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WO2000040709A3 - PROCEDE PERMETTANT DE FAIRE CODER AUX PLANTES ET CELLULES DE PLANTES DE LA LYSINE OU DE LA METHIONINE OU DU TRYPTOPHANE OU DE LA THREONINE A DES CODONS D'ARNm DIFFERENTS DE CEUX SPECIFIANT NORMALEMENT CES ACIDES AMINES PARTICULIERS DURANT LA SYNTHESE PROTEIQUE - Google Patents

PROCEDE PERMETTANT DE FAIRE CODER AUX PLANTES ET CELLULES DE PLANTES DE LA LYSINE OU DE LA METHIONINE OU DU TRYPTOPHANE OU DE LA THREONINE A DES CODONS D'ARNm DIFFERENTS DE CEUX SPECIFIANT NORMALEMENT CES ACIDES AMINES PARTICULIERS DURANT LA SYNTHESE PROTEIQUE Download PDF

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Publication number
WO2000040709A3
WO2000040709A3 PCT/IB1999/002125 IB9902125W WO0040709A3 WO 2000040709 A3 WO2000040709 A3 WO 2000040709A3 IB 9902125 W IB9902125 W IB 9902125W WO 0040709 A3 WO0040709 A3 WO 0040709A3
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WO
WIPO (PCT)
Prior art keywords
plants
trna
binds
express
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB1999/002125
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English (en)
Other versions
WO2000040709A2 (fr
Inventor
William R Folk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU41354/00A priority Critical patent/AU4135400A/en
Publication of WO2000040709A2 publication Critical patent/WO2000040709A2/fr
Publication of WO2000040709A3 publication Critical patent/WO2000040709A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé permettant de faire coder aux cellules de plantes, ou aux tissus de plantes, de la lysine, ou de la méthionine, ou du tryptophane, ou de la thréonine, à des codons d'ARNm différents de ceux spécifiant normalement ces acides aminés particuliers durant la synthèse protéique. Ce procédé consiste: i) à préparer un ADN à exprimer un ARNt chargé en lysine qui se lie, lors de la synthèse protéique, à des codons d'ARNm autres que AAA ou AAG, ou à préparer un ADN qui exprime un ARNt chargé en méthionine qui se lie à des codons d'ARNm autres que AUG, ou à préparer un ADN qui exprime un ARNt chargé en tryptophane qui se lie à des codons d'ARNm autres que UGG, ou à préparer un ADN qui exprime un ARNt chargé en thréonine qui se lie à des codons d'ARNm autres que ACU, ACC, ACA, ACG, ii) et à introduire un ou plusieurs desdits ADN dans des protoplastes de plantes, dans des cellules de plantes, ou dans des plantes.
PCT/IB1999/002125 1998-12-24 1999-12-24 PROCEDE PERMETTANT DE FAIRE CODER AUX PLANTES ET CELLULES DE PLANTES DE LA LYSINE OU DE LA METHIONINE OU DU TRYPTOPHANE OU DE LA THREONINE A DES CODONS D'ARNm DIFFERENTS DE CEUX SPECIFIANT NORMALEMENT CES ACIDES AMINES PARTICULIERS DURANT LA SYNTHESE PROTEIQUE Ceased WO2000040709A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU41354/00A AU4135400A (en) 1998-12-24 1999-12-24 Process for causing plant cells and plants to code lysine or methionine or tryptophan or threonine at mrna codons other than those normally specifying these particular amino acids during protein synthesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22037598A 1998-12-24 1998-12-24
US09/220,375 1998-12-24

Publications (2)

Publication Number Publication Date
WO2000040709A2 WO2000040709A2 (fr) 2000-07-13
WO2000040709A3 true WO2000040709A3 (fr) 2000-11-16

Family

ID=22823308

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1999/002125 Ceased WO2000040709A2 (fr) 1998-12-24 1999-12-24 PROCEDE PERMETTANT DE FAIRE CODER AUX PLANTES ET CELLULES DE PLANTES DE LA LYSINE OU DE LA METHIONINE OU DU TRYPTOPHANE OU DE LA THREONINE A DES CODONS D'ARNm DIFFERENTS DE CEUX SPECIFIANT NORMALEMENT CES ACIDES AMINES PARTICULIERS DURANT LA SYNTHESE PROTEIQUE

Country Status (2)

Country Link
AU (1) AU4135400A (fr)
WO (1) WO2000040709A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11312972B2 (en) 2016-11-16 2022-04-26 Cellectis Methods for altering amino acid content in plants through frameshift mutations

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998005789A2 (fr) * 1996-08-06 1998-02-12 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Expression genetique controlee dans les plantes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998005789A2 (fr) * 1996-08-06 1998-02-12 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Expression genetique controlee dans les plantes

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BETZNER, A.S., ET AL.: "transfer RNA-mediated suppression of amber stop codons in transgenic Arabidopsis thaliana", THE PLANT JOURNAL, vol. 11, no. 3, 1997, pages 587 - 595, XP002146600 *
CHEN ZHIHONG ET AL: "Nonsense and missense translational suppression in plant cells mediated by tRNALys.", PLANT MOLECULAR BIOLOGY, vol. 36, no. 1, 1 January 1998 (1998-01-01), pages 163 - 170, XP002146596, ISSN: 0167-4412 *
CHOISNE, N., ET AL.: "transactivation of a target gene using a suppressor tRNA in transgenic tobacco plants", THE PLANT JOURNAL, vol. 11, no. 3, 1 March 1997 (1997-03-01), pages 597 - 604, XP002070646 *
DATABASE EMBL SEQUENCE LIBRARY 23 September 1996 (1996-09-23), CHEN,Z.,ET AL.: "nonsense and missense translational suppression in plant cells mediated by tRNA(Lys)", XP002146601 *
FRANKLIN, S., ET AL.: "CONSTRUCTION AND EXPRESSION OF NONSENSE SUPPRESSOR tRNAs WHICH FUNCTION IN PLANT CELLS", THE PLANT JOURNAL, vol. 2, no. 4, 1992, pages 583 - 588, XP002146599 *
ULMASOV BARBARA ET AL: "Analysis of the role of 5' and 3' flanking sequence elements upon in vivo expression of the plant tRNA-Trp genes.", PLANT CELL, vol. 7, no. 10, 1995, pages 1723 - 1734, XP002146595, ISSN: 1040-4651 *
ULMASOV BARBARA ET AL: "Identity elements and aminoacylation of plant tRNATrp.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 22, 15 November 1998 (1998-11-15), pages 5139 - 5141, XP002146597, ISSN: 0305-1048 *
ULMASOV BARBARA ET AL: "Regulated expression of plant tRNA genes by the prokaryotic tet and lac repressors.", PLANT MOLECULAR BIOLOGY, vol. 35, no. 4, 1997, pages 417 - 424, XP002146598, ISSN: 0167-4412 *

Also Published As

Publication number Publication date
AU4135400A (en) 2000-07-24
WO2000040709A2 (fr) 2000-07-13

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