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WO2000060118A3 - Electrophoretic analysis of target molecules using adapter molecules - Google Patents

Electrophoretic analysis of target molecules using adapter molecules Download PDF

Info

Publication number
WO2000060118A3
WO2000060118A3 PCT/US2000/008529 US0008529W WO0060118A3 WO 2000060118 A3 WO2000060118 A3 WO 2000060118A3 US 0008529 W US0008529 W US 0008529W WO 0060118 A3 WO0060118 A3 WO 0060118A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
adapter
electrophoretic analysis
target
target molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/008529
Other languages
French (fr)
Other versions
WO2000060118A2 (en
Inventor
Lawrence Weir
T Christian Boles
Ezra S Abrams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mosaic Technologies Inc
Original Assignee
Mosaic Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies Inc filed Critical Mosaic Technologies Inc
Priority to CA002371727A priority Critical patent/CA2371727A1/en
Priority to JP2000609607A priority patent/JP2003508015A/en
Priority to EP00919931A priority patent/EP1235930A2/en
Priority to AU40539/00A priority patent/AU4053900A/en
Publication of WO2000060118A2 publication Critical patent/WO2000060118A2/en
Priority to US09/968,084 priority patent/US20020197614A1/en
Anticipated expiration legal-status Critical
Publication of WO2000060118A3 publication Critical patent/WO2000060118A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A universal gel and methods of use in detecting the presence, or absence, of one or more target molecules in a test sample comprising the formation of a universal gel hybridization complex, wherein an adapter molecule is hybridized to a target molecule and universal capture probe is disclosed.
PCT/US2000/008529 1997-05-16 2000-03-31 Electrophoretic analysis of target molecules using adapter molecules Ceased WO2000060118A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002371727A CA2371727A1 (en) 1999-04-02 2000-03-31 Electrophoretic analysis of target molecules using adapter molecules
JP2000609607A JP2003508015A (en) 1999-04-02 2000-03-31 Electrophoretic analysis of target molecules using adapter molecules
EP00919931A EP1235930A2 (en) 1999-04-02 2000-03-31 Electrophoretic analysis of target molecules using adapter molecules
AU40539/00A AU4053900A (en) 1999-04-02 2000-03-31 Electrophoretic analysis of target molecules using adapter molecules
US09/968,084 US20020197614A1 (en) 1997-05-16 2001-10-01 Electrophoretic analysis of target molecules using adapter molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28538099A 1999-04-02 1999-04-02
US09/285,380 1999-04-02

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US28538099A Continuation 1997-05-16 1999-04-02

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/968,084 Continuation US20020197614A1 (en) 1997-05-16 2001-10-01 Electrophoretic analysis of target molecules using adapter molecules

Publications (2)

Publication Number Publication Date
WO2000060118A2 WO2000060118A2 (en) 2000-10-12
WO2000060118A3 true WO2000060118A3 (en) 2002-07-11

Family

ID=23093992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/008529 Ceased WO2000060118A2 (en) 1997-05-16 2000-03-31 Electrophoretic analysis of target molecules using adapter molecules

Country Status (5)

Country Link
EP (1) EP1235930A2 (en)
JP (1) JP2003508015A (en)
AU (1) AU4053900A (en)
CA (1) CA2371727A1 (en)
WO (1) WO2000060118A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE335845T1 (en) 1997-05-16 2006-09-15 Exact Sciences Corp ELECTROPHORETIC ANALYSIS OF MOLECULES USING IMMOBILIZED PROBE
AU4189800A (en) * 1999-04-02 2000-10-23 Exact Sciences Corporation Methods of detecting microorganism using immobilized probes
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
ATE551433T1 (en) * 2005-05-06 2012-04-15 Gen Probe Inc METHOD AND PRODUCTS FOR DETECTING NUCLEIC ACID TARGETS
US8492084B2 (en) 2006-01-24 2013-07-23 Koji Sode Method and apparatus for assaying test substance in sample
EP2115455B1 (en) * 2007-02-07 2012-02-22 Lyzer Diagnostics, Inc. A rapid homogeneous immunoassay using electrophoresis
US9976984B2 (en) * 2013-05-10 2018-05-22 The Regents Of The University Of California Free-standing microfluidic gel electrophoresis devices and methods

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0347097A (en) * 1989-07-13 1991-02-28 Hitachi Ltd Hybridization method, gene mutation detection using same and apparatus therefor
WO1994006937A1 (en) * 1992-09-15 1994-03-31 Boehringer Mannheim Corporation Improved strand displacement assay and complex useful therefor
WO1997031256A2 (en) * 1996-02-09 1997-08-28 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1998039351A1 (en) * 1997-03-05 1998-09-11 Mosaic Technologies, Inc. Nucleic acid-containing polymerizable complex
WO1998051823A1 (en) * 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
WO1999066079A1 (en) * 1998-06-19 1999-12-23 Mosaic Technologies Detection of non-viral organisms with srp rna

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0347097A (en) * 1989-07-13 1991-02-28 Hitachi Ltd Hybridization method, gene mutation detection using same and apparatus therefor
WO1994006937A1 (en) * 1992-09-15 1994-03-31 Boehringer Mannheim Corporation Improved strand displacement assay and complex useful therefor
WO1997031256A2 (en) * 1996-02-09 1997-08-28 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1998039351A1 (en) * 1997-03-05 1998-09-11 Mosaic Technologies, Inc. Nucleic acid-containing polymerizable complex
WO1998051823A1 (en) * 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
WO1999066079A1 (en) * 1998-06-19 1999-12-23 Mosaic Technologies Detection of non-viral organisms with srp rna

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KENNEY MARY ET AL: "Mutation typing using electrophoresis and gel-immobilized acrydite probes.", BIOTECHNIQUES, vol. 25, no. 3, 1998, pages 516 - 521, XP002159876, ISSN: 0736-6205 *
PATENT ABSTRACTS OF JAPAN vol. 015, no. 189 (C - 0831) 15 May 1991 (1991-05-15) *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm

Also Published As

Publication number Publication date
JP2003508015A (en) 2003-03-04
EP1235930A2 (en) 2002-09-04
WO2000060118A2 (en) 2000-10-12
CA2371727A1 (en) 2000-10-12
AU4053900A (en) 2000-10-23

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