pflanzlichen Materials getrennt werden.The invention relates to a method for processing plant material, wherein all components of the plant material are separated.
Herkömmlicherweise wird pflanzliches Material nach dem Schneiden bei bis zu 900°C im Gegenstrom mittels Heißluft getrocknet, um daraus ein Futtermittel herzustellen. Dies ist nachteilig, da dadurch fast alle Proteine in dem pflanzlichen Material zerstört werden und ein hoher Energieaufwand zum Trocknen erforderlich ist.Traditionally, vegetable material is dried in countercurrent using hot air after cutting at up to 900 ° C to produce a feed. This is disadvantageous, since it destroys almost all proteins in the plant material and requires a lot of energy to dry.
Aufgabe der vorliegenden Erfindung ist es daher, ein Verfahren zum Verarbeiten von pflanzlichem Material zur Verfügung zu stellen, das möglichst schonend und kostengünstig ist.The object of the present invention is therefore to provide a method for processing vegetable material which is as gentle and inexpensive as possible.
Die Lösung der Aufgabe ist ein Verfahren zum Aufbereiten von pflanzlichem Material, umfassend die Schritte:The solution to the problem is a method for processing plant material, comprising the steps:
(a) Ernten des pflanzlichen Materials,(a) harvesting the plant material,
(b) Auspressen des pflanzlichen Materials, wodurch ein Presskuchen (press cake) und Pflanzensaft (juice) erhalten werden,(b) squeezing the plant material to obtain a press cake and plant juice,
(c) Trennen des in Schritt (b) erhaltenen Pflanzensafts in einen Polymer-reichen (rieh juice) und einen Polymer-armen Saft,(c) separating the plant juice obtained in step (b) into a polymer-rich (rieh juice) and a polymer-poor juice,
(d) Erwärmen des in Schritt (c) erhaltenen Polymer-reichen Safts, wodurch ein Konglomerat an Proteinen und ein Überstand erhalten werden,(d) heating the polymer-rich juice obtained in step (c), whereby a conglomerate of proteins and a supernatant are obtained,
(e) Abtrennen des in Schritt (d) erhaltenen Konglomerats von dem Überstand,(e) separating the conglomerate obtained in step (d) from the supernatant,
(f) Abtrennen von mindestens einem weiteren Protein aus dem nach Schritt (e) erhaltenen Überstand und(f) separating at least one further protein from the supernatant obtained after step (e) and
(g) Trennen des in Schritt (c) erhaltenen Polymer-armen Safts, wodurch Phytominerale und „belebendes Wasser" (vicious water) erhalten werden.(g) separating the low-polymer juice obtained in step (c), thereby obtaining phytominerals and "vicious water".
Eine weitere Lösung ist ein Futtermittel, erhältlich durch (a) Ernten von pflanzlichem Material, (b) Mahlen und Auspressen des pflanzlichen Materials, wodurch ein Presskuchen und Pflanzensaft erhalten werden und (c) Abtrennen des in Schritt (b) erhaltenen Presskuchens. Die Erfindung wird nachstehend anhand der Figur 1 näher erläutert.Another solution is a feed, obtainable by (a) harvesting vegetable matter, (b) grinding and squeezing the vegetable matter to obtain a press cake and vegetable juice, and (c) separating the press cake obtained in step (b). The invention is explained in more detail below with reference to FIG. 1.
Figur 1 zeigt ein Fließbild des erfindungsgemäßen Verfahrens.Figure 1 shows a flow diagram of the method according to the invention.
Wie in Figur 1 gezeigt, wurde Alfalfa geerntet. Sodann kann das so erhaltene pflanzliche Material auf eine Temperatur für die Induktion der Produktion von Stressproteinen (HSP) in dem pflanzlichen Material von 38 °C bis 40 °C erwärmt werden. Anschließend wurde das Alfalfa verdichtet und abgekühlt. Das pflanzliche Material aus verdichtetem Alfalfa wurde sodann in eine Hammermühle überführt, mit der die Stengel der Alfalfa Pflanzen von deren Blättern getrennt und zerkleinert wurden. Dann wurde das zerkleinerte Material in einer Mühle weiter gemahlen und gepresst unter Abtrennung von Alfalfasaft und einem Presskuchen.As shown in Figure 1, alfalfa was harvested. The plant material thus obtained can then be heated to a temperature for inducing the production of stress proteins (HSP) in the plant material from 38 ° C. to 40 ° C. The alfalfa was then compressed and cooled. The plant material made of compressed alfalfa was then transferred to a hammer mill, with which the stems of the alfalfa plants were separated from their leaves and shredded. Then the shredded material was further ground in a mill and pressed with the separation of alfalfa juice and a press cake.
Der Alfalfasaft wurde in einer Kreuzstrom Ultrafiltrationsanlage (cross-Flow Ultra Filtrierung) aufgetrennt in einen Polymer-reichen Alfalfasaft und einen Polymer-armen Alfalfasaft.The alfalfa juice was separated in a cross-flow ultrafiltration system (cross-flow ultra filtration) into a polymer-rich alfalfa juice and a polymer-poor alfalfa juice.
Der Polymer-reiche Alfalfasaft wurde erwärmt, wobei sich ein Protein-Konglomerat, das, wenn das pflanzliche Material zur Induktion der Produktion von Streßproteinen erwärmt wurde, ein Streßprotein-Konglomerat umfasst, niederschlug. Das Protein Konglomerat wurde von der flüssigen Phase abgeschieden. Dann wurde die flüssige Phase auf ein Gel aufgebracht und eluiert, wodurch ein Protein, gegebenfalls ein Stressprotein mit einem Molekulargewicht von 65000 (HSP65), erhalten wurde.The polymer-rich alfalfa juice was heated, resulting in a protein conglomerate, which, when the plant material was heated to induce stress protein production, comprised a stress protein conglomerate. The protein conglomerate was separated from the liquid phase. The liquid phase was then applied to a gel and eluted, whereby a protein, optionally a stress protein with a molecular weight of 65000 (HSP65), was obtained.
Der Polymer-arme Alfalfasaft wurde mittels Umkehrosmose in eine verdünnte wässrige Phase und eine Phytomineral-reiche Phase getrennt.The polymer-poor alfalfa juice was separated into a dilute aqueous phase and a phytomineral-rich phase by means of reverse osmosis.
Die verdünnte wässrige Phase kann als „belebendes Wasser" verwendet werden. Dieses Wasser ist sehr gesund für Lebewesen, da es aus Pflanzen stammt.The dilute aqueous phase can be used as "invigorating water". This water is very healthy for living things because it comes from plants.
Aus 1000 kg Alfalfa, welches etwa 200 g Stressproteine enthält, können beispielsweise 400-600 kg Presskuchen, enthaltend etwa <=120 g Stressproteine, 400-600 Liter Saft, enthaltend etwa <= 80 g Stressproteine und 3-5 Liter an Polymer-reichem Saft erhalten werden, aus dem sich weitere Stressproteine, insbesondere HSP65, isolieren lassen. Außerdem zeigt Tabelle 1 tabellarisch die Arbeitschritte des erfindungsgemäßen Verfahrens sowie die dabei erhaltenen Ausbeuten. Tabelle 2 einen Produktionsablauf des erfindungsgemäßen Verfahrens.From 1000 kg of alfalfa, which contains about 200 g of stress proteins, 400-600 kg of presscake, for example, containing about <= 120 g of stress proteins, 400-600 liters of juice, containing about <= 80 g of stress proteins and 3-5 liters of polymer-rich, for example Juice are obtained from which further stress proteins, in particular HSP65, can be isolated. Table 1 also shows in table form the working steps of the process according to the invention and the yields obtained in the process. Table 2 shows a production sequence of the method according to the invention.
Der Presskuchen bzw. das erfindungsgemäße Futtermittel ist zum Füttern von Tieren gut geeignet, da er noch eine Vielzahl von Proteinen aus dem pflanzlichen Material enthält, die nicht durch starkes Erwärmen zerstört wurden.The press cake or the feed according to the invention is well suited for feeding animals, since it still contains a large number of proteins from the plant material which have not been destroyed by excessive heating.
Ein Vorteil des erfindungsgemäßen Verfahrens ist, dass keiner der Bestandteile des pflanzlichen Materials verworfen wird und somit eine optimale Nutzung des pflanzlichen Materials ermöglicht wird. An advantage of the method according to the invention is that none of the constituents of the vegetable material is discarded, thus making optimal use of the vegetable material possible.
ArbeitsschrittθArbeitsschrittθ
Ernte 30 sec Wärmebehandlung = > Geschwindigkeit geerntete Flache bei ErntebreiteHarvest 30 sec heat treatment => speed harvested area at harvest width
Ertrag / ha Erntemenge: Yield / ha harvest:
Ausbeuteberechnung:Yield calculation:
Anreicherungsfaktor Potential Ausbeutegrad Ausbeute Enrichment factor potential degree of yield yield
Durchsatzberechnungen:Throughput Calculations:
Input 1 176 kg AlfalfaInput 1 176 kg alfalfa
Hammermuhle Anteil StämmchenHammer mill share stem
1000 Blatter 176,5 kg Stammchen1000 leaves 176.5 kg stem
Schneckenmuhle Input 1000 RestfeuchteSchneckenmuhle Input 1000 residual moisture
t FuMi t FuMi
Cross-Flow-Ultra-Filtration InputCross-flow ultra filtration input
Anteil an Rich-Juice OutputProportion of rich juice output
Erhitzung + Filtration Input 3 kgHeating + Filtration Input 3 kg
Anteil Prozentual 1 ,67% HSP in Rieh JuicePercentage 1.67% HSP in Rieh Juice
Ausbeute 60,0%Yield 60.0%
Output 0,03 kg HSP kg HSP Output 0.03 kg HSP kg HSP
Tabelle 2Table 2
Verarbeitungsslimit in to/h Tage pro Woche 5 Wochen pro Monat 4 Produktionsstunden / tag Wochen pro Jahr 20Processing limit in to / h days per week 5 weeks per month 4 production hours / day Weeks per year 20
Produktion pro Tag pro Woche pro Monat pro JahrProduction per day per week per month per year
Erntemaschine Laufzeit der Maschine 5,23 26, 14 104,58 522,88Harvester runtime of the machine 5.23 26, 14 104.58 522.88
Input Alfalfa 75,29 376,47 1505,88 7529,411Input alfalfa 75.29 376.47 1505.88 7529.411
Hammermühle Stämmchen 1 1 ,29 56,47 225,88 1 129,41Hammer mill trunks 1 1, 29 56.47 225.88 1 129.41
Outputoutput
Blätter 64,00 320,00 1280,00 6400,00Sheets 64.00 320.00 1280.00 6400.00
Input Blätter 64,00 320,00 1280,00 6400,00Input sheets 64.00 320.00 1280.00 6400.00
Schneckenmühlθ Cake 28,80 144,00 576,00 2880,00Schneckenmühlθ Cake 28.80 144.00 576.00 2880.00
Outputoutput
Saft 35,20 176,00 704,00 3520,00Juice 35.20 176.00 704.00 3520.00
Input Saft 35,20 176,00 704,00 3520,00Input juice 35.20 176.00 704.00 3520.00
Cross-Flow-Ultra-Filtration Rest-Saft 35,01 175,04 700, 16 3500,80Cross-flow ultra-filtration residual juice 35.01 175.04 700, 16 3500.80
Outputoutput
Rich-Juice 0, 19 0,96 3,84 19,20Rich juice 0, 19 0.96 3.84 19.20
Input Rich-Juice 0, 19 0,96 3,84 19,20Input rich juice 0, 19 0.96 3.84 19.20
Erhitzung + FiltrationHeating + filtration
Output HSP in kg 1 ,92 9,60 38,40 192,00|Output HSP in kg 1, 92 9.60 38.40 192.00 |
HSP in kg 1 ,92 9,60 38,40 192,00HSP in kg 1, 92 9.60 38.40 192.00
FuMi in Tonnen 40,09FuMi in tons 40.09
Outputs 200,47 801 ,88 4009,41Outputs 200.47 801, 88 4009.41
Phytominerals in kg 64,00 320,00 1280,00 6400,00Phytominerals in kg 64.00 320.00 1280.00 6400.00
Vicious Water in Hekto-Liter 349,44 1747,20 6988,80 34944,00 Vicious Water in hecto-liters 349.44 1747.20 6988.80 34944.00
Claims
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU58403/01A AU5840301A (en) | 2000-05-19 | 2001-05-21 | Method for the processing of plant material |
| EP01931699A EP1282360A1 (en) | 2000-05-19 | 2001-05-21 | Method for the processing of plant material |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10024838.1 | 2000-05-19 | ||
| DE10024838A DE10024838A1 (en) | 2000-05-19 | 2000-05-19 | Processing of plant material (e.g. alfalfa) to make use of all the components and to give an animal feed, together with high yields of non-degraded stress proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2001089314A1 true WO2001089314A1 (en) | 2001-11-29 |
Family
ID=7642803
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2001/005831 Ceased WO2001089314A1 (en) | 2000-05-19 | 2001-05-21 | Method for the processing of plant material |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1282360A1 (en) |
| AU (1) | AU5840301A (en) |
| DE (1) | DE10024838A1 (en) |
| WO (1) | WO2001089314A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3160254A4 (en) * | 2014-06-26 | 2018-04-11 | Biotest ApS | A method of providing functional proteins from a plant material |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102006050619B4 (en) * | 2006-10-26 | 2015-03-05 | Emsland-Stärke GmbH | A method for obtaining legume protein fractions, legume protein fraction and use thereof |
| DE102006050620A1 (en) * | 2006-10-26 | 2008-05-08 | Emsland-Stärke GmbH | A method of obtaining medium molecular weight vegetable protein fractions, vegetable protein fraction and use thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3684520A (en) * | 1969-12-09 | 1972-08-15 | Us Agriculture | Fractionation of leafy green crops |
| DE2832883A1 (en) * | 1978-07-27 | 1980-02-14 | Wieneke Franz | Protein concentrate recovery from green plant juice - by coagulating solids in juice, concn. e.g. by capillary filtration and drying |
| WO2000070931A1 (en) * | 1999-05-19 | 2000-11-30 | Ibrtn Institut Für Bio-Rationale Technologie Nordhorn Gmbh | Method for producing stress proteins from vegetable material and harvesting machine for implementing said method |
-
2000
- 2000-05-19 DE DE10024838A patent/DE10024838A1/en not_active Withdrawn
-
2001
- 2001-05-21 AU AU58403/01A patent/AU5840301A/en not_active Abandoned
- 2001-05-21 WO PCT/EP2001/005831 patent/WO2001089314A1/en not_active Ceased
- 2001-05-21 EP EP01931699A patent/EP1282360A1/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3684520A (en) * | 1969-12-09 | 1972-08-15 | Us Agriculture | Fractionation of leafy green crops |
| DE2832883A1 (en) * | 1978-07-27 | 1980-02-14 | Wieneke Franz | Protein concentrate recovery from green plant juice - by coagulating solids in juice, concn. e.g. by capillary filtration and drying |
| WO2000070931A1 (en) * | 1999-05-19 | 2000-11-30 | Ibrtn Institut Für Bio-Rationale Technologie Nordhorn Gmbh | Method for producing stress proteins from vegetable material and harvesting machine for implementing said method |
| WO2000070932A1 (en) * | 1999-05-19 | 2000-11-30 | Jaliashvili Tengiz A | Method for induction synthesis of heat stress proteins (hsp) & production from herbaceous plants |
Non-Patent Citations (2)
| Title |
|---|
| DOLORES V. LAYUG ET AL.: "Effect of maturity stage on the protein and carotenoid yields of alfalfa leaf extract and press cake", GRASSLAND SCIENCE, vol. 41, no. 4, 1996, pages 287 - 293, XP000982892 * |
| RICHARD H. EDWARDS ET AL.: "Use of recycled dilute juice solubles to increase the yield of leaf protein concentrate from alfalfa", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY., vol. 26, no. 3, 1978, AMERICAN CHEMICAL SOCIETY. WASHINGTON., US, pages 738 - 741, XP002162190, ISSN: 0021-8561 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3160254A4 (en) * | 2014-06-26 | 2018-04-11 | Biotest ApS | A method of providing functional proteins from a plant material |
Also Published As
| Publication number | Publication date |
|---|---|
| AU5840301A (en) | 2001-12-03 |
| EP1282360A1 (en) | 2003-02-12 |
| DE10024838A1 (en) | 2001-11-22 |
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