[go: up one dir, main page]

WO1999061651A3 - Methods for producing a polypeptide by modifying the copy number of a gene - Google Patents

Methods for producing a polypeptide by modifying the copy number of a gene Download PDF

Info

Publication number
WO1999061651A3
WO1999061651A3 PCT/US1999/011816 US9911816W WO9961651A3 WO 1999061651 A3 WO1999061651 A3 WO 1999061651A3 US 9911816 W US9911816 W US 9911816W WO 9961651 A3 WO9961651 A3 WO 9961651A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
polypeptide
acid sequence
cell
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/011816
Other languages
French (fr)
Other versions
WO1999061651A2 (en
Inventor
Debbie S Yaver
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novo Nordisk Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Biotech Inc filed Critical Novo Nordisk Biotech Inc
Priority to JP2000551031A priority Critical patent/JP2002516116A/en
Priority to AU42139/99A priority patent/AU4213999A/en
Priority to EP99925956A priority patent/EP1080210A2/en
Publication of WO1999061651A2 publication Critical patent/WO1999061651A2/en
Publication of WO1999061651A3 publication Critical patent/WO1999061651A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant cell under conditions conducive for production of the polypeptide, wherein (i) the mutant cell is related to a parent cell, which parent cell comprises at least two tandem copies of a nucleic acid sequence encoding the polypeptide or a nucleic acid sequence encoding the polypeptide, which nucleic acid sequence comprises repeat sequences at the 5' and 3' ends of the nucleic acid sequence, by the introduction of a nucleic acid construct into the genome of the parent cell at a locus which is within or not within the nucleic acid sequence(s), wherein the introduction of the nucleic acid construct into the locus modifies the copy number of the nucleic acid sequence(s) and the modification of the copy number is not a result of selective pressure; and (ii) the mutant cell produces more or less of the polypeptide than the parent cell when both cells are cultivated under the same conditions; and (b) recovering the poylpeptide from the cultivation medium. The present invention also relates to methods for obtaining a mutant cell and mutant cells.
PCT/US1999/011816 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene Ceased WO1999061651A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2000551031A JP2002516116A (en) 1998-05-27 1999-05-27 Method for producing a polypeptide by changing the copy number of a gene
AU42139/99A AU4213999A (en) 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene
EP99925956A EP1080210A2 (en) 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8550598A 1998-05-27 1998-05-27
US09/085,505 1998-05-27

Publications (2)

Publication Number Publication Date
WO1999061651A2 WO1999061651A2 (en) 1999-12-02
WO1999061651A3 true WO1999061651A3 (en) 2000-03-02

Family

ID=22192064

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/011816 Ceased WO1999061651A2 (en) 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene

Country Status (5)

Country Link
EP (1) EP1080210A2 (en)
JP (1) JP2002516116A (en)
CN (1) CN1307642A (en)
AU (1) AU4213999A (en)
WO (1) WO1999061651A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000046375A2 (en) * 1999-02-02 2000-08-10 Novozymes Biotech, Inc. Methods for producing polypeptides in fungal cells
CN1312260A (en) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 Human proteinase regulating protein 10 as one new polypeptide and polynucleotides encoding this polypeptide
US20020164613A1 (en) * 2000-11-06 2002-11-07 Villarete Lorelie H. Method of determining gene copy number
DE60331644D1 (en) 2002-04-22 2010-04-22 Novozymes Inc METHOD FOR PRODUCING VARIANTS OF A DNA SEQUENCE IN FILAMENTOUS MUSHROOMS
ATE471370T1 (en) 2003-05-02 2010-07-15 Novozymes Inc VARIANTS OF BETA-GLUCOSIDASES
BRPI0413830A (en) * 2003-08-22 2006-10-24 Suntory Ltd method for the reproduction of lipid producing fungi
EP2377931B1 (en) 2003-08-25 2013-05-08 Novozymes Inc. Variants of glycoside hydrolases
CN100543141C (en) * 2003-09-16 2009-09-23 周琪 Method for producing gene engineering recombination 192 peptide human growth hormone
CN1902310B (en) 2003-10-28 2011-09-21 诺维信股份有限公司 Polypeptides with β-glucosidase activity and polynucleotides encoding said polypeptides
ES2357037T3 (en) 2004-01-16 2011-04-15 Novozymes, Inc. METHODS FOR DEGRADING LIGNOCELLULOSIC MATERIALS.
DK2305702T3 (en) 2004-01-30 2014-06-16 Novozymes Inc Polypeptides with cellulolytic enhancing activity and polynucleotides encoding them
CN1993475A (en) 2004-05-27 2007-07-04 诺维信股份有限公司 Methods for transforming and expression screening of filamentous fungal cells with a DNA library
CA2606475C (en) 2005-04-27 2015-06-16 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
NZ566984A (en) 2005-09-30 2011-12-22 Novozymes Inc Methods for enhancing the degradation or conversion of cellulosic material
EP2004817A2 (en) 2006-03-30 2008-12-24 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
FR2927089B1 (en) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra METHOD OF TARGETED INTEGRATION OF MULTICOPIES OF A GENE OF INTEREST IN A YARROWIA STRAIN
EP2358878B1 (en) 2008-11-20 2014-10-15 Novozymes Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
US8338121B2 (en) 2008-12-19 2012-12-25 Novozymes, Inc. Methods for determining cellulolytic enhancing activity of a polypeptide
WO2010088387A1 (en) 2009-01-28 2010-08-05 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2010106068A1 (en) 2009-03-17 2010-09-23 Novozymes A/S Polypeptides having tyrosinase activity and polynucleotides encoding same
EP2491122A1 (en) 2009-10-23 2012-08-29 Novozymes Inc. Cellobiohydrolase variants and polynucleotides encoding same
DK2496693T3 (en) 2009-11-06 2018-01-22 Novozymes Inc Polypeptides with cellobiohydrolase activity and polynucleotides encoding them
ES2574054T3 (en) 2009-11-06 2016-06-14 Novozymes, Inc. Polypeptides with xylanase activity and polynucleotides that encode them
EP2496694B1 (en) 2009-11-06 2017-04-19 Novozymes, Inc. Compositions for saccharification of cellulosic material
CN107345226A (en) 2010-09-30 2017-11-14 诺维信股份有限公司 Polypeptide variants and its coded polynucleotide with cellulolytic enhancing activity
BR112013007693B1 (en) 2010-09-30 2021-12-07 Novozymes, Inc VARIANT, ISOLATED POLYNUCLEOTIDE, RECOMBINANT MICROBIAL HOST CELL, METHODS FOR PRODUCING A VARIANT, TO PRODUCE A VARIANT HAVING CELLULOLYTIC ENHANCEMENT ACTIVITY, TO DEGRADE A CELLULOSIC MATERIAL, TO PRODUCE A FERMENTATION PRODUCT, TO FERMENT A CELLULOSIC, AND ELIGIBLE MATERIAL. COMPLETE BROTH OR CELL CULTURE COMPOSITION
ES2563209T3 (en) 2010-10-01 2016-03-11 Novozymes, Inc. Variants of beta-glucosidase and polynucleotides that encode them
BR112013010129A2 (en) 2010-11-18 2016-07-05 Novozymes Inc isolated chimeric gh61 polypeptide, isolated polynucleotide, host cell, methods for producing a chimeric gh61 polypeptide, for degrading or converting a cellulostic material, for synthesizing a fermentation product, for fermenting a cellulosic material and for cleaning or washing a hard surface or clothing for washing, transgenic plant, part of plant or plant cell, use of chimeric gh61 polypeptide, detergent composition, and broth formulation or whole cell culture composition
WO2012103350A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2691519A1 (en) 2011-03-31 2014-02-05 Novozymes, Inc. Cellulose binding domain variants and polynucleotides encoding same
US9926547B2 (en) 2011-04-28 2018-03-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
MX2013011827A (en) 2011-04-29 2014-01-08 Novozymes Inc Methods for enhancing the degradation or conversion of cellulosic material.
BR112014007651A2 (en) 2011-09-30 2017-04-11 Novozymes Inc isolated chimeric polypeptide, isolated polynucleotide, methods for producing a chimeric polypeptide and a fermentation product, for degrading or converting a cellulosic material, and for fermenting a transgenic cellulosic material, plant, plant part or cell, and broth formulation whole or cell culture composition
BR112014015228B1 (en) 2011-12-20 2022-07-05 Novozymes, Inc. GENITOR CELOBIOHIDROLASE VARIANT, PROCESSES FOR DEGRADING OR CONVERTING A CELLULOSIC MATERIAL, FOR PRODUCTION OF A FERMENTATION PRODUCT, AND FOR FERMENTATION OF A CELLULOSIC MATERIAL, WHOLE BROTH FORMULATION OR CELL CULTURE COMPOSITION, AND, TRANSGENIC MICROBIAN HOST CELL
EP4363598A4 (en) * 2021-06-29 2025-09-17 Novozymes As Process for the production of fermentation products using fiber-degrading enzymes in fermentation

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533A1 (en) * 1984-04-27 1985-10-31 Centre Nat Rech Scient METHOD FOR AMPLIFYING EXPRESSION OF A GENE DETERMINED IN BACILLUS SUBTILIS AND OBTAINED STRAINS
WO1988003169A1 (en) * 1986-10-27 1988-05-05 Whitehead Institute For Biomedical Research Gene amplification using retrotransposons
EP0343783A2 (en) * 1988-04-22 1989-11-29 Btg Usa Inc Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
WO1991009955A1 (en) * 1989-12-22 1991-07-11 Applied Research Systems Ars Holding N.V. Endogenous gene expression modification with regulatory element
WO1992001800A1 (en) * 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
WO1995031560A1 (en) * 1994-05-13 1995-11-23 Transkaryotic Therapies, Inc. Dna construct for effecting homologous recombination and uses thereof
WO1998011203A1 (en) * 1996-09-13 1998-03-19 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533A1 (en) * 1984-04-27 1985-10-31 Centre Nat Rech Scient METHOD FOR AMPLIFYING EXPRESSION OF A GENE DETERMINED IN BACILLUS SUBTILIS AND OBTAINED STRAINS
WO1988003169A1 (en) * 1986-10-27 1988-05-05 Whitehead Institute For Biomedical Research Gene amplification using retrotransposons
EP0343783A2 (en) * 1988-04-22 1989-11-29 Btg Usa Inc Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
WO1991009955A1 (en) * 1989-12-22 1991-07-11 Applied Research Systems Ars Holding N.V. Endogenous gene expression modification with regulatory element
WO1992001800A1 (en) * 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
WO1995031560A1 (en) * 1994-05-13 1995-11-23 Transkaryotic Therapies, Inc. Dna construct for effecting homologous recombination and uses thereof
WO1998011203A1 (en) * 1996-09-13 1998-03-19 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide

Also Published As

Publication number Publication date
JP2002516116A (en) 2002-06-04
WO1999061651A2 (en) 1999-12-02
AU4213999A (en) 1999-12-13
CN1307642A (en) 2001-08-08
EP1080210A2 (en) 2001-03-07

Similar Documents

Publication Publication Date Title
WO1999061651A3 (en) Methods for producing a polypeptide by modifying the copy number of a gene
WO2000056900A3 (en) Promoter sequences derived from fusarium venenatum and uses thereof
CN106460000B (en) Targeted mutagenesis
KR920701449A (en) Α-amylase of variant microorganisms with increased stability to temperature, acid and / or alkali
NZ335392A (en) Method for the recombinant production of 1,3-propanediol
WO1999043835A3 (en) Methods for producing a polypeptide in a bacillus cell
IL162302A (en) Methods of producing hyaluronic acid in a bacillus host cell
EP1613761A4 (en) Methods for producing biological substances in enzyme-deficient mutants of aspergillus niger
ATE414782T1 (en) METHOD FOR PRODUCING POLYPEPTIDES IN MUTATED ASPERGILLUS CELLS
EP0506945A4 (en) Method of gene transfer using retrotransposons
AU3307797A (en) A method for increasing hemoprotein production in filamentous fungi
RU95115686A (en) PREMERSACIDINE, PROMERSACIDINE, DNA, VECTOR, HOST CELL, METHOD FOR PRODUCING
DE69938722D1 (en) METHOD FOR THE PRODUCTION OF HETEROLOGIC POLYPEPTIDES IN TRICHOTHECENE LACK OF FILAMENT SOLVENTS MUSHROOMS
AU4940096A (en) Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
NZ507607A (en) Method of producing a transgenic plant by transforming witha 1-SST enzyme (sucrose:surcose 1-fructosyltransferase) and/or a 1-FFT enzyme (fructan:fructan 1-fructosyltransferase)
WO1994000463A3 (en) Production of hyaluronic acid by transeformed microorganisms
WO2004011609A3 (en) Methods for producing biological substances in pigment-deficient mutants of bacillus cells
EP2298911A3 (en) Methods for producing polypeptides in respiratory-deficient cells
WO2000046375A3 (en) Methods for producing polypeptides in fungal cells
NO20052209L (en) Excellence for the production of new and natural avermectin
RU99119890A (en) STREPTOMYCES AVERMITILIS REGULATORY GENES, ENSURING AN INCREASED PRODUCTION OF AVERMECTIN
JPH05260979A (en) Production of d-ribose
JP2002539834A5 (en)
WO2001051646A3 (en) Methods for producing a polypeptide using a crippled translational initiator sequence
CA2394792A1 (en) Promoter and intron from maize actin depolymerizing factor

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99808098.5

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AU BA BB BG BR CA CN CU CZ EE GD GE HR HU ID IL IN IS JP KP KR LC LK LR LT LV MG MK MN MX NO NZ PL RO SG SI SK SL TR TT UA UZ VN YU ZA

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AU BA BB BG BR CA CN CU CZ EE GD GE HR HU ID IL IN IS JP KP KR LC LK LR LT LV MG MK MN MX NO NZ PL RO SG SI SK SL TR TT UA UZ VN YU ZA

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1999925956

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2000 551031

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: KR

WWP Wipo information: published in national office

Ref document number: 1999925956

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999925956

Country of ref document: EP