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WO1999001034A1 - Procede de repousse des cheveux - Google Patents

Procede de repousse des cheveux Download PDF

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Publication number
WO1999001034A1
WO1999001034A1 PCT/US1998/013754 US9813754W WO9901034A1 WO 1999001034 A1 WO1999001034 A1 WO 1999001034A1 US 9813754 W US9813754 W US 9813754W WO 9901034 A1 WO9901034 A1 WO 9901034A1
Authority
WO
WIPO (PCT)
Prior art keywords
human
dermal papilla
papilla cells
cells
follicle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/013754
Other languages
English (en)
Inventor
Jerry E. Cooley
James E. Vogel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU82829/98A priority Critical patent/AU8282998A/en
Publication of WO1999001034A1 publication Critical patent/WO1999001034A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes

Definitions

  • the present invention is directed to a method for producing new hair growth in humans by implanting human dermal papilla cells into human scalp skin, wherein the human dermal papilla cells are cultured in a medium supplemented with a conditioned medium.
  • the conditioned medium is formed from a culture of normal human keratinocytes.
  • Hair loss is a very common human condition and many people desire treatment or correction for this problem.
  • the leading cause for hair loss is the influence of androgens on genetically susceptible follicles (i.e. "androgenetic alopecia").
  • Numerous treatments are available but all have significant drawbacks.
  • Pharmacologic treatments attempt to revive and stimulate existing hair follicles. Because androgenetic alopecia may lead to the complete disappearance of hair follicles, pharmacologic treatments may always be of limited value.
  • Hair restoration surgery involves the redistribution of permanent hair follicles to hairless areas but because the amount of hair follicles available for redistribution is limited, the ability of this teaching and to completely correct baldness is also limited.
  • the purpose of the present invention is to overcome the limitations of the known treatments.
  • a method of creating new follicles from existing follicles is needed, thus providing a virtually unlimited supply of hair for treating baldness.
  • Previous research in rats has shown that hair follicles can actually be regenerated when the critical cellular component of the follicle, the papilla, is expanded using cell culture and transplanted into the skin (Jahoda CAB, Reynolds AJ, Oliver RF. Induction Of Hair Growth In Ear Wounds By Cultured Dermal Papilla Cells. J Invest Dermatol 101:584-590, 1993).
  • One major limitation of this technique is that when the papilla cells are expanded in culture through serial passage, they lose their ability to regenerate hair.
  • An object of the present invention is to provide a method of inducing hair growth in humans by implanting human dermal papilla cells into human skin.
  • the method comprises the steps of removing a papilla from a human hair follicle, isolating the dermal papilla cells from the follicle to form a cell suspension, culturing such cells in a medium supplemented with a conditioned medium taken from human keratinocyte culture, and implanting the papilla cells so cultured into the epidermis layer of the skin in contact with an epidermal cell.
  • Another object of the present invention is to provide a method for growing human dermal papilla cells in a medium supplemented with a conditioned medium that is formed by culturing human keratinocytes, taken from the epidermis of the follicle, for example, in the keratinocyte culture medium.
  • the papilla cells so cultured can expand rapidly for many passages in vitro while maintaining their hair inducing properties.
  • the implantation technique of the present invention is initiated by isolating and removing a papilla from the base of hair follicles from an area of a patient's scalp where hair loss is not expected to occur (e.g. the "donor area" used in hair transplantation comprising the mid temporo-parieto-occipital region of scalp).
  • a papilla sample are obtained by a punch biopsy or an excisional technique similar to that used in hair transplantation in which tumescent anesthesia is used and the tissue is removed with strip or elliptical harvesting.
  • the papilla is dissected free from the remaining part of the follicle, which is attached to and taken out together with the papilla, under microscopy.
  • the dissected papilla is then initiated into a culture medium, either by simply floating the papilla in a medium containing a buffered salt solution with bovine serum to produce a cell suspension, or preferably, using a technique known to enhance the initiation of the papilla into culture (Warren R, Chestnut MH, Wong TK, et al. Improved Method For The Isolation And Cultivation Of Human Scalp Dermal Papilla Cells. J Invest
  • the papilla cells are cultured in M199 or similar buffered salt solution with fetal calf serum in a concentration of 10 to 20% or in Chang's media, either of which may be supplemented with known papilla growth factors such as fibroblast, vascular endothelial, or platelet derived growth factors, and/or other known papilla stimulators.
  • these media e.g. M199 with 10% fetal calf serum or Chang's media
  • a conditioned medium formed from the culmres of normal human keratinocytes which are taken from the epidermis, or preferably from the outer root sheath of follicles, as described by Takashi M. et al.
  • the keratinocytes are taken from the middle to lower portion of the follicles where the epithelial stem cells are thought to reside.
  • the keratinocytes may be cultured in standard fashion but may also be treated with epithelial mitogens such as minoxidil or insulin-like growth factor.
  • the source of these keratinocytes is from the same patient being treated (autologous source) but it may also be allogenic, such as from neonatal foreskin.
  • the cells may be passed in a routine fashion.
  • the use of keratinocyte conditioned media allows for the rapid expansion of papilla cells while maintaining their hair inducing properties. Therefore cells of low or high passage may be used, the latter being used to maximize cell number from a single papilla.
  • the media containing animal sera is removed and the cells are incubated with or without the patient's autologous sera mixed in M199 or a similar salt solution.
  • the papilla cells are harvested from the culture dish either by physically removing them or by enzymatic digestion such as with trypsin.
  • the cells are either used directly or are subsequently aggregated such as with centrifugation.
  • the cells may then be mixed with substances which can promote aggregation as well as easy introduction into the skin, such as fibronectin, glycosaminoglycans (e.g. dermatin sulphate, chondroitan sulfate, proteoglycans, heparan sulphate), collagen, and/or other substances known to fulfill this function.
  • substances which can promote aggregation as well as easy introduction into the skin such as fibronectin, glycosaminoglycans (e.g. dermatin sulphate, chondroitan sulfate, proteoglycans, heparan sulphate), collagen, and/or other substances known to fulfill this function.
  • the recipient site of the patient who is subject to the hair implantation may be pretreated with physical and/or pharmacologic methods to increase the receptiveness to the introduced cellular material.
  • the physical methods include ultraviolet light, ultrasound, massage, or the like.
  • Pharmacologic methods include topical minoxidil solution, or tretinoin solution or oral minoxidil, vitamins, or antioxidants, etc.
  • the harvested papilla cells may then be introduced into the area of the skin subject to the implantation using various techniques. For example, the papilla cells may be introduced into a blister on the skin such as that formed by a suction device; or the cells may be introduced into slit incisions in the skin made with a scalpel blade or hypodermic needle.
  • the cells may be introduced into the superficial layer of the skin by injection with a syringe and hypodermic needle.
  • the amount of papilla cells for the implantation for each opening of the skin is preferably about 100,000, but may range from 1,000 to 1,000,000, depending on the size and depth of the opening and the overall viability and activity of the cells.
  • the amount of the cells introduced may also be varied to vary the size of the subsequently produced hair.
  • the papilla cells introduced into the skin opening by the above described implantation techniques are in physical contact with the native epidermal cells.
  • the papilla cells may also be implanted by a co- introduction of a rigid shaft to promote the correct downward migration of the cells and proper orientation of the future follicle.
  • an absorbable suture material such as poly gly colic, polyester, or polydioxanone, preferably coated with a substance known to promote cellular adhesion and migration, such as fibronectin, collagen, and/or glycosaminoglycans.
  • the dermal papilla cells may be implanted together with epidermal cells, which are either interfollicular epidermal cells or those isolated from the outer root sheath of the follicle.
  • epidermal cells are from the same patient being treated.
  • the patient may be treated with either topical or systemic agents known to promote hair growth, including minoxidil, tretinoin, cyclosporine, finasteride, etc. Hair growth is expected over the subsequent weeks.
  • dermal papilla cells at any stage are cryopreserved and therefore may be thawed and used at any time in the future.

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Dermatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé qui permet la repousse des cheveux chez l'humain et qui consiste à implanter des cellules de papille dermique humaine dans le cuir chevelu de l'humain, lesdites cellules de papille dermique humaine étant cultivées dans un milieu complété d'un milieu conditionné. Le milieu conditionné est formé à partir d'une culture de kératinocytes humains normaux.
PCT/US1998/013754 1997-07-01 1998-07-01 Procede de repousse des cheveux Ceased WO1999001034A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU82829/98A AU8282998A (en) 1997-07-01 1998-07-01 Method for producing new hair growth

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5145997P 1997-07-01 1997-07-01
US60/051,459 1997-07-01

Publications (1)

Publication Number Publication Date
WO1999001034A1 true WO1999001034A1 (fr) 1999-01-14

Family

ID=21971437

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/013754 Ceased WO1999001034A1 (fr) 1997-07-01 1998-07-01 Procede de repousse des cheveux

Country Status (2)

Country Link
AU (1) AU8282998A (fr)
WO (1) WO1999001034A1 (fr)

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001058413A1 (fr) * 2000-02-08 2001-08-16 Klaus Rennebeck Procede pour stimuler la pousse des cheveux et kit pour mettre ledit procede en oeuvre
WO2002060396A3 (fr) * 2001-01-29 2003-03-13 Bioamide Inc Neo genese de follicules capillaires par injection de cellules progenitrices de follicules
WO2003051419A1 (fr) * 2001-12-19 2003-06-26 Henkel Kommanditgesellschaft Auf Aktien Equivalent dermique/capillaire avec papille reconstruite
WO2003088935A1 (fr) * 2002-04-17 2003-10-30 Aderans Research Institute, Inc. Compositions et procedes pour induire la formation de nouveaux follicules pileux et la croissance capillaire en une orientation voulue
US6817495B1 (en) 1999-10-05 2004-11-16 Mi-Ok Pty Ltd. Portable tool box
WO2005018731A1 (fr) * 2003-08-26 2005-03-03 Alza Corporation Dispositif et procede pour l'implantation cellulaire intradermique
WO2005053763A1 (fr) 2003-12-05 2005-06-16 Biointegrence Inc. Procede de pousse de cheveux
WO2007047707A1 (fr) * 2005-10-17 2007-04-26 Aderans Research Institute, Inc. Procédé consistant à insérer des cellules dans la peau
WO2005084223A3 (fr) * 2004-02-27 2007-05-31 Gen Hospital Corp Procedes et compositions pour la croissance capillaire
US7354980B1 (en) 2004-03-12 2008-04-08 Key Medical Technologies, Inc. High refractive index polymers for ophthalmic applications
US7446157B2 (en) 2004-12-07 2008-11-04 Key Medical Technologies, Inc. Nanohybrid polymers for ophthalmic applications
US7597885B2 (en) 2004-03-26 2009-10-06 Aderans Research Institute, Inc. Tissue engineered biomimetic hair follicle graft
US7780635B2 (en) 2006-02-09 2010-08-24 Aderans Research Institute, Inc. Apparatus and methods for delivering fluid and material to a subject
US7785876B2 (en) * 2002-11-14 2010-08-31 Aderans Research Institute, Inc. Cultivation of hair inductive cells
EP1874265A4 (fr) * 2005-03-29 2012-09-26 Univ Pennsylvania Procedes pour la generation de nouveaux follicules capillaires, de traitement de la calvitie, et l'elimination de poils
AU2012204059B2 (en) * 2005-03-29 2015-01-15 The Trustees Of The University Of Pennsylvania Methods for generating new hair follicles, treating baldness and hair removal
US8980628B2 (en) 2006-03-17 2015-03-17 Aderans Research Institute, Inc. Hair follicle precursor production by co-culturing mammalian dermal papilla cells and keratinocytes
US9023380B2 (en) 2005-11-22 2015-05-05 Aderans Research Institute, Inc. Hair follicle graft from tissue engineered skin
US11207511B2 (en) 2010-12-06 2021-12-28 Follica, Inc. Methods for treating baldness and promoting hair growth

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4919664A (en) * 1986-02-21 1990-04-24 Oliver Roy F Stimulation of hair growth
US5130142A (en) * 1990-10-31 1992-07-14 The Practer & Gamble Company Hair growth regulating composition comprising epithelium cell supernatant-derived growth factor
EP0682107A2 (fr) * 1994-04-11 1995-11-15 Research Development Corporation Of Japan Procédé pour la culture à long terme de cellules de la papille dermale

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4919664A (en) * 1986-02-21 1990-04-24 Oliver Roy F Stimulation of hair growth
US5130142A (en) * 1990-10-31 1992-07-14 The Practer & Gamble Company Hair growth regulating composition comprising epithelium cell supernatant-derived growth factor
EP0682107A2 (fr) * 1994-04-11 1995-11-15 Research Development Corporation Of Japan Procédé pour la culture à long terme de cellules de la papille dermale

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JAHODA C. A. B., REYNOLDS A. J.: "DERMAL-EPIDERMAL INTERACTIONS. ADULT FOLLICLE-DERIVED CELL POPULATIONS AND HAIR GROWTH.", DERMATOLOGIC CLINICS., W.B. SAUNDERS CO., LONDON., GB, vol. 14., no. 04., 1 October 1996 (1996-10-01), GB, pages 573 - 583., XP002913549, ISSN: 0733-8635, DOI: 10.1016/S0733-8635(05)70385-5 *
TAKASHI MATSUZAKI, MUTSUMI INAMATSU, KATSUTOSHI YOSHIZATO: "THE UPPER DERMAL SHEATH HAS A POTENTIAL TO REGENERATE THE HAIR IN THE RAT FOLLICULAR EPIDERMIS", DIFFERENTIATION., SPRINGER VERLAG., DE, vol. 60, no. 05, 1 September 1996 (1996-09-01), DE, pages 287 - 297, XP002913548, ISSN: 0301-4681, DOI: 10.1046/j.1432-0436.1996.6050287.x *
WARREN R, WONG T K: "STIMULATION OF HUMAN SCALP PAPILLA CELLS BY EPITHELIAL CELLS", ARCHIVES OF DERMATOLOGICAL RESEARCH., SPRINGER, INTERNATIONAL, BERLIN., DE, vol. 286, no. 01, 1 January 1994 (1994-01-01), DE, pages 01 - 05, XP002913547, ISSN: 0340-3696, DOI: 10.1007/BF00375835 *

Cited By (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6817495B1 (en) 1999-10-05 2004-11-16 Mi-Ok Pty Ltd. Portable tool box
WO2001058413A1 (fr) * 2000-02-08 2001-08-16 Klaus Rennebeck Procede pour stimuler la pousse des cheveux et kit pour mettre ledit procede en oeuvre
WO2002060396A3 (fr) * 2001-01-29 2003-03-13 Bioamide Inc Neo genese de follicules capillaires par injection de cellules progenitrices de follicules
WO2003051419A1 (fr) * 2001-12-19 2003-06-26 Henkel Kommanditgesellschaft Auf Aktien Equivalent dermique/capillaire avec papille reconstruite
WO2003088935A1 (fr) * 2002-04-17 2003-10-30 Aderans Research Institute, Inc. Compositions et procedes pour induire la formation de nouveaux follicules pileux et la croissance capillaire en une orientation voulue
US7785876B2 (en) * 2002-11-14 2010-08-31 Aderans Research Institute, Inc. Cultivation of hair inductive cells
WO2005018731A1 (fr) * 2003-08-26 2005-03-03 Alza Corporation Dispositif et procede pour l'implantation cellulaire intradermique
JP2007503876A (ja) * 2003-08-26 2007-03-01 アルザ・コーポレーシヨン 皮内細胞移植のためのデバイスおよび方法
EP1702632A4 (fr) * 2003-12-05 2009-11-11 Biointegrence Inc Procede de pousse de cheveux
WO2005053763A1 (fr) 2003-12-05 2005-06-16 Biointegrence Inc. Procede de pousse de cheveux
US7981629B2 (en) 2004-02-27 2011-07-19 The General Hospital Corporation Methods of isolating dermal papilla cells
WO2005084223A3 (fr) * 2004-02-27 2007-05-31 Gen Hospital Corp Procedes et compositions pour la croissance capillaire
US7476512B2 (en) 2004-02-27 2009-01-13 The General Hospital Corporation Methods of identifying dermal papilla cells
US7354980B1 (en) 2004-03-12 2008-04-08 Key Medical Technologies, Inc. High refractive index polymers for ophthalmic applications
US7597885B2 (en) 2004-03-26 2009-10-06 Aderans Research Institute, Inc. Tissue engineered biomimetic hair follicle graft
US7745555B2 (en) 2004-12-07 2010-06-29 Key Medical Technologies, Inc. Nanohybrid polymers for ophthalmic applications
US10421830B2 (en) 2004-12-07 2019-09-24 Key Medical Technologies, Inc. Nanohybrid polymers for ophthalmic applications
US7446157B2 (en) 2004-12-07 2008-11-04 Key Medical Technologies, Inc. Nanohybrid polymers for ophthalmic applications
US9056934B2 (en) 2004-12-07 2015-06-16 Key Medical Technologies, Inc. Nanohybrid polymers for ophthalmic applications
US9700502B2 (en) 2005-03-29 2017-07-11 The Trustees Of The University Of Pennsylvania Methods for generating new hair follicles, treating baldness, and hair removal
EP1874265A4 (fr) * 2005-03-29 2012-09-26 Univ Pennsylvania Procedes pour la generation de nouveaux follicules capillaires, de traitement de la calvitie, et l'elimination de poils
AU2012204059B2 (en) * 2005-03-29 2015-01-15 The Trustees Of The University Of Pennsylvania Methods for generating new hair follicles, treating baldness and hair removal
US9642789B2 (en) 2005-03-29 2017-05-09 The Trustees Of The University Of Pennsylvania Methods for generating new hair follicles, treating baldness, and hair removal
US9220926B2 (en) 2005-03-29 2015-12-29 The Trustees Of The University Of Pennsylvania Methods for generating new hair follicles, treating baldness, and hair removal
WO2007047707A1 (fr) * 2005-10-17 2007-04-26 Aderans Research Institute, Inc. Procédé consistant à insérer des cellules dans la peau
US9023380B2 (en) 2005-11-22 2015-05-05 Aderans Research Institute, Inc. Hair follicle graft from tissue engineered skin
US8206335B2 (en) 2006-02-09 2012-06-26 Aderans Research Institute, Inc. Apparatus and methods for delivering fluid and material to a subject
US7780635B2 (en) 2006-02-09 2010-08-24 Aderans Research Institute, Inc. Apparatus and methods for delivering fluid and material to a subject
US8980628B2 (en) 2006-03-17 2015-03-17 Aderans Research Institute, Inc. Hair follicle precursor production by co-culturing mammalian dermal papilla cells and keratinocytes
US11207511B2 (en) 2010-12-06 2021-12-28 Follica, Inc. Methods for treating baldness and promoting hair growth

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