WO1999020287A1

WO1999020287A1 - The use of an aqueous solution in the preparation of a medicament for use in the treatment of live animals

Info

Publication number: WO1999020287A1
Application number: PCT/US1998/022372
Authority: WO
Inventors: Gilbert Theo Hinze
Original assignee: DAVIS JOANNE T; Radical Waters IP Pty Ltd
Current assignee: DAVIS JOANNE T; Radical Waters IP Pty Ltd
Priority date: 1997-10-23
Filing date: 1998-10-23
Publication date: 1999-04-29
Anticipated expiration: 2000-04-23
Also published as: EP1047435A1; EP1047435A4; AU1273399A

Abstract

This invention relates to a composition for use in the treatment of pathogenic microorganisms in a live animal, the composition comprising an electro-chemically activated, anion-containing aqueous solution.

Description

THE USE OF AN AQUEOUS SOLUTION IN THE PREPARATION OF A MEDICAMENT FOR USE IN THE TREATMENT OF LIVE ANIMALS

Field of the Invention :

This invention relates to the use of an aqueous solution in the preparation of

a medicament for use in the treatment of live animals.

Background to the Invention :

For the purposes of this specification, the term "animal" should be construed

to include within its meaning sheep, cattle, goats, pigs, chickens, ostriches,

reptiles and the like; the term "disease" should be construed to include within

its meaning diarrhoea; the term pathogen should be construed to include within

its meaning micro-organisms such as E-coli; and the term "medicament"

should be construed to include within its meaning oral bactericides and

bactericidal inhalants. The Applicant envisages that the invention will be

applicable particularly, but not exclusively, in the preparation of a medicament

for use in the treatment of pathogenic micro-organisms in weaner piglets and

chicklets.

The presence of antibiotic residues in food products lead to allergic and

anaphylactic reactions in humans. The development of resistant strains of

micro-organisms makes anti-microbials ineffective.

Object of the Invention ;

It is accordingly an object of the mvention to provide inexpensive, novel and

a^lternative anti-microbials that overcome the above disadvanta ges. In accordance with a first aspect of the invention, there is provided the use of

a composition in the preparation of a medicament for use in the treatment of

pathogenic micro-organisms in a live animal, the composition comprising an

electro-chemically activated anion-containing aqueous solution.

In accordance with a second aspect of the invention there is provided a

composition in the preparation of a medicament for the treatment of

pathogenic micro-organisms in live animals, the composition comprising an

electro-chemically activated anion-containing aqueous solution, the composition

substantially as herein defined.

In accordance with a third aspect of the invention there is provided a method

of treating pathogenic micro-organisms in a live animal, the method

including the step of administering a dosage of a composition comprising an

electro-chemically activated anion-containing aqueous solution to the animal,

the anion-containing aqueous solution being substantially as herein defined.

The anion-containing aqueous solution may be prepared by means of

electrolysis of an aqueous solution of a salt. The salt may be sodium chloride.

In particular, it may be non-iodated sodium chloride or potassium chloride. The anion-containing solution and the associated cation-containing solution

may be produced by an electro-chemical reactor or so-called electrolysis

device.

The electro-chemical reactor may include a through flow, electro-chemical cell

having two co-axial cylindrical electrodes with a co-axial diaphragm between

the electrodes so as to separate an annular inter electrode space into a catalytic

and an analytic chamber.

The anion-containing solution is referred to hereinafter for brevity as the

"anolyte solution" and the cation-containing solution is referred to hereinafter

for brevity as the "catholyte solution".

The anolyte solution may be produced from an aqueous NaCl solution,

electrolysed to produce radical cation and radical anion species, the anolyte

solution having a redox potential up to about + 600 mV to + 800 mV. These

species may be labile and after about 96 hours, the various radical species may

disappear with no residues being produced.

The anolyte solution may have a pH of about 6,5 to 7,5. The anolyte solution

may include species such as CIO; CIO^" ; HCIO; OH^" ; H0₂- ; H₂0₂ ; 0₃ ;

These species have been found to have a synergistic anti-bacterial and/or anti¬

viral effect which is generally stronger than that of chemical bactericides and

has been found to be particularly effective against viral organisms and spore

and cyst forming bacteria.

The redox potential of the anolyte solution may be monitored during the

process so that the treatment process may be monitored and controlled on a

continuous basis.

The catholyte solution generally may have a pH of up to about 12-13 and a

redox potential of about -980 mV. The catholyte solution may include species

such as NaOH; KOH; CA(OH)₂ ; Mg (OH)₂ ; HO^" ; H₃O₂ - ; HO₂- ; H₂0₂-;

O₂- ; OH^"; O₂ ²- .

The method of treatment may include administering the anolyte solution by

soaking, rinsing or dipping the animal in the anolyte solution, applying the

anolyte solution as an inhalant via an atomising or fogging process or

administering the anolyte solution orally. The soaking, rinsing or dipping

process is suitable for animals which can be handled with relative ease.

The processes of atomising or fogging and oral administration by addition to

drinking water are both suitable for animals such as weaner piglets and chicklets which are susceptible to stress and accompanying weight loss. The

atomising or fogging process may include the step of atomising the solution

into the atmosphere in a volume to be treated, forming droplets of between 5

and 100 micrometre. The method may include the preliminary step of

enclosing the volume to be treated prior to atomising or fogging the enclosed

volume.

The atomising or fogging process is preferably conducted at pre-determined

intervals so as to maintain a suitable level of anolyte concentration in the

atmosphere, thus utilising the optimum microcidal and other properties of the

anolyte solution in accordance with the required administration rate.

The anolyte solution may also be applied by an atomising process in air

ducting systems to destroy air-bome micro-organisms and to destroy micro¬

organisms present in the airways and lung tissue by inhalation.

The treatment of the animal as described above may be conducted so as to

improve the weight gain as a result of the anti-microbial action of the anolyte

solution.

The oxidising-free radicals present in the anolyte solution may act

synergistically at a bacterial cellular level. The anolyte solution may have a specific anion concentration and distribution

and a redox potential in accordance with the specific application.

The pathogenic micro-organisms to be treated may include enteric pathogenic

micro-organisms and respiratory pathogenic micro-organisms.

Detailed Description of the Invention :

A preferred embodiment of the invention will now be described with reference

to the accompanying experiments.

In a series of experiments, the bactericidal effect of the anolyte solution was

tested on animals. The results are reflected in the tables below.

An electro-chemical reactor, including a through flow, electro-chemical cell

having two co-axial cylindrical electrodes with a co-axial diaphragm between

them so as to separate an annular inter-electrode space into a catalytic and an

analytic chamber, was used to produce anolyte and catholyte solutions.

Experiment 1 - Weaner Piglets

The anolyte solution was added to the drinking water of the weaner piglets

over a period of 14 days and the results were measured in terms of average weight after the 14 day period. The average weight of the administered groups

were compared with the average weight of the unadministered groups.

The administered groups showed relative weight gain relative to the

unadministered groups. The relative weight gains of the administered groups

are reflected in Table 1 below.

Experiment 2 - Broilers (Chicklets)

Day old broilers were administered with anolyte solution (10% diluted) by

addition to drinking water for 7 days. (Group C3 - 12 000 chicklets). No

antibiotic medication was administered during that time. Untreated control

groups (Cl, C2, C4 and C5 = total 48 000 chicklets) received normal drinking

water during that time. The untreated groups were routinely medicated with

Tylosin for 3 consecutive days.

Bacterial analyses of the drinking water of all groups were regularly conducted

during the first 7 days. Other measurements included daily mortalities and

morbidities throughout and pH and ORP determinations of the drinking water

during the first 7 days. All results are reflected in Table 2 below.

Medication of drinking water with anolyte solution supplied to day-old chicklets for the first period resulted in a significant reduction in mortalities

throughout the growth and finishing period. Mortalities increased in all the

groups from the 4th week onwards mainly due to respiratory disease. It is

envisaged that these could be reduced by fogging the environment with anolyte

solution to eliminate airborne respiratory pathogens by means of respiratory

intake.

It has been found that the efficacy of the use of the anolyte solution in the

treatment of live animals depends upon the concentration of the anions in the

anolyte solution, as measured by the oxidation-reduction potential (ORP) or

redox potential of the anolyte solution, the flow rate through the reactor, the

exposure time, i.e. the contact time between the contaminated animal and the

anolyte solution and the temperature during application. By measuring the

redox potential of the anolyte solution during the treatment, for example, of

a weaner piglet, the available free radical concentration can be monitored.

Anolyte solution has been found to be more effective at lower than at higher

temperatures.

It will be appreciated that many variations in detail are possible without

departing from the scope and/or spirit of the invention as claimed in the claims

hereinafter.

Claims

1. The use of a composition in the preparation of a medicament for use in

the treatment of pathogenic micro-organisms in a live animal, the

composition comprising an electro-chemically activated, anion-

containing aqueous solution.

2. A composition for the preparation of a medicament for the treatment of

pathogenic micro-organisms in live animals, the composition comprising

an electro-chemically activated anion-containing aqueous solution.

3. A method of treating pathogenic micro-organisms in a live animal, the

method including the step of administering a dosage of a composition

comprising an electro-chemically activated anion-containing aqueous

solution to the animal

4. A composition as claimed in claim 2 wherein the anion-containing

aqueous solution is prepared by means of electrolysis of an aqueous

solution of a salt.

A composition as claimed in claim 2 wherein the anion-containing

solution is produced by an electro-chemical reactor, the electro-chemical reactor including a through flow, electro-chemical cell having two co¬

axial cylindrical electrodes with a co-axial diaphragm between the

electrodes so as to separate an annular inter electrode space into a

catalytic and an analytic chamber.

6. A composition as claimed in claim 2 wherein the anion containing

aqueous solution has a redox potential up to about ÷600 mV and 800

mV and a pH of about 6,5 to 7,5.

7. A method of treatment as claimed in claim 3 including at least one of

the steps of administering the solution by soaking, rinsing or dipping the

animal in the solution, applying the solution as an inhalant via an

atomising or fogging process, and administering the solution orally.

8. A method as claimed in claim 7 wherein the atomising or fogging

process includes the step of atomising the solution into the atmosphere

in a volume to be treated, forming droplets of between 5 and 100

micrometre..