[go: up one dir, main page]

WO1999001570A3 - Methods for accelerating hybridization of nucleic acid molecules - Google Patents

Methods for accelerating hybridization of nucleic acid molecules Download PDF

Info

Publication number
WO1999001570A3
WO1999001570A3 PCT/CA1998/000631 CA9800631W WO9901570A3 WO 1999001570 A3 WO1999001570 A3 WO 1999001570A3 CA 9800631 W CA9800631 W CA 9800631W WO 9901570 A3 WO9901570 A3 WO 9901570A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
methods
acid molecules
hybridization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA1998/000631
Other languages
French (fr)
Other versions
WO1999001570A2 (en
Inventor
Robert N Bryan
Lynn P Cloney
Barbara A Farnworth
A Johanna Marostenmaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ID Biomedical Corp of Quebec
Original Assignee
ID Biomedical Corp of Quebec
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ID Biomedical Corp of Quebec filed Critical ID Biomedical Corp of Quebec
Priority to AU83272/98A priority Critical patent/AU8327298A/en
Publication of WO1999001570A2 publication Critical patent/WO1999001570A2/en
Publication of WO1999001570A3 publication Critical patent/WO1999001570A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of increasing the hybridization rate of a first nucleic acid molecule to a second nucleic acid molecule is provided, comprising the step of mixing a first nucleic acid molecule, a second nucleic acid molecule, and polyamine and/or RNase H under conditions and for a time sufficient to permit hybridization of said first nucleic acid molecule to the second nucleic acid molecule.
PCT/CA1998/000631 1997-07-03 1998-07-03 Methods for accelerating hybridization of nucleic acid molecules Ceased WO1999001570A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU83272/98A AU8327298A (en) 1997-07-03 1998-07-03 Methods for accelerating hybridization of nucleic acid molecules

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US5182797P 1997-07-03 1997-07-03
US60/051,827 1997-07-03
US8602198P 1998-05-18 1998-05-18
US60/086,021 1998-05-18
US9027398P 1998-06-22 1998-06-22
US60/090,273 1998-06-22

Publications (2)

Publication Number Publication Date
WO1999001570A2 WO1999001570A2 (en) 1999-01-14
WO1999001570A3 true WO1999001570A3 (en) 1999-08-05

Family

ID=27368023

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1998/000631 Ceased WO1999001570A2 (en) 1997-07-03 1998-07-03 Methods for accelerating hybridization of nucleic acid molecules

Country Status (2)

Country Link
AU (1) AU8327298A (en)
WO (1) WO1999001570A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003064679A2 (en) 2002-01-30 2003-08-07 Id Biomedical Corporation Methods for detecting vancomycin-resistant microorganisms and compositions therefor
US10876149B2 (en) 2014-10-22 2020-12-29 Prominex, Inc. Compositions and methods for the detection of nucleic acids
WO2017189878A1 (en) * 2016-04-27 2017-11-02 Roka Bioscience, Inc. Compositions and methods for the detection of nucleic acids

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229442A1 (en) * 1986-01-07 1987-07-22 Gen-Probe Incorporated Accelerated nucleic acid reassociation method
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993001311A1 (en) * 1991-07-01 1993-01-21 Amersham International Plc Enhancement of polynucleotide hybridization
WO1993015226A1 (en) * 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
WO1997043450A1 (en) * 1996-05-16 1997-11-20 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229442A1 (en) * 1986-01-07 1987-07-22 Gen-Probe Incorporated Accelerated nucleic acid reassociation method
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993001311A1 (en) * 1991-07-01 1993-01-21 Amersham International Plc Enhancement of polynucleotide hybridization
WO1993015226A1 (en) * 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
WO1997043450A1 (en) * 1996-05-16 1997-11-20 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays

Also Published As

Publication number Publication date
AU8327298A (en) 1999-01-25
WO1999001570A2 (en) 1999-01-14

Similar Documents

Publication Publication Date Title
CA2153898A1 (en) Method for generating single-stranded dna molecules
CA2205382A1 (en) Enzymatic dna molecules
WO1997026270A3 (en) Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules
WO1993019202A3 (en) Exchangeable template reaction
DE69636080D1 (en) A cascading amplification reaction of nucleic acids
WO1998005673A8 (en) Methods for purifying nucleic acids
EP1408122A3 (en) Methods and compositions for efficient nucleic acid sequencing
WO1997027325A3 (en) Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
WO1999010358A3 (en) Method for producing nucleic acid polymers
EP2236622A3 (en) Method for generating circularised nucleic acid
WO1999055857A3 (en) Nucleoside triphosphates and their incorporation into ribozymes
AU2500197A (en) Method, apparatus and kits for sequencing of nucleic acids using multiple dyes
AU9125198A (en) Compositions, kits, and methods for effecting adenine nucleotide modulation of dna mismatch recognition proteins
CA2273078A1 (en) Use of nucleic acids bound to carrier macromolecules
AU3595493A (en) Method for nucleic acid hybridization using single-stranded DNA binding protein
WO1998035060A8 (en) Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO2000018914A3 (en) Novel dkr polypeptides
WO1996018744A3 (en) Dna purification by forming a triple helix with an immobilized oligonucleotide
WO1998028317A3 (en) Synthesis of nucleosides and polynucleotides
CA2239005A1 (en) Production of ammonium acrylate
WO1995029236A3 (en) Lymphocyte activation antigens and antibodies thereto
EP0725137A4 (en) KETO GROUP INTRODUCTIVE ENZYME, ENCODING DNA, AND METHOD FOR PRODUCING KETOCAROTENOID
EP0261955A3 (en) Process for immobilization of dna
AU1062997A (en) Novel polymer supports for nucleic acid synthesis
WO2002083893A3 (en) Isolation of dna molecules using mercapto-aryl ligands

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM GW HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM GW HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

NENP Non-entry into the national phase

Ref country code: KR

NENP Non-entry into the national phase

Ref country code: JP

Ref document number: 1999506007

Format of ref document f/p: F

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

122 Ep: pct application non-entry in european phase