WO2000069452A1 - Remedies for cancer and infection with helicobacter pylori and process for producing the same - Google Patents

Abstract

Remedies for cancer and infection with Helicobacter pylori comprising a mixture of Scutellaria barbatae, Elfvingia applanta Karst., oldenlandia, coix seed, licorice root and anemarrhena rhizome which has been optionally extracted with hot water or ethyl alcohol.

Description

 TECHNICAL FIELD Therapeutic agents for cancer and Helicopaque-H. Pylori infection, and methods for producing the same

 The present invention relates to a remedy for precancerous lesions, tumor markers—abnormal hyperplasia, cancer, or helicopaque H. pylori infection, and a method for producing the same. Background technology

 Cancer is the leading or leading cause of death in Japan and Western Europe. In contrast, cancer treatment methods include drug therapy, surgery, and radiation.

 Of the pharmacotherapy, the types of cancer for which chemotherapy is effective are limited, and even for the types of cancer for which chemotherapy is effective, it is difficult to cause tumor regression for many terminal cancers, and However, if P53, which is a tumor suppressor gene of a patient, has a mutation or a defect, there is a problem that chemotherapy is not effective and treatment is difficult.

 Also, even if chemotherapy was effective, the side effects were often strong, and the treatment was difficult, and there was a problem that recurrence occurred even after showing efficacy.

 Furthermore, in recent years, it has been found that Helicobacter pylori infection causes stomach ulcers and even gastric cancer, but there is a problem that there is no better treatment than antibiotics. Disclosure of the invention

The present invention has been made in view of the above-mentioned conventional problems, and is not limited to normal cancer, but also terminal cancer in which chemotherapy is not effective, and mutations or deletions in p53, which is a tumor suppressor gene, Furthermore, a therapeutic agent that is effective against Helicobacter pylori infection, And a method for producing the same.

 As a result of research conducted by the inventor, the present invention has revealed that at least half of the six crude drugs, Hanedan, Umeparasitic, White-flowered Snake Tongue, Yakuinin, Licorice, and Chichimo It has been found that the mixture of mothers has an antitumor effect, has tumor regression and prolongs the survival or prolongs the survival of cancer patients. It is based on the fact that it has been found that it inhibits bacterial growth.

 The present invention provides a mixture of at least a half-flowered lotus, a ume parasitic, a licorice, and a mother of six kinds of herbal medicines, a half-flowered lotus, a ume-parasitic, a white flower snake tongue grass, a good linden, a licorice, and a mother-in-law. The proportion of the weight of each crude drug is 0.3% to 63% for Haneda lotus, 0.3% to 63% for ume infestation, 0.3% to 60% for white flower, and 0.3% for 60%, and good jin. 3% to 60%, Licorice is 0.3% to 60%, and Chimbo is 0.015% to 60%. Is to achieve.

 In the above-mentioned remedy for cancer and Helicobacter pylori infection, at least half of the six crude drugs of the above-mentioned six kinds of crude drugs, namely, Hanedori, Umeyose, Shirakawa snake tongue grass, Oyakuin, Licorice, Chichimo Is composed of one of the water-soluble components extracted from hot water extracted from parasitism, licorice and tomochi, and the alcohol-soluble component extracted from ethyl alcohol. 0.3% to 63%, ume infestation 0.3% to 63%, white flower snake tongue grass 0.3% to 60%, good bonito 0.3% to 60%, licorice 0.3% ~ 60%, Chimo is 0.015% ~ 60%.

Further, in the therapeutic agent for cancer and Helicobacter pylori infection, the crude extract of the water-soluble component or the alcohol-soluble component may be dried to form a powder. In the above-mentioned remedy for cancer and helicopak-pylori infection, in a crude drug state, the above-mentioned semi-lotus consists of whole plant of Scutellaria barbate, and the ume parasite is Polyporaceae (Polyporaceae) Consisting of the whole components of Elfvingia applantaKars, the white-flowered snake tongue grass is a member of the genus Hedgotis diffusa Willd. Ma-youn Staph) is made from the seeds of the seeds except the seed coat, and the licorice is Glycyrrhiza uraknsis

Fischer), and her mother may consist of rhizomes of Annemarrhena asaphodelodes Bunge.

 Further, in the remedy for cancer and helicopaque Yuichi pylori infection, 12 parts by weight of the half-branch lotus, 8 parts by weight of ume parasite, 8 parts by weight of white flower snake tongue grass, 8 parts by weight of good jin Based on 3 parts by weight of licorice and 3 parts by weight of Chichimo, at least half of lotus, lotus plum, licorice, and licorice, 300 to 500 parts by weight of water are used. Extract the water-soluble component with hot water for one minute, or extract the alcohol-soluble component with ethyl alcohol, and filter it through a filter to obtain a crude extract of about 150 to 250 parts by weight. You may do so.

 The invention of this production method is based on at least half-flowered lotus, ume-parasitic, licorice, and The above object is achieved by a method for producing a drug for treating cancer and helicopax-H. Pylori infection characterized by extracting one of a water-soluble component and an alcohol-soluble component with one of ethyl alcohol and ethyl alcohol. is there.

 The method for producing a remedy for cancer and Helicobacter pylori infection, comprising: at least a half-lotus, ume-parasitic, licorice of the half-branch, ume parasitism, white-flowered snake tongue grass, good-for-all, licorice, and chichimo. Alternatively, one of the water-soluble component and the alcohol-soluble component may be extracted from the mixture of Chimo using hot water or ethyl alcohol.

 Further, in the method for producing a drug for treating cancer and Helicobacter pylori infectious disease, the method for producing a remedy for infectious disease of Helicobacter pylori as described above comprises at least a half of lotus, plum One of a water-soluble component and an alcohol-soluble component may be separately extracted from parasitism, licorice and tomochi with hot water or ethyl alcohol, and the extracts may be mixed.

Further, in the method for producing a drug for treating cancer and Helicobacter pylori infection, Based on 12 parts by weight of the semi-branch lotus, 8 parts by weight of ume parasite, 8 parts by weight of white flower snake tongue grass, 8 parts by weight of good jin, 3 parts by weight of licorice, 3 parts by weight of Chichimo, Among them, at least half-branch lotus, ume parasite, licorice, and tomochi are extracted with 300 to 500 parts by weight of hot water for 30 to 60 minutes, or alcohol-soluble components are extracted with ethyl alcohol. It may be extracted and filtered through a filter to obtain about 150 to 250 parts by weight of a crude extract.

 Further, in the method for producing a drug for treating cancer and Helicobacter pylori infection, the crude extract of about 150 to 250 parts by weight is dried to obtain a powder of 2 to 5 parts by weight. It may be.

 Further, in the method for producing a therapeutic agent for treating cancer and Helicobacter pylori infection, the method comprises the steps of: One of the soluble component and the alcohol-soluble component may be extracted.

 Further, in the method for producing a therapeutic drug for treating cancer and Helicobacter pylori infection, in a crude drug state, the half-branch lotus comprises a whole plant of Scutellaria barbatae, and the ume parasite is Polyporaceae ) Consists of all the constituents of ElfVingia applantaKarst., White-snake snake tongue grass is composed of the whole plant of the moss family Hedgotis diffusaWilld. Ma-youn Staph) may be made of seeds without seed coat, licorice may be made of roots of Glycyrrhiza uralensis Fischer, and tomato may be made of rhizome of Annemarrhena asaphodelodes Bunge.

In the above-mentioned semi-ring lotus, which is made up of semi-ring lotus, ume parasite and licorice, semi-lotus, ume parasite and licorice have anti-tumor effect independently in vitro. In addition, Hanedaren-to has a low tumor growth inhibitory effect on gastric cancer cell line MKN45 with wi1 dtypep53 (cancer suppressor gene) and KAT0-ΠΙ which is a p53-deficient strain. Has a cell death-inducing effect at high concentrations. Lotus has the strongest tumor growth inhibitory and cell death-inducing effects, followed by ume parasite and licorice. The cell death induced at that time is partially The Sith, mostly necrotic.

 Hanedarento inhibited the growth of MKN45 most strongly. The second inhibitor was KATO-III, which inhibited the proliferation of normal human dermal fibroblast cell lines the least. However, as a disadvantage of Haneda Rento, it was found that the antitumor effect against p53-deficient strain was low. Therefore, a method to enhance the antitumor effect against p53-deficient strain is needed.

 The inventor has studied a method for enhancing the antitumor effect. It was found that Michimo had MKN45 and KATO-III cell growth inhibitory and cell death-inducing effects. In this case, the cell death is almost 100% apoptosis. Furthermore, compared to MKN45, she showed a significant difference in the growth inhibitory effect and the apoptosis-inducing effect on KATO-III, a p53-deficient strain. Tomochi morphologically expressed apoptotic bodies in cancer cells. Extraction of fragmented DNA from the mother-to-mother-added cancer cells and agarose gel electrophoresis revealed DNA ladder, and she found that the mother-of-mother induced cancer cells to die by apoptosis. In addition, she found that it released cytochrome C from mitochondria of cancer cells, activated intracellular caspases, and induced apoptosis.

 Next, by adding Chimo to Hanedarento, the inhibitory effect of Hanedarento on tumor growth and inducing cell death was achieved without Chimochi antagonizing P53-deficient KATO-ΙΠ. We considered whether to increase. As a result, Chimo showed no antagonism to the antitumor effect of Hanedarento, but enhanced it. At that time, the combination of the concentrations of Hanedarento and Chimo was the most effective when the ratio was 4: 1 and 2: 1. When the combination of Hanedarento and Chimo is 4: 1, the mixture was found to have an antitumor effect on KATO-ΙΠ and MKN45 with the same level of growth inhibition. From the above, it was confirmed that the antitumor effect against p53-deficient or mutated cell lines was enhanced by mixing Hanedarento and Chimomo in a concentration ratio of 4: 1. Has found.

Chimo also has a synergistic antitumor effect against MKN 45, which has wi ldt pe p 53, and the combination of Hanedarento and Chimo's concentrations at that time is 8: 1. 4 to 1 worked most synergistically. From the above, we found that when Hanedayu and Chimo are combined, the most synergistic effect is basically 4 to 1. However, they also discovered that 8: 1 or 2: 1 is acceptable.

 As described above, Hanjangren, Chimbo, ume parasite, and licorice each directly suppress cancer growth and cause cell death of cancer, but licorice has a weak effect of inducing cancer cell death. However, the cancer cell death-inducing effect of licorice is enhanced by a synergistic or additive effect with other components. In addition, white flower snake tongue grass enhances NK activity.

 In addition, Haneda Lotus and Tomomo discovered that they inhibited the growth of Helicobacter pylori at inVitr0.

 From the above, the therapeutic agent of the present invention is effective for treating cancer, precancer, abnormally high tumor marker values, and Helicobacter pylori infection.

BEST MODE FOR CARRYING OUT THE INVENTION

 Hereinafter, embodiments of the present invention will be described in detail.

 First, 120 parts by weight of Haneda Lotus, 80 parts by weight of ume parasite, 80 parts by weight of white flower snake tongue grass, 80 parts by weight of coconut, 30 parts by weight of licorice, 30 parts by weight of Chimo 250 parts by weight of a crude extract obtained by extracting with hot water for 30-60 minutes with hot water for 30-60 minutes and filtering through a 1 mm filter or 50% by weight of freeze-dried powder Of the present invention as a therapeutic agent for cancer and helicopaque Yuichi pylori infection of the present invention.

 The above-mentioned half-branch lotus, ume parasite, white flower snake tongue plant, good nin, licorice, and tomochi are all crude drugs, and water-soluble components are extracted from each small piece with hot water.

 In a crude drug state, the half-branch lotus is composed of a whole plant of a half-branch lotus (Scutellaria barbatae). The tongue grass is Futabamugra

(Hedgotis diffusa Willd.) Consists of whole plants, and good seeds consist of seeds of coix lacryma-jobi and var. Ma-youn Staph without seed coat. Licorice is Kanzo カ ン (Glvcyrrhiza uralensis). Fischer) The roots of my mother, Hanasuge (Annemarrhena asaphodelodes Bunge).

 The average adult intake for the treatment of cancer is 80 ml of the crude extract or 600 to 500 Omg of the freeze-dried powder three times a day. If a stronger effect is expected, the crude extract may be taken in 2500 ml or its lyophilized powder up to 51 g once daily or in divided doses. It is possible to increase or decrease the amount of each crude drug in the above mixture of crude drugs. The weight of each crude drug per day before extraction is 0.1 to 50 g for half-branch lotus, 0.1 to 50 g for ume parasite, and 0.1 to 50 g for white flower snake tongue grass. It is possible to have a dosage of 0.1 to 50 g for jin, 0.1 to 30 g for licorice, and 0.05 to 50 g for Tomochi.

 In addition, the proportion of the weight of each crude drug ranges from 0.3% to 63% for Haneda lotus and 0.

From 3% to 63%, white flower snake tongue grass from 0.3% to 60%, good kin.

A mixture containing 3% to 6060%, licorice from 0.3% to 60%, and Chichimo from 0.015% to 60% is possible.

 The basic composition of the crude drug is half-branch lotus, ume parasite, white-flowered snake tongue grass, good-living seed, licorice, and tomochi. Absent. The basic formula is as follows: 12 g of half-branch lotus, 8 g of ume parasite, 8 g of white flower snake tongue grass, 8 g of good-looking jin, 3 g of licorice, 3 g of chinboshi It can be provided in a customized form.

 Also, as mentioned above, 120 parts by weight of semi-branch lotus, 80 parts by weight of ume parasite, 80 parts by weight of white flower snake tongue grass, 80 parts by weight of good liquor, 30 parts by weight of licorice, 30 parts by weight of Chichimo are 5,000 parts The crude extract was extracted with hot water for 30-60 minutes and filtered through a 1 mm filter.

Although 100 parts by weight are obtained, the alcohol extraction component may be extracted with ethyl alcohol. Alternatively, it may be dried or freeze-dried powder. It is also possible to pack the dried or freeze-dried powder into capsules, mix it with compatible non-influencing substances, or to make tablets by mechanical pressure. Usually, the water-soluble component extracted from hot water obtained from the above-mentioned half-branch lotus, plum infestation, white flower snake tongue grass, saccharin, licorice, and mother-to-child or ethyl alcohol-extracted component is orally administered. The component or one component may be administered enema, intraperitoneally, pleurally, or intravenously.

 Conditions or diseases that can be treated are cancer, precancerous, tumor marker-abnormal high value, Helicobacter pylori infection, and helicopacu-H. Pylori infection has adversely affected the disease state. (Muc osa as soci at ed l ymph oidtisue) One of 1 ymp homa.

 Examples of administration of the therapeutic agent for cancer and helicopa-pylori infection of the present invention are as follows. In the case of cancer treatment, the crude extract is dried or freeze-dried powder to treat cancer and helicobacter pylori infection. Basically, the crude extract is taken at a dose of 600 to 500 Omg or the equivalent thereof 1 to 4 times a day until the cancer is reduced, regressed, or has a growth inhibitory effect. You can have a few rest days without taking it. If the effect is low, the powder can be added in a timely manner. If ethyl alcohol is extracted, the dose may be reduced, but in that case, about 75% of the dose should be administered. If the growth inhibitory effect appears, take it at intervals. If the tumor has regressed, continue for about a year.

For treatment of Helicobacter pylori infection, the crude extract is dried or freeze-dried into a powdered drug for treating cancer and helicopa-pylori infection 600 to 5000 mg, or the crude extract corresponding thereto. Is to take 1 to 4 times a day for 7 consecutive days. If helicopter H. pylori eradicates quickly, the dose can be reduced to one day. If eradication is required for a long time, it can be extended to one month. If ethyl alcohol is extracted, the dose may be reduced, but about 75% of the dose should be administered. If the growth inhibitory effect is observed, take it for each period. If symptoms of recurrence appear, resume taking the drug again. Industrial applicability

 The therapeutic agent of the present invention is useful not only for cancers for which chemotherapy is effective, but also for terminal cancers where chemotherapy is not effective, mutations or deletions in p53, which is a tumor suppressor gene, and also for Helicobacter pylori. It is also effective against bacterial infections.

Claims

The scope of the claims  1. Six kinds of herbal medicines of half-branch, ume-parasitic, white flower snake tongue grass, good jin, licorice, and chinbo The ratio of crude drug weight is 0.3% to 63% for Hanren Lotus, 0.3% to 63% for ume parasitism, 0.3% to 60% for white flower snake tongue grass, and 0.3 for good jin. % Of licorice, 0.3% to 60% of licorice, and 0.015% to 60% of licorice.  2. Hot water extracted from at least half of the six crude drugs of Haneda, Umeparasitic, White flower snake tongue grass, Oyakuin, Licorice, and Chichimo It is composed of one of a soluble component and an alcohol-soluble component extracted with ethyl alcohol.The ratio of the weight of each crude drug before extraction is 0.3% to 63% for Haneda lotus and 0.3 for ume parasitic. % ~ 6 3%, white flower snake tongue grass 0.3% ~ 60%, good bonito 0.3% ~ 60%, licorice 0.3% ~ 60%, chichimo 0.03% A remedy for cancer and Helicobacter pylori infection characterized by being と し た 60%.  3. The therapeutic drug for cancer and helicopaque Yuichi pylori infection according to claim 2, wherein the crude extract of the water-soluble component or the alcohol-soluble component is dried to form a powder.  4. The herbicide according to any one of claims 1 to 3, wherein the half-branch lotus is a whole plant of a half-branch lotus (Scutellaria barbatae); (Polyporaceae) Consists of all the constituents of Elfvingia applantaKars. jobi L. var. ma-youn Staph) is made from seeds except for the seed coat. A therapeutic drug for cancer and helicopaque-virilitis infection, characterized by the following: 5. The method according to any one of claims 1 to 4, wherein the semi-branch lotus is 12 parts by weight, the ume parasite is 8 parts by weight, the white flower snake tongue plant is 8 parts by weight, the good nin is 8 parts by weight, and the licorice is 3 parts by weight. And Chimo are based on 3 parts by weight, and at least half of them are extracted with hot water for 30 to 60 minutes with 300 to 500 parts by weight of water. A remedy for cancer and helicopaque Yuichi pylori infection characterized by obtaining a crude extract of about 150 to 250 parts by weight by filtration through a filter.  6. Among the six crude drugs of half-flowering lotus, ume parasite, white flower snake tongue grass, oyster, licorice, and chichimo, from at least half-flowering lotus, ume parasitic, licorice, and chimochi, hot water and ethyl alcohol A method for producing a drug for treating cancer and Helicobacter pylori infection, comprising extracting one of a water-soluble component and an alcohol-soluble component.  7. The method according to claim 6, wherein at least half of the mixture of the half-branch lotus, the ume parasite, the white-flowered snake tongue grass, the sapling, the licorice, and the primrose, the mixture of hot water and A method for producing a remedy for cancer and Helicopaque-H. Pylori infection, comprising extracting one of a water-soluble component and an alcohol-soluble component with one of the ethyl alcohols. 8. The method according to claim 6, wherein at least half of lotus, ume parasitism, white flower snake tongue grass, oysters, licorice, and chinboshi are selected from: Production of a therapeutic drug for cancer and Helicopaque-H. Pylori infection characterized by extracting one of a water-soluble component and an alcohol-soluble component with one of the ethyl alcohols and mixing the extracts. Method.  9. The claim according to claim 6, 7 or 8, wherein the semi-branch lotus is 12 parts by weight, the ume parasite is 8 parts by weight, the white flower snake tongue grass is 8 parts by weight, the good nin is 8 parts by weight, and the licorice is 3 parts by weight. Based on 3 parts by weight of Chimomo, at least a half of lotus, ume parasite, licorice, and Chichimo are extracted with 300 to 500 parts by weight of hot water for 30 to 60 minutes. A method for producing a remedy for cancer and Helicobacter pylori infection, characterized by obtaining a crude extract of about 150-250 parts by weight by filtration through a filter. 10. The method according to claim 9, wherein the crude extract of about 150 to 250 parts by weight is dried. A method for producing a therapeutic drug for cancer and Helicobacter pylori infection, comprising obtaining 2 to 5 parts by weight of powder.  11. The method according to any one of claims 6 to 10, wherein the water-soluble component and the alcohol are obtained from small pieces of the half-branch lotus, the ume parasite, the white-flowered snake tongue grass, the well-being, the licorice, and the mother. A method for producing a drug for treating cancer and Helicobacter pylori infection, comprising extracting one of the soluble components.  12. The method according to any one of claims 6 to 11, wherein in a crude drug state, the half-branch lotus comprises a whole plant of a half-branch Lotus (Scutellaria barbatae), and (Polyporaceae ^ Consists of all the constituents of Elfvingia applantaKarst.). The white flower snake tongue grass is composed of the whole plant of the genus Hedgotis diffusaWilld. L. var. Ma-youn Staph) consists of seeds excluding the seed coat. Licorice consists of the roots of Glycyrrhiza uralensis Fischer, and her mother is the rhizome ge of Annemarrhena asaphodelodes Bunge. A method for producing a remedy for cancer and helicopaque-pylori infection, comprising a rhizome. Revised paper (Rule 91)