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WO2000050880A3 - Improvements in or relating to microfluidic sample preparation and mass spectrometry - Google Patents

Improvements in or relating to microfluidic sample preparation and mass spectrometry Download PDF

Info

Publication number
WO2000050880A3
WO2000050880A3 PCT/GB2000/000625 GB0000625W WO0050880A3 WO 2000050880 A3 WO2000050880 A3 WO 2000050880A3 GB 0000625 W GB0000625 W GB 0000625W WO 0050880 A3 WO0050880 A3 WO 0050880A3
Authority
WO
WIPO (PCT)
Prior art keywords
mass spectrometer
sample
macromolecular assemblies
gas
diverted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/000625
Other languages
French (fr)
Other versions
WO2000050880A2 (en
Inventor
Mark Anthony Tito
Carol Vivien Robinson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford University Innovation Ltd
Original Assignee
Oxford University Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford University Innovation Ltd filed Critical Oxford University Innovation Ltd
Publication of WO2000050880A2 publication Critical patent/WO2000050880A2/en
Publication of WO2000050880A3 publication Critical patent/WO2000050880A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • H01J49/167—Capillaries and nozzles specially adapted therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/60—Pump mixers, i.e. mixing within a pump
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/75—Discharge mechanisms
    • B01F35/754—Discharge mechanisms characterised by the means for discharging the components from the mixer
    • B01F35/75435—Discharge mechanisms characterised by the means for discharging the components from the mixer using means for discharging the mixture in a pulsating or intermittent manner
    • B01F35/754351—Discharge mechanisms characterised by the means for discharging the components from the mixer using means for discharging the mixture in a pulsating or intermittent manner using electrical pulses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Plasma & Fusion (AREA)
  • Analytical Chemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

An electrospray ionisation probe (100) which comprises a piezoelectric micropump for receiving two liquid components, mixing them to form a sample and ejecting them into a capillary needle which, in turn, emits the mixture as an ionised spray to a mass spectrometer for analysis. The drive voltage for the piezoelectric micropump can be varied to vary the mixing parameters. Temperature regulation of the sample is provided by a thermoelectric device in the probe. Bath gas can be passed through channels of an aluminum block attached to the thermoelectric device to provide temperature controlled gas which is supplied to an apron surrounding the sampling cone of the mass spectrometer. This provides temperature regulation of the sample. Another aspect of the invention provides a method of collecting macromolecular assemblies by using a time-of-flight mass spectrometer in which the ion beam containing packet, containing the macromolecular assemblies is diverted by an orthogonal accelerator (910). The diverted ions are collected on a collector plate (920) which can be an EM grid or X-ray plate, to be used for further analysis. A double isolation valve arrangement is used so that the collection plate (920) can be removed without venting the interior of the mass spectrometer. Macromolecules can be detected and collected intact by admitting gas into the mass analyser to effect collisional cooling namely reducing the internal energy of the ionized macromolecular assemblies.
PCT/GB2000/000625 1999-02-22 2000-02-22 Improvements in or relating to microfluidic sample preparation and mass spectrometry Ceased WO2000050880A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9904038.8A GB9904038D0 (en) 1999-02-22 1999-02-22 Improvements in or related to microfluidic sample preparation and mass spectrometry
GB9904038.8 1999-02-22

Publications (2)

Publication Number Publication Date
WO2000050880A2 WO2000050880A2 (en) 2000-08-31
WO2000050880A3 true WO2000050880A3 (en) 2000-12-07

Family

ID=10848268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/000625 Ceased WO2000050880A2 (en) 1999-02-22 2000-02-22 Improvements in or relating to microfluidic sample preparation and mass spectrometry

Country Status (2)

Country Link
GB (1) GB9904038D0 (en)
WO (1) WO2000050880A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0020487D0 (en) 2000-08-18 2000-10-11 Aea Technology Plc Moving liquid into and along micro-fluidic channels
DE10116979A1 (en) * 2001-04-05 2002-10-17 Wollnik Hermann Ion storage device for flight time mass spectrometer uses DC potential trough in which ions oscillate for cooling by impact with residual gas atoms
WO2017154153A1 (en) * 2016-03-09 2017-09-14 株式会社島津製作所 Mass spectrometer and biological sample analysis method using said mass spectrometer
GB201815746D0 (en) * 2018-09-27 2018-11-14 Imperial Innovations Ltd High-pressure valve
US12016687B2 (en) 2020-06-23 2024-06-25 The Board Of Trustees Of The University Of Illinois Implantable probes and methods of fabrication

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4531056A (en) * 1983-04-20 1985-07-23 Yale University Method and apparatus for the mass spectrometric analysis of solutions
EP0225717A1 (en) * 1985-11-01 1987-06-16 Varian Associates, Inc. High current mass spectrometer using space charge lens
WO1997004297A1 (en) * 1995-07-21 1997-02-06 Northeastern University Microscale fluid handling system
US5608217A (en) * 1994-03-10 1997-03-04 Bruker-Franzen Analytik Gmbh Electrospraying method for mass spectrometric analysis
WO1997022825A1 (en) * 1995-12-18 1997-06-26 Neukermans Armand P Microfluidic valve and integrated microfluidic system
EP0886143A1 (en) * 1998-03-27 1998-12-23 SYNSORB Biotech Inc. Apparatus for screening compound libraries

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4531056A (en) * 1983-04-20 1985-07-23 Yale University Method and apparatus for the mass spectrometric analysis of solutions
EP0225717A1 (en) * 1985-11-01 1987-06-16 Varian Associates, Inc. High current mass spectrometer using space charge lens
US5608217A (en) * 1994-03-10 1997-03-04 Bruker-Franzen Analytik Gmbh Electrospraying method for mass spectrometric analysis
WO1997004297A1 (en) * 1995-07-21 1997-02-06 Northeastern University Microscale fluid handling system
WO1997022825A1 (en) * 1995-12-18 1997-06-26 Neukermans Armand P Microfluidic valve and integrated microfluidic system
EP0886143A1 (en) * 1998-03-27 1998-12-23 SYNSORB Biotech Inc. Apparatus for screening compound libraries

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FIGEYS D ET AL: "A MICROFABRICATED DEVICE FOR RAPID PROTEIN IDENTIFICATION BY MICROELECTROSPRAY ION TRAP MASS SPECTROMETRY", ANALYTICAL CHEMISTRY,US,AMERICAN CHEMICAL SOCIETY. COLUMBUS, vol. 69, no. 16, 15 August 1997 (1997-08-15), pages 3153 - 3160, XP000699502, ISSN: 0003-2700 *
LINTEL VAN H T G ET AL: "A PIEZOELECTRIC MICROPUMP BASED ON MICROMACHINING OF SILICON", SENSORS AND ACTUATORS,CH,ELSEVIER SEQUOIA S.A. LAUSANNE, vol. 15, 1 October 1988 (1988-10-01), pages 153 - 167, XP000608581 *
QIFENG XUE ET AL: "MULTICHANNEL MICROCHIP ELECTROSPRAY MASS SPECTROMETRY", ANALYTICAL CHEMISTRY, vol. 69, no. 3, 1 February 1997 (1997-02-01), pages 426 - 430, XP000682290 *
WANG X-Q ET AL: "POLYMER-BASED ELECTROSPRAY CHIPS FOR MASS SPECTROMETRY", PROCEEDINGS OF THE TWELFTH IEEE INTERNATIONAL WORKSHOP ON MICRO ELECTRO MECHANICAL SYSTEMS - MEMS, ORLANDO, US, 17-21 JAN. 1999, PAGES 523-528, XP000830802 *

Also Published As

Publication number Publication date
WO2000050880A2 (en) 2000-08-31
GB9904038D0 (en) 1999-04-14

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122 Ep: pct application non-entry in european phase