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WO1999058948A3 - Detection d'analyte selon un procede de numeration - Google Patents

Detection d'analyte selon un procede de numeration Download PDF

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Publication number
WO1999058948A3
WO1999058948A3 PCT/US1999/010917 US9910917W WO9958948A3 WO 1999058948 A3 WO1999058948 A3 WO 1999058948A3 US 9910917 W US9910917 W US 9910917W WO 9958948 A3 WO9958948 A3 WO 9958948A3
Authority
WO
WIPO (PCT)
Prior art keywords
signal
sample
analyte
enumeration
binding events
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/010917
Other languages
English (en)
Other versions
WO1999058948A2 (fr
Inventor
Timothy W Starzl
Scott Clark
Marybeth Robinson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DDX Inc
Original Assignee
DDX Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DDX Inc filed Critical DDX Inc
Priority to CA002331787A priority Critical patent/CA2331787A1/fr
Priority to NZ508062A priority patent/NZ508062A/en
Priority to IL13963199A priority patent/IL139631A0/xx
Priority to JP2000548701A priority patent/JP2002526743A/ja
Priority to EP99925655A priority patent/EP1188059A2/fr
Priority to AU41900/99A priority patent/AU4190099A/en
Publication of WO1999058948A2 publication Critical patent/WO1999058948A2/fr
Anticipated expiration legal-status Critical
Publication of WO1999058948A3 publication Critical patent/WO1999058948A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Nanotechnology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Materials Engineering (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne un procédé et un système optiques permettant de détecter un analyte par une immobilisation en phase solide, des marqueurs d'analyte spécifiques aptes à produire un signal et des procédés d'utilisation de ces derniers. Le procédé de détection par numération de l'invention permet de rétrécir la zone d'observation du signal et, par conséquent, d'améliorer le rapport signal détectable/fond. Le système comprend une plate-forme/support permettant d'immobiliser une platine d'échantillon sur laquelle est lié un échantillon marqué (complexe d'analyte), une source de rayonnement, un appareil optique destiné à générer et à diriger le rayonnement vers l'échantillon et un moyen de collecte et d'analyse des données. Au moment où le système est enclenché, l'échantillon génère un signal pouvant se différencier du signal de fond, les deux signaux étant collectés et mis en image grâce à un détecteur de signal qui transmet une image de l'échantillon à un appareil de traitement des données. Ledit appareil reçoit les mesures du signal et, à son tour, effectue la numération des événements de liaison individuels. Il est possible de renforcer le signal généré par une augmentation choisie de la masse. Le procédé d'analyse par numération de l'invention, qui permet de détecter des événements de liaison individuels, peut être utilisé, par exemple, dans des analyses visant à détecter un analyte ou à confirmer des résultats dans des applications de recherche, des applications commerciales ou aux endroits où des soins sont dispensés.
PCT/US1999/010917 1998-05-13 1999-05-13 Detection d'analyte selon un procede de numeration Ceased WO1999058948A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA002331787A CA2331787A1 (fr) 1998-05-13 1999-05-13 Detection d'analyte selon un procede de numeration
NZ508062A NZ508062A (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection
IL13963199A IL139631A0 (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection
JP2000548701A JP2002526743A (ja) 1998-05-13 1999-05-13 アナライト検出算出法
EP99925655A EP1188059A2 (fr) 1998-05-13 1999-05-13 Detection d'analyte selon un procede de numeration
AU41900/99A AU4190099A (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8525998P 1998-05-13 1998-05-13
US60/085,259 1998-05-13

Publications (2)

Publication Number Publication Date
WO1999058948A2 WO1999058948A2 (fr) 1999-11-18
WO1999058948A3 true WO1999058948A3 (fr) 2002-01-03

Family

ID=22190457

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/010917 Ceased WO1999058948A2 (fr) 1998-05-13 1999-05-13 Detection d'analyte selon un procede de numeration

Country Status (7)

Country Link
EP (1) EP1188059A2 (fr)
JP (1) JP2002526743A (fr)
AU (1) AU4190099A (fr)
CA (1) CA2331787A1 (fr)
IL (1) IL139631A0 (fr)
NZ (1) NZ508062A (fr)
WO (1) WO1999058948A2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895255B1 (en) 2003-07-12 2014-11-25 Accelerate Diagnostics, Inc. Sensitive and rapid determination of antimicrobial susceptibility
US9057046B2 (en) 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
US9290382B2 (en) 2001-09-06 2016-03-22 Rapid Micro Biosystems Rapid detection of replicating cells
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001227112B2 (en) * 2000-01-31 2006-06-15 Panasonic Ecology Systems Co., Ltd. Kit for Detecting Microorganisms, Apparatus for Quantifying Microorganisms and Method for Quantifying Microorganisms
GB0029154D0 (en) 2000-11-30 2001-01-17 Lee Helen Signal enhancement with multiple labelled-antibodies
DE10113711A1 (de) * 2001-03-16 2002-09-26 Lifebits Ag Verfahren zum Nachweis biochemischer Reaktionen und Messträger zur Durchführung des Verfahrens
JP3945636B2 (ja) * 2001-03-27 2007-07-18 富士フイルム株式会社 測定装置
US20120077206A1 (en) 2003-07-12 2012-03-29 Accelr8 Technology Corporation Rapid Microbial Detection and Antimicrobial Susceptibility Testing
US8460878B2 (en) 2006-02-21 2013-06-11 The Trustees Of Tufts College Methods and arrays for detecting cells and cellular components in small defined volumes
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
CA2734029C (fr) 2007-08-30 2016-03-29 The Trustees Of Tufts College Procedes pour determiner la concentration d'un analyte dans une solution
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
US11865534B2 (en) 2008-09-24 2024-01-09 First Light Diagnostics, Inc. Imaging analyzer for testing analytes
US9339813B2 (en) 2009-12-18 2016-05-17 Koninklijke Philips N.V. Substance determining apparatus
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9678068B2 (en) 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
EP2542890B1 (fr) 2010-03-01 2015-05-06 Quanterix Corporation Procédés pour étendre la plage dynamique dans des dosages pour la détection de molécules ou de particules
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
CN103492859B (zh) * 2011-02-28 2017-05-24 皇家飞利浦有限公司 物质确定装置
US10254204B2 (en) 2011-03-07 2019-04-09 Accelerate Diagnostics, Inc. Membrane-assisted purification
US20140302532A1 (en) 2011-04-12 2014-10-09 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury
EP2776550B1 (fr) 2011-11-07 2018-01-10 Rapid Micro Biosystems, Inc. Cassette pour test de stérilité
CN104364388B (zh) 2012-04-16 2017-12-19 快速微型生物系统公司 细胞培养装置
WO2014113502A1 (fr) 2013-01-15 2014-07-24 Quanterix Corporation Détection de l'adn ou de l'arn au moyen de matrices de molécules simples et d'autres techniques
US9677109B2 (en) 2013-03-15 2017-06-13 Accelerate Diagnostics, Inc. Rapid determination of microbial growth and antimicrobial susceptibility
US10253355B2 (en) 2015-03-30 2019-04-09 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
US10023895B2 (en) 2015-03-30 2018-07-17 Accelerate Diagnostics, Inc. Instrument and system for rapid microogranism identification and antimicrobial agent susceptibility testing
CN119804399A (zh) 2018-04-19 2025-04-11 曙光诊断学公司 靶标检测
EP3860452A4 (fr) 2018-10-04 2022-11-23 First Light Diagnostics, Inc. Instrument d'analyse

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4508832A (en) * 1981-06-22 1985-04-02 Battelle Memorial Institute Ellipsometrically measuring rate of optical change in immunoassay
US4521522A (en) * 1981-09-05 1985-06-04 Kurt I. Lundstroem Optical specific binding assay with reflection of polarized electromagnetic radiation
US4805623A (en) * 1987-09-04 1989-02-21 Vander Corporation Spectrophotometric method for quantitatively determining the concentration of a dilute component in a light- or other radiation-scattering environment
US4876208A (en) * 1987-01-30 1989-10-24 Yellowstone Diagnostics Corporation Diffraction immunoassay apparatus and method
US4885077A (en) * 1988-11-17 1989-12-05 Becton, Dickinson And Company Composite membrane, method for its preparation and electrolyte sensor including same
US5017009A (en) * 1986-06-26 1991-05-21 Ortho Diagnostic Systems, Inc. Scattered total internal reflectance immunoassay system
US5496701A (en) * 1991-06-04 1996-03-05 Fisons Plc Optical biosensor method for determining an analyte
US5622868A (en) * 1989-04-27 1997-04-22 Microbiological Research Authority Camr (Centre For Applied Microbiology & Research) Analytical apparatus utilizing a colorimetric or other optically detectable effect

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4508832A (en) * 1981-06-22 1985-04-02 Battelle Memorial Institute Ellipsometrically measuring rate of optical change in immunoassay
US4521522A (en) * 1981-09-05 1985-06-04 Kurt I. Lundstroem Optical specific binding assay with reflection of polarized electromagnetic radiation
US5017009A (en) * 1986-06-26 1991-05-21 Ortho Diagnostic Systems, Inc. Scattered total internal reflectance immunoassay system
US4876208A (en) * 1987-01-30 1989-10-24 Yellowstone Diagnostics Corporation Diffraction immunoassay apparatus and method
US4805623A (en) * 1987-09-04 1989-02-21 Vander Corporation Spectrophotometric method for quantitatively determining the concentration of a dilute component in a light- or other radiation-scattering environment
US4885077A (en) * 1988-11-17 1989-12-05 Becton, Dickinson And Company Composite membrane, method for its preparation and electrolyte sensor including same
US5622868A (en) * 1989-04-27 1997-04-22 Microbiological Research Authority Camr (Centre For Applied Microbiology & Research) Analytical apparatus utilizing a colorimetric or other optically detectable effect
US5496701A (en) * 1991-06-04 1996-03-05 Fisons Plc Optical biosensor method for determining an analyte

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9290382B2 (en) 2001-09-06 2016-03-22 Rapid Micro Biosystems Rapid detection of replicating cells
US8895255B1 (en) 2003-07-12 2014-11-25 Accelerate Diagnostics, Inc. Sensitive and rapid determination of antimicrobial susceptibility
US9057046B2 (en) 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems

Also Published As

Publication number Publication date
CA2331787A1 (fr) 1999-11-18
WO1999058948A2 (fr) 1999-11-18
AU4190099A (en) 1999-11-29
JP2002526743A (ja) 2002-08-20
IL139631A0 (en) 2002-02-10
NZ508062A (en) 2002-04-26
EP1188059A2 (fr) 2002-03-20

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