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WO1999005265A3 - Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance - Google Patents

Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance Download PDF

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Publication number
WO1999005265A3
WO1999005265A3 PCT/US1998/015289 US9815289W WO9905265A3 WO 1999005265 A3 WO1999005265 A3 WO 1999005265A3 US 9815289 W US9815289 W US 9815289W WO 9905265 A3 WO9905265 A3 WO 9905265A3
Authority
WO
WIPO (PCT)
Prior art keywords
herbicide resistance
plants
production
plastid transformation
transgenic plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/015289
Other languages
French (fr)
Other versions
WO1999005265A2 (en
Inventor
Alan D Blowers
John Sanford
Ana Maria Bailey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional
Sanford Scientific Inc
Original Assignee
Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional
Sanford Scientific Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Sanford Scientific Inc filed Critical Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional
Priority to AU85837/98A priority Critical patent/AU8583798A/en
Publication of WO1999005265A2 publication Critical patent/WO1999005265A2/en
Publication of WO1999005265A3 publication Critical patent/WO1999005265A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a method of producing a herbicide-resistant plant, which method entails delivering one or more herbicide resistance-conferring selectable marker genes into and expressing the same within the plastid of the plant, both in photosynthetic as well as non-photosynthetic cells. Nucleic acids for transformation and multicellular plants whose plastids have been transformed are also provided.
PCT/US1998/015289 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance Ceased WO1999005265A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85837/98A AU8583798A (en) 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of trans genic plants with herbicide resistance

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US89906197A 1997-07-23 1997-07-23
US08/899,061 1997-07-23

Publications (2)

Publication Number Publication Date
WO1999005265A2 WO1999005265A2 (en) 1999-02-04
WO1999005265A3 true WO1999005265A3 (en) 1999-05-20

Family

ID=25410433

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/015289 Ceased WO1999005265A2 (en) 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance

Country Status (4)

Country Link
AR (1) AR015136A1 (en)
AU (1) AU8583798A (en)
WO (1) WO1999005265A2 (en)
ZA (1) ZA986574B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6858777B2 (en) 2000-08-11 2005-02-22 Syngenta Participations Ag Methods for stable transformation of plants
US7645918B2 (en) 2002-08-08 2010-01-12 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Method of plastid transformation in asteraceae, vector for use therein and plants thus obtained
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
ES2281183T3 (en) * 1998-07-10 2007-09-16 Calgene Llc EXPRESSION OF GENES OF TOLERANCE TO HERBICIDE IN VEGETABLE PLASTIDES.
US6492578B1 (en) * 1998-07-10 2002-12-10 Calgene Llc Expression of herbicide tolerance genes in plant plastids
BR9912745A (en) 1998-08-04 2001-11-06 Cargill Inc Promoters of plant fatty acid denaturase
US6515206B1 (en) 1998-12-23 2003-02-04 Calgene Llc Plastid transformation of Brassica
AR024934A1 (en) * 1999-07-27 2002-10-30 Novartis Ag CHEMICAL GENES
SI1240340T1 (en) 1999-12-16 2012-09-28 Monsanto Technology Llc Dna constructs for expression of heterologous polypeptides in plants
US20030041353A1 (en) * 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
EP1263281B1 (en) * 2000-02-29 2012-04-11 Auburn University Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
CN100480376C (en) 2000-03-22 2009-04-22 依康遗传学股份有限公司 Method for transforming plant plastides and making transplastomic plants
WO2001081600A2 (en) * 2000-04-20 2001-11-01 Btg International Limited Transgenic plants
US6781033B2 (en) 2000-04-26 2004-08-24 Monsanto Technology Llc Method for the transformation of plant cell plastids
AU2012200277B2 (en) * 2002-08-08 2014-11-06 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Method of plastid transformation in Asteraceae, vector for use therein and plants thus obtained
EP2046959B1 (en) 2006-07-12 2015-11-18 Commonwealth Scientific and Industrial Research Organisation Wheat and barley with enhanced salinity tolerance
NZ577363A (en) * 2006-12-07 2012-02-24 Dow Agrosciences Llc Transgenic plants cells comprising the phosphinothricin herbicide resistant gene DSM-2 from Streptomyces coelicolor A3
CN101977510A (en) 2007-06-29 2011-02-16 联邦科学技术研究组织 Methods for degrading toxic compounds
EP2190970B1 (en) 2007-08-13 2024-11-06 Commonwealth Scientific and Industrial Research Organisation Barley with low levels of hordein
WO2009129583A1 (en) 2008-04-25 2009-10-29 Commonwealth Scientific Industrial Research Organisation Recombinant cells and methods for hydroxylating fatty acids
UA106729C2 (en) 2008-08-25 2014-10-10 Коммонвелт Сайєнтіфік Енд Індастріал Рісерч Організейшн Transgenic plants having enhanced resistance to plants pathogens
ES2644883T3 (en) 2008-11-18 2017-11-30 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods to produce omega-3 fatty acids
AR083323A1 (en) 2010-06-28 2013-02-21 Commw Scient Ind Res Org METHODS TO PRODUCE LIPIDS
EP2798063B1 (en) 2011-12-27 2022-03-30 Commonwealth Scientific and Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
AU2012324003B2 (en) 2011-12-27 2016-05-26 Commonwealth Scientific And Industrial Research Organisation Simultaneous gene silencing and supressing gene silencing in the same cell
AR089442A1 (en) 2011-12-27 2014-08-20 Commw Scient Ind Res Org PROCESSES TO PRODUCE LIPIDS
AU2014280852B2 (en) 2013-06-13 2017-11-09 Commonwealth Scientific And Industrial Research Organisation Barley with very low levels of hordeins
DK3036328T3 (en) 2013-08-21 2022-01-24 Commw Scient Ind Res Org GEN FOR RUST RESISTANCE
US9725399B2 (en) 2013-12-18 2017-08-08 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
SG11201610596PA (en) 2014-06-27 2017-01-27 Commw Scient Ind Res Org Lipid comprising docosapentaenoic acid
WO2016004473A1 (en) 2014-07-07 2016-01-14 Commonwealth Scientific And Industrial Research Organisation Processes for producing industrial products from plant lipids
KR20180109859A (en) 2015-11-18 2018-10-08 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 Rice grains with thickened hulls
US10941428B2 (en) 2018-09-12 2021-03-09 Universidad Politécnica de Madrid Reagents and methods for the expression of an active NifB protein and uses thereof
AU2020236396A1 (en) 2019-03-08 2021-10-14 Commonwealth Scientific And Industrial Research Organisation Expression of nitrogenase polypeptides in plant cells

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0223247A2 (en) * 1985-11-22 1987-05-27 Ciba-Geigy Ag Direct gene transfer into plastids and mitochondria
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
US5217902A (en) * 1989-05-26 1993-06-08 Dna Plant Technology Corporation Method of introducing spectinomycin resistance into plants
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
WO1997032977A1 (en) * 1996-03-06 1997-09-12 Rutgers University Plastid transformation in arabidopsis thaliana
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
WO1998002562A2 (en) * 1996-07-16 1998-01-22 Rhone-Poulenc Agrochimie Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0223247A2 (en) * 1985-11-22 1987-05-27 Ciba-Geigy Ag Direct gene transfer into plastids and mitochondria
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5217902A (en) * 1989-05-26 1993-06-08 Dna Plant Technology Corporation Method of introducing spectinomycin resistance into plants
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
WO1997032977A1 (en) * 1996-03-06 1997-09-12 Rutgers University Plastid transformation in arabidopsis thaliana
WO1998002562A2 (en) * 1996-07-16 1998-01-22 Rhone-Poulenc Agrochimie Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides

Non-Patent Citations (7)

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Title
A. PEÑAZOLA-VAZQUEZ ET AL.,: "Cloning and sequencing of the genes involved in glyphosate utilization in Pseudomonas pseudomallei", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 2, 1995, pages 538 - 543, XP002096052 *
A. PEÑAZOLA-VAZQUEZ ET AL.,: "Expresson of hygromycin B phosphotransferase gene confers tolerance to the herbicide glyphosate", PLANT CELL REPORTS, vol. 14, 1995, pages 482 - 487, XP002096051 *
DANIELL ET AL: "containment of herbicide resistance through genetic engineering of the chloroplast genome", NATURE BIOTECHNOLOGY, vol. 16, April 1998 (1998-04-01), pages 345 - 348, XP002090409 *
D'HALLUIN K ET AL: "TRANSFORMATION OF SUGARBEET (BETA VULGARIS L.) AND EVALUATION OF HERBICIDE RESISTANCE IN TRANSGENIC PLANTS", BIO/TECHNOLOGY, vol. 10, March 1992 (1992-03-01), pages 309 - 314, XP002056296 *
RATHORE K S ET AL: "USE OF BAR AS A SELECTABLE MARKER GENE AND FOR THE PRODUCTION OF HERBICIDE-RESISTANT RICE PLANTS FROM PROTOPLASTS", PLANT MOLECULAR BIOLOGY, vol. 21, no. 5, March 1993 (1993-03-01), pages 871 - 884, XP002064546 *
WOHLLEBEN W ET AL: "NUCLEOTIDE SEQUENCE OF THE PHOSPHINOTHRICIN N-ACETYLTRANSFERASE GENE FROM STREPTOMYCES VIRIDOCHROMOGENES TUE494 AND ITS EXPRESSION IN NICOTIANA TABACUM", GENE, vol. 70, 1988, pages 25 - 37, XP002071460 *
ZORA SVAB ET AL: "STABLE TRANSFORMATION OF PLASTIDS IN HIGHER PLANTS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 87, no. 21, 1 November 1990 (1990-11-01), pages 8526 - 8530, XP000164753 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6858777B2 (en) 2000-08-11 2005-02-22 Syngenta Participations Ag Methods for stable transformation of plants
US7645918B2 (en) 2002-08-08 2010-01-12 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Method of plastid transformation in asteraceae, vector for use therein and plants thus obtained
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form

Also Published As

Publication number Publication date
ZA986574B (en) 2000-01-12
WO1999005265A2 (en) 1999-02-04
AU8583798A (en) 1999-02-16
AR015136A1 (en) 2001-04-18

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