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WO1999033960A3 - Proteases from gram positive organisms - Google Patents

Proteases from gram positive organisms Download PDF

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Publication number
WO1999033960A3
WO1999033960A3 PCT/US1998/027040 US9827040W WO9933960A3 WO 1999033960 A3 WO1999033960 A3 WO 1999033960A3 US 9827040 W US9827040 W US 9827040W WO 9933960 A3 WO9933960 A3 WO 9933960A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
gram positive
provides
proteases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/027040
Other languages
French (fr)
Other versions
WO1999033960A2 (en
Inventor
David A Estell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US09/554,999 priority Critical patent/US6465186B1/en
Application filed by Genencor International Inc filed Critical Genencor International Inc
Priority to AU18327/99A priority patent/AU1832799A/en
Priority to EP98963276A priority patent/EP1042487A2/en
Publication of WO1999033960A2 publication Critical patent/WO1999033960A2/en
Publication of WO1999033960A3 publication Critical patent/WO1999033960A3/en
Anticipated expiration legal-status Critical
Priority to US10/188,586 priority patent/US6794179B2/en
Priority to US10/925,821 priority patent/US7078372B2/en
Priority to US10/925,708 priority patent/US7070990B2/en
Priority to US10/926,197 priority patent/US7070986B2/en
Priority to US10/925,652 priority patent/US7070819B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Animal Husbandry (AREA)
  • Textile Engineering (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
PCT/US1998/027040 1997-12-30 1998-12-17 Proteases from gram positive organisms Ceased WO1999033960A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
US09/554,999 US6465186B1 (en) 1997-12-30 1998-12-01 Proteases from gram positive organisms
AU18327/99A AU1832799A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
EP98963276A EP1042487A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
US10/188,586 US6794179B2 (en) 1997-12-30 2002-07-02 Proteases from gram positive organisms
US10/925,821 US7078372B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms
US10/925,708 US7070990B2 (en) 1997-12-30 2004-08-24 Proteases from GRAM positive organisms
US10/926,197 US7070986B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms
US10/925,652 US7070819B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727471.6A GB9727471D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727471.6 1997-12-30

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09/554,999 Continuation US6465186B1 (en) 1997-12-30 1998-12-01 Proteases from gram positive organisms
US09554999 A-371-Of-International 1998-12-17
US10/188,586 Division US6794179B2 (en) 1997-12-30 2002-07-02 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999033960A2 WO1999033960A2 (en) 1999-07-08
WO1999033960A3 true WO1999033960A3 (en) 2000-03-02

Family

ID=10824314

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027040 Ceased WO1999033960A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms

Country Status (4)

Country Link
EP (1) EP1042487A2 (en)
AU (1) AU1832799A (en)
GB (1) GB9727471D0 (en)
WO (1) WO1999033960A2 (en)

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AU3739799A (en) * 1997-12-09 1999-08-09 Regents Of The University Of California, The Block polymer processing for mesostructured inorganic oxide materials
GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
EP1546342A2 (en) * 2002-09-20 2005-06-29 Cym1P A/S Methods for increasing the production of a recombinant polypeptide from a host cell
US10533165B2 (en) 2013-12-13 2020-01-14 Danisco Us Inc Serine proteases of the bacillus gibsonii-clade
TR201906371T4 (en) 2013-12-13 2019-05-21 Danisco Inc Serine proteases of Bacillus species.
CA2932498C (en) 2013-12-16 2023-03-14 E. I. Du Pont De Nemours And Company Use of poly alpha-1,3-glucan ethers as viscosity modifiers
CN106029700B (en) 2013-12-18 2019-04-12 纳幕尔杜邦公司 The poly- α -1 of cation, 3- glucan ether
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
ES2734726T3 (en) 2014-03-11 2019-12-11 Du Pont Oxidized alpha-1,2-glucan poly as a detergent booster
CN106170546A (en) 2014-03-21 2016-11-30 丹尼斯科美国公司 Bacillus serine protease
WO2015195777A1 (en) 2014-06-19 2015-12-23 E. I. Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
US20170233710A1 (en) 2014-10-17 2017-08-17 Danisco Us Inc. Serine proteases of bacillus species
CN107148472A (en) 2014-10-27 2017-09-08 丹尼斯科美国公司 Serine proteases from Bacillus sp.
WO2016069544A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
DK3212781T3 (en) 2014-10-27 2019-12-16 Danisco Us Inc serine proteases
WO2016069569A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
WO2016069552A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
WO2016106011A1 (en) 2014-12-23 2016-06-30 E. I. Du Pont De Nemours And Company Enzymatically produced cellulose
US20180112204A1 (en) 2015-05-13 2018-04-26 Danisco Us Inc. AprL-CLADE PROTEASE VARIANTS AND USES THEREOF
EP3310911B1 (en) 2015-06-17 2023-03-15 Danisco US Inc. Bacillus gibsonii-clade serine proteases
US20180320158A1 (en) 2015-11-05 2018-11-08 Danisco Us Inc. Paenibacillus and bacillus spp. mannanases
US20190153417A1 (en) 2015-11-05 2019-05-23 Danisco Us Inc Paenibacillus sp. mannanases
EP3374401B1 (en) 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
WO2017083228A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
WO2017083226A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
US20180362946A1 (en) 2015-12-18 2018-12-20 Danisco Us Inc. Polypeptides with endoglucanase activity and uses thereof
CA3026064A1 (en) 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
MX2018015559A (en) 2016-06-17 2019-06-06 Danisco Us Inc Protease variants and uses thereof.
WO2018085524A2 (en) 2016-11-07 2018-05-11 Danisco Us Inc Laundry detergent composition
WO2018118950A1 (en) 2016-12-21 2018-06-28 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
EP3559227B1 (en) 2016-12-21 2025-02-19 Danisco US Inc. Protease variants and uses thereof
WO2018169750A1 (en) 2017-03-15 2018-09-20 Danisco Us Inc Trypsin-like serine proteases and uses thereof
CN111373039A (en) 2017-11-29 2020-07-03 丹尼斯科美国公司 Subtilisin variants having improved stability
WO2019245705A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
EP3810767A1 (en) 2018-06-19 2021-04-28 Danisco US Inc. Subtilisin variants
US20220033737A1 (en) 2018-09-27 2022-02-03 Danisco Us Inc Compositions for medical instrument cleaning
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
EP3976776A1 (en) 2019-05-24 2022-04-06 Danisco US Inc. Subtilisin variants and methods of use
CN114174486A (en) 2019-06-06 2022-03-11 丹尼斯科美国公司 Methods and compositions for cleaning
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
WO2022165107A1 (en) 2021-01-29 2022-08-04 Danisco Us Inc Compositions for cleaning and methods related thereto
CN117616120A (en) 2021-06-30 2024-02-27 丹尼斯科美国公司 Variant lipases and uses thereof
US20240384205A1 (en) 2021-09-03 2024-11-21 Danisco Us Inc. Laundry compositions for cleaning
WO2023114936A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448749A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and methods of use
CN118715318A (en) 2021-12-16 2024-09-27 丹尼斯科美国公司 Subtilisin variants and uses thereof
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
EP4544016A1 (en) 2022-06-21 2025-04-30 Danisco US Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
EP4581138A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Subtilisin variants and methods related thereto
CN119816592A (en) 2022-09-02 2025-04-11 丹尼斯科美国公司 Mannanase variants and methods of use
JP2025530749A (en) 2022-09-02 2025-09-17 ダニスコ・ユーエス・インク Detergent compositions and related methods
CN120303400A (en) 2022-11-09 2025-07-11 丹尼斯科美国公司 Subtilisin variants and methods of use
CN120677237A (en) 2023-02-01 2025-09-19 丹尼斯科美国公司 Subtilisin variants and methods of use
CN120712348A (en) 2023-03-06 2025-09-26 丹尼斯科美国公司 Subtilisin variants and methods of use
WO2024191711A1 (en) 2023-03-16 2024-09-19 Nutrition & Biosciences USA 4, Inc. Brevibacillus fermentate extracts for cleaning and malodor control and use thereof
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002115981, Database accession no. Z99112 *
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), XP002115982, Database accession no. G69885 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
EP1042487A2 (en) 2000-10-11
WO1999033960A2 (en) 1999-07-08
GB9727471D0 (en) 1998-02-25
AU1832799A (en) 1999-07-19

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