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WO2000008131A3 - Retrovirus pseudotypes et lignees cellulaires stables destinees a leur production - Google Patents

Retrovirus pseudotypes et lignees cellulaires stables destinees a leur production Download PDF

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Publication number
WO2000008131A3
WO2000008131A3 PCT/US1999/017702 US9917702W WO0008131A3 WO 2000008131 A3 WO2000008131 A3 WO 2000008131A3 US 9917702 W US9917702 W US 9917702W WO 0008131 A3 WO0008131 A3 WO 0008131A3
Authority
WO
WIPO (PCT)
Prior art keywords
retroviruses
pseudotyped
cells
glycoproteins
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/017702
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English (en)
Other versions
WO2000008131A8 (fr
WO2000008131A9 (fr
WO2000008131A2 (fr
Inventor
David A Sanders
Richard John Kuhn
Scott A Jeffers
Curtis Matthew Sharkey
Cynthia Lin North
Michael A Fischbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Purdue Research Foundation
Original Assignee
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation filed Critical Purdue Research Foundation
Priority to US09/762,224 priority Critical patent/US7033595B1/en
Priority to AU54658/99A priority patent/AU5465899A/en
Publication of WO2000008131A2 publication Critical patent/WO2000008131A2/fr
Publication of WO2000008131A3 publication Critical patent/WO2000008131A3/fr
Publication of WO2000008131A9 publication Critical patent/WO2000008131A9/fr
Publication of WO2000008131A8 publication Critical patent/WO2000008131A8/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13061—Methods of inactivation or attenuation
    • C12N2740/13062—Methods of inactivation or attenuation by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/14011—Filoviridae
    • C12N2760/14111—Ebolavirus, e.g. Zaire ebolavirus
    • C12N2760/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des cellules produisant de nouveaux rétrovirus pseudotypés possédant un large spectre d'activité. Dans un aspect de l'invention, les cellules produisent des rétrovirus pseudotypés avec au moins deux glycoprotéines virales différentes, telles que des glycoprotéines togavirales. Dans d'autres modes de réalisation, les cellules produisent des rétrovirus pseudotypés avec des glycoprotéines filovirales. L'invention concerne également des méthodes de production des cellules décrites ci-dessus, ainsi que des rétrovirus pseudotypés ainsi produits. Dans d'autres modes de réalisation encore, l'invention concerne des méthodes de criblage d'agents efficaces pour le blocage d'une entrée virale dans une cellule, y compris un entrée filovirale ou une entrée de virus possédant au moins deux glycoprotéines virales différentes disposées dans leur bicouche lipidique, tels que des togavirus. L'invention concerne en outre des méthodes d'utilisation des rétrovirus pseudotypés selon l'invention pour introduire des séquences nucléotidiques dans des cellules cibles, ainsi que des trousses de formation des rétrovirus pseudotypés selon l'invention.
PCT/US1999/017702 1998-08-04 1999-08-04 Retrovirus pseudotypes et lignees cellulaires stables destinees a leur production Ceased WO2000008131A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US09/762,224 US7033595B1 (en) 1998-08-04 1999-08-04 Pseudotyped retroviruses and stable cell lines for their production
AU54658/99A AU5465899A (en) 1998-08-04 1999-08-04 Pseudotyped retroviruses and stable cell lines for their production

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9524298P 1998-08-04 1998-08-04
US60/095,242 1998-08-04
US11240598P 1998-12-15 1998-12-15
US60/112,405 1998-12-15

Publications (4)

Publication Number Publication Date
WO2000008131A2 WO2000008131A2 (fr) 2000-02-17
WO2000008131A3 true WO2000008131A3 (fr) 2000-06-02
WO2000008131A9 WO2000008131A9 (fr) 2000-08-03
WO2000008131A8 WO2000008131A8 (fr) 2001-01-04

Family

ID=26789991

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/017702 Ceased WO2000008131A2 (fr) 1998-08-04 1999-08-04 Retrovirus pseudotypes et lignees cellulaires stables destinees a leur production

Country Status (2)

Country Link
AU (1) AU5465899A (fr)
WO (1) WO2000008131A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7981656B2 (en) 2002-06-04 2011-07-19 Purdue Research Foundation Pseudotyped retrovirus with modified ebola glycoprotein

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001083730A2 (fr) * 2000-04-28 2001-11-08 The Trustees Of The University Of Pennsylvania Vecteurs lentiviraux de recombinaison pseudotypes dans des enveloppes contenant des domaines de liaison de filovirus
WO2003035849A2 (fr) * 2001-10-26 2003-05-01 Purdue Research Foundation Procede de transfert de genes au moyen de lentivirus pseudotypes
BR112017004202A2 (pt) * 2014-09-03 2019-09-03 Bavarian Nordic As composições para intensificar respostas imunes e seus usos
HUE048318T2 (hu) 2014-09-03 2020-07-28 Bavarian Nordic As Módszerek és készítmények a filovírus fertõzés elleni védõ immunitás kialakítására
EP3484507A1 (fr) 2016-07-15 2019-05-22 Janssen Vaccines & Prevention B.V. Méthodes et compositions permettant l'induction d'une immunité protectrice contre l'infection par le virus de marburg
WO2018185732A1 (fr) 2017-04-06 2018-10-11 Janssen Vaccines & Prevention B.V. Régime de primo-vaccination/rappel par mva-bn et ad26.zebov ou ad26.filo

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4912030A (en) * 1985-01-15 1990-03-27 Institute Of Cancer Research Viral isolates and their use in diagnosis
US5185440A (en) * 1989-06-20 1993-02-09 North Carolina State University cDNA clone coding for Venezuelan equine encephalitis virus and attenuating mutations thereof
US5278056A (en) * 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
US5503974A (en) * 1989-06-30 1996-04-02 University Of California Retrovirus detection
US5512421A (en) * 1991-02-19 1996-04-30 The Regents Of The University Of California Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors
US5591624A (en) * 1988-03-21 1997-01-07 Chiron Viagene, Inc. Retroviral packaging cell lines
US5723287A (en) * 1992-09-22 1998-03-03 Medical Research Council Recombinant viruses displaying a nonviral polypeptide on their external surface
US5739018A (en) * 1996-08-07 1998-04-14 The Regents Of The University Of California Packaging cell lines for pseudotyped retroviral vectors

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4912030A (en) * 1985-01-15 1990-03-27 Institute Of Cancer Research Viral isolates and their use in diagnosis
US5278056A (en) * 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5591624A (en) * 1988-03-21 1997-01-07 Chiron Viagene, Inc. Retroviral packaging cell lines
US5185440A (en) * 1989-06-20 1993-02-09 North Carolina State University cDNA clone coding for Venezuelan equine encephalitis virus and attenuating mutations thereof
US5503974A (en) * 1989-06-30 1996-04-02 University Of California Retrovirus detection
US5512421A (en) * 1991-02-19 1996-04-30 The Regents Of The University Of California Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors
US5723287A (en) * 1992-09-22 1998-03-03 Medical Research Council Recombinant viruses displaying a nonviral polypeptide on their external surface
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
US5739018A (en) * 1996-08-07 1998-04-14 The Regents Of The University Of California Packaging cell lines for pseudotyped retroviral vectors

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KUHN R.J. ET AL.: "Chimeric Sindbis-Ross River Viruses to Study Interactions between Alphavirus Nonstructural and Structural Regions", J. VIROL.,, vol. 70, no. 11, November 1996 (1996-11-01), pages 7900 - 7909, XP002930976 *
LOPEZ S. ET AL.: "Nucleocapsid-Glycoprotein Interactions Required for Assembly of Alphaviruses", J. VIROL.,, vol. 68, no. 3, March 1994 (1994-03-01), pages 1316 - 1323, XP002930975 *
SANCHEZ ET AL.: "Sequence analysis of the Ebola virus genome: Organization, genetic elements and comparison with the genome of Marburg virus", VIRUS RESEARCH,, vol. 29, 1993, pages 215 - 240, XP002931007 *
WILL ET AL.: "Marburg virus gene 4 encodes the virion membrane protein, a type I transmembrane glycoprotein", J. VIROL.,, vol. 67, no. 3, March 1993 (1993-03-01), pages 1203 - 1210, XP002931008 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7981656B2 (en) 2002-06-04 2011-07-19 Purdue Research Foundation Pseudotyped retrovirus with modified ebola glycoprotein

Also Published As

Publication number Publication date
WO2000008131A8 (fr) 2001-01-04
WO2000008131A9 (fr) 2000-08-03
AU5465899A (en) 2000-02-28
WO2000008131A2 (fr) 2000-02-17

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