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WO1999061653A2 - Procede et appareil d'analyse photoinduite par zones de materiaux biologiques - Google Patents

Procede et appareil d'analyse photoinduite par zones de materiaux biologiques Download PDF

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Publication number
WO1999061653A2
WO1999061653A2 PCT/US1999/011910 US9911910W WO9961653A2 WO 1999061653 A2 WO1999061653 A2 WO 1999061653A2 US 9911910 W US9911910 W US 9911910W WO 9961653 A2 WO9961653 A2 WO 9961653A2
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WIPO (PCT)
Prior art keywords
biologic material
photoresist
region
coated
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/011910
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English (en)
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WO1999061653A3 (fr
Inventor
John A. Zebala
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syntrix Biosystems Inc
Original Assignee
Syntrix Biochip Inc
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Filing date
Publication date
Application filed by Syntrix Biochip Inc filed Critical Syntrix Biochip Inc
Priority to AU42184/99A priority Critical patent/AU4218499A/en
Publication of WO1999061653A2 publication Critical patent/WO1999061653A2/fr
Publication of WO1999061653A3 publication Critical patent/WO1999061653A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
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Definitions

  • Microdissection using a dissecting microscope has been described providing for better isolation of selected material (see Emmert-Buck et al., Am. J. Pathol. 1-15:1285, 1994; Zhuang et al., Am. J. Pathol 146:620, 1995 and Noguchi et al., Cancer Res. 54:1849, 1994).
  • microdissection is highly labor intensive and difficult to automate.
  • Step (b) may be performed using any of a variety of analytical techniques, such as those in which the exposed biologic material is contacted with a detection reagent that generates a detectable response indicative of the presence or absence of a substance of interest in the exposed biologic material; and a response generated by the detection reagent is detected.
  • Detection reagents may comprise, for example, one or more of an antibody, nucleic acid sequence or enzyme and/or a marker such as a chromophor, fluorophor or radionuclide.
  • Other analytical techniques include electrophoresis, chromatography, mass spectrometry. DNA sequencing, peptide sequencing, nucleic acid hybridization and PCR.
  • the applied irradiation may be, for example, coherent light, incoherent light, x-ray light, ultraviolet light, visible light or infrared light.
  • Figures 11 A and 1 IB are photographs demonstrating specificity of target selection.
  • Figure 1 1 A shows destained male lymphocytes.
  • Figure 1 IB shows the cells after the addition of extraction solution. Irradiated regions are indicated by the three roughly circular regions.
  • Figure 19 is an autoradiogram demonstrating a microsatellite analysis of DCIS and cancer according to the present invention.
  • Cells were characterized as normal (N), DCIS (D) or cancer (C) as indicated.
  • PCR analyses were performed for the indicated microsatellite marker for the methyl CG binding protein 2 (MECP2) and a cytogenetic region involved in choosing which X chromosome is inactivated (Xq27.3).
  • MECP2 methyl CG binding protein 2
  • Xq27.3 a cytogenetic region involved in choosing which X chromosome is inactivated
  • the differential between the intensity of light transmitted by substantially transparent and substantially opaque regions as a percentage of the intensity of light transmitted by substantially transparent regions should be greater than 75%, more preferably greater than 90%. and most preferably greater than 99%. Importantly, the differential should be sufficient to permit irradiation of photoresist as defined above.
  • the entire substrate was immersed in AZ® 351 developer diluted six- fold with distilled water (Hoechst CelaneseTM, Somerville, NJ).
  • the photoresist in irradiated regions was completely dissolved after about 30 seconds in developer.
  • the temporal progress of dissolution was visually monitored by the formation of red dye from irradiated regions during the development process.
  • the dye is thought to form by the reaction of indene carboxylic acid (IV) with unreacted diazoquinone.
  • the biologic material in irradiated regions lost both hematoxylin and eosin stains, appearing clear and translucent to the naked eye.

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Abstract

L'invention porte sur des compositions et méthodes d'analyse par zones de matériaux biologiques. Lesdites méthodes utilisent une couche de photorésist disposée sur un matériau biologique (éventuellement immobilisé sur un substrat). Les zones d'intérêt sont sélectionnées, puis irradiées en exposant certaine parties spécifiques du matériau biologique. Le matériau biologique exposé peut ensuite être soumis à une analyse sélective recourant à diverses méthodes d'analyse.
PCT/US1999/011910 1998-05-27 1999-05-27 Procede et appareil d'analyse photoinduite par zones de materiaux biologiques Ceased WO1999061653A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU42184/99A AU4218499A (en) 1998-05-27 1999-05-27 Light-mediated method and apparatus for the regional analysis of biologic material

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US8530298A 1998-05-27 1998-05-27
US09/085,302 1998-05-27
US11052998P 1998-12-01 1998-12-01
US11052898P 1998-12-01 1998-12-01
US11052798P 1998-12-01 1998-12-01
US60/110,529 1998-12-01
US60/110,528 1998-12-01
US60/110,527 1998-12-01

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WO1999061653A2 true WO1999061653A2 (fr) 1999-12-02
WO1999061653A3 WO1999061653A3 (fr) 2000-04-20

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Cited By (10)

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EP1243327A1 (fr) * 2001-03-19 2002-09-25 BioChip Technologies GmbH Surface avec un motif de cellules et méthode pour sa production
EP1105525A4 (fr) * 1999-06-07 2003-07-30 Samsung Electronics Co Ltd Procede d'elaboration de sonde d'acide nucleique peptidique au moyen d'un generateur de photoacide polymere
US6692914B1 (en) 1999-07-02 2004-02-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate, where the polymers have water-soluble or water-dispersible segments and probes bonded thereto
WO2005065814A1 (fr) * 2004-01-07 2005-07-21 Solexa Limited Arrangements moleculaires modifies
EP1655069A1 (fr) * 2004-08-05 2006-05-10 Solexa Limited Réseaux moléculaires modifiés
US7205161B2 (en) 2001-01-10 2007-04-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate having improved stability
US7273633B2 (en) * 2003-12-22 2007-09-25 Stmicroelectronics Ltd. Sensors
ITTO20100577A1 (it) * 2010-07-05 2012-01-06 Univ Degli Studi Genova Supporto planare avente una superficie ultrapiatta e dispositivo per la rivelazione di antigeni comprendente detto supporto planare
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
CN112469852A (zh) * 2018-04-14 2021-03-09 生捷科技控股公司 用于捕获dna分子的dna桥方法

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US4415405A (en) * 1981-08-19 1983-11-15 Yale University Method for engraving a grid pattern on microscope slides and slips
DE69013764T2 (de) * 1989-06-03 1995-03-30 Kanegafuchi Chemical Ind Kontrolle der Zellanordnung.
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JP2609556B2 (ja) * 1990-06-08 1997-05-14 株式会社バイオマテリアル研究所 神経細胞の培養に用いる基質

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* Cited by examiner, † Cited by third party
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EP1105525A4 (fr) * 1999-06-07 2003-07-30 Samsung Electronics Co Ltd Procede d'elaboration de sonde d'acide nucleique peptidique au moyen d'un generateur de photoacide polymere
US6833276B2 (en) 1999-07-02 2004-12-21 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface and having water-soluble or water-dispersible segments therein and probes bonded thereto
US6692914B1 (en) 1999-07-02 2004-02-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate, where the polymers have water-soluble or water-dispersible segments and probes bonded thereto
US7205161B2 (en) 2001-01-10 2007-04-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate having improved stability
EP1243327A1 (fr) * 2001-03-19 2002-09-25 BioChip Technologies GmbH Surface avec un motif de cellules et méthode pour sa production
WO2002074434A3 (fr) * 2001-03-19 2003-10-30 Genovac Ag Surface comportant un motif de cellules et procede de fabrication de cette surface
US7273633B2 (en) * 2003-12-22 2007-09-25 Stmicroelectronics Ltd. Sensors
WO2005065814A1 (fr) * 2004-01-07 2005-07-21 Solexa Limited Arrangements moleculaires modifies
US11654411B2 (en) 2004-01-07 2023-05-23 Illumina Cambridge Limited Methods and compositions of localizing nucleic acids to arrays
US10953379B2 (en) 2004-01-07 2021-03-23 Illumina Cambridge Limited Methods and compositions of localizing nucleic acids to arrays
US8563477B2 (en) 2004-01-07 2013-10-22 Illumina Cambridge Limited Modified molecular arrays
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