[go: up one dir, main page]

WO1999034003A3 - Proteases provenant d'organismes a gram positif - Google Patents

Proteases provenant d'organismes a gram positif Download PDF

Info

Publication number
WO1999034003A3
WO1999034003A3 PCT/US1998/027020 US9827020W WO9934003A3 WO 1999034003 A3 WO1999034003 A3 WO 1999034003A3 US 9827020 W US9827020 W US 9827020W WO 9934003 A3 WO9934003 A3 WO 9934003A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
gram positive
provides
proteases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/027020
Other languages
English (en)
Other versions
WO1999034003A2 (fr
Inventor
David A Estell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Priority to US09/554,998 priority Critical patent/US6599731B1/en
Priority to AU19301/99A priority patent/AU1930199A/en
Priority to EP98964108A priority patent/EP1042490A2/fr
Publication of WO1999034003A2 publication Critical patent/WO1999034003A2/fr
Publication of WO1999034003A3 publication Critical patent/WO1999034003A3/fr
Anticipated expiration legal-status Critical
Priority to US11/013,992 priority patent/US20050101504A1/en
Priority to US11/014,361 priority patent/US7098021B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne l'identification d'une nouvelle métalloprotéase dans des micro-organismes à Gram positif. Elle concerne également les séquences d'acides nucléiques et d'acides aminés de cette métalloprotéase; des cellules hôtes présentant une mutation ou une délétion d'une partie ou de la totalité du gène codant la métalloprotéase; des cellules hôtes contenant, de plus, un acide nucléique codant des protéines hétérologues souhaitées, telles que des enzymes; des compositions de nettoyage, des aliments pour animaux et des compositions utilisées pour traiter un textile, qui contiennent cette métalloprotéase.
PCT/US1998/027020 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif Ceased WO1999034003A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US09/554,998 US6599731B1 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
AU19301/99A AU1930199A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
EP98964108A EP1042490A2 (fr) 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif
US11/013,992 US20050101504A1 (en) 1997-12-30 2004-12-16 Proteases from gram positive organisms
US11/014,361 US7098021B2 (en) 1997-12-30 2004-12-16 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727464.1A GB9727464D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727464.1 1997-12-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09554998 A-371-Of-International 1998-12-17
US10/460,718 Division US6872807B2 (en) 1997-12-30 2003-06-12 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999034003A2 WO1999034003A2 (fr) 1999-07-08
WO1999034003A3 true WO1999034003A3 (fr) 1999-11-18

Family

ID=10824308

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027020 Ceased WO1999034003A2 (fr) 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif

Country Status (4)

Country Link
EP (1) EP1042490A2 (fr)
AU (1) AU1930199A (fr)
GB (1) GB9727464D0 (fr)
WO (1) WO1999034003A2 (fr)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
ES2337131T3 (es) 2001-12-07 2010-04-21 Novozymes A/S Polipeptidos con actividad proteasa y acidos nucleicos que codifican los mismos.
US20110306139A1 (en) * 2009-02-27 2011-12-15 Novozymes A/S Mutant Cells Suitable For Recombinant Polypeptide Production
US10533165B2 (en) 2013-12-13 2020-01-14 Danisco Us Inc Serine proteases of the bacillus gibsonii-clade
TR201906371T4 (tr) 2013-12-13 2019-05-21 Danisco Inc Bacillus türlerinin serin proteazları.
CA2932498C (fr) 2013-12-16 2023-03-14 E. I. Du Pont De Nemours And Company Utilisation de poly(ethers d'alpha-1,3-glucane) en tant que modificateurs de viscosite
CN106029700B (zh) 2013-12-18 2019-04-12 纳幕尔杜邦公司 阳离子聚α-1，3-葡聚糖醚
WO2015123323A1 (fr) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucanes utilisables en vue de la modification de la viscosité
ES2734726T3 (es) 2014-03-11 2019-12-11 Du Pont Poli alfa-1,2-glucano oxidado como reforzante de detergente
CN106170546A (zh) 2014-03-21 2016-11-30 丹尼斯科美国公司 芽孢杆菌属的丝氨酸蛋白酶
WO2015195777A1 (fr) 2014-06-19 2015-12-23 E. I. Du Pont De Nemours And Company Compositions contenant un ou plusieurs composés d'éther de poly alpha-1,3-glucane
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
US20170233710A1 (en) 2014-10-17 2017-08-17 Danisco Us Inc. Serine proteases of bacillus species
WO2016069544A1 (fr) 2014-10-27 2016-05-06 Danisco Us Inc. Sérines protéases
DK3212781T3 (da) 2014-10-27 2019-12-16 Danisco Us Inc Serinproteaser
CN107148472A (zh) 2014-10-27 2017-09-08 丹尼斯科美国公司 芽孢杆菌属物种的丝氨酸蛋白酶
WO2016069569A2 (fr) 2014-10-27 2016-05-06 Danisco Us Inc. Sérine protéases
WO2016069552A1 (fr) 2014-10-27 2016-05-06 Danisco Us Inc. Sérines protéases
WO2016106011A1 (fr) 2014-12-23 2016-06-30 E. I. Du Pont De Nemours And Company Cellulose produite par voie enzymatique
US20180112204A1 (en) 2015-05-13 2018-04-26 Danisco Us Inc. AprL-CLADE PROTEASE VARIANTS AND USES THEREOF
EP3310911B1 (fr) 2015-06-17 2023-03-15 Danisco US Inc. Protéases à sérines du clade du bacillus gibsonii
US20190153417A1 (en) 2015-11-05 2019-05-23 Danisco Us Inc Paenibacillus sp. mannanases
US20180320158A1 (en) 2015-11-05 2018-11-08 Danisco Us Inc. Paenibacillus and bacillus spp. mannanases
WO2017083226A1 (fr) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Compositions de fibre de glucane à utiliser dans l'entretien du linge et l'entretien de tissu
EP3374401B1 (fr) 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Compositions de fibres de glucane utiles pour la lessive et l'entretien des tissus
WO2017083228A1 (fr) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Compositions de fibres de glucane utiles pour la lessive et l'entretien des tissus
US20180362946A1 (en) 2015-12-18 2018-12-20 Danisco Us Inc. Polypeptides with endoglucanase activity and uses thereof
CA3026064A1 (fr) 2016-05-31 2017-12-07 Danisco Us Inc. Variants de protease et leurs utilisations
MX2018015559A (es) 2016-06-17 2019-06-06 Danisco Us Inc Variantes de proteasa y sus usos.
WO2018085524A2 (fr) 2016-11-07 2018-05-11 Danisco Us Inc Composition détergente pour le linge
WO2018118950A1 (fr) 2016-12-21 2018-06-28 Danisco Us Inc. Sérine-protéases du clade du bacillus gibsonii
EP3559227B1 (fr) 2016-12-21 2025-02-19 Danisco US Inc. Variants de protéases et leurs utilisations
WO2018169750A1 (fr) 2017-03-15 2018-09-20 Danisco Us Inc Sérine protéases de type trypsine et leurs utilisations
CN111373039A (zh) 2017-11-29 2020-07-03 丹尼斯科美国公司 具有改善的稳定性的枯草杆菌蛋白酶变体
EP3810767A1 (fr) 2018-06-19 2021-04-28 Danisco US Inc. Variantes de subtilisine
WO2019245705A1 (fr) 2018-06-19 2019-12-26 Danisco Us Inc Variants de subtilisine
US20220033737A1 (en) 2018-09-27 2022-02-03 Danisco Us Inc Compositions for medical instrument cleaning
EP3887515A1 (fr) 2018-11-28 2021-10-06 Danisco US Inc. Variants de subtilisine à stabilité améliorée
EP3976776A1 (fr) 2019-05-24 2022-04-06 Danisco US Inc. Variants de subtilisine et procédés d'utilisation
CN114174486A (zh) 2019-06-06 2022-03-11 丹尼斯科美国公司 用于清洁的方法和组合物
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
WO2022165107A1 (fr) 2021-01-29 2022-08-04 Danisco Us Inc Compositions pour le nettoyage et procédés associés
CN117616120A (zh) 2021-06-30 2024-02-27 丹尼斯科美国公司 变体脂肪酶及其用途
US20240384205A1 (en) 2021-09-03 2024-11-21 Danisco Us Inc. Laundry compositions for cleaning
WO2023114936A2 (fr) 2021-12-16 2023-06-22 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
EP4448749A2 (fr) 2021-12-16 2024-10-23 Danisco US Inc. Variants de subtilisine et procédés d'utilisation
CN118715318A (zh) 2021-12-16 2024-09-27 丹尼斯科美国公司 枯草杆菌蛋白酶变体及其用途
WO2023168234A1 (fr) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes et compositions enzymatiques pour le nettoyage
EP4544016A1 (fr) 2022-06-21 2025-04-30 Danisco US Inc. Procédés et compositions de nettoyage comprenant un polypeptide ayant une activité de thermolysine
EP4581138A1 (fr) 2022-09-02 2025-07-09 Danisco US Inc. Variants de subtilisine et procédés associés
JP2025530749A (ja) 2022-09-02 2025-09-17 ダニスコ・ユーエス・インク 洗剤組成物及びそれに関連する方法
CN119816592A (zh) 2022-09-02 2025-04-11 丹尼斯科美国公司 甘露聚糖酶变体和使用方法
CN120303400A (zh) 2022-11-09 2025-07-11 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
CN120677237A (zh) 2023-02-01 2025-09-19 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
CN120712348A (zh) 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2024191711A1 (fr) 2023-03-16 2024-09-19 Nutrition & Biosciences USA 4, Inc. Extraits fermentés de brevibacillus pour le nettoyage et la lutte contre les mauvaises odeurs et leur utilisation
WO2025071996A1 (fr) 2023-09-28 2025-04-03 Danisco Us Inc. Variant d'enzymes cutinases à solubilité améliorée et leurs utilisations
WO2025085351A1 (fr) 2023-10-20 2025-04-24 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus
FR2728905A1 (fr) * 1994-12-29 1996-07-05 Rhone Poulenc Nutrition Animal Nouvelle acide amine amidohydrolase, sequence nuclotidique correspondant et leurs utilisations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus
FR2728905A1 (fr) * 1994-12-29 1996-07-05 Rhone Poulenc Nutrition Animal Nouvelle acide amine amidohydrolase, sequence nuclotidique correspondant et leurs utilisations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002106131, Database accession no. Z99120 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002106132, Database accession no. G70017 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
EP1042490A2 (fr) 2000-10-11
GB9727464D0 (en) 1998-02-25
WO1999034003A2 (fr) 1999-07-08
AU1930199A (en) 1999-07-19

Similar Documents

Publication Publication Date Title
WO1999033960A3 (fr) Proteases de germes gram positifs
WO1999034003A3 (fr) Proteases provenant d'organismes a gram positif
WO1999014341A3 (fr) Proteases extraites d'organismes gram positif
EP0516200B1 (fr) Compositions de détergents contenant des enzymes stabilisés
WO2001079463A3 (fr) Acides nucleiques codant pour des polypeptides ayant une activite haloperoxydase
WO2001079462A3 (fr) Acides nucleiques codant pour des polypeptides presentant une activite haloperoxydase
WO2001064898A3 (fr) Genes codant pour des enzymes de denitrification
EP0994190A3 (fr) Séquence d'ADN conferant aux bactéries une résistance à l'homosérine et son utilisation
WO1999014342A1 (fr) Proteases d'organismes gram positifs
KR20120044360A (ko) 서브틸라제 변이체
ATE450605T1 (de) Phytase enzyme, dafür kodierende nukleinsäuren, und solche enthaltende vectoren und wirtszellen
WO1998056927A3 (fr) Acides nucleiques codant des polypeptides a fonction protease
HU9302252D0 (en) New oxalate oxidases and their applications
WO1999003984A3 (fr) Proteases pour organismes gram-positifs
CA2253006A1 (fr) Gene hydroxylase de beta-carotene
WO1999034001A3 (fr) Proteases provenant d'organismes a gram positif
AU5727099A (en) A 2,6-beta-d-fructan hydrolase enzyme and processes for using the enzyme
WO1999033959A3 (fr) Proteases provenant d'organismes a gram positif
US20050059112A1 (en) Proteases from gram-positive organisms
US7329525B2 (en) Serine proteases from gram-positive microorganisms
WO1999034002A3 (fr) Proteases provenant d'organismes a gram positif
Estell Proteases from gram positive organisms
WO1999004016A3 (fr) Proteases pour germes gram positif
WO1999004007A8 (fr) Augmentation de la production de proteines dans les micro-organismes gram-positifs
US20050101504A1 (en) Proteases from gram positive organisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AT AU AZ BA BB BG BR BY CA CH CN CU CZ CZ DE DE DK DK EE EE ES FI FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AT AU AZ BA BB BG BR BY CA CH CN CU CZ CZ DE DE DK DK EE EE ES FI FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 09554998

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: KR

WWE Wipo information: entry into national phase

Ref document number: 1998964108

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1998964108

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 1998964108

Country of ref document: EP