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WO1999043287A3 - Phosphate-based dendrimers for bioassays - Google Patents

Phosphate-based dendrimers for bioassays Download PDF

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Publication number
WO1999043287A3
WO1999043287A3 PCT/US1999/004068 US9904068W WO9943287A3 WO 1999043287 A3 WO1999043287 A3 WO 1999043287A3 US 9904068 W US9904068 W US 9904068W WO 9943287 A3 WO9943287 A3 WO 9943287A3
Authority
WO
WIPO (PCT)
Prior art keywords
bioreagents
moiety
bioassays
phosphate
generations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/004068
Other languages
French (fr)
Other versions
WO1999043287A2 (en
Inventor
Andrzej Wilk
Andrzej K Drukier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotraces Inc
Original Assignee
Biotraces Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotraces Inc filed Critical Biotraces Inc
Priority to AU27880/99A priority Critical patent/AU2788099A/en
Publication of WO1999043287A2 publication Critical patent/WO1999043287A2/en
Publication of WO1999043287A3 publication Critical patent/WO1999043287A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/534—Production of labelled immunochemicals with radioactive label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A new class of bioreagents is based on 'closed surface' dendrimers comprising a linker arm, a forking moiety and a reporter moiety that can conjugate to a label and to a biomolecule. The bioreagents include spherical polymers where polymerization is controlled in order to achieve step-wise propagation, and thus a preselected number of reporter groups (preferably amines) on the surface. Products of this step-growth are called generations, and the number of amine groups is doubled with each subsequent generation. For the present invention the range of dendrimer generations is about 3-20, and the preferred range is 8-12.
PCT/US1999/004068 1998-02-25 1999-02-25 Phosphate-based dendrimers for bioassays Ceased WO1999043287A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU27880/99A AU2788099A (en) 1998-02-25 1999-02-25 Phosphate-based dendrimers for bioassays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7585998P 1998-02-25 1998-02-25
US60/075,859 1998-02-25

Publications (2)

Publication Number Publication Date
WO1999043287A2 WO1999043287A2 (en) 1999-09-02
WO1999043287A3 true WO1999043287A3 (en) 2000-04-06

Family

ID=22128433

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/004068 Ceased WO1999043287A2 (en) 1998-02-25 1999-02-25 Phosphate-based dendrimers for bioassays

Country Status (2)

Country Link
AU (1) AU2788099A (en)
WO (1) WO1999043287A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003503591A (en) * 1999-06-29 2003-01-28 ダコ エー エス Detection using dendrimers carrying labels and probes
EP1136569A3 (en) * 2000-03-24 2004-01-28 Bayer Corporation Nucleic acid probes having highly hydrophilic non-nucleosidic tags with multiple labels, and uses thereof
KR100377946B1 (en) * 2000-07-15 2003-03-29 한국과학기술원 A Process for Preparing Monolayer by Using Dendrimer
KR100379720B1 (en) * 2000-10-14 2003-04-11 주식회사 마크로젠 Supporter containing drimer monolayer and manufacturing method of same
WO2001081924A2 (en) * 2001-04-23 2001-11-01 Biotraces, Inc. Reusable microarrays for quantifying low abundance proteins
US9201067B2 (en) * 2003-03-05 2015-12-01 Posco Size-controlled macromolecule
JP5095764B2 (en) * 2003-09-18 2012-12-12 ポスコ Substrate, manufacturing method, diagnostic system and detection method
US8563329B2 (en) 2005-05-02 2013-10-22 Anp Technologies, Inc. Polymer conjugate enhanced bioassays
US20110014151A1 (en) * 2006-01-11 2011-01-20 Biotech Igg Ab Macromolecule conjugate
JP6762296B2 (en) * 2014-10-02 2020-09-30 ヴェンタナ メディカル システムズ， インク． Polymers and conjugates containing polymers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001072A (en) * 1984-05-23 1991-03-19 Icn Biomedicals Inc. Compositions and methods for multiple simultaneous immunoradiometric assay (IRMA) of analytes using radioisotope chelate labels
US5435990A (en) * 1988-06-24 1995-07-25 The Dow Chemical Company Macrocyclic congugates and their use as diagnostic and therapeutic agents
US5527524A (en) * 1986-08-18 1996-06-18 The Dow Chemical Company Dense star polymer conjugates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001072A (en) * 1984-05-23 1991-03-19 Icn Biomedicals Inc. Compositions and methods for multiple simultaneous immunoradiometric assay (IRMA) of analytes using radioisotope chelate labels
US5527524A (en) * 1986-08-18 1996-06-18 The Dow Chemical Company Dense star polymer conjugates
US5435990A (en) * 1988-06-24 1995-07-25 The Dow Chemical Company Macrocyclic congugates and their use as diagnostic and therapeutic agents

Also Published As

Publication number Publication date
WO1999043287A2 (en) 1999-09-02
AU2788099A (en) 1999-09-15

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