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WO2000065039A3 - Identification d'elements de controle d'adn reagissant a des stimuli specifiques - Google Patents

Identification d'elements de controle d'adn reagissant a des stimuli specifiques Download PDF

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Publication number
WO2000065039A3
WO2000065039A3 PCT/US2000/011460 US0011460W WO0065039A3 WO 2000065039 A3 WO2000065039 A3 WO 2000065039A3 US 0011460 W US0011460 W US 0011460W WO 0065039 A3 WO0065039 A3 WO 0065039A3
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sar
mutants
genes
expression
gene
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Ceased
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PCT/US2000/011460
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WO2000065039A2 (fr
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Klaus Maleck
Kay Ann Lawton
Robert Arthur Dietrich
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Novartis AG
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Novartis AG
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Publication of WO2000065039A2 publication Critical patent/WO2000065039A2/fr
Publication of WO2000065039A3 publication Critical patent/WO2000065039A3/fr
Anticipated expiration legal-status Critical
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/8239—Externally regulated expression systems pathogen inducible
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Health & Medical Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

Pour identifier les gènes dont les protéines interviennent dans la régulation de la résistance systémique acquise en aval de la mort cellulaire, on a utilisé un crible visant à isoler les mutants qui expriment cette résistance de manière constitutive. Le promoteur PR-1, qui est le marqueur le plus fiable pour une apparition de ladite résistance, a été cloné devant le gène rapporteur de luciférase de luciole (Photinus pyralis) et transformé en Arabidopsis. Une ligne transgénique à forme d'expression de luciférase parallèle au gène PR-1 endogène a été identifiée et soumise à une mutagenèse EMS pour isoler les mutants qui expriment de manière constitutive le gène de luciférase PR-1. Des techniques biochimiques, cytologiques, pathologiques et génétiques ont été employées pour caractériser plus avant les mutants et pour mettre en évidence l'isolation des mutants de la résistance systémique acquise en aval de la mort cellulaire. Cette caractérisation permet de faire la distinction entre les classes de mutants cim et de décrire les modifications physiologiques intervenant durant la phase de maintenance de la résistance systémique acquise. En outre, l'utilisation de puces à jeu ordonné de microéchantillons a permis d'examiner simultanément l'ensemble du génome végétal pour déceler les gènes dont l'expression se modifie en réponse à des facteurs biotiques ou abiotiques. La comparaison des modifications de l'expression génique sur différents traitements a permis d'identifier des groupes de gènes corégulés (régulons), et on a examiné les séquences génomiques de gènes dans un régulon pour identifier des motifs de séquences communes pouvant vraisemblablement tenir lieu d'éléments régulateurs. L'approche utilisée fait appel à une conception expérimentale fondée sur la biologie du système à l'étude, en combinaison avec la bioinformatique visant elle à analyser les résultats.
PCT/US2000/011460 1999-04-28 2000-04-28 Identification d'elements de controle d'adn reagissant a des stimuli specifiques Ceased WO2000065039A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU46754/00A AU4675400A (en) 1999-04-28 2000-04-28 Identification of dna control elements responsive to specific stimuli

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US13143399P 1999-04-28 1999-04-28
US60/131,433 1999-04-28
US14466999P 1999-07-19 1999-07-19
US60/144,669 1999-07-19

Publications (2)

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WO2000065039A2 WO2000065039A2 (fr) 2000-11-02
WO2000065039A3 true WO2000065039A3 (fr) 2001-02-08

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AU (1) AU4675400A (fr)
WO (1) WO2000065039A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7005255B2 (en) 2000-04-14 2006-02-28 Metabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
CN109234284B (zh) * 2018-09-14 2021-04-09 昆明理工大学 一种三七类甜蛋白基因PnTLP5及应用

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001027321A2 (fr) * 1999-10-13 2001-04-19 Plant Research International B.V. Procede pour detecter les differences dans l'expression genique de plantes de type sauvage et de plantes genetiquement modifiees
WO2002072789A2 (fr) * 2001-03-12 2002-09-19 Irm, Llc. Essais biologiques cellulaires grande vitesse fondes sur la genomique, elaboration de ces essais, et collections de rapporteurs cellulaires
WO2002090516A2 (fr) * 2001-05-07 2002-11-14 Amersham Biosciences Corp Conception de genes artificiels a utiliser en tant que temoins dans des systemes d'analyse d'expression genique
US8849577B2 (en) 2006-09-15 2014-09-30 Metabolon, Inc. Methods of identifying biochemical pathways

Citations (2)

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Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5851766A (en) * 1995-05-31 1998-12-22 Novartis Finance Corporation Process for isolating chemically regulatable DNA sequences
DE19860313A1 (de) * 1998-12-24 2000-06-29 Friedrich Felsenstein Verfahren zur Erkennung und Charakterisierung von Wirkstoffen gegen Pflanzen-Pathogene

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7005255B2 (en) 2000-04-14 2006-02-28 Metabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7550258B2 (en) 2000-04-14 2009-06-23 Metabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7550260B2 (en) 2000-04-14 2009-06-23 Metabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7553616B2 (en) 2000-04-14 2009-06-30 Metabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7635556B2 (en) 2000-04-14 2009-12-22 Cornell Research Foundation, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7947453B2 (en) 2000-04-14 2011-05-24 Metabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
CN109234284B (zh) * 2018-09-14 2021-04-09 昆明理工大学 一种三七类甜蛋白基因PnTLP5及应用

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WO2000065039A2 (fr) 2000-11-02

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