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WO1999067373A3 - Invertebrate intestinal mucins, cdnas, antibodies and their use - Google Patents

Invertebrate intestinal mucins, cdnas, antibodies and their use Download PDF

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Publication number
WO1999067373A3
WO1999067373A3 PCT/US1999/014220 US9914220W WO9967373A3 WO 1999067373 A3 WO1999067373 A3 WO 1999067373A3 US 9914220 W US9914220 W US 9914220W WO 9967373 A3 WO9967373 A3 WO 9967373A3
Authority
WO
WIPO (PCT)
Prior art keywords
iim
protein
dna sequence
recombinant
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/014220
Other languages
French (fr)
Other versions
WO1999067373A2 (en
Inventor
Robert R Granados
Ping Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boyce Thompson Institute
Original Assignee
Boyce Thompson Institute for Plant Research Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/103,429 external-priority patent/US6187558B1/en
Application filed by Boyce Thompson Institute for Plant Research Inc filed Critical Boyce Thompson Institute for Plant Research Inc
Priority to CA002331921A priority Critical patent/CA2331921A1/en
Priority to AU47108/99A priority patent/AU4710899A/en
Publication of WO1999067373A2 publication Critical patent/WO1999067373A2/en
Publication of WO1999067373A3 publication Critical patent/WO1999067373A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Insects & Arthropods (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention represents the disclosure of a novel insect intestinal mucin. The IIM protein was been identified and cloned using T. ni larva. The cDNA and amino acid sequences have been determined and are disclosed. The novel protein has an approximate molecular mass of 400 kDa. These sequences are useful for the production of transgenic or recombinant vectors including viral, microorganism, cell, plant, or animals, wherein the virus, microorganism, cell, plant, or animal is the product of an insertion of a gene expression vector including a DNA that encodes an IIM protein sequence. Thereafter the engineered host of the IIM DNA sequence is capable of expressing said IIM protein in a functional form. Also useful is a purified and isolated recombinant DNA sequence comprising a DNA sequence that codes for an IIM protein. The recombinant DNA sequence used can be a cDNA sequence for either IIM14 or IIM22, SEQ. ID.'s No. 1; and 2 respectively. The current invention also provides for the use of the purified amino acid sequences of IIM14 or IIM22, SEQ. ID.'s 3 or 4 respectively. With this knowledge of the proteinaceous components of the PM, and particularly the mucin-like proteins it will be possible to enhance the effectiveness of bio-engineered pesticides, recombinant viral vectors, enhance the defenses of transgenic plants, or protect insect vectors susceptible to attack by organisms utilizing enhancin or enhancin-like enzymes.
PCT/US1999/014220 1998-06-24 1999-06-23 Invertebrate intestinal mucins, cdnas, antibodies and their use Ceased WO1999067373A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002331921A CA2331921A1 (en) 1998-06-24 1999-06-23 A novel invertebrate intestinal mucin cdna and related products and methods
AU47108/99A AU4710899A (en) 1998-06-24 1999-06-23 A novel invertebrate intestinal mucin cdna and related products and methods

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/103,429 1998-06-24
US09/103,429 US6187558B1 (en) 1998-06-24 1998-06-24 Invertebrate intestinal mucin cDNA and related products and methods
US09/294,663 1999-04-19
US09/294,663 US6765127B1 (en) 1998-06-24 1999-04-19 Invertebrate intestinal mucin cDNA and related products and methods

Publications (2)

Publication Number Publication Date
WO1999067373A2 WO1999067373A2 (en) 1999-12-29
WO1999067373A3 true WO1999067373A3 (en) 2000-07-20

Family

ID=26800455

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/014220 Ceased WO1999067373A2 (en) 1998-06-24 1999-06-23 Invertebrate intestinal mucins, cdnas, antibodies and their use

Country Status (3)

Country Link
AU (1) AU4710899A (en)
CA (1) CA2331921A1 (en)
WO (1) WO1999067373A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0108096B1 (en) * 2000-02-04 2017-05-09 Nestle Sa uses of a protein source comprising amino acids wherein the threonine content is at least 5.5% by weight of amino acids
EP3415010A1 (en) 2017-06-13 2018-12-19 Agrosavfe Nv Insect-controlling polypeptides and methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293249A1 (en) * 1987-05-28 1988-11-30 Amrad Corporation Limited Novel fusion protein
WO1996000783A1 (en) * 1994-06-28 1996-01-11 Ciba-Geigy Ag Antibodies which bind to insect gut proteins and their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293249A1 (en) * 1987-05-28 1988-11-30 Amrad Corporation Limited Novel fusion protein
WO1996000783A1 (en) * 1994-06-28 1996-01-11 Ciba-Geigy Ag Antibodies which bind to insect gut proteins and their use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SMITH, M.D.: "Antibody Production in Plants", BIOTECHNOLOGY ADVANCES, vol. 14, no. 3, 1996, pages 267 - 281, XP004046108 *
WANG, P. & GRANADOS, R.R.: "An intestinal mucin is the target substrate for a baculovirus enhancin", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 94, no. 13, 24 June 1997 (1997-06-24), pages 6977 - 6982, XP002125146 *
WANG, P. & GRANADOS, R.R.: "Molecular Cloning and Sequencing of a Novel Invertebrate Intestinal Mucin cDNA", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 26, 27 June 1997 (1997-06-27), pages 16663 - 16669, XP002125145 *

Also Published As

Publication number Publication date
AU4710899A (en) 2000-01-10
CA2331921A1 (en) 1999-12-29
WO1999067373A2 (en) 1999-12-29

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