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WO2000029624A3 - Methods and compositions for detection of nucleic acid sequences and signal amplification - Google Patents

Methods and compositions for detection of nucleic acid sequences and signal amplification Download PDF

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Publication number
WO2000029624A3
WO2000029624A3 PCT/US1999/027525 US9927525W WO0029624A3 WO 2000029624 A3 WO2000029624 A3 WO 2000029624A3 US 9927525 W US9927525 W US 9927525W WO 0029624 A3 WO0029624 A3 WO 0029624A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
detection
nucleic acid
acid sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/027525
Other languages
French (fr)
Other versions
WO2000029624A2 (en
Inventor
Elliot R Ramberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cygene Inc
Original Assignee
Cygene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cygene Inc filed Critical Cygene Inc
Priority to AU18245/00A priority Critical patent/AU1824500A/en
Priority to EP99961726A priority patent/EP1135525A2/en
Publication of WO2000029624A2 publication Critical patent/WO2000029624A2/en
Publication of WO2000029624A3 publication Critical patent/WO2000029624A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention comprises compositions and methods directed to analysis and detection of target nucleic acid sequences. Particularly, the methods and compositions comprise formation of duplex and triplex structures. In some embodiments, hairpin structures are employed. Novel restriction methods are provided for restriction of nucleic acids. Signal amplification systems, including duplex, triplex and MTRF systems, are also provided that allow for detection of very low copy number targets. These compositions and methods are especially useful in diagnostic assays.
PCT/US1999/027525 1998-11-19 1999-11-19 Methods and compositions for detection of nucleic acid sequences and signal amplification Ceased WO2000029624A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU18245/00A AU1824500A (en) 1998-11-19 1999-11-19 Methods and compositions for detection of nucleic acid sequences and signal amplification
EP99961726A EP1135525A2 (en) 1998-11-19 1999-11-19 Methods and compositions for detection of nucleic acid sequences and signal amplification

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10908298P 1998-11-19 1998-11-19
US60/109,082 1998-11-19
US11738999P 1999-01-27 1999-01-27
US60/117,389 1999-01-27
US13297699P 1999-05-07 1999-05-07
US60/132,976 1999-05-07

Publications (2)

Publication Number Publication Date
WO2000029624A2 WO2000029624A2 (en) 2000-05-25
WO2000029624A3 true WO2000029624A3 (en) 2000-10-05

Family

ID=27380598

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/027525 Ceased WO2000029624A2 (en) 1998-11-19 1999-11-19 Methods and compositions for detection of nucleic acid sequences and signal amplification

Country Status (3)

Country Link
EP (1) EP1135525A2 (en)
AU (1) AU1824500A (en)
WO (1) WO2000029624A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2003029441A1 (en) * 2001-09-28 2005-01-13 三光純薬株式会社 DNA chip signal amplification method
WO2003100099A1 (en) * 2002-05-24 2003-12-04 Cygene, Inc. Parallel stranded duplexes of deoxyribonucleic acid and methods of use
US9156986B2 (en) 2003-04-03 2015-10-13 Enzo Life Sciences, Inc. Multisignal labeling reagents and processes and uses therefor
US9696298B2 (en) 2003-04-03 2017-07-04 Enzo Life Sciences, Inc. Multisignal reagents for labeling analytes
US7514551B2 (en) 2003-04-03 2009-04-07 Enzo Life Sciences, Inc. Multisignal labeling reagents, and processes and uses therefor
CA3101473A1 (en) * 2018-06-07 2019-12-12 Amgen Inc. Detection assay for protein-polynucleotide conjugates
CN109115737B (en) * 2018-07-27 2021-03-26 青岛大学 Three-helix pH biosensor and application thereof
EP4148143A1 (en) * 2021-09-13 2023-03-15 Consejo Superior De Investigaciones Científicas (CSIC) Polypurine reverse hoogsteen hairpins and parallel clamps and their use as biosensors

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997015691A1 (en) * 1995-10-27 1997-05-01 Ramberg Elliot R Methods and compositions for detection of specific nucleotide sequences
EP0857791A1 (en) * 1996-12-21 1998-08-12 Roche Diagnostics GmbH Method of analysis using signal amplification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997015691A1 (en) * 1995-10-27 1997-05-01 Ramberg Elliot R Methods and compositions for detection of specific nucleotide sequences
EP0857791A1 (en) * 1996-12-21 1998-08-12 Roche Diagnostics GmbH Method of analysis using signal amplification

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
OLIVAS W M ET AL: "ANALYSIS OF DUPLEX DNA BY TRIPLE HELIX FORMATION: APPLICATION TO DETECTION OF A P53 MICRODELETION", BIOTECHNIQUES,US,EATON PUBLISHING, NATICK, vol. 16, no. 1, 1 January 1994 (1994-01-01), pages 128 - 132, XP000420685, ISSN: 0736-6205 *
POVSIC T J ET AL: "TRIPLE HELIX FORMATION BY OLIGONUCLEOTIDES ON DNA EXTENDED TO THE PHYSIOLOGICAL PH RANGE", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,US,AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, vol. 111, 1 January 1989 (1989-01-01), pages 3059 - 3061, XP000775708, ISSN: 0002-7863 *

Also Published As

Publication number Publication date
AU1824500A (en) 2000-06-05
WO2000029624A2 (en) 2000-05-25
EP1135525A2 (en) 2001-09-26

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