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WO2000063429A3 - Ein automatisierbarer schnelltest zum nachweis von krebserkrankungen auf der basis der telomerase(htc) mrna mit spezifischen primern und sonden - Google Patents

Ein automatisierbarer schnelltest zum nachweis von krebserkrankungen auf der basis der telomerase(htc) mrna mit spezifischen primern und sonden Download PDF

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Publication number
WO2000063429A3
WO2000063429A3 PCT/EP2000/002980 EP0002980W WO0063429A3 WO 2000063429 A3 WO2000063429 A3 WO 2000063429A3 EP 0002980 W EP0002980 W EP 0002980W WO 0063429 A3 WO0063429 A3 WO 0063429A3
Authority
WO
WIPO (PCT)
Prior art keywords
automatable
htc
telomerase
mrna
basis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/002980
Other languages
English (en)
French (fr)
Other versions
WO2000063429A2 (de
Inventor
Wolfgang Springer
Gustav Hagen
Maresa Wick
Dmitry Zubov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Original Assignee
Bayer AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG filed Critical Bayer AG
Priority to JP2000612506A priority Critical patent/JP2003519466A/ja
Priority to CA002370305A priority patent/CA2370305A1/en
Priority to AU41153/00A priority patent/AU774182B2/en
Priority to US09/959,014 priority patent/US6808883B1/en
Priority to EP00920657A priority patent/EP1187936A2/de
Publication of WO2000063429A2 publication Critical patent/WO2000063429A2/de
Anticipated expiration legal-status Critical
Publication of WO2000063429A3 publication Critical patent/WO2000063429A3/de
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Hospice & Palliative Care (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft einen automatisierbaren Schnelltest zum Nachweis von Krebserkrankungen auf der Basis der Telomerase(hTC) mRNA, geeignete Starternukleotide und Oligonukleotidsonden für diesen Test sowie ein entsprechendes Nachweisverfahren und ein Testkit.
PCT/EP2000/002980 1999-04-15 2000-04-04 Ein automatisierbarer schnelltest zum nachweis von krebserkrankungen auf der basis der telomerase(htc) mrna mit spezifischen primern und sonden Ceased WO2000063429A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2000612506A JP2003519466A (ja) 1999-04-15 2000-04-04 テロメラーゼ（ｈＴＣ）ｍＲＮＡおよび特異的プライマーおよびプローブに基づいたガンを検出するための自動化可能な迅速な試験
CA002370305A CA2370305A1 (en) 1999-04-15 2000-04-04 Automatable rapid test for detecting cancers, on the basis of telomerase(htc) mrna and specific primers and probes
AU41153/00A AU774182B2 (en) 1999-04-15 2000-04-04 Automatable rapid test for detecting cancers, on the basis of telomerase(hTC) mRNA and specific primers and probes
US09/959,014 US6808883B1 (en) 1999-04-15 2000-04-04 Automatable rapid test for detection of cancer, based on telomerase (hTC) mRNA with specific primers and probes
EP00920657A EP1187936A2 (de) 1999-04-15 2000-04-04 Ein automatisierbarer schnelltest zum nachweis von krebserkrankungen auf der basis der telomerase(htc) mrna mit spezifischen primern und sonden

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19916929A DE19916929A1 (de) 1999-04-15 1999-04-15 Ein automatisierbarer Schnelltest zum Nachweis von Krebserkrankungen auf der Basis von Telomerase(hTC) mRNA mit spezifischen Primern und Sonden
DE19916929.2 1999-04-15

Publications (2)

Publication Number Publication Date
WO2000063429A2 WO2000063429A2 (de) 2000-10-26
WO2000063429A3 true WO2000063429A3 (de) 2002-01-03

Family

ID=7904587

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/002980 Ceased WO2000063429A2 (de) 1999-04-15 2000-04-04 Ein automatisierbarer schnelltest zum nachweis von krebserkrankungen auf der basis der telomerase(htc) mrna mit spezifischen primern und sonden

Country Status (7)

Country Link
US (1) US6808883B1 (de)
EP (1) EP1187936A2 (de)
JP (1) JP2003519466A (de)
AU (1) AU774182B2 (de)
CA (1) CA2370305A1 (de)
DE (1) DE19916929A1 (de)
WO (1) WO2000063429A2 (de)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0420260A2 (de) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Durch Polymerase-Kettenreaktion Biotin-markierte DNS und ihr Nachweis
WO1991014788A1 (en) * 1990-03-23 1991-10-03 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
GB2317891A (en) * 1996-10-01 1998-04-08 Geron Corp hTRT, the reverse transcriptase subunit of human telomerase
WO1998059040A2 (de) * 1997-06-20 1998-12-30 Bayer Aktiengesellschaft Humane katalytische telomerase-untereinheit und deren diagnostische und therapeutische verwendung
WO1999001560A1 (en) * 1997-07-01 1999-01-14 Cambia Biosystems Llc Vertebrate telomerase genes and proteins and uses thereof
WO1999035261A1 (en) * 1998-01-08 1999-07-15 Chugai Seiyaku Kabushiki Kaisha Novel gene having reverse transcriptase motif
WO1999050279A1 (en) * 1998-03-31 1999-10-07 Geron Corporation Antisense compositions for detecting and inhibiting telomerase reverse transcriptase
US5994076A (en) * 1997-05-21 1999-11-30 Clontech Laboratories, Inc. Methods of assaying differential expression
WO2000027858A1 (en) * 1998-11-09 2000-05-18 Institute Of Radiation Medicine, Academy Of Military Medecine Science Antisense oligonucleotides which inhibit telomerase's activity and their uses

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4957858A (en) 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
NO904633L (no) 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0420260A2 (de) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Durch Polymerase-Kettenreaktion Biotin-markierte DNS und ihr Nachweis
WO1991014788A1 (en) * 1990-03-23 1991-10-03 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
GB2317891A (en) * 1996-10-01 1998-04-08 Geron Corp hTRT, the reverse transcriptase subunit of human telomerase
US5994076A (en) * 1997-05-21 1999-11-30 Clontech Laboratories, Inc. Methods of assaying differential expression
WO1998059040A2 (de) * 1997-06-20 1998-12-30 Bayer Aktiengesellschaft Humane katalytische telomerase-untereinheit und deren diagnostische und therapeutische verwendung
WO1999001560A1 (en) * 1997-07-01 1999-01-14 Cambia Biosystems Llc Vertebrate telomerase genes and proteins and uses thereof
WO1999035261A1 (en) * 1998-01-08 1999-07-15 Chugai Seiyaku Kabushiki Kaisha Novel gene having reverse transcriptase motif
WO1999050279A1 (en) * 1998-03-31 1999-10-07 Geron Corporation Antisense compositions for detecting and inhibiting telomerase reverse transcriptase
WO2000027858A1 (en) * 1998-11-09 2000-05-18 Institute Of Radiation Medicine, Academy Of Military Medecine Science Antisense oligonucleotides which inhibit telomerase's activity and their uses

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CONG YU-SHENG ET AL: "The human telomerase catalytic subunit hTERT: Organization of the gene and characterization of the promoter.", HUMAN MOLECULAR GENETICS, vol. 8, no. 1, January 1999 (1999-01-01), pages 137 - 142, XP002176111, ISSN: 0964-6906 *
WICK M ET AL: "Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT).", GENE (AMSTERDAM), vol. 232, no. 1, 17 May 1999 (1999-05-17), pages 97 - 106, XP002176112, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
CA2370305A1 (en) 2000-10-26
AU774182B2 (en) 2004-06-17
US6808883B1 (en) 2004-10-26
WO2000063429A2 (de) 2000-10-26
EP1187936A2 (de) 2002-03-20
JP2003519466A (ja) 2003-06-24
AU4115300A (en) 2000-11-02
DE19916929A1 (de) 2000-10-19

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