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WO2001088168A3 - Mapping molecular interactions in plants with protein fragments complementation assays - Google Patents

Mapping molecular interactions in plants with protein fragments complementation assays Download PDF

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Publication number
WO2001088168A3
WO2001088168A3 PCT/US2001/015170 US0115170W WO0188168A3 WO 2001088168 A3 WO2001088168 A3 WO 2001088168A3 US 0115170 W US0115170 W US 0115170W WO 0188168 A3 WO0188168 A3 WO 0188168A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
plants
protein fragments
molecular interactions
complementation assays
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/015170
Other languages
French (fr)
Other versions
WO2001088168A2 (en
Inventor
Stephen W Michnick
Normand Brisson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Odyssey Pharmaceuticals Inc
Original Assignee
Odyssey Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Odyssey Pharmaceuticals Inc filed Critical Odyssey Pharmaceuticals Inc
Priority to AU2001261408A priority Critical patent/AU2001261408A1/en
Publication of WO2001088168A2 publication Critical patent/WO2001088168A2/en
Publication of WO2001088168A3 publication Critical patent/WO2001088168A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

he present invention describes a method of expressing PCA interacting partners in plant material comprising: (A) transforming said material with: (1) a first construct coding for a first fusion product comprising (a) a first fragment of a first molecule whose fragments can exhibit a detectable activity when associated and (b) a first protein-protein interacting domain; and (2) a second construct coding for a second fusion product comprising (a) a second fragment of said first molecule and (b) a second protein-protein interacting domain that can bind (1)(b); (B) culturing said material under conditions allowing expression of said PCA interacting partners; and (C) detecting said activity.
PCT/US2001/015170 2000-05-12 2001-05-11 Mapping molecular interactions in plants with protein fragments complementation assays Ceased WO2001088168A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001261408A AU2001261408A1 (en) 2000-05-12 2001-05-11 Mapping molecular interactions in plants with protein fragments complementation assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20393700P 2000-05-12 2000-05-12
US60/203,937 2000-05-12

Publications (2)

Publication Number Publication Date
WO2001088168A2 WO2001088168A2 (en) 2001-11-22
WO2001088168A3 true WO2001088168A3 (en) 2002-04-25

Family

ID=22755898

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/015170 Ceased WO2001088168A2 (en) 2000-05-12 2001-05-11 Mapping molecular interactions in plants with protein fragments complementation assays

Country Status (2)

Country Link
AU (1) AU2001261408A1 (en)
WO (1) WO2001088168A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020064769A1 (en) * 2000-10-05 2002-05-30 Watson Michnick Stephen William Dynamic visualization of expressed gene networks in living cells
EP1601576A4 (en) * 2003-03-03 2007-12-05 Univ Columbia LIGAND BIOMARKETING SYSTEMS / LIAISON PARTNER
JP5933700B2 (en) * 2011-06-07 2016-06-15 フラウンホーファー−ゲゼルシャフト ツル フェルデルング デル アンゲヴァンテン フォルシュング エー ファウＦｒａｕｎｈｏｆｅｒ−Ｇｅｓｅｌｌｓｃｈａｆｔ ｚｕｒ Ｆｏｅｒｄｅｒｕｎｇ ｄｅｒ ａｎｇｅｗａｎｄｔｅｎ Ｆｏｒｓｃｈｕｎｇ ｅ．Ｖ． Methods for generating monoclonal plant cell lines
WO2024103117A1 (en) * 2022-11-16 2024-05-23 Commonwealth Scientific And Industrial Research Organisation Protoplast screening assay

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034120A1 (en) * 1997-01-31 1998-08-06 Universite De Montreal Protein fragment complementation assays to detect biomolecular interactions
WO1998037211A1 (en) * 1997-02-21 1998-08-27 Gene Shears Pty. Limited Protein complementation in transgenic plants
WO1998044350A1 (en) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
WO2000007038A2 (en) * 1998-07-30 2000-02-10 Universite De Montreal Protein fragment complementation assays
WO2000071701A1 (en) * 1999-05-24 2000-11-30 New England Biolabs, Inc. Method for generating split, non-transferable genes that are able to express an active protein product
WO2001059091A2 (en) * 2000-02-11 2001-08-16 Metabolix, Inc. Multi-gene expression constructs containing modified inteins
WO2001094617A2 (en) * 2000-06-02 2001-12-13 Odyssey Pharmaceuticals, Inc. Protein fragment complementation assay based on beta-lactamase

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034120A1 (en) * 1997-01-31 1998-08-06 Universite De Montreal Protein fragment complementation assays to detect biomolecular interactions
WO1998037211A1 (en) * 1997-02-21 1998-08-27 Gene Shears Pty. Limited Protein complementation in transgenic plants
WO1998044350A1 (en) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
WO2000007038A2 (en) * 1998-07-30 2000-02-10 Universite De Montreal Protein fragment complementation assays
WO2000071701A1 (en) * 1999-05-24 2000-11-30 New England Biolabs, Inc. Method for generating split, non-transferable genes that are able to express an active protein product
WO2001059091A2 (en) * 2000-02-11 2001-08-16 Metabolix, Inc. Multi-gene expression constructs containing modified inteins
WO2001094617A2 (en) * 2000-06-02 2001-12-13 Odyssey Pharmaceuticals, Inc. Protein fragment complementation assay based on beta-lactamase

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Clonal selection and mapping of the functional relationships among plant genes with a protein fragment complementation strategy", 1999-2000 GENOMICS PROJECTS COMPETITION RESULTS - NSERC, XP002189156, Retrieved from the Internet <URL:http://www.nserc.ca/programs/genres00_e.htm> *
PELLETIER, J.F., ET AL: "An in vivo library-versus-library selection of optimized protein-protein interactions", NATURE BIOTECHNOLOGY, vol. 17, July 1999 (1999-07-01), pages 663 - 690, XP002189201 *
REMY I ET AL: "CLONAL SELECTION AND IN VIVO QUANTITATION OF PROTEIN INTERACTONS WITH PROTEIN-FRAGMENT COMPLEMENTATION ASSAYS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, no. 10, 11 May 1999 (1999-05-11), pages 5394 - 5399, XP001008795, ISSN: 0027-8424 *
SUBRAMANIAN, R., ET AL.: "Direct visualization of protein interactions in plant cells", NATURE BIOTECHNOLOGY, vol. 19, August 2001 (2001-08-01), pages 769 - 772, XP002189157 *

Also Published As

Publication number Publication date
AU2001261408A1 (en) 2001-11-26
WO2001088168A2 (en) 2001-11-22

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