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WO2001051651A3 - Expression et production elevees de s-adenosyl homocysteinase (sahh) recombinee a activite hautement specifique et dosages ameliores permettant d'obtenir de la s-adenosylmethionine (sam) - Google Patents

Expression et production elevees de s-adenosyl homocysteinase (sahh) recombinee a activite hautement specifique et dosages ameliores permettant d'obtenir de la s-adenosylmethionine (sam) Download PDF

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Publication number
WO2001051651A3
WO2001051651A3 PCT/US2001/001114 US0101114W WO0151651A3 WO 2001051651 A3 WO2001051651 A3 WO 2001051651A3 US 0101114 W US0101114 W US 0101114W WO 0151651 A3 WO0151651 A3 WO 0151651A3
Authority
WO
WIPO (PCT)
Prior art keywords
sahh
production
sam
adenosylmethionine
specific activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/001114
Other languages
English (en)
Other versions
WO2001051651A2 (fr
Inventor
Robert M Hoffman
Mingxu Xu
Qinghong Han
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anticancer Inc
Original Assignee
Anticancer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anticancer Inc filed Critical Anticancer Inc
Priority to CA2390057A priority Critical patent/CA2390057C/fr
Priority to AU2639701A priority patent/AU2639701A/xx
Priority to JP2001551225A priority patent/JP5058422B2/ja
Priority to DE60135632T priority patent/DE60135632D1/de
Priority to AU2001226397A priority patent/AU2001226397B2/en
Priority to EP01900999A priority patent/EP1250448B1/fr
Priority to KR1020027009060A priority patent/KR20020065925A/ko
Publication of WO2001051651A2 publication Critical patent/WO2001051651A2/fr
Publication of WO2001051651A3 publication Critical patent/WO2001051651A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne de nouvelles méthodes relatives à la détection et à la production de SAM ainsi qu'une activité enzymatique d'une nouvelle SAHH destinée à être utilisée dans ces méthodes. L'invention concerne également des méthodes supplémentaires, des compositions et des trousses relatives à cette nouvelle SAHH.
PCT/US2001/001114 2000-01-14 2001-01-12 Expression et production elevees de s-adenosyl homocysteinase (sahh) recombinee a activite hautement specifique et dosages ameliores permettant d'obtenir de la s-adenosylmethionine (sam) Ceased WO2001051651A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA2390057A CA2390057C (fr) 2000-01-14 2001-01-12 Expression et production elevees de s-adenosyl homocysteinase (sahh) recombinee a activite hautement specifique et dosages ameliores permettant d'obtenir de la s-adenosylmethionine (sam)
AU2639701A AU2639701A (en) 2000-01-14 2001-01-12 High expression and production of high-specific activity recombinant s-adenosyl homocysteinase (sahh) and improved assays for s-adenosylmethionine (sam)
JP2001551225A JP5058422B2 (ja) 2000-01-14 2001-01-12 高比活性組み換えｓ−アデノシルホモシステイナーゼ（ｓａｈｈ）の高発現および製造並びにｓ−アデノシルメチオニン（ｓａｍ）の改善されたアッセイ
DE60135632T DE60135632D1 (de) 2000-01-14 2001-01-12 Hochexpression und herstellung von recombinanter s-adenosyl homocysteinase mit hochspezifischer aktivität und verbesserte testverfahren für s-adenosylmethionine (sam)
AU2001226397A AU2001226397B2 (en) 2000-01-14 2001-01-12 High expression and production of high-specific activity recombinant S-adenosyl homocysteinase (SAHH) and improved assays for S-adenosylmethionine (SAM)
EP01900999A EP1250448B1 (fr) 2000-01-14 2001-01-12 Expression et production elevees de s-adenosyl homocysteinase (sahh) recombinee a activite hautement specifique et dosages ameliores permettant d'obtenir de la s-adenosylmethionine (sam)
KR1020027009060A KR20020065925A (ko) 2000-01-14 2001-01-12 고-특이적 활성 재조합 ｓ-아데노실호모시스테이나제(ｓａｈｈ)의 고발현과 생산 및ｓ-아데노실메티오닌(ｓａｍ)에 대한 개선된 분석법

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17644400P 2000-01-14 2000-01-14
US60/176,444 2000-01-14

Publications (2)

Publication Number Publication Date
WO2001051651A2 WO2001051651A2 (fr) 2001-07-19
WO2001051651A3 true WO2001051651A3 (fr) 2002-01-10

Family

ID=22644371

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/001114 Ceased WO2001051651A2 (fr) 2000-01-14 2001-01-12 Expression et production elevees de s-adenosyl homocysteinase (sahh) recombinee a activite hautement specifique et dosages ameliores permettant d'obtenir de la s-adenosylmethionine (sam)

Country Status (10)

Country Link
US (1) US6713273B2 (fr)
EP (2) EP1250448B1 (fr)
JP (1) JP5058422B2 (fr)
KR (1) KR20020065925A (fr)
CN (2) CN1416472A (fr)
AT (1) ATE407217T1 (fr)
AU (2) AU2001226397B2 (fr)
CA (1) CA2390057C (fr)
DE (1) DE60135632D1 (fr)
WO (1) WO2001051651A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9365625B1 (en) 2011-03-31 2016-06-14 David Gordon Bermudes Bacterial methionine analogue and methionine synthesis inhibitor anticancer, antiinfective and coronary heart disease protective microcins and methods of treatment therewith

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7774492B2 (en) * 2001-07-26 2010-08-10 Citrix Systems, Inc. System, method and computer program product to maximize server throughput while avoiding server overload by controlling the rate of establishing server-side net work connections
GB2394770A (en) * 2002-10-10 2004-05-05 Axis Shield Asa Assay for s-adenosyl methionine (SAM) and s-adenosyl homocysteine (SAH)
WO2005070014A2 (fr) * 2004-01-23 2005-08-04 Anticancer, Inc. Dosage de methionine enzymatique
US7504234B2 (en) * 2006-03-24 2009-03-17 The Regents Of The University Of Michigan Assays for S-adenosylmethionine (AdoMet)-dependent methyltransferase activity
KR100883530B1 (ko) * 2007-07-30 2009-02-13 한국과학기술연구원 대장암 진단용 단백질 마커 아데노실호모시스테이나제 및이에 대한 항체를 포함하는 대장암 진단키트
CN103710324B (zh) * 2013-12-09 2018-02-09 中国科学院微生物研究所 利用半胱氨酸水解酶家族成员制备(‑)γ内酰胺的方法
CN114544826B (zh) * 2020-11-24 2023-12-08 重庆医科大学 检测血浆中组氨酸的试剂在制备抑郁症检测试剂盒中的用途
CN117045661B (zh) * 2023-10-11 2024-01-30 广东医科大学附属医院 Sahh可逆型抑制剂的应用、药物组合物及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4148888A (en) * 1978-03-13 1979-04-10 The United States Of America As Represented By The Department Of Health, Education And Welfare 3-Deazaadenosine as an inhibitor of adenosylhomocysteine hydrolase with antiviral activity
US5998191A (en) * 1997-07-24 1999-12-07 Anticancer Inc. High specificity homocysteine assays for biological samples
WO2000063420A2 (fr) * 1999-04-16 2000-10-26 Anticancer, Inc. Procedes de dosage de fluides biologiques

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4148888A (en) * 1978-03-13 1979-04-10 The United States Of America As Represented By The Department Of Health, Education And Welfare 3-Deazaadenosine as an inhibitor of adenosylhomocysteine hydrolase with antiviral activity
US5998191A (en) * 1997-07-24 1999-12-07 Anticancer Inc. High specificity homocysteine assays for biological samples
WO2000063420A2 (fr) * 1999-04-16 2000-10-26 Anticancer, Inc. Procedes de dosage de fluides biologiques

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BAGNARA ALDO S ET AL: "Molecular characterisation of adenosylhomocysteinase from Trichomonas vaginalis.", MOLECULAR AND BIOCHEMICAL PARASITOLOGY, vol. 81, no. 1, 1996, pages 1 - 11, XP001013248, ISSN: 0166-6851 *
DATABASE EMBL 15 December 1995 (1995-12-15), XP002175637 *
DATABASE SWALL 1 October 1996 (1996-10-01), XP002175638 *
MINOTTO LINDA ET AL: "Trichomonas vaginalis: Expression and characterisation of recombinant S-adenosylhomocysteinase.", EXPERIMENTAL PARASITOLOGY, vol. 90, no. 2, October 1998 (1998-10-01), pages 175 - 180, XP001013283, ISSN: 0014-4894 *
SHIMIZU S ET AL: "OCCURRENCE OF S ADENOSYL HOMO CYSTEINE HYDROLASE EC-3.3.1.1 IN PRO KARYOTE CELLS CHARACTERIZATION OF THE ENZYME FROM ALCALIGENES-FAECALIS AND ROLE OF THE ENZYME IN THE ACTIVATED METHYL CYCLE", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 141, no. 2, 1984, pages 385 - 392, XP001013249, ISSN: 0014-2956 *
UELAND P M: "PHARMACOLOGICAL AND BIOCHEMICAL ASPECTS OF S ADENOSYL HOMO CYSTEINE AND S ADENOSYL HOMO CYSTEINE HYDROLASE", PHARMACOLOGICAL REVIEWS, vol. 34, no. 3, 1982, pages 223 - 254, XP001012612, ISSN: 0031-6997 *
YEO E-J ET AL: "INHIBITION OF GLYCINE N-METHYLTRANSFERASE BY 5-METHYLTETRAHYDROFOLATE PENTAGLUTAMATE", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 274, no. 53, 31 December 1999 (1999-12-31), pages 37559 - 37564, XP000938833, ISSN: 0021-9258 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9365625B1 (en) 2011-03-31 2016-06-14 David Gordon Bermudes Bacterial methionine analogue and methionine synthesis inhibitor anticancer, antiinfective and coronary heart disease protective microcins and methods of treatment therewith

Also Published As

Publication number Publication date
CN1416472A (zh) 2003-05-07
ATE407217T1 (de) 2008-09-15
EP1250448A2 (fr) 2002-10-23
JP2004508005A (ja) 2004-03-18
DE60135632D1 (de) 2008-10-16
KR20020065925A (ko) 2002-08-14
AU2639701A (en) 2001-07-24
US6713273B2 (en) 2004-03-30
EP1250448B1 (fr) 2008-09-03
EP1997508A2 (fr) 2008-12-03
US20020119491A1 (en) 2002-08-29
CA2390057C (fr) 2012-08-07
EP1997508B1 (fr) 2013-07-24
CA2390057A1 (fr) 2001-07-19
EP1997508A3 (fr) 2009-07-01
AU2001226397B2 (en) 2006-06-01
CN101298627A (zh) 2008-11-05
JP5058422B2 (ja) 2012-10-24
WO2001051651A2 (fr) 2001-07-19

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