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WO2000068434A3 - Multiple tag analysis - Google Patents

Multiple tag analysis Download PDF

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Publication number
WO2000068434A3
WO2000068434A3 PCT/US2000/012391 US0012391W WO0068434A3 WO 2000068434 A3 WO2000068434 A3 WO 2000068434A3 US 0012391 W US0012391 W US 0012391W WO 0068434 A3 WO0068434 A3 WO 0068434A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
molecules
reporter molecules
decoding
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/012391
Other languages
French (fr)
Other versions
WO2000068434A2 (en
Inventor
Paul M Lizardi
Darin R Latimer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Priority to JP2000617205A priority Critical patent/JP2002543813A/en
Priority to CA002371843A priority patent/CA2371843A1/en
Priority to AU48248/00A priority patent/AU782408B2/en
Priority to EP00930427A priority patent/EP1196632A2/en
Publication of WO2000068434A2 publication Critical patent/WO2000068434A2/en
Anticipated expiration legal-status Critical
Publication of WO2000068434A3 publication Critical patent/WO2000068434A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Disclosed is a method of detecting multiple analytes in a sample in a single assay. The method is based on encoding target molecules with signals followed by decoding of the encoded signal. This encoding/decoding uncouples the detection of a target molecule from the chemical and physical properties of the target molecule. In basic form, the disclosed method involves association of one or more reporter molecules with one or more target samples, association of one or more decoding tags with the reporter molecules, and detection of the decoding tags. The reporter molecules associate with target molecules in the target sample(s). Generally, the reporter molecules correspond to one or more target molecules, and the decoding tags correspond to one or more reporter molecules. Thus, detection of particular decoding tags indicates the presence of the corresponding reporter molecules. In turn, the presence of particular reporter molecules indicates the presence of the corresponding target molecules. The sensitivity of the disclosed method can also be enhanced by including a signal amplification step prior to detection. Medical applications of this method include the analysis of the phenotypic status or replicative status of cells (growth or quiescence) and the assessment of normal and neoplastic cells in histologic or cytologic specimens in normal and disease states. For example, a pathologist may use the method to link a phenotypic state with the protein profile of lesion believed to contain malignant or pre-malignant cells.
PCT/US2000/012391 1999-05-07 2000-05-05 Multiple tag analysis Ceased WO2000068434A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000617205A JP2002543813A (en) 1999-05-07 2000-05-05 Multiple label analysis
CA002371843A CA2371843A1 (en) 1999-05-07 2000-05-05 Multiple tag analysis
AU48248/00A AU782408B2 (en) 1999-05-07 2000-05-05 Multiple tag analysis
EP00930427A EP1196632A2 (en) 1999-05-07 2000-05-05 Multiple tag analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13296999P 1999-05-07 1999-05-07
US60/132,969 1999-05-07

Publications (2)

Publication Number Publication Date
WO2000068434A2 WO2000068434A2 (en) 2000-11-16
WO2000068434A3 true WO2000068434A3 (en) 2002-01-31

Family

ID=22456431

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/012391 Ceased WO2000068434A2 (en) 1999-05-07 2000-05-05 Multiple tag analysis

Country Status (5)

Country Link
EP (1) EP1196632A2 (en)
JP (1) JP2002543813A (en)
AU (1) AU782408B2 (en)
CA (1) CA2371843A1 (en)
WO (1) WO2000068434A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7615625B2 (en) 1992-08-04 2009-11-10 Replicon, Inc. In vitro amplification of nucleic acid molecules via circular replicons

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001086296A2 (en) * 2000-05-05 2001-11-15 Agilix Corporation Highly multiplexed reporter carrier systems
US20020142345A1 (en) * 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
US7083951B2 (en) 2001-02-14 2006-08-01 The Regents Of The University Of California Flow cytometric detection method for DNA samples
CA2466861A1 (en) * 2001-11-06 2003-07-10 Agilix Corporation Sensitive coded detection systems
US7192703B2 (en) * 2003-02-14 2007-03-20 Intel Corporation, Inc. Biomolecule analysis by rolling circle amplification and SERS detection
EP2287602A1 (en) 2005-06-07 2011-02-23 Centre National de la Recherche Scientifique (CNRS) Use of ionic matrices for MALDI mass spectrometry analysis of tissue sections
US8741566B2 (en) 2005-07-01 2014-06-03 Dako Denmark A/S Immunohistochemistry and in situ hybridization detection method
US20100105145A1 (en) * 2005-07-01 2010-04-29 Dako Denmark A/S Method of simultaneously visualizing multiple biological targets
EP2414536B1 (en) * 2009-04-03 2014-06-18 Battelle Memorial Institute Fluorescence cued raman identification of viable organisms
GB0908633D0 (en) * 2009-05-20 2009-06-24 D3 Technologies Ltd Detection of target analytie by signal amplication
IN2012DN02337A (en) 2009-10-12 2015-08-21 Ventana Med Syst Inc
US10385392B2 (en) * 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
EP3036530B1 (en) * 2013-08-19 2020-03-25 University Of Houston Phosphorescent reporters
WO2015038784A1 (en) 2013-09-13 2015-03-19 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry
CA2943617C (en) 2014-04-02 2022-11-01 The Board Of Trustees Of The Leland Stanford Junior University An apparatus and method for sub-micrometer elemental image analysis by mass spectrometry
US10036066B2 (en) 2015-03-17 2018-07-31 Dako Denmark A/S In situ hybridization detection method
EP3274719B1 (en) 2015-03-25 2021-02-24 The Board of Trustees of the Leland Stanford Junior University Single cell analysis using secondary ion mass spectrometry
US9797879B2 (en) 2015-04-23 2017-10-24 The Board Of Trustees Of The Leland Stanford Junior University Method for multiplexed sample analysis by photoionizing secondary sputtered neutrals
KR20230166141A (en) 2016-12-09 2023-12-06 얼티뷰, 인크. Improved methods for multiplex imaging using labeled nucleic acid imaging agents
CN117551741A (en) 2017-03-31 2024-02-13 乌尔蒂维尤股份有限公司 DNA-antigen exchange and amplification

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002526A1 (en) * 1990-07-27 1992-02-20 Chiron Corporation Large comb-type branched polynucleotides
WO1992002528A1 (en) * 1990-07-27 1992-02-20 Chiron Corporation Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor
WO1995020320A1 (en) * 1994-01-31 1995-08-03 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US5614362A (en) * 1987-10-15 1997-03-25 Chiron Corporation Nucleic acid hybridization assay for hepatitis B virus DNA
WO1998004746A1 (en) * 1996-07-31 1998-02-05 Mount Sinai School Of Medicine Of The City University Of New York Nucleic acid amplification method: ramification-extension amplification method (ram)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203989B1 (en) * 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614362A (en) * 1987-10-15 1997-03-25 Chiron Corporation Nucleic acid hybridization assay for hepatitis B virus DNA
WO1992002526A1 (en) * 1990-07-27 1992-02-20 Chiron Corporation Large comb-type branched polynucleotides
WO1992002528A1 (en) * 1990-07-27 1992-02-20 Chiron Corporation Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor
WO1995020320A1 (en) * 1994-01-31 1995-08-03 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
WO1998004746A1 (en) * 1996-07-31 1998-02-05 Mount Sinai School Of Medicine Of The City University Of New York Nucleic acid amplification method: ramification-extension amplification method (ram)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7615625B2 (en) 1992-08-04 2009-11-10 Replicon, Inc. In vitro amplification of nucleic acid molecules via circular replicons

Also Published As

Publication number Publication date
AU4824800A (en) 2000-11-21
AU782408B2 (en) 2005-07-28
JP2002543813A (en) 2002-12-24
EP1196632A2 (en) 2002-04-17
WO2000068434A2 (en) 2000-11-16
CA2371843A1 (en) 2000-11-16

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