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WO2000070058A3 - Plant cellulose synthase genes - Google Patents

Plant cellulose synthase genes Download PDF

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Publication number
WO2000070058A3
WO2000070058A3 PCT/GB2000/001890 GB0001890W WO0070058A3 WO 2000070058 A3 WO2000070058 A3 WO 2000070058A3 GB 0001890 W GB0001890 W GB 0001890W WO 0070058 A3 WO0070058 A3 WO 0070058A3
Authority
WO
WIPO (PCT)
Prior art keywords
cellulose synthase
synthase genes
plant cellulose
cell walls
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/001890
Other languages
French (fr)
Other versions
WO2000070058A2 (en
Inventor
Simon Turner
Neil Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Manchester
Original Assignee
Victoria University of Manchester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Victoria University of Manchester filed Critical Victoria University of Manchester
Priority to AU49345/00A priority Critical patent/AU4934500A/en
Publication of WO2000070058A2 publication Critical patent/WO2000070058A2/en
Publication of WO2000070058A3 publication Critical patent/WO2000070058A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1059—Cellulose synthases (2.4.1.12; 2.4.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to an isolated nucleic acid molecule comprising a cellulose synthase gene specifically expressed during deposition of secondary cell walls in lignin containing cells and the use of such a gene or its promoter to modulate the expression of enzymes involved in the synthesis of plant cell walls, to produce transgenic plants.
PCT/GB2000/001890 1999-05-18 2000-05-17 Plant cellulose synthase genes Ceased WO2000070058A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU49345/00A AU4934500A (en) 1999-05-18 2000-05-17 Plant cellulose synthase genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9911379.7A GB9911379D0 (en) 1999-05-18 1999-05-18 Plant cellulose synthase genes
GB9911379.7 1999-05-18

Publications (2)

Publication Number Publication Date
WO2000070058A2 WO2000070058A2 (en) 2000-11-23
WO2000070058A3 true WO2000070058A3 (en) 2001-04-05

Family

ID=10853572

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/001890 Ceased WO2000070058A2 (en) 1999-05-18 2000-05-17 Plant cellulose synthase genes

Country Status (3)

Country Link
AU (1) AU4934500A (en)
GB (1) GB9911379D0 (en)
WO (1) WO2000070058A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6867352B2 (en) 1998-07-14 2005-03-15 E. I. Du Pont De Nemours And Company Plant cellulose synthases
EP1057891A1 (en) * 1999-06-02 2000-12-06 Centrum Voor Plantenveredelings- En Reproduktieonderzoek Use of the BNM3 transcriptional activator to control plant embryogenesis and regeneration processes
SE0301233D0 (en) * 2003-04-28 2003-04-28 Swetree Technologies Ab Tissue specific promoters
JP2006526990A (en) 2003-06-06 2006-11-30 アーバージェン リミテッド ライアビリティ カンパニー Compositions and methods for modulating plant cell polysaccharides
US7238512B2 (en) 2003-10-17 2007-07-03 E. I. Du Pont De Nemours And Company Method to produce para-hydroxybenzoic acid in the stem tissue of green plants by using a tissue-specific promoter
BRPI0400246A (en) * 2004-01-22 2006-03-21 Fundacao De Estudos Agrarios L gene expression cassette, use of one or more gene expression cassettes, method of genetic transformation in plant cells, method of obtaining genetically modified plants, genetically modified plants, plant use, derived plants, genetically modified seeds, use of genetically modified seed, wood, wood use, cellulose, cellulose use, paper, paper use and method of modulating the level of polypeptides in plants
BR112016008008A2 (en) * 2013-10-21 2017-10-17 Swetree Technologies Ab transgenic trees having reduced xylan content
CN106399358A (en) * 2016-06-03 2017-02-15 华南农业大学 Application of lotus cellulose synthase gene NnuCESA4
CN118547000B (en) * 2024-07-10 2026-02-17 西南大学 Application of over-expressed PtoCC gene in improving poplar biomass

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998000549A1 (en) * 1996-06-27 1998-01-08 The Australian National University MANIPULATION OF CELLULOSE AND/OR β-1,4-GLUCAN
WO1998018949A2 (en) * 1996-10-29 1998-05-07 Calgene Llc Plant cellulose synthase and promoter sequences
EP0875575A2 (en) * 1997-04-01 1998-11-04 Nisshinbo Industries, Inc. Cellulose synthase gene
WO2000004166A2 (en) * 1998-07-14 2000-01-27 E.I. Du Pont De Nemours And Company Plant cellulose synthases

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998000549A1 (en) * 1996-06-27 1998-01-08 The Australian National University MANIPULATION OF CELLULOSE AND/OR β-1,4-GLUCAN
WO1998018949A2 (en) * 1996-10-29 1998-05-07 Calgene Llc Plant cellulose synthase and promoter sequences
EP0875575A2 (en) * 1997-04-01 1998-11-04 Nisshinbo Industries, Inc. Cellulose synthase gene
WO2000004166A2 (en) * 1998-07-14 2000-01-27 E.I. Du Pont De Nemours And Company Plant cellulose synthases

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE EBI 5 November 1997 (1997-11-05), NEWMAN T. ET AL.: "Arabidopsis EST", XP002153882 *
REITER W -D: "ARABIDOPSIS THALIANA AS A MODEL SYSTEM TO STUDY SYNTHESIS, STRUCTURE, AND FUNCTION OF THE PLANT CELL WALL", PLANT PHYSIOLOGY AND BIOCHEMISTRY,FR,GAUTHIER-VILLARS, PARIS, vol. 36, no. 1/02, January 1998 (1998-01-01), pages 167 - 176, XP000901529, ISSN: 0981-9428 *
STERKY FREDRIK ET AL: "Gene discovery in the wood-forming tissues of poplar: Analysis of 5,692 expressed sequence tags.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 95, no. 22, 27 October 1998 (1998-10-27), Oct. 27, 1998, pages 13330 - 13335, XP002153880, ISSN: 0027-8424 *
TAYLOR NEIL G ET AL: "The irregular xylem3 locus of Arabidopsis encodes a cellulose synthase required for secondary cell wall synthesis", PLANT CELL,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 11, no. 5, May 1999 (1999-05-01), pages 769 - 779, XP002134253, ISSN: 1040-4651 *
TURNER SIMON R ET AL: "Collapsed xylem phenotype of arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall.", PLANT CELL, vol. 9, no. 5, 1997, pages 689 - 701, XP002153881, ISSN: 1040-4651 *

Also Published As

Publication number Publication date
GB9911379D0 (en) 1999-07-14
WO2000070058A2 (en) 2000-11-23
AU4934500A (en) 2000-12-05

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