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WO2001041931A3 - Multilayered microfluidic devices for analyte reactions - Google Patents

Multilayered microfluidic devices for analyte reactions Download PDF

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Publication number
WO2001041931A3
WO2001041931A3 PCT/US2000/033499 US0033499W WO0141931A3 WO 2001041931 A3 WO2001041931 A3 WO 2001041931A3 US 0033499 W US0033499 W US 0033499W WO 0141931 A3 WO0141931 A3 WO 0141931A3
Authority
WO
WIPO (PCT)
Prior art keywords
devices
microfluidic devices
multilayered microfluidic
fabricated
reactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/033499
Other languages
French (fr)
Other versions
WO2001041931A9 (en
WO2001041931A2 (en
Inventor
Cynthia G Briscoe
Jeremy W Burdon
Tony Chan
Barbara Foley
Piotr Grodzinski
George Hawkins
Rong-Fong Huang
Peter Kahn
Robert Marrero
Mark W Mcgarry
Todd Tuggle
Huinan Yu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Motorola Solutions Inc
Original Assignee
Motorola Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/460,281 external-priority patent/US6544734B1/en
Priority claimed from US09/458,534 external-priority patent/US6642046B1/en
Priority to CA002393690A priority Critical patent/CA2393690A1/en
Application filed by Motorola Inc filed Critical Motorola Inc
Priority to AU20827/01A priority patent/AU2082701A/en
Priority to EP00984156A priority patent/EP1237655A2/en
Priority to US10/149,318 priority patent/US20040043479A1/en
Priority to JP2001543266A priority patent/JP2003517591A/en
Publication of WO2001041931A2 publication Critical patent/WO2001041931A2/en
Publication of WO2001041931A3 publication Critical patent/WO2001041931A3/en
Publication of WO2001041931A9 publication Critical patent/WO2001041931A9/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6069—Construction of the column body with compartments or bed substructure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00781—Aspects relating to microreactors
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00891—Feeding or evacuation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00905—Separation
    • B01J2219/00916—Separation by chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28F—DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
    • F28F2260/00—Heat exchangers or heat exchange elements having special size, e.g. microstructures
    • F28F2260/02—Heat exchangers or heat exchange elements having special size, e.g. microstructures having microchannels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N2030/0095—Separation specially adapted for use outside laboratory, e.g. field sampling, portable equipments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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    • G01N2030/025—Gas chromatography
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    • G01N30/52—Physical parameters
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    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"
    • G—PHYSICS
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    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Nanotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices. In preferred aspects, the devices are fabricated using ceramic multilayer technology to form devices in which parallel, independently controlled molecular reactions, such as nucleic acid amplification reactions including the polymerase chain reaction (PCR) can be performed. Additionally, the devices can include and comprise micro-gas chromatographs similarly fabricated from ceramics.
PCT/US2000/033499 1999-12-09 2000-12-11 Multilayered microfluidic devices for analyte reactions Ceased WO2001041931A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2001543266A JP2003517591A (en) 1999-12-09 2000-12-11 Multilayer microfluidic device for reaction of analytical samples
US10/149,318 US20040043479A1 (en) 2000-12-11 2000-12-11 Multilayerd microfluidic devices for analyte reactions
CA002393690A CA2393690A1 (en) 1999-12-09 2000-12-11 Multilayered microfluidic devices for analyte reactions
AU20827/01A AU2082701A (en) 1999-12-09 2000-12-11 Multilayered microfluidic devices for analyte reactions
EP00984156A EP1237655A2 (en) 1999-12-09 2000-12-11 Multilayered microfluidic devices for analyte reactions

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US09/460,281 US6544734B1 (en) 1998-10-09 1999-12-09 Multilayered microfluidic DNA analysis system and method
US09/460,281 1999-12-09
US09/458,534 1999-12-09
US09/460,283 1999-12-09
US09/460,283 US6527890B1 (en) 1998-10-09 1999-12-09 Multilayered ceramic micro-gas chromatograph and method for making the same
US09/458,534 US6642046B1 (en) 1999-12-09 1999-12-09 Method and apparatus for performing biological reactions on a substrate surface
US46632599A 1999-12-17 1999-12-17
US09/466,325 1999-12-17

Publications (3)

Publication Number Publication Date
WO2001041931A2 WO2001041931A2 (en) 2001-06-14
WO2001041931A3 true WO2001041931A3 (en) 2002-01-03
WO2001041931A9 WO2001041931A9 (en) 2002-05-30

Family

ID=27504051

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/033499 Ceased WO2001041931A2 (en) 1999-12-09 2000-12-11 Multilayered microfluidic devices for analyte reactions

Country Status (5)

Country Link
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JP (1) JP2003517591A (en)
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6955777B2 (en) 2003-01-07 2005-10-18 International Business Machines Corporation Method of forming a plate for dispensing chemicals
US7338760B2 (en) 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
CN106164651A (en) * 2014-01-29 2016-11-23 Bg研究有限公司 Apparatus and method for thermal cycle biochemical operations

Families Citing this family (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6572830B1 (en) 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
US6637463B1 (en) 1998-10-13 2003-10-28 Biomicro Systems, Inc. Multi-channel microfluidic system design with balanced fluid flow distribution
US6601613B2 (en) 1998-10-13 2003-08-05 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6591852B1 (en) 1998-10-13 2003-07-15 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
EP1329714A4 (en) * 2000-09-25 2008-11-19 Gl Sciences Inc METHOD AND DEVICE FOR COLLECTING AND CONCENTRATING A SAMPLE
US6787339B1 (en) 2000-10-02 2004-09-07 Motorola, Inc. Microfluidic devices having embedded metal conductors and methods of fabricating said devices
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
JP4148778B2 (en) 2001-03-09 2008-09-10 バイオミクロ システムズ インコーポレイティッド Microfluidic interface equipment with arrays
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
ATE290432T1 (en) * 2001-10-03 2005-03-15 Csem Ct Suisse Electronique COMBINED DEVICE FOR TREATING BIOLOGICAL MICROMATRICES
US20030138819A1 (en) * 2001-10-26 2003-07-24 Haiqing Gong Method for detecting disease
KR100459896B1 (en) * 2002-03-06 2004-12-04 삼성전자주식회사 Thermostatic control Method and apparatus for Driving a PCR(polymerize chain reaction) chip
KR100450818B1 (en) * 2002-03-09 2004-10-01 삼성전자주식회사 Multi chamber PCR chip
TW592830B (en) 2002-03-29 2004-06-21 Casio Computer Co Ltd Chemical reaction apparatus and power supply system
JP3891131B2 (en) 2002-03-29 2007-03-14 カシオ計算機株式会社 Chemical reaction apparatus and power supply system
AU2003238501A1 (en) * 2002-06-14 2003-12-31 Axaron Bioscience Ag Hybridization chamber
US20030235521A1 (en) * 2002-06-21 2003-12-25 Shea Laurence R. Array assay devices and methods of using the same
JP4380977B2 (en) * 2002-10-28 2009-12-09 東洋紡績株式会社 Flow cell for surface plasmon resonance measurement
CN1217188C (en) * 2002-12-05 2005-08-31 清华大学 Miniature gas chromatographic column, gas chromatographic system and method for analysizing composition in sample
AU2004243070B2 (en) * 2003-05-23 2010-04-15 Bio-Rad Laboratories, Inc. Localized temperature control for spatial arrays of reaction media
US7648835B2 (en) 2003-06-06 2010-01-19 Micronics, Inc. System and method for heating, cooling and heat cycling on microfluidic device
KR101203402B1 (en) 2003-06-06 2012-11-23 마이크로닉스 인코포레이티드. System and method for heating, cooling and heat cycling on microfluidic device
JP2005024316A (en) * 2003-06-30 2005-01-27 Kyocera Corp Microchemical chip and manufacturing method thereof
WO2005011867A2 (en) 2003-07-31 2005-02-10 Handylab, Inc. Processing particle-containing samples
US7341834B2 (en) 2003-12-15 2008-03-11 Geneohn Sciences, Inc. Multiplexed electrochemical detection system and method
GB2416030B (en) * 2004-01-28 2008-07-23 Norchip As A diagnostic system for carrying out a nucleic acid sequence amplification and detection process
US7530259B2 (en) * 2004-02-17 2009-05-12 California Institute Of Technology On-chip temperature controlled liquid chromatography methods and devices
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7887750B2 (en) * 2004-05-05 2011-02-15 Bayer Healthcare Llc Analytical systems, devices, and cartridges therefor
US20090191096A1 (en) * 2004-07-29 2009-07-30 Kyocera Corporation Microchemical Chip
US7645423B2 (en) 2004-08-20 2010-01-12 International Business Machines Corporation Optical micro plugs for multichannel and multilayer pharmaceutical device
JP4574369B2 (en) * 2004-08-26 2010-11-04 京セラ株式会社 Wiring board and manufacturing method thereof
US7402616B2 (en) 2004-09-30 2008-07-22 Lifescan, Inc. Fusible conductive ink for use in manufacturing microfluidic analytical systems
JP2006133220A (en) * 2004-10-04 2006-05-25 Fuji Photo Film Co Ltd Sample supply method and sample supply device
US20090042280A1 (en) * 2004-12-13 2009-02-12 Geneohm Sciences, Inc., Fluidic cartridges for electrochemical detection of dna
DE102005004075B4 (en) * 2005-01-28 2008-04-03 Umicore Ag & Co. Kg Ceramic microreactor
JP2006223126A (en) * 2005-02-15 2006-08-31 Shimadzu Corp Reaction vessel, genetic polymorphism detection method and apparatus, and diagnostic method and apparatus
JP4714475B2 (en) * 2005-02-15 2011-06-29 株式会社島津製作所 Reaction vessel, gene polymorphism detection method and apparatus
EP1887363A4 (en) * 2005-04-01 2012-08-22 Konica Minolta Med & Graphic Micro overall analysis system, inspection chip, and inspection method
JP4870946B2 (en) * 2005-06-17 2012-02-08 凸版印刷株式会社 Reaction vessel and reaction method
JP4917765B2 (en) * 2005-06-17 2012-04-18 凸版印刷株式会社 PCR reaction vessel
DE102005029809B4 (en) * 2005-06-27 2007-04-26 Siemens Ag Apparatus and method for preparing a sample for analysis and apparatus and method for analyzing a sample
DE102005031920B4 (en) * 2005-07-07 2007-12-20 Isotopen Technologien München AG Apparatus and method for producing a small amount of a radioactive compound
JP2007064793A (en) * 2005-08-31 2007-03-15 Thk Co Ltd Solution supply device to dna microarray chip and solution supply method
US8075851B2 (en) 2005-09-29 2011-12-13 Siemens Medical Solutions Usa, Inc. Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography
JP2007132704A (en) * 2005-11-08 2007-05-31 Tosoh Quartz Corp Microchip base plate made of glass, its manufacturing method and microchip
JP2007189962A (en) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd Reaction vessel
JP2007189964A (en) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd Inspection board
JP2007189960A (en) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd Reaction vessel
JP5009532B2 (en) * 2006-01-20 2012-08-22 凸版印刷株式会社 Insulated container and reaction container unit
US7740806B2 (en) * 2006-02-28 2010-06-22 International Business Machines Corporation Ceramic microarray spotting device for bioassay printing
EP3088083B1 (en) 2006-03-24 2018-08-01 Handylab, Inc. Method of performing pcr with a mult-ilane cartridge
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
AU2007254191B2 (en) * 2006-05-16 2013-01-10 Fluidigm Corporation PCR-free sample preparation and detection systems for high speed biologic analysis and identification
GB0610432D0 (en) * 2006-05-26 2006-07-05 B G Res Ltd Performance issues in use of vessels for biological applications
US8173071B2 (en) 2006-08-29 2012-05-08 International Business Machines Corporation Micro-fluidic test apparatus and method
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008091694A2 (en) 2007-01-23 2008-07-31 Siemens Medical Solutions Usa, Inc. Fully-automated microfluidic system for the synthesis of radiolabeled biomarkers for positron emission tomography
JP2008180677A (en) 2007-01-26 2008-08-07 Konica Minolta Medical & Graphic Inc Microchip inspection system, microchip inspection apparatus, and program
US20080241844A1 (en) * 2007-02-06 2008-10-02 Network Biosystems, Inc. Devices and Methods for the Performance of Miniaturized In Vitro Assays
JP2008263959A (en) * 2007-03-23 2008-11-06 Toshiba Corp Nucleic acid detection cassette and nucleic acid detection apparatus
JP5557733B2 (en) 2007-04-04 2014-07-23 ネットバイオ・インコーポレーテッド Plastic microfluidic separation and detection platform
US8071035B2 (en) 2007-04-12 2011-12-06 Siemens Medical Solutions Usa, Inc. Microfluidic radiosynthesis system for positron emission tomography biomarkers
EP2171460B1 (en) 2007-07-13 2017-08-30 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
JP5852781B2 (en) 2007-07-31 2016-02-03 マイクロニクス， インコーポレイテッド Hygienic swab collection system, microfluidic assay device and method for diagnostic assays
GB0715171D0 (en) 2007-08-03 2007-09-12 Enigma Diagnostics Ltd Sample processor
GB0715170D0 (en) 2007-08-03 2007-09-12 Enigma Diagnostics Ltd Reaction vessel
EP2185929A4 (en) * 2007-08-09 2015-04-29 Univ Arizona DETECTION AND IDENTIFICATION OF BIOLOGICAL SAMPLES USING MICROFLUIDIC DEVICES
CL2008003008A1 (en) * 2007-10-12 2009-10-02 Bigtec Private Ltd A portable micro polymerase chain reaction (pcr) device based on a low temperature co-firing ceramic (ltcc) micro chip comprising reaction chamber, heater, heater temperature control, communication interface optical detection, and the method of monitoring and controlling it.
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
EP2401398B1 (en) * 2009-02-27 2017-02-08 Koninklijke Philips N.V. Genomic selection and sequencing using encoded microcarriers
JP2010213649A (en) * 2009-03-18 2010-09-30 Seiko Epson Corp Reaction vessel and reaction method for biological specimen
US9114395B2 (en) 2009-04-15 2015-08-25 Koninklijke Philips N.V. Cartridge for an amplification process
EP2443254A2 (en) 2009-06-15 2012-04-25 NetBio, Inc. Improved methods for forensic dna quantitation
JP5306134B2 (en) * 2009-09-28 2013-10-02 京セラ株式会社 Liquid feeding device
US8685749B2 (en) * 2009-12-02 2014-04-01 Whatman International Limited Methods and systems for processing samples on porous substrates
WO2012070431A1 (en) * 2010-11-22 2012-05-31 国立大学法人北海道大学 Portable liquid chromatograph and liquid chromatography
AU2012242510B2 (en) 2011-04-15 2015-05-14 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
KR101982627B1 (en) 2011-05-12 2019-05-27 에이엔디이 코포레이션 Method and compositions for rapid multiplex amplification of str loci
JP5945541B2 (en) * 2011-08-04 2016-07-05 株式会社堀場エステック Plate-type capillary column, capillary column unit and chromatograph using them
RU2622432C2 (en) 2011-09-30 2017-06-15 Бектон, Дикинсон Энд Компани Unified strip for reagents
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
CN107881219B (en) 2012-02-03 2021-09-10 贝克顿·迪金森公司 External file for molecular diagnostic test assignment and compatibility determination between tests
WO2014127804A1 (en) * 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Pathogen diagnosis system
WO2014127806A1 (en) * 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Dna amplifying kit
WO2014127805A1 (en) * 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Diagnosis method of pathogen
CA2936617C (en) * 2014-01-17 2022-11-29 Coastal Genomics Inc. Cassettes for use in automated parallel electrophoretic assays and methods for manufacturing and using same
US11285478B2 (en) 2016-04-04 2022-03-29 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
US9845499B2 (en) * 2016-04-04 2017-12-19 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
KR102422907B1 (en) 2016-11-17 2022-07-21 콤비네티 인코포레이티드 Methods and systems for nucleic acid analysis and quantification
CN109298734A (en) * 2018-09-07 2019-02-01 上海出入境检验检疫局工业品与原材料检测技术中心 The control method of intelligent temperature control detection device
CN113423503A (en) 2018-12-10 2021-09-21 康比纳提公司 Microfluidic array for sample digitization
CN109736778B (en) * 2019-03-01 2023-11-21 中国石油大学(北京) Wellbore hydration deformation infrared monitoring device and method
CN113000079B (en) * 2020-06-02 2023-09-22 山东大学 A heavy metal ion detection electrochemical microfluidic sensor chip and its preparation method
US12220698B2 (en) * 2020-06-30 2025-02-11 Samsung Electronics Co., Ltd. Microfluidic chip, and apparatus and method for detecting biomolecules
JP2023543054A (en) 2020-09-28 2023-10-12 コンビナティ インコーポレイテッド Devices and methods for sample processing
JPWO2023032883A1 (en) * 2021-08-30 2023-03-09
EP4524228A1 (en) * 2022-05-26 2025-03-19 Kikkoman Corporation Method and device for detecting microorganisms

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
EP0870541A2 (en) * 1997-04-11 1998-10-14 Eastman Kodak Company Integrated ceramic micro-chemical plant
WO1998050154A1 (en) * 1997-05-08 1998-11-12 University Of Minnesota Integrated microchip genetic testing system
US5939312A (en) * 1995-05-24 1999-08-17 Biometra Biomedizinische Analytik Gmbh Miniaturized multi-chamber thermocycler

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939312A (en) * 1995-05-24 1999-08-17 Biometra Biomedizinische Analytik Gmbh Miniaturized multi-chamber thermocycler
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
EP0870541A2 (en) * 1997-04-11 1998-10-14 Eastman Kodak Company Integrated ceramic micro-chemical plant
WO1998050154A1 (en) * 1997-05-08 1998-11-12 University Of Minnesota Integrated microchip genetic testing system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7338760B2 (en) 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
US6955777B2 (en) 2003-01-07 2005-10-18 International Business Machines Corporation Method of forming a plate for dispensing chemicals
CN106164651A (en) * 2014-01-29 2016-11-23 Bg研究有限公司 Apparatus and method for thermal cycle biochemical operations

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