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WO2000008149A3 - Human neuronal acid-sensitive cationic channels, its cloning and applications - Google Patents

Human neuronal acid-sensitive cationic channels, its cloning and applications Download PDF

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Publication number
WO2000008149A3
WO2000008149A3 PCT/IB1999/001445 IB9901445W WO0008149A3 WO 2000008149 A3 WO2000008149 A3 WO 2000008149A3 IB 9901445 W IB9901445 W IB 9901445W WO 0008149 A3 WO0008149 A3 WO 0008149A3
Authority
WO
WIPO (PCT)
Prior art keywords
hasic3
inactivating
human
rdrasic
rasic3
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB1999/001445
Other languages
French (fr)
Other versions
WO2000008149A2 (en
Inventor
Rainer Waldmann
Frederic Bassilana
Michel Lazdunski
Weille Jan R De
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to CA2336221A priority Critical patent/CA2336221C/en
Priority to EP99936911A priority patent/EP1102844B1/en
Priority to AT99936911T priority patent/ATE449848T1/en
Priority to JP2000563774A priority patent/JP4489952B2/en
Priority to AU51879/99A priority patent/AU5187999A/en
Priority to DE69941709T priority patent/DE69941709D1/en
Publication of WO2000008149A2 publication Critical patent/WO2000008149A2/en
Publication of WO2000008149A3 publication Critical patent/WO2000008149A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Secondary Cells (AREA)

Abstract

Non-inactivating or slowly inactivating proton-gated cation channels are thought to play an important role in the perception of pain that accompanies tissue acidosis. We have identified a human proton-gated cation channel subunit that has biphasic desensitisationkinetics with both a rapidly inactivating Na+-selective and a sustained component. The protein shares 84 % sequence identity with the proton-gated cation channel rASIC3 (rDRASIC) from rat sensory neurones. The biphasic desnsitisation kinetics and the sequence homology suggest that this clone (hASIC3) is the human orthologue of rASIC3 (rDRASIC). While rASIC3 (rDRASIC) requires very acidic pH ( < pH 4.5) for activation of the sustained current, the non-inactivating hASIC3 current starts to be activated when the pH decreases to below pH 6. hASIC3 is an acid sensor and might play an important role in the detection of lasting pH changes in human. We localized the hASIC3 gene to the human chromosome 7q35, 6.4 cRad telomeric from the microsatellite AFMA082XC9.
PCT/IB1999/001445 1998-08-05 1999-08-05 Human neuronal acid-sensitive cationic channels, its cloning and applications Ceased WO2000008149A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA2336221A CA2336221C (en) 1998-08-05 1999-08-05 Human neuronal acid-sensitive cationic channels, its cloning and applications
EP99936911A EP1102844B1 (en) 1998-08-05 1999-08-05 Human neuronal acid-sensitive cationic channel, its cloning and applications
AT99936911T ATE449848T1 (en) 1998-08-05 1999-08-05 HUMAN NEURONAL ACID LABEL CATION CHANNEL, CLONING AND APPLICATIONS THEREOF
JP2000563774A JP4489952B2 (en) 1998-08-05 1999-08-05 Human neuronal acid-sensitive cation channel, its cloning and application
AU51879/99A AU5187999A (en) 1998-08-05 1999-08-05 Human neuronal acid-sensitive cationic channels, its cloning and applications
DE69941709T DE69941709D1 (en) 1998-08-05 1999-08-05 HUMAN NEURONAL ACID ELECTRICITY CATION CHANNEL, CLONING AND APPLICATIONS THEREOF

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9540898P 1998-08-05 1998-08-05
US60/095,408 1998-08-05

Publications (2)

Publication Number Publication Date
WO2000008149A2 WO2000008149A2 (en) 2000-02-17
WO2000008149A3 true WO2000008149A3 (en) 2000-04-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1999/001445 Ceased WO2000008149A2 (en) 1998-08-05 1999-08-05 Human neuronal acid-sensitive cationic channels, its cloning and applications

Country Status (9)

Country Link
US (1) US6287859B1 (en)
EP (1) EP1102844B1 (en)
JP (1) JP4489952B2 (en)
AT (1) ATE449848T1 (en)
AU (1) AU5187999A (en)
CA (1) CA2336221C (en)
DE (1) DE69941709D1 (en)
ES (1) ES2339412T3 (en)
WO (1) WO2000008149A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1183025B1 (en) 1999-05-19 2009-01-14 PainCeptor Pharma Corp. Inhibitors of proton-gated cation channels and their use in the treatment of ischaemic disorders
CA2304494A1 (en) * 2000-04-20 2001-10-20 Philippe Seguela A novel heteromultimeric ion channel receptor and uses thereof
US6635432B1 (en) 2000-04-25 2003-10-21 University Of Iowa Research Foundation Peptide potentiation of acid-sensory ion channel in pain
US10078087B2 (en) 2001-05-01 2018-09-18 Senomyx, Inc. Assays and enhancers of the human delta ENaC sodium channel
AU2003255953A1 (en) * 2002-08-12 2004-02-25 Pfizer Japan Inc. Human acid sensing ion channel 2b (hasic2b), process for producing the same, and its use
WO2004016167A1 (en) * 2002-08-16 2004-02-26 The General Hospital Corporation Non-invasive functional imaging of peripheral nervous system activation in humans and animals
CN1842709A (en) 2003-07-10 2006-10-04 塞诺米克斯公司 Improved electrophysiological assay using oocytes expressing human ENaC and use of PHENAMIL to increase ENaC enhancer efficacy in assays using membrane potential reporter dyes
US7598039B2 (en) * 2007-01-30 2009-10-06 Academia Sinica Treatment of insulin resistance
US9371383B2 (en) 2012-01-31 2016-06-21 Regeneron Pharmaceuticals, Inc. Anti-ASIC1 antibodies and uses thereof
CA2862332C (en) 2012-01-31 2021-05-18 Regeneron Pharmaceuticals, Inc. Anti-asic1 antibodies and uses thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2759373A1 (en) * 1997-02-11 1998-08-14 Centre Nat Rech Scient ACIDITE SENSITIVE NEURONAL NEURONAL CATIONIC CHANNEL, ITS CLONING AND ITS APPLICATIONS
WO1998054316A1 (en) * 1997-05-30 1998-12-03 Sanofi-Synthelabo Sodium channel receptor
WO1999011784A1 (en) * 1997-08-29 1999-03-11 University College London Proton gated ion channel proteins
WO1999021981A1 (en) * 1997-10-29 1999-05-06 Mcgill University Dna encoding a human proton-gated ion channel and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2759373A1 (en) * 1997-02-11 1998-08-14 Centre Nat Rech Scient ACIDITE SENSITIVE NEURONAL NEURONAL CATIONIC CHANNEL, ITS CLONING AND ITS APPLICATIONS
WO1998054316A1 (en) * 1997-05-30 1998-12-03 Sanofi-Synthelabo Sodium channel receptor
WO1999011784A1 (en) * 1997-08-29 1999-03-11 University College London Proton gated ion channel proteins
WO1999021981A1 (en) * 1997-10-29 1999-05-06 Mcgill University Dna encoding a human proton-gated ion channel and uses thereof

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BABINSKI, KAZIMIERZ ET AL: "Molecular cloning and regional distribution of a human proton receptor subunit with biphasic functional properties", J. NEUROCHEM. (1999), 72(1), 51-57, XP000856807 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002129617, Database accession no. AF057711 *
DE WEILLE J R ET AL: "Identification, functional expression and chromosomal localisation of a sustained human proton-gated cation channel.", FEBS LETTERS, (1998 AUG 21) 433 (3) 257-60., XP002129616 *
ERIC LINGUEGLIA ET AL.: "A modulatory subunit of acid sensing ion channels in brain and dorsal root ganglion cells", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 47, 21 November 1997 (1997-11-21), MD US, pages 29778 - 29783, XP002068544 *
FRÉDÉRIC BASSILANA ET AL.: "The acid-sensitive ionic channel subunit ASIC and the mammalian degenerin MDEG form a heteromultimeric H+-gated Na+ channel with novel properties", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 46, 14 November 1997 (1997-11-14), MD US, pages 28819 - 28822, XP002068543 *
ISHIBASHI K ET AL: "Molecular cloning of a DEG/ENaC sodium channel cDNA from human testis", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,US,ACADEMIC PRESS, SAN DIEGO, CA, vol. 245, no. 2, 1 April 1998 (1998-04-01), pages 589 - 593, XP002080466, ISSN: 0006-291X *
MARGARET P. PRICE ET AL.: "Cloning and expression of a novel human brain Na+ Channel", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 14, 5 April 1996 (1996-04-05), MD US, pages 7879 - 7882, XP002068541 *
RAINER WALDMANN ET AL.: "Molecular cloning of a non-inactivating proton-gated Na+ channel specific for sensory neurons", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 34, 22 August 1997 (1997-08-22), MD US, pages 20975 - 20978, XP002080467 *
RAINER WALDMANN ET AL.: "The mammalian degenerin MDEG, an amiloride-sensitive cation channel activated by mutations causing neurodegeneration in Caenorhabditis elegans", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 18, 3 May 1996 (1996-05-03), MD US, pages 10433 - 10436, XP002051361 *
REINER WALDMANN ET AL.: "A proton-gated cation channel involved in acid-sensing", NATURE, vol. 386, no. 6621, 13 March 1997 (1997-03-13), LONDON GB, pages 173 - 177, XP002068589 *

Also Published As

Publication number Publication date
EP1102844A2 (en) 2001-05-30
US6287859B1 (en) 2001-09-11
CA2336221A1 (en) 2000-02-17
ES2339412T3 (en) 2010-05-19
JP2002524042A (en) 2002-08-06
AU5187999A (en) 2000-02-28
WO2000008149A2 (en) 2000-02-17
ATE449848T1 (en) 2009-12-15
CA2336221C (en) 2010-06-01
DE69941709D1 (en) 2010-01-07
EP1102844B1 (en) 2009-11-25
JP4489952B2 (en) 2010-06-23

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