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WO1999005266A3 - Trans-species nuclear transfer - Google Patents

Trans-species nuclear transfer Download PDF

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Publication number
WO1999005266A3
WO1999005266A3 PCT/US1998/015387 US9815387W WO9905266A3 WO 1999005266 A3 WO1999005266 A3 WO 1999005266A3 US 9815387 W US9815387 W US 9815387W WO 9905266 A3 WO9905266 A3 WO 9905266A3
Authority
WO
WIPO (PCT)
Prior art keywords
nuclear transfer
trans
donor cells
species nuclear
oocytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/015387
Other languages
French (fr)
Other versions
WO1999005266A2 (en
WO1999005266B1 (en
Inventor
Neal L First
Tanja Dominko
Maissam Mitalipoua
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Priority to AU85875/98A priority Critical patent/AU8587598A/en
Publication of WO1999005266A2 publication Critical patent/WO1999005266A2/en
Publication of WO1999005266A3 publication Critical patent/WO1999005266A3/en
Publication of WO1999005266B1 publication Critical patent/WO1999005266B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method of producing cloned nuclear transfer embryos from differentiated donor cells is described. The method includes culturing differentiated donor cells in low serum medium and using bovine oocytes as recipient oocytes for donor cells from different species. This method may be used to produce genetically identical animals and transgenic animals.
PCT/US1998/015387 1997-07-26 1998-07-24 Trans-species nuclear transfer Ceased WO1999005266A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85875/98A AU8587598A (en) 1997-07-26 1998-07-24 Trans-species nuclear transfer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5310397P 1997-07-26 1997-07-26
US60/053,103 1997-07-26

Publications (3)

Publication Number Publication Date
WO1999005266A2 WO1999005266A2 (en) 1999-02-04
WO1999005266A3 true WO1999005266A3 (en) 1999-04-15
WO1999005266B1 WO1999005266B1 (en) 1999-09-23

Family

ID=21981947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/015387 Ceased WO1999005266A2 (en) 1997-07-26 1998-07-24 Trans-species nuclear transfer

Country Status (2)

Country Link
AU (1) AU8587598A (en)
WO (1) WO1999005266A2 (en)

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WO1999046982A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd Porcine nuclear transfer
US7621606B2 (en) 2001-08-27 2009-11-24 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
JP3652308B2 (en) * 1999-06-30 2005-05-25 ソウル ナショナル ユニバーシティー インダストリー ファウンデーション Clone cattle production method
EP1109890A4 (en) * 1999-06-30 2004-12-29 Hwang Woo Suk METHOD FOR PRODUCING CLONED, HUMAN EMBRYOS BY MEANS OF INTERSPEZIES CORE TRANSPLANTING TECHNOLOGY.
WO2007019398A1 (en) 2005-08-03 2007-02-15 Advanced Cell Technology, Inc. Improved methods of reprogramming animal somatic cells
BR0012099A (en) * 1999-06-30 2003-07-29 Advanced Cell Tech Inc Cytoplasmic transfer to de-differentiate recipient cells
JP2003503045A (en) * 1999-06-30 2003-01-28 ワン，ウー−サク Clonal tiger production method using xenogeneic nuclear transfer technology
MXPA02002744A (en) * 1999-09-14 2003-07-21 Univ Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions.
CA2394614A1 (en) * 1999-12-20 2001-06-28 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Embryonic or stem-like cells produced by cross species nuclear transplantation
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
RS58255B1 (en) 2013-04-16 2019-03-29 Regeneron Pharma Targeted modification of rat genome
KR102170502B1 (en) 2013-12-11 2020-10-28 리제너론 파마슈티칼스 인코포레이티드 Methods and compositions for the targeted modification of a genome
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EP3221457B1 (en) 2014-11-21 2019-03-20 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modification using paired guide rnas
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JP7278978B2 (en) 2017-06-27 2023-05-22 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Non-human animals containing a humanized ASGR1 locus
EP3585159B1 (en) 2017-07-31 2025-05-14 Regeneron Pharmaceuticals, Inc. Method of testing the ability of a crispr/cas9 nuclease to modify a target genomic locus in vivo, by making use of a cas-transgenic mouse or rat which comprises a cas9 expression cassette in its genome
WO2019028029A1 (en) 2017-07-31 2019-02-07 Regeneron Pharmaceuticals, Inc. Assessment of crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo
CN111182790A (en) 2017-07-31 2020-05-19 瑞泽恩制药公司 CRISPR reporter non-human animals and uses thereof
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TW202112229A (en) 2019-06-07 2021-04-01 美商雷傑納榮製藥公司 Non-human animals comprising a humanized albumin locus
CN114008193A (en) 2019-06-27 2022-02-01 瑞泽恩制药公司 Modeling of TDP-43 proteinopathy
WO2021108363A1 (en) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Crispr/cas-mediated upregulation of humanized ttr allele
EP4096396A1 (en) 2020-01-28 2022-12-07 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized pnpla3 locus and methods of use
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US20250017183A1 (en) 2021-11-04 2025-01-16 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a modified cacng1 locus
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US20250002946A1 (en) 2023-06-30 2025-01-02 Regeneron Pharmaceuticals, Inc. Methods And Compositions For Increasing Homology-Directed Repair
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Citations (4)

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US5496720A (en) * 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer
WO1998007841A1 (en) * 1996-08-19 1998-02-26 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation
WO1998030683A2 (en) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Nuclear transfer with differentiated fetal and adult donor cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496720A (en) * 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer
WO1998007841A1 (en) * 1996-08-19 1998-02-26 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation
WO1998030683A2 (en) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Nuclear transfer with differentiated fetal and adult donor cells

Non-Patent Citations (6)

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Title
CHESNE P ET AL: "NUCLEAR TRANSFER IN CATTLE: BIRTH OF CLONED CALVES AND ESTIMATION OF BLASTOMERE TOTIPOTENCY IN MORULAE USED AS A SOURCE OF NUCLEI", LIFE SCIENCES, vol. 316, 1993, pages 487 - 491, XP000197855 *
HEYMAN Y ET AL: "CLONING OF DOMESTIC SPECIES", ANIMAL REPRODUCTION SCIENCE, vol. 42, no. 1/04, 1996, pages 427 - 436, XP000671884 *
SIMS M ET FIRST: "PRODUCTION OF CALVES BY TRANSFER OF NUCLEI FROM CULTURED INNER CELL MASS CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 90, no. 13, June 1993 (1993-06-01), pages 6143 - 6147, XP000604993 *
STICE S L ET AL: "PLURIPOTENT BOVINE EMBRYONIC CELL LINES DIRECT EMBRYONIC DEVELOPMENT FOLLOWING NUCLEAR TRANSFER", BIOLOGY OF REPRODUCTION, vol. 54, no. 1, January 1996 (1996-01-01), pages 100 - 110, XP000671718 *
WOLFE, B.A. ET AL.: "Embryos produced by the transfer of caprine nuclei to enucleated bovine oocytes are capable of cleavage but not to develop to blastocysts", BIOLOGY OF REPRODUCTION, vol. 50, no. suppl. 1, 1994 - 1972, pages 72, XP002092259 *
ZAWADA, W.M. ET AL.: "Somatic cell cloned transgenic bovine neurons for transplantation in Parkinsonian rats", NATURE MEDICINE, vol. 4, no. 5, May 1998 (1998-05-01), pages 569 - 574, XP002092260 *

Also Published As

Publication number Publication date
AU8587598A (en) 1999-02-16
WO1999005266A2 (en) 1999-02-04
WO1999005266B1 (en) 1999-09-23

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