[go: up one dir, main page]

WO1999034220A3 - Real-time monitoring of an analyte by chromatography using an on-line assay - Google Patents

Real-time monitoring of an analyte by chromatography using an on-line assay Download PDF

Info

Publication number
WO1999034220A3
WO1999034220A3 PCT/US1998/026172 US9826172W WO9934220A3 WO 1999034220 A3 WO1999034220 A3 WO 1999034220A3 US 9826172 W US9826172 W US 9826172W WO 9934220 A3 WO9934220 A3 WO 9934220A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell culture
analyte
culture fluid
protein
loading
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/026172
Other languages
French (fr)
Other versions
WO1999034220A2 (en
Inventor
Gregory S Blank
Robert Lee Fahrner
Martin Vanderlaan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Priority to EP98965982A priority Critical patent/EP1044378A2/en
Priority to AU21990/99A priority patent/AU2199099A/en
Publication of WO1999034220A2 publication Critical patent/WO1999034220A2/en
Publication of WO1999034220A3 publication Critical patent/WO1999034220A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The presence of a contaminating analyte in a protein mixture may obscure detection of a protein of interest. In addition, antibody breakthrough is obscured by flow-through of contaminant proteins when loading cell culture fluid onto affinity columns. An on-line chromatographic assay described herein allows real-time measurement of contaminating analyte during purification or antibody breakthrough during loading and collection of a protein of interest in preparative chromatography. Breakthrough curves are generated allowing the user to end loading of cell culture fluid and/or to end effluent collection for maximization of efficient purification and preventing waste of cell culture fluid.
PCT/US1998/026172 1997-12-31 1998-12-09 Real-time monitoring of an analyte by chromatography using an on-line assay Ceased WO1999034220A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP98965982A EP1044378A2 (en) 1997-12-31 1998-12-09 Real-time monitoring of an analyte by chromatography using an on-line assay
AU21990/99A AU2199099A (en) 1997-12-31 1998-12-09 Real-time monitoring of an analyte by chromatography using an on-line assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7015097P 1997-12-31 1997-12-31
US60/070,150 1997-12-31

Publications (2)

Publication Number Publication Date
WO1999034220A2 WO1999034220A2 (en) 1999-07-08
WO1999034220A3 true WO1999034220A3 (en) 1999-09-02

Family

ID=22093458

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/026172 Ceased WO1999034220A2 (en) 1997-12-31 1998-12-09 Real-time monitoring of an analyte by chromatography using an on-line assay

Country Status (3)

Country Link
EP (1) EP1044378A2 (en)
AU (1) AU2199099A (en)
WO (1) WO1999034220A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106353440A (en) * 2015-07-13 2017-01-25 帕尔公司 Optimizing operating binding capacity for a multiple column chromatography process

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2379823A1 (en) 1999-07-16 2001-01-25 Human Genome Sciences, Inc. Real-time, in situ biomanufacturing process monitoring and control in response to ir spectroscopy
US8029743B2 (en) 2007-09-19 2011-10-04 General Electric Company Microfluidic device with vertical injection aperture
US7740747B2 (en) 2007-12-28 2010-06-22 General Electric Company Injection method for microfluidic chips
US7740748B2 (en) 2008-10-27 2010-06-22 General Electric Company Electrophoresis system and method
US7927476B2 (en) 2008-12-22 2011-04-19 General Electric Company Injection method for microfluidic chips
DE102014010353B4 (en) 2014-07-10 2024-09-12 Sartorius Stedim Biotech Gmbh Device, use of this device and method for substance separation with improved utilization of the capacity of chromatographic media
EP3683579B1 (en) 2016-04-14 2024-10-23 Lonza Ltd Methods for the detection of host cell proteins
CN119881293A (en) 2018-03-21 2025-04-25 沃特世科技公司 Non-antibody-based high affinity sample preparation, adsorbents, devices and methods

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993007168A2 (en) * 1991-09-30 1993-04-15 Perseptive Biosystems, Inc. Protein chromatography system
US5234586A (en) * 1991-03-28 1993-08-10 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
WO1993020449A1 (en) * 1992-03-27 1993-10-14 Perseptive Biosystems, Inc. Rapid hypersensitive flowthrough immunodetection system
WO1996040398A1 (en) * 1995-06-07 1996-12-19 Biosepra Inc. On line detection of a desired solute in an effluent stream using fluorescence spectroscopy

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234586A (en) * 1991-03-28 1993-08-10 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
WO1993007168A2 (en) * 1991-09-30 1993-04-15 Perseptive Biosystems, Inc. Protein chromatography system
WO1993020449A1 (en) * 1992-03-27 1993-10-14 Perseptive Biosystems, Inc. Rapid hypersensitive flowthrough immunodetection system
WO1996040398A1 (en) * 1995-06-07 1996-12-19 Biosepra Inc. On line detection of a desired solute in an effluent stream using fluorescence spectroscopy

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
H. A. CHASE: "Rapid chromatographic monitoring of bioprocesses", BIOSENSORS, vol. 2, 1986, ISSN 0265-928X, pages 269 - 286, XP002105368 *
LEVISON P R ET AL: "Influence of flow-rate on the chromatographic performance of agarose- and cellulose-based anion-exchange media", JOURNAL OF CHROMATOGRAPHY A, vol. 734, no. 1, 17 May 1996 (1996-05-17), pages 137-143, XP004039088 *
N.B. AFEYAN: "Automated real-time immunoassay of biomolecules", NATURE., vol. 358, 13 August 1992 (1992-08-13), LONDON GB, pages 603 - 604, XP002105367 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106353440A (en) * 2015-07-13 2017-01-25 帕尔公司 Optimizing operating binding capacity for a multiple column chromatography process

Also Published As

Publication number Publication date
EP1044378A2 (en) 2000-10-18
WO1999034220A2 (en) 1999-07-08
AU2199099A (en) 1999-07-19

Similar Documents

Publication Publication Date Title
Carlucci Analysis of fluoroquinolones in biological fluids by high-performance liquid chromatography
MY129171A (en) Chromatographic assay device and method for detecting the presence of an analyte in a blood sample
Van Emon et al. Bioseparation and bioanalytical techniques in environmental monitoring
WO1998035232A3 (en) Electrochemical detection of specific binding
AU2855797A (en) Analytical system with means for detecting too small sample volumes
DE69434038D1 (en) SIMPLE IMMUNOCHEMICAL SEMIQUANTITATIVE DETERMINATION METHOD AND DEVICE
AU2001271501A1 (en) Opposable-element chromatographic assay device for detection of analytes in whole blood samples
AU5964494A (en) Assays and kits for detecting analytes in the presence of cross-reacting substances
WO1999045374A3 (en) Purification and detection processes using reversible affinity electrophoresis
ZA978630B (en) Method for collecting samples of liquid specimens for analytical testing.
GB9212416D0 (en) Reversible binding substances
AU3156489A (en) Solid-phase analytical device and method for using same
CA2130072A1 (en) Method of detecting a pathogen using a virus
WO1999034220A3 (en) Real-time monitoring of an analyte by chromatography using an on-line assay
Irth et al. Strategies for on-line coupling of immunoassays to high-performance liquid chromatography
Steinmann et al. Feasibility study of a drug immunoassay based on micellar electrokinetic capillary chromatography with laser induced fluorescence detection: Determination of theophylline in serum
Regnier et al. Future potential of targeted component analysis by multidimensional liquid chromatography-mass spectrometry
Law et al. Confirmation of cannabis use by the analysis of Δ9-tetrahydrocannabinol metabolites in blood and urine by combined HPLC and RIA
Kato et al. A new packing for separation of DNA restriction fragments by high performance liquid chromatography
SE9502024D0 (en) Pathogen assay method
Williams et al. Extended life for blood serum analysis columns using dual zone chromatographic materials
Ohlson et al. High-performance liquid affinity chromatographic separation of mouse monoclonal antibodies with protein A silica
RU94032808A (en) Process of determination of acetophenon in air
EP1234183A4 (en) EIA FOR MONITORING i LEGIONELLA PNEUMOPHILA /i PRESENCE IN WATER SAMPLES
WO2002014869A3 (en) Assay method and device for detecting the presence and concentration of analytes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
NENP Non-entry into the national phase

Ref country code: KR

WWE Wipo information: entry into national phase

Ref document number: 1998965982

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1998965982

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 1998965982

Country of ref document: EP