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WO2000016061B1 - Method for diagnosing the active stage of a cytomegalovirus infection in human substrates - Google Patents

Method for diagnosing the active stage of a cytomegalovirus infection in human substrates

Info

Publication number
WO2000016061B1
WO2000016061B1 PCT/RU1999/000246 RU9900246W WO0016061B1 WO 2000016061 B1 WO2000016061 B1 WO 2000016061B1 RU 9900246 W RU9900246 W RU 9900246W WO 0016061 B1 WO0016061 B1 WO 0016061B1
Authority
WO
WIPO (PCT)
Prior art keywords
cytomegalovirus
active stage
infection
diagnosing
anτigena
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/RU1999/000246
Other languages
French (fr)
Russian (ru)
Other versions
WO2000016061A2 (en
WO2000016061A3 (en
Inventor
Anatoly Jurievich Zvonarev
Igor Zakvanovich Zaitsev
Tengiz Dmitrievi Shalamberidze
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UNITARNOE GOSUDARSTVENNOE MOSKOVSKOE PREDPRIYATIE PO PROIZVODSTVU BAKTERIINYKH PREPARATOV MINISTERSTVA ZDRAVOOKHRANENIYA ROSSIISKOT FEDERATSII
UNITARNOE G MO PREDPR PROIZV B
Original Assignee
UNITARNOE GOSUDARSTVENNOE MOSKOVSKOE PREDPRIYATIE PO PROIZVODSTVU BAKTERIINYKH PREPARATOV MINISTERSTVA ZDRAVOOKHRANENIYA ROSSIISKOT FEDERATSII
UNITARNOE G MO PREDPR PROIZV B
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UNITARNOE GOSUDARSTVENNOE MOSKOVSKOE PREDPRIYATIE PO PROIZVODSTVU BAKTERIINYKH PREPARATOV MINISTERSTVA ZDRAVOOKHRANENIYA ROSSIISKOT FEDERATSII, UNITARNOE G MO PREDPR PROIZV B filed Critical UNITARNOE GOSUDARSTVENNOE MOSKOVSKOE PREDPRIYATIE PO PROIZVODSTVU BAKTERIINYKH PREPARATOV MINISTERSTVA ZDRAVOOKHRANENIYA ROSSIISKOT FEDERATSII
Priority to AU11918/00A priority Critical patent/AU1191800A/en
Publication of WO2000016061A2 publication Critical patent/WO2000016061A2/en
Publication of WO2000016061A3 publication Critical patent/WO2000016061A3/en
Publication of WO2000016061B1 publication Critical patent/WO2000016061B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56994—Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention pertains to the field of virology and relates to an express diagnosis for viral infections. This invention essentially relates to a method for diagnosing the active stage of cytomegalovirus infection in a patient, wherein said method involves carrying out a sorption of the specific antigen in solid phase, adding the serum to be analysed, carrying out an incubation using a conjugate of immunologic ferments as well as a substrate and estimating the results. The antigen consists of the recombinant, main, precursor and non-structured protein of the cytomegalovirus, and the active stage of the infection is detected upon the occurrence of antibodies against said cytomegalovirus.

Claims

00/16061 ΡСΤ/ΚΙΙ99/00246ИЗΜΕΗЁΗΗΑЯ ΦΟΡΜУЛΑ ИЗΟБΡΕΤΕΗИЯ[ποлучена Μеждунаροдным бюρο 15 φевρаля 2000 (15.02.00); πеρвοначальнο πеρвοначальнο заявленные πунκτы 1-2 φορмулы изοбρеτения заменены изменёнными πунκτами 1-2 (1 сτρаница)] 00/16061 ΡСΤ / ΚΙΙ99 / 00246 ΜΕΗΟΡΜΜΕΗΑ ΟΡΜΟΡΜΑΑ ΑΟΟ [[received by the International Bureau on 15 February 2000 (02.15.00); Paragraphs 1-2 of the original claims are replaced by the modified paragraphs 1-2 (1 page)] 1. Сποсοб οπρеделения аκτивнοй сτадии циτοмегалοвиρуснοй инφеκции в челοве- чесκиχ субсτρаτаχ , вκлючающий сορбцию сπециφичесκοгο анτигена на τвеρдую φазу, внесение исπыτуемοй сывοροτκи, инκубацию с иммунοφеρменτным κοнъ- югаτοм и субсτρаτοм с ποследующей οценκοй ρезульτаτοв, οτличающийся τем, чτο в κачесτве анτигена исποльзуюτ ρеκοмбинанτный οснοвнοй πρедρанний не- сτρуκτуρный белοκ циτοмегалοвйρуса, и πρи выявлении анτиτел κ нему деτеκ- τиρуюτ аκτивную сτадию инφеκции.1. Sποsοb οπρedeleniya aκτivnοy sτadii tsiτοmegalοviρusnοy inφeκtsii in chelοve- chesκiχ subsτρaτaχ, vκlyuchayuschy sορbtsiyu sπetsiφichesκοgο anτigena on τveρduyu φazu, making isπyτuemοy syvοροτκi, inκubatsiyu with immunοφeρmenτnym κοn- yugaτοm and subsτρaτοm with ποsleduyuschey οtsenκοy ρezulτaτοv, οτlichayuschiysya τem, chτο in κachesτve anτigena isποlzuyuτ ρeκοmbinanτny οsnοvnοy πρedρanny inadequate protein of cytomegalovirus, and in case of detection of antibodies to it, they deactivate an active stage of infection. 2. Сποсοб πο π.1, οτличающийся τем, чτο исποльзуюτ ρеκοмбинанτный οснοвнοй πρедρанний несτρуκτуρный белοκ циτοмегалοвиρуса челοвеκа, κлοниροванный и эκсπρессиροванный в белοκ-синτезиρующей сисτеме Ε.сοН.2. The method of π.1, which is distinguished by the fact that it uses a commercially available non-regular white non-obese and whitewashed white person. ИЗΜΕΗЁΗΗЫЙ ЛИСΤ (СΤΑΤЬЯ 19) LIQUID FOX (SΤJA 19)
PCT/RU1999/000246 1998-09-16 1999-07-22 Method for diagnosing the active stage of a cytomegalovirus infection in human substrates Ceased WO2000016061A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU11918/00A AU1191800A (en) 1998-09-16 1999-07-22 Method for diagnosing the active stage of a cytomegalovirus infection in a person

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU98117062 1998-09-16
RU98117062A RU2133472C1 (en) 1998-09-16 1998-09-16 Method for diagnosing active stage of human cytomegalovirus infection

Publications (3)

Publication Number Publication Date
WO2000016061A2 WO2000016061A2 (en) 2000-03-23
WO2000016061A3 WO2000016061A3 (en) 2000-06-02
WO2000016061B1 true WO2000016061B1 (en) 2000-07-06

Family

ID=20210421

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU1999/000246 Ceased WO2000016061A2 (en) 1998-09-16 1999-07-22 Method for diagnosing the active stage of a cytomegalovirus infection in human substrates

Country Status (3)

Country Link
AU (1) AU1191800A (en)
RU (1) RU2133472C1 (en)
WO (1) WO2000016061A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0700133D0 (en) 2007-01-04 2007-02-14 Humabs Llc Human cytomegalovirus neutralising antibodies and use thereof
US7947274B2 (en) 2007-01-04 2011-05-24 Humabs, LLC. Human cytomegalovirus neutralising antibodies and use thereof
JP5422649B2 (en) 2008-07-16 2014-02-19 インスティテュート フォー リサーチ イン バイオメディシン Human cytomegalovirus neutralizing antibody and use thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3619902A1 (en) * 1986-06-13 1988-03-03 Biotechnolog Forschung Gmbh CMV DNA STRUCTURES, EXPRESSION VECTOR DAFUER, CMV PROTEIN STRUCTURES AND THEIR USE
ZA878031B (en) * 1986-11-04 1988-04-28 Scripps Clinic And Research Foundation Nucleic acid probes for the detection of latent human cytomegalovirus in blood products
US5180813A (en) * 1989-03-24 1993-01-19 University Of Iowa Research Foundation Early envelope glycoprotein of human cytomegalovirus (hmcv) and monoclonal antibodies to the glycoproteins
RU2028156C1 (en) * 1991-02-15 1995-02-09 Научно-исследовательский институт вирусных препаратов РАМН Method of immunosorbent preparing for diagnosis of cytomegaloviral infection

Also Published As

Publication number Publication date
WO2000016061A2 (en) 2000-03-23
WO2000016061A3 (en) 2000-06-02
RU2133472C1 (en) 1999-07-20
AU1191800A (en) 2000-04-03

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