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WO2001066947A1 - Systeme de distribution de liquide et dispositif associe - Google Patents

Systeme de distribution de liquide et dispositif associe Download PDF

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Publication number
WO2001066947A1
WO2001066947A1 PCT/JP2000/001332 JP0001332W WO0166947A1 WO 2001066947 A1 WO2001066947 A1 WO 2001066947A1 JP 0001332 W JP0001332 W JP 0001332W WO 0166947 A1 WO0166947 A1 WO 0166947A1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid
chamber
valve
inlet
diaphragm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2000/001332
Other languages
English (en)
Japanese (ja)
Inventor
Yasuhiko Sasaki
Akira Koide
Yasuhiro Yoshimura
Ryo Miyake
Takao Terayama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to PCT/JP2000/001332 priority Critical patent/WO2001066947A1/fr
Publication of WO2001066947A1 publication Critical patent/WO2001066947A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/02—Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
    • F04B43/04—Pumps having electric drive
    • F04B43/043—Micropumps
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1039—Micropipettes, e.g. microcapillary tubes

Definitions

  • the present invention relates to a diaphragm-type micropump, and more particularly, to a micropump of several seconds per second.
  • the present invention relates to a liquid sending device that sends liquid from L to several hundreds / L and an analyzer using the same.
  • a micro-diaphragm pump is a diaphragm pump with a size of several tens of millimeters or less, and the volume of the liquid transfer chamber is changed by deforming the diaphragm that forms part of the liquid transfer chamber.
  • the liquid is introduced into the liquid feed chamber from the inlet, and by reducing the volume, the liquid is discharged from the outlet.
  • the most common microdiaphragm pump processes single-crystal silicon using an aqueous alkali solution (anisotropic etching technology), and joins single-crystal silicon and pyrex glass (positive bonding technology). They are assembled and manufactured.
  • the former diaphragm pump consists of three chambers: an inlet valve chamber, a liquid feed chamber, and an outlet valve chamber.
  • the position of the inlet from which the liquid enters the liquid feed chamber during priming is located at the center of the liquid feed chamber.
  • an aqueous solution containing at least one kind of water-soluble salt or polyhydric alcohol is once injected into the pump and dried, whereby the water-soluble salt or polyhydric alcohol is applied to the surface of the pump in contact with the liquid.
  • Adhesives are attached to improve the affinity for liquid, eliminating bubbles remaining during priming.
  • bubbles may remain in the initial liquid introduction. This is due to the shape of the flow path from the inlet valve to the liquid through the liquid feed chamber to the outlet valve, and particularly air bubbles remain near the valve structure.
  • an object of the present invention is to provide a diaphragm pump that suppresses the generation of residual air bubbles and realizes highly accurate liquid sending.
  • the step near the inlet valve is reduced, and the inlet is arranged at the outer periphery of the flow path plane shape so that the outer periphery side is opened to the greatest extent.
  • FIG. 1 is a schematic diagram of the first embodiment of the present invention
  • FIG. 2 is a diagram showing the relationship between the liquid sending chamber height and the remaining bubbles in the first embodiment of the present invention.
  • 3 is a schematic diagram of the second embodiment of the present invention
  • FIG. 4 is a schematic diagram of the third embodiment of the present invention
  • FIG. 5 is an analyzer of the liquid sending device of the present invention.
  • FIG. 6 is a detailed explanatory view of the first application example of the present invention
  • FIG. 7 is a second application example of the liquid supply apparatus of the present invention to the analysis apparatus.
  • FIG. 8 shows a third application example of the liquid sending device of the present invention to an analyzer.
  • FIG. 1 is a schematic diagram of a liquid feeding device according to a first embodiment of the present invention.
  • (a) is a cross-sectional view of the liquid sending device
  • (b) is a plan view of the liquid sending chamber.
  • the liquid transfer device is composed of three substrates: a diaphragm substrate 110, a liquid transfer chamber substrate 120, and an inlet / outlet substrate 130.
  • the material of these substrates is silicon, glass, ceramic, metal or resin.
  • the liquid supply chamber substrate 120 has a liquid supply chamber 121, an inlet valve 122, and an outlet port 123.
  • the outlet valve substrate 130 has an outlet valve 132, and an inlet port 1 3 3, suction port 1 3 4 and discharge port 1 3 5 are machined. Machining methods include photolithography, sand blasting, injection molding, honing, electric discharge machining, and electric power.
  • the substrate and the piezoelectric disk 112 serving as an actuator are fixed to each other to assemble a liquid sending device.
  • the fixing method is direct bonding, anodic bonding, diffusion bonding, eutectic bonding, brazing, surface activated bonding, Use at least one.
  • each part is as follows.
  • the thickness of the diaphragm substrate is 500 ⁇ m
  • the thickness of the liquid transfer chamber substrate is 500, "m
  • the thickness of the inlet / outlet substrate is 500 ⁇ m.
  • the height from the bottom of the liquid transfer chamber 1 2 1 to the diaphragm surface 1 2 9 (hereinafter referred to as the liquid transfer chamber height) is 500 / m, and the height from the inlet port 1 33 to the diaphragm surface.
  • the height 1 28 (hereinafter referred to as the height above the inlet) is 600 / im
  • the width 3 2 1 in the plan view of the liquid transfer chamber 1 2 1 is 8 mm
  • the length 3 2 2 is 9 mm.
  • the external dimensions in the plan view are 14 mm x 14 mm.
  • the shape of the liquid transfer chamber was gradually expanded from the valve part on the inlet side, with a parallel part in the middle, and formed so as to gradually reduce from the parallel part toward the outlet side. .
  • the inlet valve 122 has a cantilever structure supported by beams 124.
  • the beam 124 is provided toward the inside of the liquid sending chamber 121, and the inlet valve 122 is arranged close to one side of the liquid sending chamber 121. Further, an outlet port 123 is arranged close to the side opposite to the side on which the inlet valve 122 is close.
  • the outlet valve 13 2 has a doubly supported structure supported by two beams (not shown).
  • the priming procedure of this liquid feeding device is as follows.
  • a liquid introducing device for sending the liquid to be introduced is connected to the suction port 134 of the liquid sending device.
  • This liquid introduction device uses one agricultural land such as a syringe pump, a tube pump, and a gear pump.
  • the pressurized liquid reaches the inlet valve 1 2 2 through the inlet port 1 3 3, and the pressure increases.
  • the inlet valve 1 2 2 is opened and the liquid flows into the liquid supply chamber 1 2 1.
  • the inlet valve 1 2 2 supported by the beam 1 2 4 passes through the inlet valve 1 2 2 as indicated by the arrow in Fig. 1 (b) because it opens at the root of the beam 1 2 4 at the fulcrum.
  • the filled liquid fills the liquid transfer chamber 1 2 1 from the adjacent side. Then reach exit port 1 2 3.
  • the liquid as well as the gas opens the inlet valve 122, and the liquid flows from the inlet into the liquid supply chamber 122 and reaches the outlet port 123.
  • the suction pump connected to the discharge port 1 35 is separated, and the preparation for liquid transfer is completed.
  • the bombing operation is performed by applying a voltage to the piezoelectric disks 1 1 and 2 to expand and contract in the plane direction.
  • the piezoelectric disk 112 is contracted in the plane direction and the diaphragm 111 is pushed into the liquid supply chamber side like a drum, the volume of the liquid supply chamber 121 decreases.
  • the reduced volume of the liquid is pushed out of the outlet valve 13 2, flows out of the outlet port 12 3 to the outside of the liquid sending chamber 12 1, and is discharged from the discharge outlet 13 5.
  • the diaphragm 111 returns to the piezoelectric disk side, and the volume of the liquid sending chamber 122 becomes large. Then, the liquid corresponding to the increased volume pushes open the inlet valve 122 and flows into the liquid sending chamber 122 from the inlet port 133. The liquid is fed by repeating this operation.
  • the first feature is that the liquid transfer chamber height is 1 2 9 to 5 0
  • the point is that the height above the inlet is set to 600 ⁇ m, and the residual air bubbles during priming are prevented.
  • the presence or absence of bubbles in the inlet valve depends on the wettability in the liquid transfer chamber.
  • the wettability in the liquid transfer chamber depends on the surface tension T 8 (N / m) and density p (kg / m 3 ) of the liquid, the contact angle (°) between the liquid and the liquid contact part of the liquid transfer chamber, and the height of the liquid transfer chamber.
  • T 8 N / m
  • density p kg / m 3
  • This threshold value h is constant, and when the physical properties (surface tension and density) of the liquid and the contact angle between the liquid and the liquid contact part of the liquid supply chamber change, the liquid supply chamber height at that time changes. For example, if the wettability between the liquid and the inner surface of the liquid transfer chamber is better than in the case of Fig. 2, the liquid transfer chamber height needs to be set higher. Conversely, when wettability is poor, the height of the liquid transfer chamber can be set small.
  • the outlet port 123 with a higher potential energy than the inlet valve 122 with respect to the horizontal plane based on the direction of gravity, The liquid that has entered the channel receives an attractive force in the direction of gravity due to its own density.
  • this attractive force has a reaction force component with respect to the surface tension
  • the influence of the surface tension in the liquid supply chamber can be reduced.
  • the reaction force component of the attractive force becomes maximum.
  • the air bubbles mixed into the liquid sending chamber 122 move from the inlet valve 122 to the outlet port 123 by its own buoyancy, so that the air bubbles can be easily removed.
  • the line connecting the inlet and the outlet was perpendicular to the horizontal plane with respect to the direction of gravity. The same effect can be obtained if the outlet is installed at a position higher in potential energy than the inlet.
  • the second feature is that the inlet valve 1 2 2 has a cantilever structure supported by the beam 1 2 4, and the beam 1 2 4 is arranged toward the inside of the liquid transfer chamber 1 2 1, and the opening of the inlet valve It was arranged so that the side was close to one side of the liquid transfer chamber 1 2 1. Another point is that the outlet port 123 is arranged close to the side opposite to the side where the inlet valve 122 is close. As a result, at the time of opening the inlet, the inlet valve 122 opens the side of the liquid supply chamber 121 most widest so that the liquid flows. The liquid that has flowed during priming and bombing becomes a smooth flow with a large curvature streamline 126 as shown by the arrow in Fig. 1 (b), preventing bubbles from remaining and Easily remove air bubbles.
  • the force in the case where the height of the liquid transfer chamber 1 29 is smaller than the height above the inlet 1 2 8 is smaller. If the threshold value h is not more than 0.02 mm, the liquid transfer chamber height 1 29 should be equal to the inlet upper height 1 28 so that bubbles are less likely to remain. In this embodiment, there are restrictions in forming the valve structure of the liquid device. Such a structure is more realistic.
  • FIG. 3 is a schematic diagram of a liquid feeding device according to a second embodiment of the present invention.
  • (a) is a cross-sectional view of the liquid sending device
  • (b) is a plan view of the liquid sending chamber.
  • the same reference numerals as in FIG. 1 indicate the same configuration as in the first embodiment.
  • the liquid transfer device is composed of three substrates, the same as in the first embodiment, a diaphragm substrate 110, an entrance / exit substrate 130, and a liquid transfer chamber substrate 220.
  • the difference from the first embodiment is that the projections 225 are formed in the liquid transfer chamber 221 of the liquid transfer chamber substrate 220.
  • the projections 2 25 are arranged in such a shape that the two surrounding flow paths are intermittently reduced in flow path area (a substantially hexagonal shape according to the shape of the liquid transfer chamber as shown in the figure).
  • the liquid transfer chamber height 2 29 is 500 ⁇ m
  • the height 2 27 (hereinafter referred to as the height above the protrusion) between the upper surface of the protrusion 222 and the diaphragm surface is 100 ⁇ m. 0 / zm.
  • the priming procedure of this liquid sending device is as follows. First, in order to replace the gas in the liquid sending chamber 1 2 1 of the liquid sending device with the liquid, a liquid introducing device for sending the liquid to be introduced into the suction port 1 34 of the liquid sending device is connected.
  • a liquid introduction device one of a syringe pump, a tube pump, and a gear pump may be used.
  • the pressurized liquid reaches the inlet valve 1 2 2 through the inlet port 1 3 3, and the pressurized pressure is applied. As a result, the inlet valve 1 2 2 is opened, and the liquid flows into the liquid supply chamber 1 2 1.
  • the inlet valve 122 supported by the beam 124 opens at the fulcrum at the root of the beam 124, so that the liquid passing through the inlet valve 122 flows from the adjacent side to the liquid transfer chamber 1 2 1 is satisfied and reaches the projection 2 25 on the inlet valve side. Then, the flow path above the projections 225 is filled with the liquid by the capillary action of the liquid. Here, since the end of the flow path on the projection is open, no bubbles remain.
  • the two flow paths on both sides of the projection Since the flow path is a speed-up flow in which the cross-sectional area of the flow path is continuously reduced, the flow path resistance gradually increases. Therefore, when the liquid advances in one of the flow paths around the protrusion in advance, the flow resistance of the other flow path becomes larger, so that the speed of the liquid in the preceding flow path decreases. descend. Then, when the flow path resistance difference becomes small, the traveling speed increases again, and reaches the outlet port 123 simultaneously from the two flow paths.
  • the priming operation may be performed using a suction pump as in the first embodiment.
  • the bombing operation is the same as in the first embodiment.
  • This embodiment has two features.
  • the first feature is that a protrusion 2225 is provided in the liquid transfer chamber with a liquid transfer chamber height of 229 m and a height of 220 m above the protrusion is set to 100 m.
  • the flow path on the protrusion allows the liquid to flow by capillary force, thereby preventing bubbles from remaining in the center of the liquid transfer chamber 121. Therefore, a liquid having better wettability than in the first embodiment has an effect of preventing bubbles from remaining. In order to quickly flow the liquid into the projection-shaped space, it is most effective to move the projections 2 25 to the inlet valve 122.
  • the second is that the two flow paths around the protrusion are flow paths for the accelerated flow.
  • the flow path resistance increases.
  • the traveling speed of the liquid decreases, and the traveling speed of the liquid in the other flow path increases. Therefore, the liquid proceeds in the two flow paths in a well-balanced manner, so that bubbles can be prevented from remaining in the liquid transfer chamber.
  • Bubbles are caused by static pressure drop.
  • This static pressure is reduced from the atmospheric pressure by the amount of decrease in the static pressure in the liquid sending chamber due to expansion of the volume of the liquid sending chamber and the static pressure This is a value obtained by subtracting the decrease. Since the location where the flow velocity becomes the highest when the liquid is sucked is the location where the cross-sectional area of the flow path is the smallest, that is, the static pressure becomes the lowest between the inlet valve and the inlet port, and the initiation of bubbles easily occurs. Therefore, a method for reducing the flow velocity between the inlet valve and the inlet port by optimizing the shape of the inlet valve or the inlet port is described below.
  • FIG. 4 is a schematic diagram of a liquid feeding device inlet valve according to a third embodiment of the present invention.
  • (a) and (b) are cross-sectional views of each liquid feeding device.
  • the same reference numerals as in FIG. 1 have the same configuration as in the first embodiment.
  • the liquid feeder shown in FIG. 4 (a) is composed of three substrates, the same as in the first embodiment, a diaphragm substrate 110, a liquid feed chamber substrate 120, and an entrance / exit substrate 4330. .
  • the difference from the first embodiment is that the diameter of the inlet port 43 3 of the inlet / outlet substrate 43 is larger than the diameter of the outlet port of the liquid transfer chamber substrate 120.
  • the cross-sectional area between the inlet valve 122 and the inlet port 43 is equal to the outlet valve 132 and the outlet port.
  • the flow rate of liquid between inlet valve 122 and inlet port 43 is equal to the flow rate of the outlet valve 132 and outlet port.
  • the flow rate of the liquid passing between the points 123 is smaller than that of the liquid, so that the initiation of bubbles is less likely to occur.
  • the liquid transfer apparatus shown in FIG. 4 (b) is composed of three substrates, the same as in the first embodiment, a diaphragm substrate 110, an inlet / outlet substrate 130 and a liquid transfer chamber substrate 420. Have been. The difference from the first embodiment is that a depression 4 2 6 is formed in the inlet valve 4 2 2 of the liquid transfer chamber substrate 4.
  • the cross-sectional area of the flow path between the inlet valve 422 and the artificial port 133 is determined by the outlet valve. No larger than the cross-sectional area of the flow path between 1 32 and outlet port 123 Therefore, the flow velocity of the liquid between the inlet valve 4 2 2 and the inlet port 13 3 is less than the flow velocity of the liquid between the outlet valve 1 3 2 and the outlet port 1 2 3 This makes it less likely that bubbles will begin to form.
  • the lift amount of the inlet valve 122 during the pumping is made larger than that of the outlet valve 132, and the inlet valve
  • the cross-sectional area of the passage between 122 and the inlet port 133 is larger than the cross-sectional area of the passage between the outlet valve 132 and the outlet port 123. Therefore, the flow velocity of the liquid passing between the inlet valve 122 and the inlet port 133 is smaller than the flow velocity of the liquid passing between the outlet valve 132 and the outlet port 123, and the air bubbles are generated. It is difficult for the first time to occur.
  • the flow rate of the liquid passing between the inlet valve and the inlet port is further reduced by making the lift amount of the inlet valve larger than the outlet valve at the time of pumping. It becomes bad. Thus, by reducing the flow velocity between the inlet valve and the inlet port, the initiation of bubbles is prevented.
  • FIG. 5 is an overall configuration diagram of an analyzer provided with the reagent supply mechanism of the present invention
  • FIG. 6 is a detailed explanatory diagram of the reagent supply mechanism of the present invention.
  • FIG. 5A shows the device 511 viewed from the front
  • FIG. 5B shows the device 511 viewed from the top.
  • a test tube 521 which contains a sample 52, and a sample holder 52, which holds the test tube 521, on the circumference.
  • a sample pipe 531 for sucking the sample 52 in the test tube 52 1 is provided beside the sample holder 52 2.
  • the sample pitcher 531 which sucks the sample and holds it inside, a three-dimensional drive mechanism 5333, which gives the nozzle 532 an up-and-down rotation, is shown in the figure. Not shown to aspirate sample into nozzle or eject sample from nozzle Pump is provided.
  • the sample holder 522 is driven to rotate by the rotary drive mechanism 523. At the other descent position of the nozzle of the sample c 531, the reaction vessel 541 moves while rotating sequentially.
  • the plurality of reaction vessels 541 are held on the circumference of the reaction disk 542.
  • the lower half of the reaction vessel 542 is connected to a constant temperature bath 543 through which constant temperature water flows.
  • the reaction disk 542 is supported by a reaction disk rotation drive mechanism 544 in order to sequentially move the reaction container to the lower position of the nozzle of the sample pitcher.
  • the first reagent supply section 5 51 and the second reagent supply section 5 61 1 A container cleaning mechanism 571 and a spectrometer 581 are provided.
  • the reagent supply section 55 1 is largely composed of four parts: a reagent container 55 2, a reagent holder 55 3, a liquid feeder 55 4 4, and a reagent holder 1 rotation drive mechanism 55 5 5.
  • Reagent holder 553 is a reagent container around central axis 556
  • a discharge port 563 is provided in the liquid sending device 554 vertically downward.
  • a magnetic unit 528 On the side surface of the reagent container 552, there is provided a magnetic unit 528 on which data describing the type of reagent is written.
  • a magnetic reader 581 for reading data of the magnetic section 582 is provided at a circumferential position corresponding to the reagent holder 553.
  • the signal line from the magnetic leader 581 is sent to the judgment section 557. It is connected.
  • the judging section 557 is connected to the liquid feeding apparatus control section 558.
  • the liquid sending device 554 is driven by a liquid sending device control section 558.
  • the reagent holder 55 3 is configured to be rotated by a reagent holder rotation drive mechanism 55 5.
  • FIG. 1 is a block diagram showing a configuration of an embodiment of a liquid sample analyzer according to the present invention.
  • the application example shown in the figure consists of a reagent ejection system, sample ejection system, reaction observation system, multi-wavelength photometer, input / output and arithmetic control system.
  • the reagent ejection system consists of a plurality (12 in the figure) of reagent containers 611 each containing a different reagent, and 12 systems connected to the bottom of each of the 12 reagent containers 6 1 1 A thin tube 6 1 2, and 12 reagent feeding units units 6 1 3, each of which is a reagent injecting means having a suction side connected to one of the 12 thin tubes 6 1 2, It comprises 12 reagent ejection pipes 6 14 connected to the ejection sides of two liquid sending devices 6 13.
  • the liquid supply unit 613 has a liquid supply unit 615 inside, and the reagent container 611 and the reagent discharge pit 613 are different from each other. Connected at Eve 6 16.
  • the sample ejection system consists of a sample container 6 21, a sample suction tube 6 2 2 with one end open and inserted into the sample container 6 2 1, and a sample injection with the suction side connected to the sample suction tube 6 2 2
  • the sample container 6 21, the sample aspirating tubing 6 2 2, the sample discharge pump 6 2 3, and the sample discharge pipet 6 24 are set on the moving mechanism 6 25 and the reactions are arranged in a line. Inject predetermined amount of sample into container 6 3 1 sequentially Has functions.
  • the number of specimen ejection systems is one, but a plurality of specimen ejection systems may be provided.
  • the reaction observation system is composed of the same number of reagent vessels 6 1 1 (1 2 in this example) as reaction vessels 6 3 1 arranged in a row, and a temperature holding mechanism 6 serving also as a support for the reaction vessels 6 3 1. 3 and a stirring mechanism 635 which is a stirring means also serving as a support for the temperature holding mechanism 632.
  • the wall of the reaction vessel 631 is made of a transparent material that allows measurement light to pass through.
  • the multi-wavelength photometer which is a reaction detecting means, includes a light source 641, which emits measurement light toward the reaction vessel 631, a lens 642 disposed on the optical axis of the measurement light, and the reaction vessel A concave diffraction grating 643 disposed at a position opposite to the lens 642 with the 631 interposed therebetween; and a concave diffraction grating 643 disposed at a position where measurement light reflected by the concave diffraction grating 643 is incident. And a photo diode array 644 for photoelectrically converting the measurement light. These are provided to a photometer moving mechanism (not shown) that reciprocates in parallel along the reaction vessel row so that the measurement light can be irradiated to all of the reaction vessels 631 arranged in a line. It is installed.
  • the input / output and arithmetic control system is a sequence controller for controlling the operation of the moving mechanism 6 25, the liquid feeding device 6 13 for injecting the reagent, the sample discharge pump 6 23, the multi-wavelength photometer and the photometer moving mechanism.
  • Controller 655 a signal processing unit 656 connected to the photo diode array 644, and a signal storage unit 6557 connected to the signal processing unit 6556.
  • An operation unit 658 as operation means connected to the signal storage unit 657; and a control unit connected to the operation unit 658 and the sequence controller 655 to control them.
  • one capillary tube, one pump, and one reagent discharge pipe are connected in series to one reagent container, which is different from the conventional device. Since different reagents do not flow in the same tube, there is no need for washing and no possibility of contamination. In addition, by reducing the size of the flow path system including the pump, the distance between the reagent container and the reaction container can be reduced, reducing the amount of reagent remaining in the tube, and wasting expensive reagents. It can be eliminated.
  • the analyzer shown in the figure is a micro TAS for testing specific items, and consists of a sample supply unit, reagent supply unit, reaction unit, and detection unit.
  • the sample supply section consists of a sample supply port 711 and a sample liquid sending device 712, and the sample injected from the sample supply port 711 reacts by a predetermined amount by the sample liquid sending device 712. Supply to room 7 3 1.
  • the reagent supply section is composed of a reagent chamber 7 2 1 and a reagent sending device 7 2 2, and the reagent stored in the reagent chamber 7 2 1 is supplied to the reaction chamber 7 2 2 by a predetermined amount in the reagent sending device 7 2 2. Feed 1 In this embodiment, two reagent supply units are provided to supply two types of reagents.
  • the reaction section includes a reaction chamber 731 and a stirring mechanism 732, and the sample and the reagent supplied to the reaction chamber 731 are mixed by the stirring mechanism 732. The reaction process between the sample and the reagent is monitored by the detection sensor 741 in the detection section. Each mechanism is connected by a flow path 7 13.
  • the present invention relates to a microphone port pump for accurately quantifying and sending a small amount of liquid, and in particular, to a structure of a liquid sending device configured to prevent generation of bubbles that affect the amount of liquid sent,
  • the liquid feeding device is applied to an analyzer.

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Abstract

L'invention porte sur un système de distribution de liquide à un débit allant de quelques νL à quelques centaines de νL par seconde utilisant une micropompe à membrane de quelques mm2, dans laquelle une soupape d'admission, opposant une faible résistance à l'entrée du liquide et une forte résistance à sa sortie, est couplée à une soupape d'échappement opposant une forte résistance à l'entrée du liquide et une faible résistance à sa sortie à l'air libre. Cette disposition permet d'éliminer les bulles d'air dans la chambre de distribution qui présente une surface croissant de l'entrée vers la sortie, et comporte une partie parallèle reliant l'entrée à la sortie, dont la surface décroît de l'entrée vers la sortie.
PCT/JP2000/001332 2000-03-06 2000-03-06 Systeme de distribution de liquide et dispositif associe Ceased WO2001066947A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2000/001332 WO2001066947A1 (fr) 2000-03-06 2000-03-06 Systeme de distribution de liquide et dispositif associe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2000/001332 WO2001066947A1 (fr) 2000-03-06 2000-03-06 Systeme de distribution de liquide et dispositif associe

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003166910A (ja) * 2001-11-30 2003-06-13 Asahi Kasei Corp 送液機構及び該送液機構を備える分析装置
JP2005001104A (ja) * 2003-06-10 2005-01-06 Samsung Electronics Co Ltd マイクロアクチュエータ及びこれを利用した流体移送装置
WO2005012729A1 (fr) * 2003-08-04 2005-02-10 Nec Corporation Pompe a membrane et systeme de refroidissement equipe d'une telle pompe a membrane
JP2010002229A (ja) * 2008-06-18 2010-01-07 Nippon Telegr & Teleph Corp <Ntt> フローセル
US8308453B2 (en) 2007-01-23 2012-11-13 Nec Corporation Diaphragm pump
US9914116B2 (en) 2015-09-10 2018-03-13 Panasonic Intellectual Property Management Co., Ltd. Microelement

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JPH0683954U (ja) * 1993-05-21 1994-12-02 テイケイ気化器株式会社 燃料ポンプ
JPH07151060A (ja) * 1993-11-29 1995-06-13 Tosoh Corp 圧電ポンプ
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JPH10159750A (ja) * 1996-11-26 1998-06-16 Jeol Ltd 定量送液ポンプ
JPH11247763A (ja) * 1998-03-04 1999-09-14 Hitachi Ltd 送液装置および自動分析装置

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JPH04502796A (ja) * 1989-11-10 1992-05-21 ウエストンブリッジ　インターナショナル　リミティド 起動を改善したマイクロポンプ
JPH0683954U (ja) * 1993-05-21 1994-12-02 テイケイ気化器株式会社 燃料ポンプ
JPH07151060A (ja) * 1993-11-29 1995-06-13 Tosoh Corp 圧電ポンプ
US5542821A (en) * 1995-06-28 1996-08-06 Basf Corporation Plate-type diaphragm pump and method of use
JPH10159750A (ja) * 1996-11-26 1998-06-16 Jeol Ltd 定量送液ポンプ
JPH11247763A (ja) * 1998-03-04 1999-09-14 Hitachi Ltd 送液装置および自動分析装置

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003166910A (ja) * 2001-11-30 2003-06-13 Asahi Kasei Corp 送液機構及び該送液機構を備える分析装置
JP2005001104A (ja) * 2003-06-10 2005-01-06 Samsung Electronics Co Ltd マイクロアクチュエータ及びこれを利用した流体移送装置
WO2005012729A1 (fr) * 2003-08-04 2005-02-10 Nec Corporation Pompe a membrane et systeme de refroidissement equipe d'une telle pompe a membrane
US8308453B2 (en) 2007-01-23 2012-11-13 Nec Corporation Diaphragm pump
JP2010002229A (ja) * 2008-06-18 2010-01-07 Nippon Telegr & Teleph Corp <Ntt> フローセル
US9914116B2 (en) 2015-09-10 2018-03-13 Panasonic Intellectual Property Management Co., Ltd. Microelement

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