[go: up one dir, main page]

WO1999052941B1 - Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses - Google Patents

Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses

Info

Publication number
WO1999052941B1
WO1999052941B1 PCT/GB1999/001096 GB9901096W WO9952941B1 WO 1999052941 B1 WO1999052941 B1 WO 1999052941B1 GB 9901096 W GB9901096 W GB 9901096W WO 9952941 B1 WO9952941 B1 WO 9952941B1
Authority
WO
WIPO (PCT)
Prior art keywords
rho
proteins
gtpases
assays
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1999/001096
Other languages
English (en)
Other versions
WO1999052941A3 (fr
WO1999052941A2 (fr
Inventor
Leland Herries Johnston
Richard Henry Treisman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Cancer Research Horizons Ltd
Original Assignee
Imperial Cancer Research Technology Ltd
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial Cancer Research Technology Ltd, Medical Research Council filed Critical Imperial Cancer Research Technology Ltd
Priority to EP99915903A priority Critical patent/EP1068234A2/fr
Priority to AU34326/99A priority patent/AU3432699A/en
Publication of WO1999052941A2 publication Critical patent/WO1999052941A2/fr
Publication of WO1999052941A3 publication Critical patent/WO1999052941A3/fr
Publication of WO1999052941B1 publication Critical patent/WO1999052941B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La famille Rho des GTPases est impliquée dans de nombreux processus cellulaires eukaryotes, certaines protéines effectrices étant connues pour se fixer à ces GTPases, afin de provoquer différents effets, ces protéines étant notamment la ROCK-I/p160ROCK, la ROCKII, et la kinectine. La présente invention concerne également une nouvelle région de fixation à Rho à vingt-trois acides aminés, conservée dans un éventail de protéines de fixation à Rho. D'autres protéines, notamment la protéine de levure Skn7, contiennent des régions homologues à cette région conservée et interagissent avec les Rho GTPases. L'invention concerne enfin des analyses permettant d'identifier les molécules qui altèrent la croissance cellulaire, par une modulation de l'interaction Rho GTPase/protéines effectrices.
PCT/GB1999/001096 1998-04-09 1999-04-09 Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses Ceased WO1999052941A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP99915903A EP1068234A2 (fr) 1998-04-09 1999-04-09 Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses
AU34326/99A AU3432699A (en) 1998-04-09 1999-04-09 Polypeptide domains capable of binding rho protein and their use in assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9807848.8A GB9807848D0 (en) 1998-04-09 1998-04-09 Assay method and means
GB9807848.8 1998-04-09

Publications (3)

Publication Number Publication Date
WO1999052941A2 WO1999052941A2 (fr) 1999-10-21
WO1999052941A3 WO1999052941A3 (fr) 1999-12-02
WO1999052941B1 true WO1999052941B1 (fr) 2000-01-06

Family

ID=10830272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001096 Ceased WO1999052941A2 (fr) 1998-04-09 1999-04-09 Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses

Country Status (4)

Country Link
EP (1) EP1068234A2 (fr)
AU (1) AU3432699A (fr)
GB (1) GB9807848D0 (fr)
WO (1) WO1999052941A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1088898A1 (fr) * 1999-09-16 2001-04-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Un nouveau système de détection de substances utiles dans la thérapie du cancer et des maladies infectieuses
AU2001269078A1 (en) * 2000-06-19 2002-01-02 Boehringer Ingelheim International G.M.B.H Cyk-4 polypeptides, dna molecules encoding them and their use in screening methods
EP1254912A1 (fr) * 2001-04-30 2002-11-06 Boehringer Ingelheim International GmbH Polypeptides de CYK-4, ADN molecules condant et leur utilisation pour de procédé de criblage
CA2513148A1 (fr) * 2003-01-23 2004-08-05 Eirx Therapeutics Limited Kinases et recepteurs gpcr impliques dans l'apoptose
FR2862983B1 (fr) * 2003-11-28 2006-04-07 Centre Nat Rech Scient Moyens de criblage pour l'identification de regulateurs de l'activite gtpase

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5519003A (en) * 1994-02-01 1996-05-21 Board Of Trustees Of The Leland Stanford Junior University WD-40-derived peptides and uses thereof
CA2228374A1 (fr) * 1995-07-31 1997-02-13 Charles R. Vinson Prolongement d'une surface d'interaction proteine-proteine afin d'inactiver le fonctionnement d'une proteine cellulaire
US6238881B1 (en) * 1996-11-06 2001-05-29 Onyx Pharmaceuticals, Inc. Nucleic acids and polypeptides related to a guanine exchange factor of Rho GTPase
GB9704162D0 (en) * 1997-02-28 1997-04-16 Medical Res Council Assay methods and means

Also Published As

Publication number Publication date
AU3432699A (en) 1999-11-01
WO1999052941A3 (fr) 1999-12-02
GB9807848D0 (en) 1998-06-10
WO1999052941A2 (fr) 1999-10-21
EP1068234A2 (fr) 2001-01-17

Similar Documents

Publication Publication Date Title
AU7542398A (en) Nucleic acid binding proteins
AU9174398A (en) Peptides
EP2308974A3 (fr) Chromo/fluoroprotéines dérivées d'anthozoaires et leurs procédés d'utilisation
WO2003020932A1 (fr) Nouvelles proteines de secretion et adn associe
NO962991D0 (no) Zink-finger-proteinderivater og fremgangsmåter
NZ323795A (en) Isolated nucleic acid molecules encoding AUR-1 (aurora-1) and AUR-2 polypeptides
DE69731479T2 (de) Von filaggrin abgeleitete antigene und deren anwendung in der diagnose von rheumatischer polyarthritis
WO1994017097B1 (fr) Fragments de fibronectine synthetique utilises comme inhibiteurs d'infections retrovirales
CA2068190A1 (fr) Methodes et compositions pour des essais enzymatiques complementaires faisant appel a la reaction omega de la beta-galactosidase
GB9929140D0 (en) Diagnostic assay for stroke
AU8623798A (en) Smad-interacting polypeptides and their use
WO1999052941B1 (fr) Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses
ATE208815T1 (de) Genetisches igfbp-5 rodierendes material
WO1994026891A3 (fr) Ligands purifies de flt3 de mammiferes et leurs agonistes/antagonistes
BR0213779A (pt) Molécula de ácido nucleico isolada, vetor, célula hospedeira, e, método para a preparação de um composto de quìmica fina
Yamada et al. An S-alkylating reagent with positive charges as an efficient solubilizer of denatured disulfide-containing proteins
EP1132397A4 (fr) Proteine de detection et utilisation de celle-ci
Tucker et al. Crystallization of the C-terminal domain of colicin A carrying the voltage-dependent pore activity of the protein
CA2025929A1 (fr) Methode d'essais de complementation enzymatique de proteines de liaison
DE69925116D1 (de) Neue, physiologisch aktive peptide und ihre verwendung.
Subbalakshmi et al. Biological activities of retro and diastereo analogs of a 13‐residue peptide with antimicrobial and hemolytic activities: Authors' affiliations
Pope et al. Loss of calcium sensitivity of plasma gelsolin is associated with the presence of calcium ions during preparation
Lampen et al. Bacillus licheniformis β-lactamases: multiple forms and their roles
CA2419461A1 (fr) Micro-organismes et essais pour l'identification d'antibiotiques
DE60207157T2 (de) Reinigungsverfahren auf Grundlage der Bindung von Enzymen and markierte Peptide

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: B1

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: B1

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 09647867

Country of ref document: US

NENP Non-entry into the national phase in:

Ref country code: KR

WWE Wipo information: entry into national phase

Ref document number: 1999915903

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999915903

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 1999915903

Country of ref document: EP