WO2000054750A1 - Method for correcting disturbance in collagen synthesis and biologically active substance for realising the same - Google Patents

Abstract

The present invention relates to a method for correcting disturbance in collagen synthesis that involves hydroxylating proline and/or lysine. The proline and/or lysine are previously hydroxylated outside the patient's organism, are included into liposomes and are further introduced parenterally into the patient's organism. This invention also relates to a biologically active substance for realising the method for correcting collagen synthesis, wherein said substance includes lanolin or glycerin, water as well as a liposome emulsion containing an active ingredient. The active ingredient contained in the liposomes consists of hydroxylated proline and/or lysine, and the components are selected in the following amounts: from 50.0 to 70.0 wt % of lanolin or glycerin; from 10.0 to 15.0 wt % of liposomes in the form of a 10-20 % emulsion containing a 10-30 % aqueous solution of hydroxylated proline and/or lysine; the balance consisting of water. The liposomes may further contain an amount of 0.5 to 1.5 wt % of an anti-oxidising agent that may consist of ionol.

Description

 A method for disturbing the synthesis of collagen and

biological active material for its implementation

Area of technology

The invention is related to medicine and may be

used in the treatment of diseases caused by

by violation of the process of synthesis of collagen in human society, in

PARTICULARITIES, PRODUCTS, DEVICES, VARIOUS PARTICIPANTS,

as well as in the method for the treatment of the withering process of the skin.

PREVIOUS LEVEL OF TECHNOLOGY

In the process of the vital activity of humanism under the influence

unfavorable factors are involved in the decay process

Ad hoc collagen, in addition there is a place and violation

synthesis of wolves, in particular, owing to the lack of sufficient

hydration of essential amino acids 2 lysines. With this synthesis, halogen-free wolves have

The immediate structure, which leads to the development of a number of diseases:

aromatics, dermatoses, various forms of care. Κροме τοгο,

due to the influence of external and external factors (temperature,

ultraviolet radiation, low humidity,

microparticles, etc.), the skin withers, and also

the content of defective collagen is increasing.

The methods of synthesis of the collagen are known.

by introducing a basic controller, the main component of the card

is glycosaminoglycan, a high molecular weight polysaccharide

(Κυ, C 1, 2020907).

This is a convenient way of working.

It activates the synthesis process of collagen only in

quantitatively disregarding and does not indicate inconvenient

effects on quality synthesizable collagen waves. This

It is explained that glucose glitch is due to the high

molecular weight does not penetrate through cell membranes

Fibers, in turn, synthesize collagen. Κροме τοгο,

This method does not prevent decay processes. unhealthy collagen.

The method of disturbing the synthesis of collagen is known.

by the introduction of ρpostaglandin Ε2, which is a stimulant

a gene - an active process of the synthesis of collagen (ϋϊ Β Β ύ ύ ϊ ϊ; aϊ.

Δгοδ1; а§1аηёϊη Ε2 ϊшшϊаϊез ϊηсοφοгаϊюη ο! ^" ρгοПηе ϊη Ιο сο11а £ еηе

ϊ§Ιϊ ϊ ϊ δ δ δ δ δ δ η δ δ δ δ. // "Ιοигηаϊ οϊ

Μοϊesiϊag seϊϊiϊag eηάοsπηο1ο§u ", 1996, Uοϊ.123, ϋΥe 1, Ρ.27 -35,

Μοηϊgeaϊ, Caηaάa). In realizing this method

ρprostostaglandin Ε2 has a stimulating effect on the gene,

which is an active process in the synthesis of collagen.

Accessible data is a public service,

that prostaglandin Ε2 only “starts” one of the phases of the synthesis

It is collagen, but it does not support its long life,

so how quickly, within a second, is destroyed

features. For the sake of realizing the need for a permanent

intense intake of prostaglandin Ε2, which is quite

having a good stay. Τρome me, in connection with an emergency

the absence of prostaglandin Ε2 reveals the impact only in

very small area at the injection site, which is necessary do a lot.

The method of disturbing the synthesis of collagen in

human society by influencing the process

hydroxylation of amino acid and / or lysine (Ζig

аиϊ άазΑΙϊο1ο§1е ёег СгϊЬга οгЫϊаΗа:

aiϊ άаз

ШιшηοδаигергоШ ϊт кηοсЬеееηкο11агеη. “Ζ.ΜοφЬο1ο§ϊе andηά

aηϊгοροϊοёϊе », 1995, Βаησ! 81, Νδ 1, 3.125 - 137, ΜиηсЬеη,

ΟeiΙsYaηά). In the implementation of the method in human society

ions of iron and steel ( ³⁺ ) and acid,

enteric or parenteral. Ρe ⁺ sποsοbsτvuyuτ οτscheπleniyu

HYDROGEN ION (Ρ-Г) οτ molecules of acid, which

necessary for hydration of προline and lysine; this

It is required to ensure the synthesis of collagen and to prevent

Recognition of riches in halogenous volokon.

This is a convenient way of working.

It provides, in the main, only an initial stage

The process of hydration of amino acids and the synthesis of collagen.

For the effective completion of this process, the process is necessary

Sufficiently high activity of hydroxylases of amino acids and 5 Prevention of the loss of immunity

activated activates. Self introduction

iron and ascorbic acid in no way affect the activity

indicated above complex components, which depends on

many factors related to the state of the organism,

Accordingly, the decay of immature fractions is not affected,

that complicates the process of “ripening” of the collagenous wave.

Therefore, this method is not sufficiently effective.

Well-known biologically active substances - cosmetic

Medium for the regeneration of withering skin (ΙШ, С1, 2036637),

containing lanolin, vegetable oil, glycerin, ethyl

sirupt, lecithin, methyl ester of steamed hydroxybenzoic acid,

steam room, toilet, water, cosmetic, cosmetic stearin,

distant monoglycerides; symptoms of the function Сι ₇ С _{ϊ 8}

Dispersed, or high-molecular-weight synthetics

Fraction Сι С _ϊ8 , or highly synthetic synthetics

primary higher fatty fraction C _{ϊ b} C ₂ ο, calcium hydroxide,

ethereal hydroxybenzoic acid; in quality

The active beginnings use a 10% emulsion, 6 containing 0.1% of alpha-fatty acid, and in the quality of industrial

Oils - Olive Refined Oils, or Salad

Adequate or subsidiary

high olein and the next

Comparison of components, mass%:

Olive Refined Oil, or Salad Olive

Decomposed, or subsidiary, high-olein

deodorized - 6.0;

cosmetic stearin metro Α.Β. D.U. - 4.6;

distant monoglycerides - 3.4;

features of the function Сι ₇ Сι ₈ identified

(synthesized primary) C _{] 6} C ₂ ο

primary higher fatty functions Сι ₆ С ₂ ο - 1.00;

oxyethylated lanolin - 60.00;

potassium hydroxide - 0.30;

distilled glycerol - 3.00;

ethyl alcohol - reducts - 5.00;

lecithin, 50% solution - 2.00;

methyl ester of para-sibenzoic acid - 0.30; 7 acid ester of parabenzoic acid - 0.15

πаρаφορм - 0.05

room - 1.00

liposomes, 10% suspension with 0.1%

alpha-τοκοφеροла -10.00;

WATER - OTHER.

The cost of this material is its small

EFFICIENCY, BECAUSE IT DOES NOT SHOW ANYTHING - Either

impact on the process of synthesis of collagen, violation of the process

0 is a fact that separates the process of withering of the skin, and

Also, the pathogenesis of dermatoses, arteries and the patient. Immediately

It indicates that the operation is mainly on a regular basis,

epidemiological layer of the skin.

Also known is biologically active material for

5 regeneration of τκanes of human organism (Κυ, С1, 2045261),

Containing Olive Refined Oil, or Salad Oil

Adequate or subsidiary

Highly oleinous, syrups of function Сι ₇ С _ϊ

Residual Synthetic Primary, or Syringes 8 high molecular weight fractions Сι ₆ Сι ₈ , or

synthetic primary higher fattype

Сι ₆ Сϊ8, oxyethylated lanolin, shower, water,

cosmetic stearin metro Α.Β. D.U., monoglycerides

distilled, potassium hydroxide, glycerin

distilled, stylish ethical product of the highest grade,

lecithin 50% aqueous solution, effective πpropyl

HYDROXYBENIC ACID, ETHYR METAL HYDROXYBENZIDE

Acids, emulsion of liposomes containing 2.5 - 5.0% of the total

extract from the culture of a ginseng cellulose, steamed parsley

the following ratio of components, mass%:

Olive Refined Oil, or Salad Oil

Adequate, or oil, subsidiary

high oleinous - 6.00;

The cosmetic stearin of the brand Α.Β. D.U. - 4.60;

distant monoglycerides - 3.40;

features of the function Сι ₇ Сϊ ₈ driven

synthetic primary -1.10; 9 high molecular weight syntheses

φρaκtsii C _ϊ bSgο ,, or sinτeτichesκie

primary higher fatty and "lφοl

the functions Сι ₆ С ₂ ο - 1.00;

lanoline oxyethylated - 60.00;

hot potassium hydroxide - 0.30;

glycerol distilled - 3.00;

top-grade ethical clerk - 5.00;

lecithin, 50% solution - 2.0 0;

ethereal methyl steamed acid - 0.30;

ethereal hydroxybenzoic acid - 0.15;

πаρаφορм - 0.05;

shower - 1.00;

Suspension of lime, containing 2.5-5.0% of the annual

extract from the ginseng cell culture - 10.00;

WATER - OTHER.

Only softening and moisturizing are provided.

κοzh; to deep processes that are actually occurring

In the final case, both basic and conditional, in the main, 10 synthesis of collagen, it doesn’t indicate any impact, and,

There is no valid treatment for disease,

Due to disturbance of processes of collagen synthesis.

DISCLOSURE OF INVENTION

BACKGROUND OF THE INVENTION

tasks of increasing the efficiency of the method of synthesis synthesis

collagen in the human body, independent of the activity

Hydroxylases and erythrocytes of kollagenovyh tkane

The decay of an ill-defined collagen, as well as the building

0 Efficient Biologically Active Substances for Realization

The method of compilation of the collagen synthesis in the human body by

Avoiding the impact of this substance on biochemical

process of synthesis of collagen.

According to the invention, this task is solved due to the fact that

in the process of disturbance of the synthesis of collagen in the organism

a person by hydration of ipline and / or lysine,

προline and / or lysine is predominantly hydrosylated outside 11 of the human body, concludes in the licenses and then introduces

Parenteral in humanism.

Л biological active material for the implementation of the method

a section of the synthesis of collagen, including lanolin or

glycerin, water and emulsion of liposomes containing active

beginnings, the customers on a rolling basis start up

hydrolyzed προlin and / or lysine πρ and the following

incidence of components, mass%:

lanolin or glycerol - 50.0 -70.0;

Limes 10-20% emulsion with 10-30% water

automatic hydraulics and / or

lysine - 10.0 - 15.0;

water - other;

people can keep antioxidants in quantities of 0.5 - 1.5

mass%; as an antioxidant, an ionol may be used.

The applicant has not established any - or any sources

information that would contain information on technical

Decisions identical to those declared. This pleases, on opinion

Applicant to conclude that the invention is in accordance with the invention 12 “novelty” (Ν).

Realization of distinctive characteristics of the living

INVENTIONS (IN ACCORDANCE WITH THE SIGNS ARRANGED IN

of the limited part of the formula of the invention)

Recognition of a fundamentally important new property of the object,

except for influence on the process

amine acid hydroxide and fatty acid decomposition

Facts that affect the state of humanism. By what

Efficiency of the process of hydration is ensured.

amino acid in allogenic tissues from its beginning and to the end, and,

Accordingly, an increase in the content of hydraulic power

Amino Acid in Hellagenic Tactics and Decrease Decay

unhealthy collagen. Thanks to this, significantly increased

the degree of synthesis of collagen in the organism, which is very

important in the treatment of related diseases.

There is a direct impact on the synthesis process

complete collagen, which contributes to the commencement of full-fledged waves.

This makes it possible to drastically slow down the withering of the skin, and also

It has a positive effect on the treatment of a number of diseases, 13 caused by disturbance of the synthesis of collagen in humanism.

Applicant has not identified any information about influence

Distinctive features of the invention

technical result. Indicated OWNERS

make a conclusion on the basis of the declared technical solution

The term “inventive step” (18).

BEST MODE FOR CARRYING OUT THE INVENTION

The claimed method is implemented by the following method.

Κ лин κ κ κ κ κ лин лин лин κ κ κ

Primarily by means of hydrolysis

connective tissue (uterine, connective, tendon, abdominal

vessels) of pigs and domestic cattle. Аче Quality of the enzyme

used τρипсин. The explanation is in

special capacities - features available on

meat processing plants, where safe food is provided

Industrial Cycle Other options are also different.

Amino Acid of an Immobilized Synthesis. Β 14 parts, enhanced synthesizing

Recombinant plasmid containing Ρзϊϊ is a vector component

plasmids ΜΤΜΤ and Ρδύ - ρ ρ аг мен мен мен мен мен мен мен мен ΗΚ ΗΚ ΗΚ ΗΚ ΗΚ

104 Law of the USSR δυ 1604, established at the Institute of Molecular

genetics ΡΑΗ.

The urine and / or lysine is then hydrolyzed ϊη νϊϊгο

5% of the processing industry in the Republic of Kazakhstan in the manufacture of

Acidic Acid and iron ions. Process

They are carried out for 60 minutes at a temperature of 37 ° C. Then

Hydroxylated hydrochloride ester (in this example

A combination of 50% and 50% is used.

lysine) is included in the usual conventionally known methods. Β

in particular, in the case of lipids in diethyl dispersion

20% WATER CONTAINER OF PRINLINE AND LYSINE BY PROCESSING

ultrasound for 3-5 minutes. Then remove the diethyl ether

the method of the Cold Rider. The resulting suspension is diluted

Spatial and saline buffered and centered.

Received liposomes containing προline and lysine, several

I wash away the water - saline. 15 In addition to amino acids, there may be

the antioxidant is excluded, in particular, the contributor

protect amino acids from the impact of free radicals.

Lipsom. containing προline and / or lysine, for convenience

applications, place in a viscous medium, for example lanolin or

glycerin, and in this case they enter into humanity of the neighbor

applications. For injection of liposomes into an organism

Disposed in a liquid medium, such as an industrial fluid

ΝАСΙ, on the other hand, 10% suspension with

hydroxylated amino acids.

Particular examples of synthesis disturbance

collagen in human society and some pathology

By means of the implementation of the claimed method, please see below.

EXAMPLE 1. BN Α., 22 years old, I was diagnosed with a diagnosis:

arthritis - arthritis of the local lobe joint (elbow of tennism).

Belen 2 years. Β τρχ months προ τρ and κуρс

applications for the area of the local articulation for ten cases

every day with every meal for twenty days. By

At the end of treatment, disappearance of the disease was observed 16 joint and arthralgia, increased mobility of the joint. Relapse

for 6 months was not observed.

Example 2. Patient Μ. 43 years old, diagnosed - on the 2nd

degree, sick for 11 years. Persons with prisoners in

they were introduced by hydrated praplinin and lysine into

takan of the consumer of the applications. Five hoses

πο 12 προ procedure with weekly transitions. Лечения result of treatment

gum status improved significantly: decreased

extremities and volatility. Black and white

systemic administration of anti-relapse therapy.

Alternatively, injection of liposomes into

ορorganism. For this you can use 10% suspension in bulk

from the industrial sector.

The essence of the claimed substance is explained by its examples.

concomitant use. The examples are shown in table χ:

- in table 1 the values of the components are presented

biological active substances used in groups I - III;

- in table 2 - the results achieved in the results

Results. 17

Table 1

Table 2

18 Были προведены сρавниτельные исπыτания на живοτныχ с

18 Comparative tests were carried out on live animals.

the purpose of studying the efficacy of the drug (the process component)

synthesis of collagen at the content of a healthy native collagen in

each day), as well as studying off-road

Annoying and Allergy. For pricing

The results of the synthesis of collagen synthesized method

π polarity; in terms of speed, it is immediately done

the living room at the place of exposure to the drug, the cut sample

it is laid on a small glass, it is called a simple glass

and is moving on to a complex consisting of

The popular world of light in this

πρimerе - "Polam -L-213"

PETROBURG, ANALOGUE-DIGITAL CONNECTOR, PARTICULAR

Ρаскагά" и πеρсοнальнοгο117-Μ "production

Ρaskagά "and π ρ ρ ρ ρ ρ льн и и

the company type ΙΒΜ "ύеηύшη". Contribution of a lively native

kollagena expressed in conditional units,

degree of content in the tide. Τ ο τ τ τ,, explored

It is also possible to keep the product in good condition.

the famous method of ΚaΚ Κ.Υ., Μсθаνask Τ.Η.// Ρgos. δοс. Εχρ. 19 Βюϊ. Άеά ..- 1959.- УοΙ.ΙΟΙ, .- Ρ.153-161., Founded on

hydrolysis of an indirect collagen in the 0.1-normal condition of the ΝaΟΗ

within 24 hours πρ and temperature 25 ° С. Was also given προ

Estimation of the skin and liver structure taken from

TESTED ANIMALS.

Researches have been conducted on male pigs - males

weighing 180 - 300 g, standard condition

vivarium. Five experimental groups were formed

live: 3 groups with an increase of 1.5 years, which was applied

preparation in the form of an application (Ι-ΙΙΙ) (old “experienced” animals),

old intact livestock 1.5 years (IV), young

intact livestock 6 months. (V). Contents

components of biological active material used in

GROUPS I - III, presented in Table 1. The preparations were applied

daily for 30 days on a pre-paid basis

On the other hand, the side of the body is lively.

The results of the experiments are summarized in Table 2.

From table 2 it is visible that the application is biological

active substance containing prisoners 20 hydrated naphtha and lysine, substantially contributes to

Raise the content of a mature, natu- ral collagen in other countries

lively. The development of deficient immunity is being promoted.

The collagen form, which is the indispensable basis for

The subsequent development of a fully valuable mature collagen.

There is a slight increase in the efficiency of the impact

biologically active material with an increase in content in

it is hydrated with amino acids - προline and / or lysine.

In test animals, no cases have been established

Annoying or allergic to each other.

Intended use

For the implementation of the invention, conventional

technical substances, known materials, and other products

Processes, in connection with which it is possible to make a withdrawal of compliance

The invention is a combination of the “intended use” (ΙΑ).

Claims

21Formula of the invention 1. The method of breaching the disturbance of the synthesis of collagen by hydrolysis of προline and / or lysine, characterized that is, pi-lin and / or lysine is hydroxy-reinforced outside the body of a person, enter into the licenses and then introduce Parenteral in humanism. 2. Biologically active substances for the implementation of the method a section of the synthesis of collagen, including lanolin or glycerol, an emulsion of liposomes containing the active principle, and the water, which differs in terms of quality beginnings of the hydropoxylated feedline and / or lysine of the following and the following ratios of components, mass%: Lanolin or glycerol 50.0 - 70.0 Lips 10 - 20%> emulsion with 10 - 30% water-based hydraulics προline and / or lysine 10.0 - 15.0 The rest is food. 22 3. Biologically active material π π.2, W ith r a t e n c e, that is why antioxidant in the amount of 0.5 - 1.5 mass%. 4. Biologically active substances πpo3, Including with antioxidant quality used by an ionol.